Title:
MODIFIED AMINOTRANSFERASE, GENE THEREOF, AND METHOD FOR PRODUCING OPTICALLY ACTIVE AMINO COMPOUND USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/124639
Kind Code:
A1
Abstract:
Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having amino transferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an amino transferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
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Inventors:
ITO NORIYUKI (JP)
KAWANO SHIGERU (JP)
YASOHARA YOSHIHIKO (JP)
KAWANO SHIGERU (JP)
YASOHARA YOSHIHIKO (JP)
Application Number:
PCT/JP2012/056174
Publication Date:
September 20, 2012
Filing Date:
March 09, 2012
Export Citation:
Assignee:
KANEKA CORP (JP)
ITO NORIYUKI (JP)
KAWANO SHIGERU (JP)
YASOHARA YOSHIHIKO (JP)
ITO NORIYUKI (JP)
KAWANO SHIGERU (JP)
YASOHARA YOSHIHIKO (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12P13/00; C12P13/04; C12P41/00
Domestic Patent References:
| WO2007139055A1 | 2007-12-06 | |||
| WO2006126498A1 | 2006-11-30 | |||
| WO2006126498A1 | 2006-11-30 | |||
| WO2007139255A1 | 2007-12-06 | |||
| WO1994003613A1 | 1994-02-17 | |||
| WO2006126498A1 | 2006-11-30 | |||
| WO2007139055A1 | 2007-12-06 | |||
| WO2007093372A1 | 2007-08-23 |
Foreign References:
| US20090117627A1 | 2009-05-07 | |||
| US20080213845A1 | 2008-09-04 |
Other References:
NORIYUKI ITO ET AL.: "Pseudomonas fluorescens Yurai no Shinki Amino Ki Ten'i Koso Idenshi no Cloning to Gai Koso o Mochiita Kogaku Kassei Amine Rui no Gosei", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2009 NENDO (HEISEI 21 NENDO) TAIKAI KOEN YOSHISHU, 5 May 2009 (2009-05-05), pages 122, XP008167192
NORIYUKI ITO ET AL.: "Amino Ki Ten'i Koso o Mochiita Kogaku Kassei Amine Rui no Koritsuteki na Gosei", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2010 NENDO (HEISEI 22 NENDO) TAIKAI KOEN YOSHISHU, 5 May 2012 (2012-05-05), pages 93, XP008167194
TRENDS IN BIOTECHNOLOGY, vol. 28, 2010, pages 324 - 332
"Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", GREENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
JOSEPH SAMBROOK: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
BIOTECHNOLOGY AND BIOENGINEERING, vol. 99, no. 2, 2008, pages 275 - 284
LEUNG ET AL., TECHNIQUE, vol. 1, 1989, pages 11 - 15
OLFERT LANDT ET AL., GENE, vol. 96, 1990, pages 125 - 128
SMITH ET AL.: "Genetic Engineering", vol. 3 1, A PLENUM
VLASUK; INOUYE, M. ET AL.: "Experimental Manipulation of Gene Expression", ACADEMIC PRESS
HOS.N.HUNT ET AL., GENE, vol. 77, 1989, pages 51
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Biseibutsugaku Kiso Koza, 8, Idensikogaku", 1987, KYORITSU SHUPPAN
NATURE, vol. 315, 1985, pages 592 - 594
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 72, no. 11, 2008, pages 3030 - 3033
NORIYUKI ITO ET AL.: "Amino Ki Ten'i Koso o Mochiita Kogaku Kassei Amine Rui no Koritsuteki na Gosei", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2010 NENDO (HEISEI 22 NENDO) TAIKAI KOEN YOSHISHU, 5 May 2012 (2012-05-05), pages 93, XP008167194
TRENDS IN BIOTECHNOLOGY, vol. 28, 2010, pages 324 - 332
"Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", GREENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
JOSEPH SAMBROOK: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
BIOTECHNOLOGY AND BIOENGINEERING, vol. 99, no. 2, 2008, pages 275 - 284
LEUNG ET AL., TECHNIQUE, vol. 1, 1989, pages 11 - 15
OLFERT LANDT ET AL., GENE, vol. 96, 1990, pages 125 - 128
SMITH ET AL.: "Genetic Engineering", vol. 3 1, A PLENUM
VLASUK; INOUYE, M. ET AL.: "Experimental Manipulation of Gene Expression", ACADEMIC PRESS
HOS.N.HUNT ET AL., GENE, vol. 77, 1989, pages 51
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Biseibutsugaku Kiso Koza, 8, Idensikogaku", 1987, KYORITSU SHUPPAN
NATURE, vol. 315, 1985, pages 592 - 594
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 72, no. 11, 2008, pages 3030 - 3033
Attorney, Agent or Firm:
Yamada, Iichiro et al. (JP)
Yamada 威 1 郎 (JP)
Yamada 威 1 郎 (JP)
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