Title:
MRNA CAPPING ENZYME AND METHODS OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/154716
Kind Code:
A3
Abstract:
Provided herein is a method for capping RNA in vitro. In some embodiments the capping reaction may be done using an RNA guanylyltransferase capping enzyme from a Tupanviruses virus, and in particular a capping enzyme from Tupanvirus soda lake virus or Tupanvirus deep ocean virus, or a fragment or variant thereof. Compositions and kits for capping RNA in vitro are also provided. Also included are methods, compositions and kits for the manufacturer and capping of therapeutic RNA oligonucleotides, such as RNA-based vaccines and therapeutics.
Inventors:
STRIEKER MATTHIAS (US)
HUMBERT MICHAEL (US)
KOGLIN ALEXANDER (US)
ELIASON GRANT (US)
HUMBERT MICHAEL (US)
KOGLIN ALEXANDER (US)
ELIASON GRANT (US)
Application Number:
PCT/US2023/062161
Publication Date:
November 09, 2023
Filing Date:
February 07, 2023
Export Citation:
Assignee:
NATURES TOOLBOX INC (US)
International Classes:
C07H21/04; C07H19/20; C07H21/02; C12N15/10; C12P19/34; C12Q1/6816
Domestic Patent References:
| WO2021212034A1 | 2021-10-21 |
Foreign References:
| US20210054016A1 | 2021-02-25 |
Other References:
BELL PHILIP J.L.: "Evidence supporting a viral origin of the eukaryotic nucleus", VIRUS RESEARCH, AMSTERDAM, NL, vol. 289, 20 September 2020 (2020-09-20), NL , XP086289078, ISSN: 0168-1702, DOI: 10.1016/j.virusres.2020.198168
DATABASE PROTEIN ANONYMOUS : "mRNA-capping enzyme [Saudi moumouvirus]", XP093109228, retrieved from NCBI
DATABASE PROTEIN ANONYMOUS : "mRNA-capping enzyme [Saudi moumouvirus]", XP093109228, retrieved from NCBI
Attorney, Agent or Firm:
KERR, David, S. (US)
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