Title:
MODIFIED FIBROIN
Document Type and Number:
WIPO Patent Application WO/2019/022163
Kind Code:
A1
Abstract:
The present invention relates to modified fibroin containing a domain sequence represented by formula 1: [((A)n motif)-REP]m or formula 2: [((A)n motif)-REP]m-((A)n motif), wherein the domain sequence has an amino acid sequence having a reduced glutamine residue content in association with the deletion of one or multiple glutamine residues in REP or the substitution of one or multiple glutamine residues in REP by another amino acid residues. [In formula 1 and formula 2, "(A)n motif" represents an amino acid sequence composed of 4 to 27 amino acid residues, wherein the ratio of the number of alanine residues relative to the total number of amino acid residues in "(A)n motif" is 80% or more; "REP" represents an amino acid residue composed of 10 to 200 amino acid residues; m represents an integer of 10 to 300; and multiple pieces of "(A)n motifs" or the like may have the same amino acid sequence as each other or may have different amino acid sequences from each other.]
Inventors:
MORITA KEISUKE (JP)
ABE YUNOSUKE (JP)
MAEKAWA TAKEHISA (JP)
KOTAKA KOICHI (JP)
SUGAHARA JUNICHI (JP)
NUMATA KEIJI (JP)
ABE YUNOSUKE (JP)
MAEKAWA TAKEHISA (JP)
KOTAKA KOICHI (JP)
SUGAHARA JUNICHI (JP)
NUMATA KEIJI (JP)
Application Number:
PCT/JP2018/027974
Publication Date:
January 31, 2019
Filing Date:
July 25, 2018
Export Citation:
Assignee:
SPIBER INC (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C07K14/435; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/12; D01F4/02
Domestic Patent References:
| WO2011113592A1 | 2011-09-22 | |||
| WO2017222034A1 | 2017-12-28 | |||
| WO2015042164A2 | 2015-03-26 | |||
| WO1994023021A1 | 1994-10-13 |
Foreign References:
| JP2013506058A | 2013-02-21 | |||
| JP2012055269A | 2012-03-22 | |||
| JP2005502347A | 2005-01-27 | |||
| JP2009505668A | 2009-02-12 | |||
| JP2014502140A | 2014-01-30 | |||
| JPH026869B2 | 1990-02-14 | |||
| JPH06294068A | 1994-10-21 | |||
| JPS63248394A | 1988-10-14 | |||
| JPS58110600A | 1983-07-01 | |||
| JPS60221091A | 1985-11-05 | |||
| US4686191A | 1987-08-11 | |||
| US4939094A | 1990-07-03 | |||
| US5160735A | 1992-11-03 | |||
| JPH0231682A | 1990-02-01 | |||
| JPH04278091A | 1992-10-02 | |||
| JPH07170984A | 1995-07-11 | |||
| JPH06133782A | 1994-05-17 | |||
| JP2002238569A | 2002-08-27 | |||
| JPH02227075A | 1990-09-10 | |||
| JPH05336963A | 1993-12-21 | |||
| JP5678283B2 | 2015-02-25 | |||
| JP4638735B2 | 2011-02-23 |
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APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1994, pages 358 - 363
MICROBIAL CELL FACTORIES, vol. 3, 2004, pages 14
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KYTE JDOOLITTLE R: "A simple method for displaying the hydropathic character of a protein", J. MOL. BIOL., vol. 157, 1982, pages 105 - 132, XP024014365, DOI: 10.1016/0022-2836(82)90515-0
"GenBank", Database accession no. AAC 14589.1
NUCLEIC ACID RES., vol. 10, 1982, pages 6487
METHODS IN ENZYMOLOGY, vol. 100, 1983, pages 448
COHEN ET AL.: "calcium chloride method", PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
GENE, vol. 17, 1982, pages 107
MOLECULAR & GENERAL GENETICS, vol. 168, 1979, pages 111
TAKAHASHI ET AL., J. BACTERIOL., vol. 153, 1983, pages 1130 - 1134
TAKAGI ET AL., AGRIC. BIOL. CHEM., vol. 53, 1989, pages 3099 - 3100
OKAMOTO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 61, 1997, pages 202 - 203
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 669
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 4306
J. BACTERIOL., vol. 172, 1990, pages 2392
J. BACTERIOL., 1987, pages 1239 - 1245
UDAKA SHIGEZOU, JOURNAL OF THE AGRICULTURAL CHEMICAL SOCIETY OF JAPAN, vol. 61, 1987, pages 669 - 676
APPL. MICROBIOL. BIOTECHNOL., vol. 30, 1989, pages 75 - 80
BIOCHEM., vol. 112, 1992, pages 488 - 491
APPL. ENVIRON. MICROBIOL., vol. 58, 1992, pages 525 - 531
J. BACTERIOL., vol. 177, 1995, pages 745 - 749
MYERS, A. M. ET AL., GENE, vol. 45, 1986, pages 299 - 310
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI., vol. 81, 1984, pages 4889
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
MOL. GEN. GENET., vol. 168, 1979, pages 111
J. MOL. BIOL., vol. 56, 1971, pages 209
"Current Protocols in Molecular Biology, Baculovirus Expression Vectors", 1992, A LABORATORY MANUAL, W. H. FREEMAN AND COMPANY
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
PROC. NATL. ACAD. SCI. USA, vol. 84, 1987, pages 7413
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, 1995, pages 1795 - 1797
ENZYME MICROBIOL TECHNOL, vol. 6, 1984, pages 386 - 389
GENE, vol. 84, 1989, pages 329 - 334
BIOSCIBIOTECHNOL BIOCHEM, vol. 64, 2000, pages 1416 - 1421
BIOCHEMBIOPHYS RES COMMUN, vol. 112, 1983, pages 284 - 289
GENE, vol. 26, 1983, pages 205 - 221
MOL GEN GENET, vol. 261, 1999, pages 290 - 296
PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4869 - 4873
GENE, vol. 57, 1987, pages 21 - 26
NATURE, vol. 195, 1962, pages 788
PAULSON ET AL., J. BIOL. CHEM., vol. 264, 1989, pages 17619
LOWE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 8227
GENES DEVELOP., vol. 4, 1990, pages 1288
See also references of EP 3660036A4
TEULE, FLORENCE ET AL.: "Modifications of spider silk sequences in an attempt to control the mechanical properties of the synt", J. MATER. SCI., vol. 42, 2007, pages 8974 - 8985, XP019528790, ISSN: 1573-4803
BLACKLEDGE, T. A. ET AL.: "How super is supercontraction? Persistent versus cyclic responses to humidity in spider dragline silk.", J. EXP. BIOL., vol. 212, 2009, pages 1981 - 1989, XP055566452
APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1994, pages 358 - 363
MICROBIAL CELL FACTORIES, vol. 3, 2004, pages 14
SCIENCE, vol. 295, 2002, pages 472 - 476
KYTE JDOOLITTLE R: "A simple method for displaying the hydropathic character of a protein", J. MOL. BIOL., vol. 157, 1982, pages 105 - 132, XP024014365, DOI: 10.1016/0022-2836(82)90515-0
"GenBank", Database accession no. AAC 14589.1
NUCLEIC ACID RES., vol. 10, 1982, pages 6487
METHODS IN ENZYMOLOGY, vol. 100, 1983, pages 448
COHEN ET AL.: "calcium chloride method", PROC. NATL. ACAD. SCI. USA, vol. 69, 1972, pages 2110
GENE, vol. 17, 1982, pages 107
MOLECULAR & GENERAL GENETICS, vol. 168, 1979, pages 111
TAKAHASHI ET AL., J. BACTERIOL., vol. 153, 1983, pages 1130 - 1134
TAKAGI ET AL., AGRIC. BIOL. CHEM., vol. 53, 1989, pages 3099 - 3100
OKAMOTO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 61, 1997, pages 202 - 203
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 669
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 4306
J. BACTERIOL., vol. 172, 1990, pages 2392
J. BACTERIOL., 1987, pages 1239 - 1245
UDAKA SHIGEZOU, JOURNAL OF THE AGRICULTURAL CHEMICAL SOCIETY OF JAPAN, vol. 61, 1987, pages 669 - 676
APPL. MICROBIOL. BIOTECHNOL., vol. 30, 1989, pages 75 - 80
BIOCHEM., vol. 112, 1992, pages 488 - 491
APPL. ENVIRON. MICROBIOL., vol. 58, 1992, pages 525 - 531
J. BACTERIOL., vol. 177, 1995, pages 745 - 749
MYERS, A. M. ET AL., GENE, vol. 45, 1986, pages 299 - 310
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI., vol. 81, 1984, pages 4889
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
MOL. GEN. GENET., vol. 168, 1979, pages 111
J. MOL. BIOL., vol. 56, 1971, pages 209
"Current Protocols in Molecular Biology, Baculovirus Expression Vectors", 1992, A LABORATORY MANUAL, W. H. FREEMAN AND COMPANY
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
PROC. NATL. ACAD. SCI. USA, vol. 84, 1987, pages 7413
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, 1995, pages 1795 - 1797
ENZYME MICROBIOL TECHNOL, vol. 6, 1984, pages 386 - 389
GENE, vol. 84, 1989, pages 329 - 334
BIOSCIBIOTECHNOL BIOCHEM, vol. 64, 2000, pages 1416 - 1421
BIOCHEMBIOPHYS RES COMMUN, vol. 112, 1983, pages 284 - 289
GENE, vol. 26, 1983, pages 205 - 221
MOL GEN GENET, vol. 261, 1999, pages 290 - 296
PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4869 - 4873
GENE, vol. 57, 1987, pages 21 - 26
NATURE, vol. 195, 1962, pages 788
PAULSON ET AL., J. BIOL. CHEM., vol. 264, 1989, pages 17619
LOWE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 8227
GENES DEVELOP., vol. 4, 1990, pages 1288
See also references of EP 3660036A4
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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