Title:
MREC MUTANT AND USE THEREOF IN L-VALINE FERMENTATIVE PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2023/246071
Kind Code:
A1
Abstract:
The present invention provides an mreC mutant and use thereof in L-valine fermentative production. The mreC mutant is a protein obtained by mutating proline at position 150 in the wild-type mreC amino acid sequence into leucine. The introduction of an mreC point mutation into the microbial genome, i.e., a C-to-T mutation at position 449 of the coding region in the wild-type mreC gene nucleotide sequence, can significantly improve the production capacity and biomass of an engineering strain of a high valine production. The knock-out and introduction of related enzyme genes of the L-valine anaerobic fermentation pathway further provide proper carbon metabolic flows and reducing capacity balance control in the L-valine production process. By the coordinative regulation of multiple genes, a metabolic pathway suitable for L-valine fermentative production is formed, thus achieving a high-level synthesis of L-valine in microorganisms.
Inventors:
ZHANG XUELI (CN)
GUO HENGHUA (CN)
LIU PINGPING (CN)
ZHANG DONGZU (CN)
TANG JINLEI (CN)
LIU SHUPENG (CN)
GUO HENGHUA (CN)
LIU PINGPING (CN)
ZHANG DONGZU (CN)
TANG JINLEI (CN)
LIU SHUPENG (CN)
Application Number:
PCT/CN2022/143630
Publication Date:
December 28, 2023
Filing Date:
December 29, 2022
Export Citation:
Assignee:
ANHUI HUAHENG BIOTECHNOLOGY CO LTD (CN)
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
HEFEI HUAHENG BIOENGINEERING CO LTD (CN)
BAYANNUR HUAHENG BIOTECHNOLOGY CO LTD (CN)
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
HEFEI HUAHENG BIOENGINEERING CO LTD (CN)
BAYANNUR HUAHENG BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07K14/245; C12N1/21; C12N15/31; C12P13/08
Foreign References:
| CN104974946A | 2015-10-14 | |||
| CN114349831A | 2022-04-15 | |||
| CN106520655A | 2017-03-22 | |||
| CN110607268A | 2019-12-24 | |||
| CN104561074A | 2015-04-29 |
Other References:
LEAVER MARK, ERRINGTON JEFF: "Roles for MreC and MreD proteins in helical growth of the cylindrical cell wall in Bacillus subtilis", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 57, no. 5, 1 September 2005 (2005-09-01), GB , pages 1196 - 1209, XP093120866, ISSN: 0950-382X, DOI: 10.1111/j.1365-2958.2005.04736.x
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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