Title:
MONOCLONAL ANTIBODY AGAINST HLA-G1, HLA-G4 AND HLA-G5 ISOMER MOLECULES AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/077733
Kind Code:
A1
Abstract:
Disclosed in the present invention are an antibody (YHWG-4) against HLA-G isomer molecules HLA-G1, HLA-G4 and HLA-G5 and the use thereof. The antibody (YWHG-4) is produced by hybridoma with a deposit number of CCTCC NO:2021204, and an antigen peptide (RGYYNQSEASSHTLQWMIGC) of amino acid sequences at positions 106-126 of a heavy chain of HLA-G molecules is used as an immunogen. Provided in the present invention are a nucleotide encoding the YWHG-4 antibody of the present invention and an encoding amino acid sequence thereof. Further provided in the present invention is the use of the YWHG-4 antibody in the detection of the HLA-G isomer molecules HLA-G1, HLA-G4 and HLA-G5 by means of Western blotting, immunohistochemistry, ELISA and flow cytometry.
Inventors:
YAN WEIHUA (CN)
Application Number:
PCT/CN2022/087315
Publication Date:
May 11, 2023
Filing Date:
April 18, 2022
Export Citation:
Assignee:
TAIZHOU ENZE MEDICAL CT GROUP (CN)
International Classes:
C07K16/28; C12N5/20; C12N15/13; G01N33/569; G01N33/574; G01N33/577
Foreign References:
| CN105675883A | 2016-06-15 | |||
| CN113045656A | 2021-06-29 | |||
| CN101967191A | 2011-02-09 | |||
| CN109563169A | 2019-04-02 | |||
| CN107533051A | 2018-01-02 | |||
| IN201817047794A | ||||
| US20200102390A1 | 2020-04-02 |
Other References:
LU SHENGJUN, LIANG ZHIHUI, ZHU HUIFEN, WU XIONGWEN, GONG FEILI: " Preparation of anti-HLA-G Monoclonal Antibody G11E5", CHINESE JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY, FOURTH MILITARY MEDICAL UNIVERSITY, XI'AN, CN, no. 2, 18 March 2006 (2006-03-18), CN , pages 216 - 218, XP009545264, ISSN: 1007-8738
MENIER C, ET AL: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: New tools to analyze the expression of nonclassical HLA class I molecules.", HUMAN IMMUNOLOGY, NEW YORK, NY, US, vol. 64, no. 3, 1 March 2003 (2003-03-01), US , pages 315 - 326, XP002321005, ISSN: 0198-8859, DOI: 10.1016/S0198-8859(02)00821-2
FURUKAWA ATSUSHI, MEGURO MANAMI, YAMAZAKI RIKA, WATANABE HIROSHI, TAKAHASHI AMI, KUROKI KIMIKO, MAENAKA KATSUMI: "Evaluation of the Reactivity and Receptor Competition of HLA-G Isoforms toward Available Antibodies: Implications of Structural Characteristics of HLA-G Isoforms", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 23, pages 5947, XP055946079, DOI: 10.3390/ijms20235947
LOUSTAU, M. ET AL.: "HLA-G Neo-Expression on Tumors", FRONTIERS IN IMMUNOLOGY, vol. 11, 14 August 2020 (2020-08-14), XP055746809, DOI: 10.3389/fimmu.2020.01685
MENIER C, ET AL: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: New tools to analyze the expression of nonclassical HLA class I molecules.", HUMAN IMMUNOLOGY, NEW YORK, NY, US, vol. 64, no. 3, 1 March 2003 (2003-03-01), US , pages 315 - 326, XP002321005, ISSN: 0198-8859, DOI: 10.1016/S0198-8859(02)00821-2
FURUKAWA ATSUSHI, MEGURO MANAMI, YAMAZAKI RIKA, WATANABE HIROSHI, TAKAHASHI AMI, KUROKI KIMIKO, MAENAKA KATSUMI: "Evaluation of the Reactivity and Receptor Competition of HLA-G Isoforms toward Available Antibodies: Implications of Structural Characteristics of HLA-G Isoforms", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 23, pages 5947, XP055946079, DOI: 10.3390/ijms20235947
LOUSTAU, M. ET AL.: "HLA-G Neo-Expression on Tumors", FRONTIERS IN IMMUNOLOGY, vol. 11, 14 August 2020 (2020-08-14), XP055746809, DOI: 10.3389/fimmu.2020.01685
Attorney, Agent or Firm:
ZHEJIANG HANGZHOU JINTONG PATENT AGENCY CO., LTD. (CN)
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