Title:
MOLECULAR WEIGHT INTERNAL STANDARD AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/032342
Kind Code:
A1
Abstract:
Provided is a molecular weight internal standard, consisting of n nucleotide fragments that have different molecular weights and identifiable markers, the n nucleotide fragments comprising at least p nucleotide fragment pairs differing by one base, and one fragment of the nucleotide fragment pair differing by one base having m bases, and the other fragment having m+1 or m-1 bases; p is 1 to n/2 when n is an even number and p is 1 to (n-1)/2 when n is an odd number. Compared with current commercial molecular weight internal standards, the molecular weight internal standard can be used to identify a single base and differentiate PCR product fragments differing by one base, and is applicable to STR analysis, sequencing, large fragment analysis, and genome analysis.
Inventors:
BI WANLI (CN)
LU WEIHUA (CN)
LU WEIHUA (CN)
Application Number:
PCT/CN2016/096938
Publication Date:
March 02, 2017
Filing Date:
August 26, 2016
Export Citation:
Assignee:
NUHIGH BIOTECHNOLOGIES CO LTD (CN)
International Classes:
C12N15/11; C12Q1/68
Foreign References:
| CN105296473A | 2016-02-03 | |||
| CN101050474A | 2007-10-10 | |||
| CN104458686A | 2015-03-25 | |||
| CN102978283A | 2013-03-20 | |||
| CN103952488A | 2014-07-30 |
Other References:
YANG, PIAN ET AL.: "Designation and Preparation of Fluorescently Labelled DNA Internal Lane Standard", LIFE SCIENCE INSTRUMENTS, vol. 12, 31 December 2014 (2014-12-31), XP009508998
See also references of EP 3348642A4
See also references of EP 3348642A4
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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