Title:
MULTIPLE PROTEASES DEFICIENT FILAMENTOUS FUNGAL CELLS AND METHODS OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2015/004241
Kind Code:
A3
Abstract:
The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.
Inventors:
LANDOWSKI CHRISTOPHER (FI)
HUUSKONEN ANNE (FI)
WESTERHOLM-PARVINEN ANN (FI)
SALOHEIMO MARKKU (FI)
KANERVA ANNE (FI)
HILTUNEN JUKKA (FI)
HUUSKONEN ANNE (FI)
WESTERHOLM-PARVINEN ANN (FI)
SALOHEIMO MARKKU (FI)
KANERVA ANNE (FI)
HILTUNEN JUKKA (FI)
Application Number:
PCT/EP2014/064820
Publication Date:
March 19, 2015
Filing Date:
July 10, 2014
Export Citation:
Assignee:
NOVARTIS AG (CH)
GLYKOS FINLAND OY (FI)
GLYKOS FINLAND OY (FI)
International Classes:
C12N1/21; C12N9/14; C12N9/58; C12N15/80; C12P21/02
Domestic Patent References:
| WO2006110677A2 | 2006-10-19 | |||
| WO2011075677A2 | 2011-06-23 | |||
| WO2009126463A2 | 2009-10-15 |
Other References:
HARMAN G E, KUBICEK C P (EDS.): "Trichoderma and Gliocladium", vol. 2, 1998, TAYLOR & FRANCIS LTD, GB, LONDON, ISBN: 978-0-7484-0805-4, article MÄNTYLÄ A, PALOHEIMO M, SUOMINEN P: "Industrial Mutants and Recombinant Strains of Trichoderma reesei", pages: 291 - 309, XP008134641
JANAS P.: "Production of extracellular enzymes by low-protease mutants of Trichoderma reesei", TECHNOLOGIA ALIMENTARIA, vol. 2, no. 2, 2003, pages 103 - 114, XP002629472
JAEWOO YOON ET AL: "Construction of quintuple protease gene disruptant for heterologous protein production in Aspergillus oryzae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 82, no. 4, 24 December 2008 (2008-12-24), pages 691 - 701, XP019705459, ISSN: 1432-0614
OWEN P. WARD: "Production of recombinant proteins by filamentous fungi", BIOTECHNOLOGY ADVANCES, vol. 30, no. 5, 1 September 2012 (2012-09-01), pages 1119 - 1139, XP055088348, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2011.09.012
VAN DEN HOMBERGH J P ET AL: "Aspergillus as a host for heterologous protein production: the problem of proteases", TRENDS IN BIOTECHNOLOGY, vol. 15, no. 7, 1 July 1997 (1997-07-01), pages 256 - 263, XP027556962, ISSN: 0167-7799, [retrieved on 19970701]
IRINA S. DRUZHININA ET AL: "Novel traits of Trichoderma predicted through the analysis of its secretome", FEMS MICROBIOLOGY LETTERS, vol. 337, no. 1, 18 December 2012 (2012-12-18), pages 1 - 9, XP055088413, ISSN: 0378-1097, DOI: 10.1111/j.1574-6968.2012.02665.x
BELÉN SUÁREZ M ET AL: "Characterization of genes encoding novel peptidases in the biocontrol fungus Trichoderma harzianum CECT 2413 using the TrichoEST functional genomics approach", CURRENT GENETICS, vol. 51, no. 5, 6 April 2007 (2007-04-06), pages 331 - 342, XP019515006, ISSN: 1432-0983, DOI: 10.1007/S00294-007-0130-5
DIEGO MARTINEZ ET AL: "Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)", NATURE BIOTECHNOLOGY, vol. 26, no. 5, 1 May 2008 (2008-05-01), pages 553 - 560, XP002689190, ISSN: 1087-0156, [retrieved on 20080504], DOI: 10.1038/NBT1403
CHRISTIAN P KUBICEK ET AL: "Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma", GENOME BIOLOGY, vol. 12, no. 4, 18 April 2011 (2011-04-18), pages R40, XP021102327, ISSN: 1465-6906, DOI: 10.1186/GB-2011-12-4-R40
DATABASE UniProt [online] 19 October 2011 (2011-10-19), "RecName: Full=Leukotriene A(4) hydrolase; Short=LTA-4 hydrolase; EC=3.3.2.6;", XP002730645, retrieved from EBI accession no. UNIPROT:G0RM29 Database accession no. G0RM29
JUNICHIRO MARUI ET AL: "Comparison of expression and enzymatic properties of lysine aminopeptidases ApsA and ApsB", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, vol. 28, no. 8, 11 May 2012 (2012-05-11), pages 2643 - 2650, XP035080901, ISSN: 1573-0972, DOI: 10.1007/S11274-012-1074-6
JANAS P.: "Production of extracellular enzymes by low-protease mutants of Trichoderma reesei", TECHNOLOGIA ALIMENTARIA, vol. 2, no. 2, 2003, pages 103 - 114, XP002629472
JAEWOO YOON ET AL: "Construction of quintuple protease gene disruptant for heterologous protein production in Aspergillus oryzae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 82, no. 4, 24 December 2008 (2008-12-24), pages 691 - 701, XP019705459, ISSN: 1432-0614
OWEN P. WARD: "Production of recombinant proteins by filamentous fungi", BIOTECHNOLOGY ADVANCES, vol. 30, no. 5, 1 September 2012 (2012-09-01), pages 1119 - 1139, XP055088348, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2011.09.012
VAN DEN HOMBERGH J P ET AL: "Aspergillus as a host for heterologous protein production: the problem of proteases", TRENDS IN BIOTECHNOLOGY, vol. 15, no. 7, 1 July 1997 (1997-07-01), pages 256 - 263, XP027556962, ISSN: 0167-7799, [retrieved on 19970701]
IRINA S. DRUZHININA ET AL: "Novel traits of Trichoderma predicted through the analysis of its secretome", FEMS MICROBIOLOGY LETTERS, vol. 337, no. 1, 18 December 2012 (2012-12-18), pages 1 - 9, XP055088413, ISSN: 0378-1097, DOI: 10.1111/j.1574-6968.2012.02665.x
BELÉN SUÁREZ M ET AL: "Characterization of genes encoding novel peptidases in the biocontrol fungus Trichoderma harzianum CECT 2413 using the TrichoEST functional genomics approach", CURRENT GENETICS, vol. 51, no. 5, 6 April 2007 (2007-04-06), pages 331 - 342, XP019515006, ISSN: 1432-0983, DOI: 10.1007/S00294-007-0130-5
DIEGO MARTINEZ ET AL: "Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)", NATURE BIOTECHNOLOGY, vol. 26, no. 5, 1 May 2008 (2008-05-01), pages 553 - 560, XP002689190, ISSN: 1087-0156, [retrieved on 20080504], DOI: 10.1038/NBT1403
CHRISTIAN P KUBICEK ET AL: "Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma", GENOME BIOLOGY, vol. 12, no. 4, 18 April 2011 (2011-04-18), pages R40, XP021102327, ISSN: 1465-6906, DOI: 10.1186/GB-2011-12-4-R40
DATABASE UniProt [online] 19 October 2011 (2011-10-19), "RecName: Full=Leukotriene A(4) hydrolase; Short=LTA-4 hydrolase; EC=3.3.2.6;", XP002730645, retrieved from EBI accession no. UNIPROT:G0RM29 Database accession no. G0RM29
JUNICHIRO MARUI ET AL: "Comparison of expression and enzymatic properties of lysine aminopeptidases ApsA and ApsB", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, vol. 28, no. 8, 11 May 2012 (2012-05-11), pages 2643 - 2650, XP035080901, ISSN: 1573-0972, DOI: 10.1007/S11274-012-1074-6
Attorney, Agent or Firm:
CABINET PLASSERAUD (Lyon, FR)
Download PDF: