Title:
MRNA CONSTRUCT FOR PROTEIN EXPRESSION, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/107647
Kind Code:
A1
Abstract:
The present invention relates to an mRNA or mRNA construct including: (a) a region encoding a protein or peptide of interest; (b) a β-globin UTR region at the 5'end; and (c) a region at the 3'end in which BGH, BGH-IRES, or IRES-BGH is inserted. An mRNA or mRNA construct according to the present invention improves intracellular expression or expression stability, and intracellular persistence of a chimeric antigen receptor or chimeric antigen receptor protein (CAR protein). In addition, the mRNA or mRNA construct increases the in vivo safety of the chimeric antigen receptor, and increases the ability thereof to kill cancer cells, and thus can be effectively used in anticancer pharmaceutical compositions and anticancer immunotherapy targeting solid tumors.
Inventors:
KIM TAE DON (KR)
KANG HEE YOUNG (KR)
CHOI IN PYO (KR)
KANG HEE YOUNG (KR)
CHOI IN PYO (KR)
Application Number:
PCT/KR2020/016988
Publication Date:
June 03, 2021
Filing Date:
November 26, 2020
Export Citation:
Assignee:
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
C12N15/85; A61K35/17; A61P35/00; C07K14/705; C07K14/725; C12N5/0783
Domestic Patent References:
| WO2016197121A1 | 2016-12-08 |
Foreign References:
| US20070298417A1 | 2007-12-27 | |||
| US5807707A | 1998-09-15 | |||
| KR20190152938A | 2019-11-26 |
Other References:
YUAN HU, ET AL.: "Chimeric antigen receptor (CAR)-transduced natural killer cells in tumor immunotherapy", ACTA PHARMACOLOGICA SINICA, vol. 39, no. 2, 1 February 2018 (2018-02-01), GB, pages 167 - 176, XP055625054, ISSN: 1671-4083, DOI: 10.1038/aps.2017.125
KAKOKI, MASAO ET AL.: "Altering the Expression in Mice of Genes by Modifying Their 3 Regions", DEVELOPMENTAL CELL, vol. 6, 1 April 2004 (2004-04-01), pages 597 - 606, XP055137714, DOI: 10.1016/S1534-5807(04)00094-2
RODRIGO VAZQUEZ-LOMBARDI ET AL.: "Transient expression of human antibodies in mammalian cells", NAT PROTOC., vol. 13, no. 1, January 2018 (2018-01-01), pages 99 - 117
"Remington s Pharmaceutical Sciences", 1995
See also references of EP 4067495A4
KAKOKI, MASAO ET AL.: "Altering the Expression in Mice of Genes by Modifying Their 3 Regions", DEVELOPMENTAL CELL, vol. 6, 1 April 2004 (2004-04-01), pages 597 - 606, XP055137714, DOI: 10.1016/S1534-5807(04)00094-2
RODRIGO VAZQUEZ-LOMBARDI ET AL.: "Transient expression of human antibodies in mammalian cells", NAT PROTOC., vol. 13, no. 1, January 2018 (2018-01-01), pages 99 - 117
"Remington s Pharmaceutical Sciences", 1995
See also references of EP 4067495A4
Attorney, Agent or Firm:
B&IP-JOOWON PATENT AND LAW FIRM (KR)
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