Title:
MULTICOMPONENT DEGRADABLE CATIONIC POLYMERS
Document Type and Number:
WIPO Patent Application WO/2010/132879
Kind Code:
A3
Abstract:
Degradable polymers were synthesized that self-assemble with DNA to form particles that are effective for gene delivery. Small changes to polymer synthesis conditions, particle formulation conditions, and polymer structure provides significant changes to efficacy in a cell-type dependent manner. Polymers presented here are more effective than commercially available materials, such as LIPOFECTAMINE 2000™, FUGENE®, or polyethylenimine (PEI), for gene delivery to cancerous fibroblasts or human primary fibroblasts. The presently disclosed materials may be useful for cancer therapeutics and regenerative medicine.
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Inventors:
GREEN JORDAN J (US)
SUNSHINE JOEL C (US)
BHISE NUPURA S (US)
SHMUELI RON B (US)
TZENG STEPHANY Y (US)
SUNSHINE JOEL C (US)
BHISE NUPURA S (US)
SHMUELI RON B (US)
TZENG STEPHANY Y (US)
Application Number:
PCT/US2010/035127
Publication Date:
March 31, 2011
Filing Date:
May 17, 2010
Export Citation:
Assignee:
UNIV JOHNS HOPKINS (US)
GREEN JORDAN J (US)
SUNSHINE JOEL C (US)
BHISE NUPURA S (US)
SHMUELI RON B (US)
TZENG STEPHANY Y (US)
GREEN JORDAN J (US)
SUNSHINE JOEL C (US)
BHISE NUPURA S (US)
SHMUELI RON B (US)
TZENG STEPHANY Y (US)
International Classes:
C07C225/04; A61K31/7105; A61K47/34; A61P35/00; C08G61/12
Other References:
SUNSHINE, J. ET AL.: "Small-Molecule End-Groups of Linear Polymer Determine Cell-type Gene-Delivery Efficacy", ADVANCED MATERIALS, vol. 21, no. 48, December 2009 (2009-12-01), WEINHEIM, GERMANY, pages 4947 - 4951, XP055095832, DOI: doi:10.1002/adma.200901718
LEE, J.-S. ET AL.: "Gold, Poly(beta-amino ester) Nanoparticles for Small Interfering RNA Delivery", NANO LETTERS, vol. 9, no. 6, 7 May 2009 (2009-05-07), pages 2402 - 2406, XP002629297, DOI: doi:10.1021/nl9009793
GREEN, J. J. ET AL.: "Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus", ADVANCED MATERIALS, vol. 19, no. 19, 2007, WEINHEIM, GERMANY, pages 2836 - 2842
ZUGATES, G. T. ET AL.: "Gene Delivery Properties of End-Modified Poly(beta- amino ester)", BIOCONJUGATE CHEMISTRY, vol. 18, no. 6, 2007, pages 1887 - 1896, XP055119752, DOI: doi:10.1021/bc7002082
LEE, J.-S. ET AL.: "Gold, Poly(beta-amino ester) Nanoparticles for Small Interfering RNA Delivery", NANO LETTERS, vol. 9, no. 6, 7 May 2009 (2009-05-07), pages 2402 - 2406, XP002629297, DOI: doi:10.1021/nl9009793
GREEN, J. J. ET AL.: "Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus", ADVANCED MATERIALS, vol. 19, no. 19, 2007, WEINHEIM, GERMANY, pages 2836 - 2842
ZUGATES, G. T. ET AL.: "Gene Delivery Properties of End-Modified Poly(beta- amino ester)", BIOCONJUGATE CHEMISTRY, vol. 18, no. 6, 2007, pages 1887 - 1896, XP055119752, DOI: doi:10.1021/bc7002082
Attorney, Agent or Firm:
CHILDERS, Jeffrey, W. (5th FloorBaltimore, Maryland, US)
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