Title:
MODIFIED TREPONEMA PALLIDUM OUTER MEMBRANE PROTEIN, ITS IMMUNOASSAY USE AND IMMUNOASSAY KIT
Document Type and Number:
WIPO Patent Application WO/2000/047613
Kind Code:
A1
Abstract:
The present invention provides proteins of a general formula: (Xaa)nTpp(Xaa)m, wherein Tpp is selected from the outer membrane proteins of Treponema pallidum Tpp15, Tpp17 and Tpp47, and any immunoactive fragments and derivatives thereof that can bind to human immunoglobulins; Xaa represents a natural amino acid residue, wherein at least one of Xaas which are nearest to the two ends of Tpp and at least one terminal Xaa of the formula are selected from the group consisting of Lys, Arg and Trp; n and m represent independently an integer from 1 to 10, providing n+m$m(G)2. A novel method that assays anti-Treponema pallidum antibodies using the proteins, particularly ba the double-antigen-sandwich immunoassay is provided. An immunoassay kit for detecting human anti-Treponema pallidum antibodies by the method of present is also provided.
Inventors:
WANG JIAN (CN)
JIN HONGJUN (CN)
GAO CUNJI (CN)
LI ZHIJIE (CN)
ZHANG YUANMING (CN)
MU FENG (CN)
JIN HONGJUN (CN)
GAO CUNJI (CN)
LI ZHIJIE (CN)
ZHANG YUANMING (CN)
MU FENG (CN)
Application Number:
PCT/CN1999/000110
Publication Date:
August 17, 2000
Filing Date:
August 06, 1999
Export Citation:
Assignee:
WANG JIAN (CN)
JIN HONGJUN (CN)
GAO CUNJI (CN)
LI ZHIJIE (CN)
ZHANG YUANMING (CN)
MU FENG (CN)
JIN HONGJUN (CN)
GAO CUNJI (CN)
LI ZHIJIE (CN)
ZHANG YUANMING (CN)
MU FENG (CN)
International Classes:
C07K14/20; G01N33/571; A61K39/00; (IPC1-7): C07K14/20; G01N33/571; A61K39/02
Other References:
PURCELL B.K. ET AL.: "Lipid modification of the 15kD major membrane immunogen of Treponema pallidum", MOL. MICROBIOL.,, vol. 4, no. 8, 1990, pages 1371 - 1379
AKINS D.R. ET AL.: "Lipid modification of the 17kD membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity", INFECT. IMMUN.,, vol. 61, no. 4, 1993, pages 1202 - 1210
HSU P.L. ET AL.: "Sequence analysis of the 47kD major integral membrane immunogen of Treponema pallidum", INFECT. IMMUN.,, vol. 57, no. 1, 1989, pages 196 - 203
AKINS D.R. ET AL.: "Lipid modification of the 17kD membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity", INFECT. IMMUN.,, vol. 61, no. 4, 1993, pages 1202 - 1210
HSU P.L. ET AL.: "Sequence analysis of the 47kD major integral membrane immunogen of Treponema pallidum", INFECT. IMMUN.,, vol. 57, no. 1, 1989, pages 196 - 203
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (2 Fuchengmenwai Street, Beijing 7, CN)
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