Title:
MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING 2- PROPANOL AND 1-PROPANOL AND/OR 1.2-PROPANEDIOL
Document Type and Number:
WIPO Patent Application WO/2015/035226
Kind Code:
A3
Abstract:
The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol.
Inventors:
SLOVIC AVRAM MICHAEL (BR)
PEREZ JOHANA RINCONES (BR)
ROJAS JUAN DIEGO ROJAS (BR)
ZEIDLER ANE FERNANDA BERALDI (BR)
DUMARESQ ALINE SILVA ROMAO (BR)
RODRIGUES MARILENE ELIZABETE PAVAN (BR)
GOUVEA IURI ESTRADA (BR)
GALZERANI FELIPE (BR)
KOCH DANIEL JOHANNES (BR)
PARIZZI LUCAS PEDERSEN (BR)
LOPES MATEUS SCHREINER GARCEZ (BR)
HALDER THOMAS MARTIN (BR)
MORSCHBACKER ANTONIO LUIS RIBEIRO DE CASTRO (BR)
KOLLING VERONICA MARIA RODEGE GOGOLA (BR)
PEREZ JOHANA RINCONES (BR)
ROJAS JUAN DIEGO ROJAS (BR)
ZEIDLER ANE FERNANDA BERALDI (BR)
DUMARESQ ALINE SILVA ROMAO (BR)
RODRIGUES MARILENE ELIZABETE PAVAN (BR)
GOUVEA IURI ESTRADA (BR)
GALZERANI FELIPE (BR)
KOCH DANIEL JOHANNES (BR)
PARIZZI LUCAS PEDERSEN (BR)
LOPES MATEUS SCHREINER GARCEZ (BR)
HALDER THOMAS MARTIN (BR)
MORSCHBACKER ANTONIO LUIS RIBEIRO DE CASTRO (BR)
KOLLING VERONICA MARIA RODEGE GOGOLA (BR)
Application Number:
PCT/US2014/054379
Publication Date:
October 15, 2015
Filing Date:
September 05, 2014
Export Citation:
Assignee:
BRASKEM SA (BR)
SLOVIC AVRAM MICHAEL (BR)
SLOVIC AVRAM MICHAEL (BR)
International Classes:
C12P7/14; C12N1/21; C12P7/00
Domestic Patent References:
| WO2012177599A2 | 2012-12-27 | |||
| WO2010051527A2 | 2010-05-06 | |||
| WO2012058603A1 | 2012-05-03 |
Foreign References:
| US20110039327A1 | 2011-02-17 |
Other References:
MORIYA, H ET AL.: "Glucose Sensing And Signaling In Saccharomyces cerevisiae Through The Rgt2 Glucose Sensor And Casein Kinase I.", PNAS., vol. 101, no. 6, 10 February 2004 (2004-02-10), pages 1572 - 1577, XP055230317, Retrieved from the Internet
Attorney, Agent or Firm:
BETTI, Christopher J. et al. (P.O. Box 1135Chicago, Illinois, US)
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