Title:
MULTIPLE-GENE KNOCKOUT MICROORGANISM BELONGING TO GENUS ASPERGILLUS AND PRODUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/095864
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a transformed microorganism belonging to the genus Aspergillus deficient of at least two selectable marker genes usable in a marker recycling method; and a composition for use of the transformed microorganism. The purpose is achieved by a transformed microorganism belonging to the genus Aspergillus deficient of at least two selectable marker genes usable in a marker recycling method on the chromosome thereof; and a composition that is for transforming a microorganism belonging to the genus Aspergillus and that comprises at least two nucleic acid fragments including a loop-out region and a selectable marker gene usable in a marker recycling method between homologous recombination regions, wherein the selectable marker genes include, for example, a tryptophan biosynthesis gene and a gene different from the tryptophan biosynthesis gene.
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Inventors:
HARA SEIICHI (JP)
Application Number:
PCT/JP2019/043173
Publication Date:
May 14, 2020
Filing Date:
November 04, 2019
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N1/15; C12N15/31; C12N15/52; C12N15/65; C12N15/79
Domestic Patent References:
| WO2017135317A1 | 2017-08-10 |
Foreign References:
| JP2006158269A | 2006-06-22 | |||
| JP2007222055A | 2007-09-06 | |||
| JPS6261039B2 | 1987-12-18 | |||
| JPS574609B2 | 1982-01-27 | |||
| JP2018208022A | 2018-11-05 | |||
| JP2019109602A | 2019-07-04 |
Other References:
TANI, S. ET AL.: "Reversible impairment of the ku80 gene by a recyclable 1-7 marker in Aspergillus aculeatus", AME EXPRESS, vol. 3, 2013, pages 4, XP055705054, Retrieved from the Internet
GOOSEN, T. ET AL.: "Tryptophan auxotrophic mutants in Aspergillus niger: 1-7 Inactivation of the trpC gene by cotransformation mutagenesis", MOLECULAR AND GENERAL GENETICS, vol. 219, 1989, pages 282 - 288, XP055705057
TOYN, J. H. ET AL.: "A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance", YEAST, vol. 16, 2000, pages 553 - 560, XP055705060
NIELSEN, M. L. ET AL.: "Efficient PCR-based gene targeting with a recyclable marker for Aspergillus nidulans", FUNGAL GENETICS AND BIOLOGY, vol. 43, no. 1, 2006, pages 54 - 64, XP024918908, DOI: 10.1016/j.fgb.2005.09.005
TAKAHASHI, T. ET AL.: "Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae", MOLECULAR GENETICS AND GENOMICS, vol. 275, no. 5, 2006, pages 460 - 470, XP019428009, DOI: 10.1007/s00438-006-0104-1
TANI ET AL., AMB EXPRESS, vol. 3, 2013, pages 4, Retrieved from the Internet
GOOSEN ET AL., MOLECULAR AND GENERAL GENETICS, vol. 219, 1989, pages 282 - 288
TOYN ET AL., YEAST, vol. 16, 2000, pages 553 - 560
MOL. GEN. GENET., vol. 218, 1989, pages 99 - 104
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
LIPMAN-PEARSON, SCIENCE, vol. 227, 1985, pages 1435 - 1441
MOL. GENET. GENOMICS, vol. 275, 2006, pages 460
BIOSCI. BIOTECHNOL. BIOCHEM, vol. 70, 2006, pages 135
BMC MICROBIOL, vol. 14, 2014, pages 90, Retrieved from the Internet
See also references of EP 3878944A4
GOOSEN, T. ET AL.: "Tryptophan auxotrophic mutants in Aspergillus niger: 1-7 Inactivation of the trpC gene by cotransformation mutagenesis", MOLECULAR AND GENERAL GENETICS, vol. 219, 1989, pages 282 - 288, XP055705057
TOYN, J. H. ET AL.: "A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance", YEAST, vol. 16, 2000, pages 553 - 560, XP055705060
NIELSEN, M. L. ET AL.: "Efficient PCR-based gene targeting with a recyclable marker for Aspergillus nidulans", FUNGAL GENETICS AND BIOLOGY, vol. 43, no. 1, 2006, pages 54 - 64, XP024918908, DOI: 10.1016/j.fgb.2005.09.005
TAKAHASHI, T. ET AL.: "Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae", MOLECULAR GENETICS AND GENOMICS, vol. 275, no. 5, 2006, pages 460 - 470, XP019428009, DOI: 10.1007/s00438-006-0104-1
TANI ET AL., AMB EXPRESS, vol. 3, 2013, pages 4, Retrieved from the Internet
GOOSEN ET AL., MOLECULAR AND GENERAL GENETICS, vol. 219, 1989, pages 282 - 288
TOYN ET AL., YEAST, vol. 16, 2000, pages 553 - 560
MOL. GEN. GENET., vol. 218, 1989, pages 99 - 104
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
LIPMAN-PEARSON, SCIENCE, vol. 227, 1985, pages 1435 - 1441
MOL. GENET. GENOMICS, vol. 275, 2006, pages 460
BIOSCI. BIOTECHNOL. BIOCHEM, vol. 70, 2006, pages 135
BMC MICROBIOL, vol. 14, 2014, pages 90, Retrieved from the Internet
See also references of EP 3878944A4
Attorney, Agent or Firm:
MORIMOTO, Toshiaki (JP)
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