Title:
MOLECULAR COMPLEX AND BIOPOLYMER ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2021/124468
Kind Code:
A1
Abstract:
Provided is a technique for mitigating the need for complicated treatments, difficulty of handling, and difficulty of exact sequencing for when repeated readings are to be performed. The present disclosure provides a molecular complex provided with single-stranded overhang double-stranded DNA, wherein the molecular complex is provided with a biopolymer, a nanopore introduction strand, a primer that is shorter than the biopolymer, a DNA polymerase that is bonded to a 3'-end part of the primer, a spacer for stopping an elongation reaction of the DNA polymerase, and a control strand, the DNA polymerase being bonded to the vicinity of the spacer (see fig. 2).
Inventors:
GOTO YUSUKE (JP)
AKAHORI RENA (JP)
FUJIOKA MICHIRU (JP)
SUMIDA NORIYUKI (JP)
AKAHORI RENA (JP)
FUJIOKA MICHIRU (JP)
SUMIDA NORIYUKI (JP)
Application Number:
PCT/JP2019/049521
Publication Date:
June 24, 2021
Filing Date:
December 18, 2019
Export Citation:
Assignee:
HITACHI HIGH TECH CORP (JP)
International Classes:
C12Q1/6869
Foreign References:
| US20180201993A1 | 2018-07-19 | |||
| JP2014534812A | 2014-12-25 | |||
| JP2018536416A | 2018-12-13 | |||
| JP2010263845A | 2010-11-25 | |||
| JP2020031557A | 2020-03-05 |
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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