Title:
MODIFIED PROTEIN AND METHOD FOR USING SAME
Document Type and Number:
WIPO Patent Application WO/2019/159692
Kind Code:
A1
Abstract:
The present invention pertains to a means and method for, from a mixture (cell culture) of cells, a medium component, and a useful substance, recovering the first two using a single process. Specifically, the present invention pertains to a polypeptide modified by a stimuli-responsive polymer, characterized in that the stimuli-responsive polymer responds to stimuli to induce a change in the physical properties of the modified polypeptide overall, and the change is reversible or pseudo-reversible.
Inventors:
MARUYAMA MASASHI (JP)
SHIBUYA KEISUKE (JP)
SHIBUYA KEISUKE (JP)
Application Number:
PCT/JP2019/003123
Publication Date:
August 22, 2019
Filing Date:
January 30, 2019
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C07K14/00; C07K1/34; C08L89/00; C08L101/12; C12N1/00; C12P21/08; C07K14/62
Domestic Patent References:
| WO2018096881A1 | 2018-05-31 | |||
| WO2015064591A1 | 2015-05-07 |
Foreign References:
| JP2016054686A | 2016-04-21 |
Other References:
DING Z. ET AL.: "Synthesis and Purification of Thermally Sensitive Oligomer-Enzyme Conjugates of Poly (N-isopropylacrylamide)-Trypsin", BIOCONJUGATE CHEM., vol. 7, no. 1, 1996, pages 121 - 125, XP001007447, DOI: 10.1021/bc950087r
DING Z. ET AL.: "Unusual properties of thermally sensitive oligomer-enzyme conjugates of poly(N-isopropylacrylamide)-trypsin", J. BIOMED. MATER. RES., vol. 39, no. 3, 1998, pages 498 - 505, XP055633661
LI M. ET AL.: "Thermoresponsive Block Copolymer . Protein Conjugates Prepared by Grafting-from via RAFT Polymerization", MACROMOL. RAPID COMMUN., vol. 32, 2011, pages 354 - 359, XP055633671
TRZEBICKA B ET AL.: "Thermoresponsive polymer-peptide/protein conjugates", PROG. POLYM. SCI., vol. 68, 2016, pages 35 - 76, XP029985384, DOI: 10.1016/j.progpolymsci.2016.12.004
STUART M. A. C. ET AL.: "Emerging applications of stimuli-responsive polymer materials ,", NAT. MATER., vol. 9, 1 February 2010 (2010-02-01), pages 101 - 113, XP002698662
DING Z. ET AL.: "Unusual properties of thermally sensitive oligomer-enzyme conjugates of poly(N-isopropylacrylamide)-trypsin", J. BIOMED. MATER. RES., vol. 39, no. 3, 1998, pages 498 - 505, XP055633661
LI M. ET AL.: "Thermoresponsive Block Copolymer . Protein Conjugates Prepared by Grafting-from via RAFT Polymerization", MACROMOL. RAPID COMMUN., vol. 32, 2011, pages 354 - 359, XP055633671
TRZEBICKA B ET AL.: "Thermoresponsive polymer-peptide/protein conjugates", PROG. POLYM. SCI., vol. 68, 2016, pages 35 - 76, XP029985384, DOI: 10.1016/j.progpolymsci.2016.12.004
STUART M. A. C. ET AL.: "Emerging applications of stimuli-responsive polymer materials ,", NAT. MATER., vol. 9, 1 February 2010 (2010-02-01), pages 101 - 113, XP002698662
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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