Title:
MODIFIED AMADORIASE CAPABLE OF ACTING ON FRUCTOSYL HEXAPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2013/162035
Kind Code:
A1
Abstract:
Provided is an amadoriase which has high reactivity with an α-fructosyl hexapeptide (αF6P) originated from the β-chain amino terminal of glycated hemoglobin (HbA1c) and enables the satisfactory quantification of an αF6P that has been cleaved from HbA1c. A novel amadoriase produced by substituting at least one amino acid residue at a position corresponding to an amino acid residue selected from the group consisting of amino acid residues located at position-62, position-63, position-102, position-106, position-110, position-113, position-355 and position-419 in an amadoriase originated from a bacterium belonging to the genus Coniochaeta. The amadoriase enables the rapid, simple, highly accurate and satisfactory quantification of an αF6P that has been cleaved from HbA1c. Using the amadoriase, it is possible to provide: an HbA1c measurement method by an enzymatic method, which is based on the same principle as that in a conventional reference method and is superior with respect to the correlation with a reference method; and an HbA1c measurement kit.
Inventors:
ICHIYANAGI ATSUSHI (JP)
MASAKARI YOSUKE (JP)
MASAKARI YOSUKE (JP)
Application Number:
PCT/JP2013/062508
Publication Date:
October 31, 2013
Filing Date:
April 26, 2013
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12Q1/48
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| WO2012018094A1 | 2012-02-09 | |||
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HIROKAWA K ET AL.: "Enzymes used for the determination of HbAlC", FEMS MICROBIOL. LETT., vol. 235, no. 1, 2004, pages 157 - 162, XP002903440
ATSUSHI ICHIYANAGI ET AL.: "Coniochaeta sp. NISL9330 Yurai Fructosyl peptide oxidase no Kishitsu Tokuisei no Kaihen", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU, vol. 2012, 5 March 2012 (2012-03-05), pages 4CO9A04, XP008175387
HIROKAWA K ET AL.: "An enzymatic method for the determination of hemoglobinA(lC)", BIOTECHNOL. LETT., vol. 27, no. 14, 2005, pages 963 - 968, XP019230894
JEPPSSON JO ET AL.: "Approved IFCC reference method for the measurement of HbAlc in human blood", CLIN. CHEM. LAB. MED., vol. 40, no. 1, 2002, pages 78 - 89, XP002378412
HIROKAWA K ET AL.: "Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 311, no. 1, 2003, pages 104 - 111, XP004465110
ATSUSHI ICHIYANAGI ET AL.: "Tonyobyo Shindan ni Tekishita Fructosyl peptide oxidase no Kaihatsu", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU, vol. 2013, 5 March 2013 (2013-03-05), pages 3C15A06, XP008175380
BIOCHEM. BIOPHYS. RES. COMMUN., vol. 311, 2003, pages 104 - 11
BIOTECHNOL. BIOENG., vol. 106, 2010, pages 358 - 66
J. BIOSCI. BIOENG., vol. 102, 2006, pages 241 - 3
APPL. MICROBIOL. BIOTECHNOL., vol. 74, 2007, pages 813 - 9
EUR. J. BIOCHEM., vol. 242, 1996, pages 499 - 505
MAR. BIOTECHNOL., vol. 6, 2004, pages 625 - 32
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 66, 2002, pages 1256 - 61
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 66, 2002, pages 2323 - 29
BIOTECHNOL. LETTERS, vol. 27, 2005, pages 27 - 32
JEPPSSON JO ET AL.: "Approved IFCC reference method for the measurement of HbAlc in human blood", CLIN. CHEM. LAB. MED., vol. 40, 2002, pages 78 - 89, XP002378412, DOI: doi:10.1515/CCLM.2002.016
SAMBROOK ET AL.: "Molecular Cloning", vol. 2, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987
"Current Protocols in Molecular Biology", 1989, WILEY INTERSCIENCE
GENDAI KAGAKU, June 1989 (1989-06-01), pages 24 - 30
NUCLEIC ACIDS RES., vol. 12, 1984, pages 9441
METHODS ENZYMOL., vol. 154, 1987, pages 350
GENE, vol. 37, 1985, pages 73
NUCLEIC ACIDS RES., vol. 13, 1985, pages 8749
NUCLEIC ACIDS RES., vol. 13, 1985, pages 8765
NUCLEIC ACIDS RES, vol. 14, 1986, pages 9679
PROC. NATL. ACID. SCI. U.S.A., vol. 82, 1985, pages 488
METHODS ENZYMOL., vol. 154, 1987, pages 367
TECHNIQUE, vol. 1, 1989, pages 11
CLIN. CHEM., vol. 43, 1997, pages 1994 - 1951
BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, 2010, pages 358 - 366
PROTEIN SCI., vol. 4, 1995, pages 2411 - 23
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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