Title:
MODIFIED FAB REGION-BINDING PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2016/136910
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a modified Fab region-binding peptide that exhibits excellent selective adsorption ability to the Fab region of IgG, while also allowing proteins containing the Fab region to be discharged by using a relatively high-pH acidic elution solution. Another purpose of the present invention is to provide an affinity separation matrix having the modified Fab region-binding peptide as a ligand, a method for producing a Fab region-containing protein using the affinity separation matrix, DNA coding for the modified Fab region-binding peptide, a vector comprising the DNA, and a transformant transformed by the vector. The problem is solved by making use of a protein G variant having an amino acid substitution mutation at a specific position.
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Inventors:
YOSHIDA SHINICHI (JP)
Application Number:
PCT/JP2016/055701
Publication Date:
September 01, 2016
Filing Date:
February 25, 2016
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C07K14/315; B01D15/08; B01J20/22; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; G01N30/88
Domestic Patent References:
| WO2014021240A1 | 2014-02-06 | |||
| WO2015030094A1 | 2015-03-05 |
Foreign References:
| JP2015003872A | 2015-01-08 | |||
| JP2009195184A | 2009-09-03 |
Attorney, Agent or Firm:
UEKI, Kyuichi et al. (JP)
Hisakazu Ueki (JP)
Hisakazu Ueki (JP)
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