Title:
MODIFIED TAMAVIDIN
Document Type and Number:
WIPO Patent Application WO/2012/091110
Kind Code:
A1
Abstract:
The present invention provides, as a modified tamavidin 2, a modified biotin-binding protein which is characterized by being obtained by substituting the 115th asparagine residue in SEQ ID NO: 2 with cysteine in a protein that comprises the amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or several amino acid mutations in the amino acid sequence represented by SEQ ID NO: 2, or an amino acid sequence having 80% or more identity to the amino acid sequence represented by SEQ ID NO: 2 and that has biotin binding activity. The modified biotin-binding protein of the present invention has remarkable heat resistance.
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Inventors:
TAKAKURA YOSHIMITSU (JP)
OKA NAOMI (JP)
OKA NAOMI (JP)
Application Number:
PCT/JP2011/080451
Publication Date:
July 05, 2012
Filing Date:
December 28, 2011
Export Citation:
Assignee:
JAPAN TOBACCO INC (JP)
TAKAKURA YOSHIMITSU (JP)
OKA NAOMI (JP)
TAKAKURA YOSHIMITSU (JP)
OKA NAOMI (JP)
International Classes:
C12N15/00; C07K1/14; C07K14/375; C07K17/00; C12N15/09; G01N33/53; G01N33/68
Domestic Patent References:
| WO2010150375A1 | 2010-12-29 | |||
| WO2008081938A1 | 2008-07-10 | |||
| WO2009028625A1 | 2009-03-05 | |||
| WO2010018859A1 | 2010-02-18 | |||
| WO2002072817A1 | 2002-09-19 | |||
| WO2010018859A1 | 2010-02-18 |
Foreign References:
| JP2008200038A | 2008-09-04 |
Other References:
TAKAKURA Y. ET AL.: "Tamavidins - novel avidin-like biotin-binding proteins from the Tamogitake mushroom", FEES JOURNAL, vol. 276, no. 5, 2009, pages 1383 - 1397, XP002616627
TAKAKURA ET AL., FEBS J, vol. 276, 2009, pages 1383 - 1397
REZNIK ET AL., NAT. BIOTECHNOL., vol. 14, 1996, pages 1007 - 1011
NORDLUND ET AL., J. BIOL. CHEM., vol. 278, 2003, pages 2479 - 2483
HYTONEN ET AL., J. BIOL. CHEM., vol. 280, 2005, pages 10228 - 10233
TAKAKURA ET AL., FEBS J., vol. 276, 2009, pages 1383 - 1397
NUCLEIC ACID RESEARCH, vol. 10, no. 20, 1982, pages 6487 - 6500
NEEDLEMAN, S. B.; WUNSCH, C. D., J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
HENIKOFF, S.; HENIKOFF, J. G., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 10915 - 10919
ALTSCHUL, NUCL. ACIDS RES., vol. 25, 1997, pages 3389 - 3402
DEVEREUX ET AL., NUCL. ACIDS RES., vol. 12, 1984, pages 387
GRIBSKOV; BURGESS, NUCL. ACIDS RES., vol. 14, 1986, pages 6745
"Atlas of Polypeptide Sequence and Structure", 1979, NATIONAL BIOMEDICAL RESEARCH FOUNDATION, pages: 353 - 358
WOOTTON; FEDERHEN, COMPUTERS AND CHEMISTRY, 1993
WOOTTON; FEDERHEN: "Analysis of compositionally biased regions in sequence databases", METHODS ENZYMOL., vol. 266, 1996, pages 544 - 71
CLAVERIE; STATES, COMPUTERS AND CHEMISTRY, 1993
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
KADA ET AL., BIOCHIM. BIOPHYS. ACTA, vol. 1427, 1999, pages 33 - 43
HIGUCHI ET AL., NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
HO ET AL., GENE, vol. 77, 1989, pages 51 - 59
SAMBROOK ET AL.: "Molecular Cloning, A laboratory manual", 1989
TAKAKURA ET AL., FEBS J, vol. 276, 2009, pages 1383 - 1397
REZNIK ET AL., NAT. BIOTECHNOL., vol. 14, 1996, pages 1007 - 1011
NORDLUND ET AL., J. BIOL. CHEM., vol. 278, 2003, pages 2479 - 2483
HYTONEN ET AL., J. BIOL. CHEM., vol. 280, 2005, pages 10228 - 10233
TAKAKURA ET AL., FEBS J., vol. 276, 2009, pages 1383 - 1397
NUCLEIC ACID RESEARCH, vol. 10, no. 20, 1982, pages 6487 - 6500
NEEDLEMAN, S. B.; WUNSCH, C. D., J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
HENIKOFF, S.; HENIKOFF, J. G., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 10915 - 10919
ALTSCHUL, NUCL. ACIDS RES., vol. 25, 1997, pages 3389 - 3402
DEVEREUX ET AL., NUCL. ACIDS RES., vol. 12, 1984, pages 387
GRIBSKOV; BURGESS, NUCL. ACIDS RES., vol. 14, 1986, pages 6745
"Atlas of Polypeptide Sequence and Structure", 1979, NATIONAL BIOMEDICAL RESEARCH FOUNDATION, pages: 353 - 358
WOOTTON; FEDERHEN, COMPUTERS AND CHEMISTRY, 1993
WOOTTON; FEDERHEN: "Analysis of compositionally biased regions in sequence databases", METHODS ENZYMOL., vol. 266, 1996, pages 544 - 71
CLAVERIE; STATES, COMPUTERS AND CHEMISTRY, 1993
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
KADA ET AL., BIOCHIM. BIOPHYS. ACTA, vol. 1427, 1999, pages 33 - 43
HIGUCHI ET AL., NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
HO ET AL., GENE, vol. 77, 1989, pages 51 - 59
SAMBROOK ET AL.: "Molecular Cloning, A laboratory manual", 1989
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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