Title:
MODIFIED GALECTIN-9 PROTEIN
Document Type and Number:
WIPO Patent Application WO/2014/080703
Kind Code:
A1
Abstract:
Provided is a modified form of galectin-9, which has substantially the same physiological activity as that of wild-type galectin-9, also has excellent protease stability and solubility, and can be produced with high yield. The modified form of galectin-9 according to the present invention is a protein which comprises a NCRD and a CCRD as mentioned below, wherein each of the NCRD and the CCRD comprises an N-terminal domain and a C-terminal domain, and wherein the C-terminal of the NCRD and the N-terminal of the CCRD are bound to each other directly or indirectly. The NCRD is (N1) a peptide comprising the amino acid sequence represented by SEQ ID NO: 1. The N-terminal domain of the CCRD is (C-N1) a peptide comprising an amino acid sequence produced by deleting 1 to 17 amino acid residues in the amino acid sequence represented by SEQ ID NO: 3, and the C-terminal domain of the CCRD is (C-C1) a peptide comprising the amino acid sequence represented by SEQ ID NO: 5.
Inventors:
NISHI NOZOMU (JP)
ITOH AIKO (JP)
ITOH AIKO (JP)
Application Number:
PCT/JP2013/077514
Publication Date:
May 30, 2014
Filing Date:
October 09, 2013
Export Citation:
Assignee:
UNIV KAGAWA NAT UNIV CORP (JP)
International Classes:
C12N15/09; A61K31/7088; A61K35/76; A61K38/00; A61K48/00; A61P29/00; A61P37/02; A61P37/08; A61P43/00; C07K14/47; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Domestic Patent References:
| WO2005093064A1 | 2005-10-06 |
Other References:
NOZOMU NISHI ET AL.: "Development of highly stable galectins: Truncation of the linker peptide confers protease-resistance on tandem- repeat type galectins", FEBS LETTERS, vol. 579, April 2005 (2005-04-01), pages 2058 - 2064
TAEKO OKUDAIRA ET AL.: "A modified version of galectin-9 suppresses cell growth and induces apoptosis of human T-cell leukemia virus type I-infected T-cell lines", INT. J. CANCER, vol. 120, 2007, pages 2251 - 2261
HIROMI YOSHIDA ET AL.: "X-ray structures of human galectin-9 C-terminal domain in complexes with a biantennary oligosaccharide and sialyllactose", J. BIOL. CHEM., vol. 285, no. 47, 19 November 2010 (2010-11-19), pages 36969 - 36976
MIKI SATO ET AL.: "Functional analysis of the carbohydrate recognition domains and a linker peptide of galectin-9 as to eosinophil chemoattractant activity", GLYCOBIOLOGY, vol. 12, no. 3, 2002, pages 191 - 197
OEZLEM TUERECI ET AL.: "Molecular definition of a novel human galectin which is immunogenic in patients with Hodgkin's disease", J. BIOL. CHEM., vol. 272, no. 10, 7 March 1997 (1997-03-07), pages 6416 - 6422
AIKO ITOH ET AL.: "Optimization of the inter- domain structure of galectin-9 for recombinant production", GLYCOBIOLOGY, vol. 23, no. 8, August 2013 (2013-08-01), pages 920 - 925
TAEKO OKUDAIRA ET AL.: "A modified version of galectin-9 suppresses cell growth and induces apoptosis of human T-cell leukemia virus type I-infected T-cell lines", INT. J. CANCER, vol. 120, 2007, pages 2251 - 2261
HIROMI YOSHIDA ET AL.: "X-ray structures of human galectin-9 C-terminal domain in complexes with a biantennary oligosaccharide and sialyllactose", J. BIOL. CHEM., vol. 285, no. 47, 19 November 2010 (2010-11-19), pages 36969 - 36976
MIKI SATO ET AL.: "Functional analysis of the carbohydrate recognition domains and a linker peptide of galectin-9 as to eosinophil chemoattractant activity", GLYCOBIOLOGY, vol. 12, no. 3, 2002, pages 191 - 197
OEZLEM TUERECI ET AL.: "Molecular definition of a novel human galectin which is immunogenic in patients with Hodgkin's disease", J. BIOL. CHEM., vol. 272, no. 10, 7 March 1997 (1997-03-07), pages 6416 - 6422
AIKO ITOH ET AL.: "Optimization of the inter- domain structure of galectin-9 for recombinant production", GLYCOBIOLOGY, vol. 23, no. 8, August 2013 (2013-08-01), pages 920 - 925
Attorney, Agent or Firm:
TSUJIMARU Koichiro et al. (JP)
Koichiro Tsujimaru (JP)
Koichiro Tsujimaru (JP)
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