Title:
MOTILE SPERM SEPARATION METHOD AND SEPARATION DEVICE, AND SPERM LIQUID FOR INSEMINATION
Document Type and Number:
WIPO Patent Application WO/2016/035799
Kind Code:
A1
Abstract:
Provided is a motile sperm separation method capable of separating motile sperm with high efficiency. The motile sperm separation method, in which a narrow buffer solution channel and an upstream sperm path are connected in a preselection sperm storage tank, thereby allowing highly motile sperm to be selected through rheotaxis via the upstream sperm path, is configured such that an environment is created in preparation for a high-density sperm concentration area to form near a connecting base end of the upstream sperm path and for rheotaxis to occur. The motile sperm separation method is further characterized in that, in order to form the high-density sperm concentration area, the buffer solution channel is branched from the base end so as to be bifurcated, and the respective tip ends of both bifurcated channels of the buffer solution channel are gradually tapered.
More Like This:
Inventors:
YAMASHITA KENICHI (JP)
MIYAZAKI MASAYA (JP)
NAGATA MARIA PORTIA (JP)
MAEDA HIDEAKI (JP)
MIYAZAKI MASAYA (JP)
NAGATA MARIA PORTIA (JP)
MAEDA HIDEAKI (JP)
Application Number:
PCT/JP2015/074895
Publication Date:
March 10, 2016
Filing Date:
September 01, 2015
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
International Classes:
C12N1/02; C12M1/26; C12N5/076
Domestic Patent References:
| WO2014136464A1 | 2014-09-12 |
Other References:
KEN'ICHI YAMASHITA: "High-throughput sorting of healthy and motile bovine sperm with coexisting sex selection technology for effective artificial insemination and improved animal reproduction", JST HATSU SHIN GIJUTSU SETSUMEIKAI TOJITSU HAIFU SHIRYO, 7 March 2014 (2014-03-07), Retrieved from the Internet [retrieved on 20151218]
SEO, D. ET AL.: "Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure", MICROFLUIDICS AND NANOFLUIDICS, vol. 3, 2007, pages 561 - 570, XP019522724, doi:10.1007/s10404-006-0142-3
KEN'ICHI YAMASHITA ET AL.: "Micro Ryutai Sosa ni yori Seishi no Undo o Yudo shi, Genki na Seishi Dake o Hoshu suru Gijutsu", SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS DAI 30 KAI KENKYUKAI KOEN YOSHISHU, 2 October 2014 (2014-10-02), pages 62
SEO, D. ET AL.: "Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure", MICROFLUIDICS AND NANOFLUIDICS, vol. 3, 2007, pages 561 - 570, XP019522724, doi:10.1007/s10404-006-0142-3
KEN'ICHI YAMASHITA ET AL.: "Micro Ryutai Sosa ni yori Seishi no Undo o Yudo shi, Genki na Seishi Dake o Hoshu suru Gijutsu", SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS DAI 30 KAI KENKYUKAI KOEN YOSHISHU, 2 October 2014 (2014-10-02), pages 62
Attorney, Agent or Firm:
MATSUO KENICHIRO (JP)
Ken-ichiro Matsuo (JP)
Ken-ichiro Matsuo (JP)
Download PDF: