Title:
MODIFIED TRANSGLUTAMINASE
Document Type and Number:
WIPO Patent Application WO/2023/058765
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a modified transglutaminase that has improved reactivity in a heating temperature range. A transglutaminase, which comprises a polypeptide having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by: substitution of the combination of the three amino acid residues at the 249 to 251 positions by a combination of three predetermined amino acid residues; substitution of the combination of the three amino acid residues at the 243 to 245 positions by a combination of three predetermined amino acid residues; and/or substitution of the amino acid residue at the 2 position by a predetermined amino acid, has improved reactivity in a heating temperature range compared to the polypeptide having the amino acid sequence represented by SEQ ID NO: 1.
Inventors:
YOSHIDA KAZUNORI (JP)
UEDA KENTA (JP)
UEDA KENTA (JP)
Application Number:
PCT/JP2022/037716
Publication Date:
April 13, 2023
Filing Date:
October 07, 2022
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N15/54; A23L33/175; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12N15/63
Domestic Patent References:
| WO2010101256A1 | 2010-09-10 | |||
| WO2021177181A1 | 2021-09-10 | |||
| WO2019107288A1 | 2019-06-06 |
Foreign References:
| JP2021502068A | 2021-01-28 | |||
| CN111944778A | 2020-11-17 |
Other References:
MARX, C.K. ; HERTEL, T.C. ; PIETZSCH, M.: "Random mutagenesis of a recombinant microbial transglutaminase for the generation of thermostable and heat-sensitive variants", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 136, no. 3-4, 10 September 2008 (2008-09-10), Amsterdam NL , pages 156 - 162, XP024338589, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2008.06.005
MU DONGDONG, LU JIAOJIAO, SHU CHANG, LI HAOWEN, LI XINGJIANG, CAI JING, LUO SHUIZHONG, YANG PEIZHOU, JIANG SHAOTONG, ZHENG ZHI: "Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, JP, vol. 82, no. 1, 2 January 2018 (2018-01-02), JP , pages 106 - 109, XP055852155, ISSN: 0916-8451, DOI: 10.1080/09168451.2017.1403881
MU DONGDONG, LU JIAOJIAO, SHU CHANG, LI HAOWEN, LI XINGJIANG, CAI JING, LUO SHUIZHONG, YANG PEIZHOU, JIANG SHAOTONG, ZHENG ZHI: "Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, JP, vol. 82, no. 1, 2 January 2018 (2018-01-02), JP , pages 106 - 109, XP055852155, ISSN: 0916-8451, DOI: 10.1080/09168451.2017.1403881
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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