Title:
MODIFIED FC FRAGMENT, ANTIBODY COMPRISING SAME, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/168554
Kind Code:
A1
Abstract:
The present invention provides a modified Fc fragment, an antibody comprising the same, and an application thereof. The Fc fragment is derived from human IgG1, and the CH2 domain of the constant region of the Fc fragment comprises multiple substitutions. The substitutions reduce the binding ability of the Fc fragment to an Fcγ receptor (FcγR), and reduce non-specific T-cell activation induced by an antibody, such as a CD3 antibody.
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Inventors:
ZHANG JING (CN)
FANG LIJUAN (CN)
YAN YONGXIANG (CN)
ZENG LIANG (CN)
ZHOU PENGFEI (CN)
FANG LIJUAN (CN)
YAN YONGXIANG (CN)
ZENG LIANG (CN)
ZHOU PENGFEI (CN)
Application Number:
PCT/CN2019/075881
Publication Date:
August 27, 2020
Filing Date:
February 22, 2019
Export Citation:
Assignee:
WUHAN YZY BIOPHARMA CO LTD (CN)
International Classes:
C07K16/28
Domestic Patent References:
| WO2007024249A2 | 2007-03-01 | |||
| WO2010065578A2 | 2010-06-10 | |||
| WO2013096221A1 | 2013-06-27 | |||
| WO2017052321A1 | 2017-03-30 | |||
| WO2009100309A2 | 2009-08-13 | |||
| WO2011066501A1 | 2011-06-03 | |||
| WO1991009967A1 | 1991-07-11 |
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| US20130078249A1 | 2013-03-28 | |||
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Other References:
CURR OPIN BIOTECHNOL, vol. 22, no. 6, December 2011 (2011-12-01), pages 858 - 67
PROTEIN ENG DES SEL., vol. 29, no. 10, October 2016 (2016-10-01), pages 457 - 466
J IMMUNOL, vol. 170, 2003, pages 3134 - 3138
"Current Protocols in Molecular Biology", 2007
HAMERS-CASTERMAN ET AL., NATURE, vol. 363, 1993, pages 446 - 448
KABAT, E. ET AL.: "Sequences of Proteins of Immunological Interest", 1983, U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
CHOTHIA ET AL., J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
ROUX ET AL., J. IMMUNOL, vol. 161, 1998, pages 4083
ARNON ET AL.: "Monoclonal Antibodies And Cancer Therapy", 1985, ALAN R. LISS, article "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy", pages: 243 - 56
HELLSTROM ET AL.: "Controlled Drug Delivery", 1987, MARCEL DEKKER, article "Antibodies For Drug Delivery", pages: 623 - 53
THORPE ET AL.: "Monoclonal Antibodies '84: Biological And Clinical Applications", 1985, article "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review", pages: 475 - 506
"Monoclonal Antibodies For Cancer Detection And Therapy", 1985, ACADEMIC PRESS, article "Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy", pages: 303 - 16
THORPE ET AL.: "Immunol. Rev.", vol. 52, 1982, article "The Preparation And Cytotoxic Properties Of Antibody-Toxin Conjugates", pages: 119 - 58
SKERRA ET AL., SCIENCE, vol. 240, 1988, pages 1038 - 1040
HUSTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 55, 1988, pages 5879 - 5883
WARD ET AL., NATURE, vol. 334, 1989, pages 544 - 554
HUSTON ET AL., METHODS IN ENZYMOLOGY, vol. 203, 1991, pages 46 - 88
SHU ET AL., PROC. NATL. SCI. USA, vol. 90, 1993, pages 1995 - 1999
MORRISON, SCIENCE, vol. 229, 1985, pages 1202
OI ET AL., BIOTECHNIQUES, vol. 4, 1986, pages 214
GILLIES ET AL., J. IMMUNOL. METHODS, vol. 125, 1989, pages 191 - 202
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323
PADLAN, MOLECULAR IMMUNOLOGY, vol. 28, no. 4-5, 1991, pages 489 - 498
STUDNICKA ET AL., PROTEIN ENGINEERING, vol. 7, no. 6, 1994, pages 805 - 814
ROGUSKA ET AL., PROC. NATL. SCI. USA, vol. 91, 1994, pages 969 - 973
CHOTHIA ET AL., J. MOL. BIOL., vol. 278, 1998, pages 457 - 479
MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, 1984, pages 851 - 855
NEUBERGER ET AL., NATURE, vol. 372, 1984, pages 604 - 608
TAKEDA ET AL., NATURE, vol. 314, 1985, pages 452 - 454
NEWMAN, BIOTECHNOLOGY, vol. 10, 1992, pages 1455 - 1460
"Current Protocols in Immunology", 1991, JOHN WILEY AND SONS
RIDGWAY ET AL.: "Knob-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization", PROTEIN ENGINEERING, vol. 9, no. 7, 1996, pages 617 - 21
See also references of EP 3929212A4
PROTEIN ENG DES SEL., vol. 29, no. 10, October 2016 (2016-10-01), pages 457 - 466
J IMMUNOL, vol. 170, 2003, pages 3134 - 3138
"Current Protocols in Molecular Biology", 2007
HAMERS-CASTERMAN ET AL., NATURE, vol. 363, 1993, pages 446 - 448
KABAT, E. ET AL.: "Sequences of Proteins of Immunological Interest", 1983, U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
CHOTHIA ET AL., J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
ROUX ET AL., J. IMMUNOL, vol. 161, 1998, pages 4083
ARNON ET AL.: "Monoclonal Antibodies And Cancer Therapy", 1985, ALAN R. LISS, article "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy", pages: 243 - 56
HELLSTROM ET AL.: "Controlled Drug Delivery", 1987, MARCEL DEKKER, article "Antibodies For Drug Delivery", pages: 623 - 53
THORPE ET AL.: "Monoclonal Antibodies '84: Biological And Clinical Applications", 1985, article "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review", pages: 475 - 506
"Monoclonal Antibodies For Cancer Detection And Therapy", 1985, ACADEMIC PRESS, article "Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy", pages: 303 - 16
THORPE ET AL.: "Immunol. Rev.", vol. 52, 1982, article "The Preparation And Cytotoxic Properties Of Antibody-Toxin Conjugates", pages: 119 - 58
SKERRA ET AL., SCIENCE, vol. 240, 1988, pages 1038 - 1040
HUSTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 55, 1988, pages 5879 - 5883
WARD ET AL., NATURE, vol. 334, 1989, pages 544 - 554
HUSTON ET AL., METHODS IN ENZYMOLOGY, vol. 203, 1991, pages 46 - 88
SHU ET AL., PROC. NATL. SCI. USA, vol. 90, 1993, pages 1995 - 1999
MORRISON, SCIENCE, vol. 229, 1985, pages 1202
OI ET AL., BIOTECHNIQUES, vol. 4, 1986, pages 214
GILLIES ET AL., J. IMMUNOL. METHODS, vol. 125, 1989, pages 191 - 202
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323
PADLAN, MOLECULAR IMMUNOLOGY, vol. 28, no. 4-5, 1991, pages 489 - 498
STUDNICKA ET AL., PROTEIN ENGINEERING, vol. 7, no. 6, 1994, pages 805 - 814
ROGUSKA ET AL., PROC. NATL. SCI. USA, vol. 91, 1994, pages 969 - 973
CHOTHIA ET AL., J. MOL. BIOL., vol. 278, 1998, pages 457 - 479
MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, 1984, pages 851 - 855
NEUBERGER ET AL., NATURE, vol. 372, 1984, pages 604 - 608
TAKEDA ET AL., NATURE, vol. 314, 1985, pages 452 - 454
NEWMAN, BIOTECHNOLOGY, vol. 10, 1992, pages 1455 - 1460
"Current Protocols in Immunology", 1991, JOHN WILEY AND SONS
RIDGWAY ET AL.: "Knob-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization", PROTEIN ENGINEERING, vol. 9, no. 7, 1996, pages 617 - 21
See also references of EP 3929212A4
Attorney, Agent or Firm:
CHINA SCIENCE PATENT & TRADEMARK AGENT LTD. (CN)
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