Title:
MODIFIED ANTIBODY AGAINST CD22 AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2004/087763
Kind Code:
A1
Abstract:
Based on the sequencial data of two anti-CD22 antibodies having been published, CD22 diabodies in which variable regions of the heavy chain and the light chain are bonded together via a 5mer linker are constructed. These 2 diabodies are examined in binding to lymphoma cells and activity of inducing cell death (apoptosis). As a result, it is found out that both of these diabodies bind to a Raji celle (i.e., a B lymphoma cell line) and have an activity of inducing apoptosis in the Raji cell and a Daudi cell which is also a B lymphoma cell line. These results indicate that degraded antibodies recognizing CD22 are usable as apoptosis inducers in tumor cells such as lymphocyte cells.
Inventors:
TSUCHIYA MASAYUKI (JP)
KIMURA NAOKI (JP)
FUKUDA TATSUYA (JP)
KIMURA NAOKI (JP)
FUKUDA TATSUYA (JP)
Application Number:
PCT/JP2004/004696
Publication Date:
October 14, 2004
Filing Date:
March 31, 2004
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
TSUCHIYA MASAYUKI (JP)
KIMURA NAOKI (JP)
FUKUDA TATSUYA (JP)
TSUCHIYA MASAYUKI (JP)
KIMURA NAOKI (JP)
FUKUDA TATSUYA (JP)
International Classes:
A61P1/04; A61P1/16; A61P3/10; A61P5/14; A61P7/00; A61P7/04; A61P7/06; A61P11/06; A61P15/08; A61P17/00; A61P17/06; A61P21/04; A61P25/00; A61P27/02; A61P29/00; A61P35/00; A61P35/02; A61P37/02; A61P43/00; C07K16/28; (IPC1-7): C07K16/28; A61K39/395; A61P1/04; A61P1/16; A61P3/10; A61P5/14; A61P7/00; A61P7/04; A61P7/06; A61P11/06; A61P15/08; A61P17/00; A61P17/06; A61P21/04; A61P25/00; A61P27/02; A61P29/00; A61P35/00; A61P35/02; A61P37/02; A61P43/00; C12N15/62
Domestic Patent References:
| WO2001097858A2 | 2001-12-27 | |||
| WO2002022212A2 | 2002-03-21 | |||
| WO2001074388A1 | 2001-10-11 | |||
| WO2002004021A1 | 2002-01-17 | |||
| WO2003033654A2 | 2003-04-24 |
Foreign References:
| JP2001518930A | 2001-10-16 | |||
| JP2002544173A | 2002-12-24 | |||
| JPH10505231A | 1998-05-26 |
Other References:
HOLLIGER P. ET AL: ""Diabodies": small bivalent and bispecific antibody fragments.", PROC NATL ACAD SCI U S A, vol. 14, no. 90, 1993, USA, pages 6444 - 6448, XP002008022
See also references of EP 1609803A4
See also references of EP 1609803A4
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-mach, Tsuchiura-shi Ibaraki, JP)
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