Title:
MONOCYTE ADHESION
Document Type and Number:
WIPO Patent Application WO2003048783
Kind Code:
A3
Abstract:
The invention relates to a method of assaying leukocyte binding to solid phase heat shock protein (HSP) which comprises contacting HSP on a solid support with a suspension in a suitable medium of monocytes or a cell line having adhesion properties similar to monocytes and quantitating the number of cells bound to the support. The invention also relates to a method of assaying leukocyte binding via the CD14/TLR signalling complex to a target ligand which comprises contacting the ligand on a solid phase with a suspension in a suitable medium of monocytes or a cell line having adhesion properties similar to monocytes or a cell line transfected with complex components and quantitating the number of cells bound to the solid phase. Agents identified as inhibitors in these assays may be useful in the treatment or prevention of atherosclerosis or related clinical disease and/or for the treatment of conditions where stressed cells in a diseased tissue are targeted by chronic inflammatory responses.
Inventors:
POSTON ROBIN (GB)
Application Number:
PCT/GB2002/005223
Publication Date:
February 26, 2004
Filing Date:
November 21, 2002
Export Citation:
Assignee:
POSTON ROBIN (GB)
International Classes:
G01N33/569; (IPC1-7): G01N33/569; G01N33/50; G01N33/566
Domestic Patent References:
| WO1994029720A1 | 1994-12-22 | |||
| WO2001065257A2 | 2001-09-07 |
Other References:
KOL A ET AL: "Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells.", JOURNAL OF IMMUNOLOGY (BALTIMORE, MD.: 1950) UNITED STATES 1 JAN 2000, vol. 164, no. 1, 1 January 2000 (2000-01-01), pages 13 - 17, XP002262385, ISSN: 0022-1767
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