Title:
MODIFIED PRP43 HELICASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/213253
Kind Code:
A1
Abstract:
A modified Prp43 helicase and use thereof. The ATP hydrolytic activity or unwinding activity of the Prp43 helicase is enhanced due to the introduction of mutation and/or the introduction of accessory proteins, and the binding to a target polynucleotide can be maintained for a long time, thereby allowing the enzyme to continuously and stably control the moving speed of the polynucleotide at a suitable rate required by sequencing, and can be used for nanopore sequencing.
Inventors:
ZHANG ZHOUGANG (CN)
LI WEN (CN)
WANG YANSHUANG (CN)
WANG MUYANG (CN)
LI WEN (CN)
WANG YANSHUANG (CN)
WANG MUYANG (CN)
Application Number:
PCT/CN2021/085609
Publication Date:
October 13, 2022
Filing Date:
April 06, 2021
Export Citation:
Assignee:
QITAN TECH LTD CHENGDU (CN)
International Classes:
C12N15/55; C12M1/34; C12N9/14; C12Q1/6869; G01N33/48
Domestic Patent References:
| WO2013057495A2 | 2013-04-25 |
Foreign References:
| CN112147185A | 2020-12-29 | |||
| CN109266537A | 2019-01-25 | |||
| CN108676785A | 2018-10-19 | |||
| US20150191709A1 | 2015-07-09 | |||
| US20150065354A1 | 2015-03-05 | |||
| US9617591B2 | 2017-04-11 | |||
| US20160257942A1 | 2016-09-08 | |||
| US20180179500A1 | 2018-06-28 |
Other References:
TAUCHERT MARCEL J, FOURMANN JEAN-BAPTISTE, LÜHRMANN REINHARD, FICNER RALF: "Structural insights into the mechanism of the DEAH-box RNA helicase Prp43", ELIFE, vol. 6, 16 January 2017 (2017-01-16), XP055976581, DOI: 10.7554/eLife.21510
TANAKA NAOKO, SCHWER BEATE: "Mutations in PRP43 That Uncouple RNA-Dependent NTPase Activity and Pre-mRNA Splicing Function", BIOCHEMISTRY, vol. 45, no. 20, 1 May 2006 (2006-05-01), pages 6510 - 6521, XP055976580, ISSN: 0006-2960, DOI: 10.1021/bi052656g
LONG JIAFU, ZHOU HAO, CHEN FEILONG: "Research progress in structure and function of polymerase-associated factor 1 complex", SCIENCE CHINA: CHINESE BULLETIN OF LIFE SCIENCE = SCIENTIA SINICA VITAE, vol. 49, no. 9, 1 September 2019 (2019-09-01), pages 1143 - 1154, XP055976785, ISSN: 1674-7232, DOI: 10.1360/SSV-2019-0154
NEEDLEMAN, S. B.WUNSCH, C. D., J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
MARCEL J. TAUCHERT ET AL.: "Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum", ACTA CRYST., vol. F72, 2016, pages 112 - 120, XP072457883, DOI: 10.1107/S2053230X15024498
SAMBROOKRUSSEL: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
F. AUSUBEL ET AL.: "Current protocols in Molecular biology", 1987, GREEN PUBLISHING AND WILEY INTERSCIENCE
MICHAEL FALLER ET AL.: "The Structure of a Mycobacterial Outer-Membrane Channel", SCIENCE, vol. 303, 2004, pages 1189, XP003029246, DOI: 10.1126/science.1094114
TANAKA NAOKO, SCHWER BEATE: "Mutations in PRP43 That Uncouple RNA-Dependent NTPase Activity and Pre-mRNA Splicing Function", BIOCHEMISTRY, vol. 45, no. 20, 1 May 2006 (2006-05-01), pages 6510 - 6521, XP055976580, ISSN: 0006-2960, DOI: 10.1021/bi052656g
LONG JIAFU, ZHOU HAO, CHEN FEILONG: "Research progress in structure and function of polymerase-associated factor 1 complex", SCIENCE CHINA: CHINESE BULLETIN OF LIFE SCIENCE = SCIENTIA SINICA VITAE, vol. 49, no. 9, 1 September 2019 (2019-09-01), pages 1143 - 1154, XP055976785, ISSN: 1674-7232, DOI: 10.1360/SSV-2019-0154
NEEDLEMAN, S. B.WUNSCH, C. D., J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
MARCEL J. TAUCHERT ET AL.: "Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum", ACTA CRYST., vol. F72, 2016, pages 112 - 120, XP072457883, DOI: 10.1107/S2053230X15024498
SAMBROOKRUSSEL: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
F. AUSUBEL ET AL.: "Current protocols in Molecular biology", 1987, GREEN PUBLISHING AND WILEY INTERSCIENCE
MICHAEL FALLER ET AL.: "The Structure of a Mycobacterial Outer-Membrane Channel", SCIENCE, vol. 303, 2004, pages 1189, XP003029246, DOI: 10.1126/science.1094114
Attorney, Agent or Firm:
BEIJING EAST IP LTD. (CN)
Download PDF:
Previous Patent: NONWOVEN FABRIC, PNEUMATIC TIRE INCLUDING SAME, AND METHOD OF MAKING PNEUMATIC TIRE
Next Patent: PARTIAL FREQUENCY SOUNDING
Next Patent: PARTIAL FREQUENCY SOUNDING