Title:
MONOCLONAL ANTIBODY SPECIFICALLY RECOGNIZING ADENO-ASSOCIATED VIRUS CAP PROTEIN
Document Type and Number:
WIPO Patent Application WO/1996/029349
Kind Code:
A1
Abstract:
A monoclonal antibody specifically recognizing the adeno-associated virus CAP protein and produced by hybridomas which have been formed through fusion between lymphocytes prepared from a mammal immunized with the adeno-associated virus CAP protein or a recombinant of the protein as an antigen and a myeloma cell line. Because of being a novel antibody and capable of specifically recognizing the adeno-associated virus CAP protein, the antibody is applicable to the detection of the adeno-associated virus and the purification of an adeno-associated virus vector for gene therapy.
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Inventors:
SHIMADA TAKASHI (JP)
KUMA HIDEKAZU (JP)
SUZUKI YOUSUKE (JP)
KUMA HIDEKAZU (JP)
SUZUKI YOUSUKE (JP)
Application Number:
PCT/JP1996/000655
Publication Date:
September 26, 1996
Filing Date:
March 15, 1996
Export Citation:
Assignee:
HISAMITSU PHARMACEUTICAL CO (JP)
SHIMADA TAKASHI (JP)
KUMA HIDEKAZU (JP)
SUZUKI YOUSUKE (JP)
SHIMADA TAKASHI (JP)
KUMA HIDEKAZU (JP)
SUZUKI YOUSUKE (JP)
International Classes:
C07K16/08; C12N5/20; C12P21/08; G01N33/569; G01N33/68; (IPC1-7): C07K16/08; C12N5/20; C12N15/06; C12N15/64; C12N15/86; C12P21/08; G01N33/569; G01N33/577
Other References:
J. VIROL., 1988, Vol. 62, No. 9, TREMPE J.P. et al., "Alternate mRNA Splicing is Required for Synthesis of Adeno-Associated Virus VP1 Capsid Protein", p. 3356-3363.
VIROLOGY, (1988), Vol. 167, No. 1, CASSINOTTI P. et al., "Organization of the Adeno-Associated Virus (AAV) Capsid Gene: Mapping of a Minor Spliced mRNA Coding for Virus Capsid Protein 1", p. 176-184.
J. VIROL., 1992, Vol. 66, No. 1, HUNTER L.A. et al., "Colocalization of Adeno-associated Virus Rep and Capsid Proteins in the Nuclei of Infected Cells", p. 317-324.
J. VIROL., (9. 1995), Vol. 69, No. 9, WISTUBA A. et al., "Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins", p. 5311-5319.
See also references of EP 0819701A4
VIROLOGY, (1988), Vol. 167, No. 1, CASSINOTTI P. et al., "Organization of the Adeno-Associated Virus (AAV) Capsid Gene: Mapping of a Minor Spliced mRNA Coding for Virus Capsid Protein 1", p. 176-184.
J. VIROL., 1992, Vol. 66, No. 1, HUNTER L.A. et al., "Colocalization of Adeno-associated Virus Rep and Capsid Proteins in the Nuclei of Infected Cells", p. 317-324.
J. VIROL., (9. 1995), Vol. 69, No. 9, WISTUBA A. et al., "Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins", p. 5311-5319.
See also references of EP 0819701A4
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