Title:
MUTANT D-AMINOTRANSFERASE AND PROCESS FOR PRODUCING OPTICALLY ACTIVE GLUTAMIC ACID DERIVATIVE USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2004/053125
Kind Code:
A1
Abstract:
By substituting an amino acid residue at least at one position participating in the efficient formation of (2R,4R)-monatin (100-position, 180- to 183-positions, 243-position and 244-position) in the amino acid sequence of wild type D-aminotransferase represented by SEQ ID NO:2 in Sequence Listing, the D-aminotransferase can be mutated so that it can efficiently produce (2R,4R)-monatin having an elevated sweetness from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid.
Inventors:
SUGIYAMA MASAKAZU (JP)
WATANABE KUNIHIKO (JP)
KASHIWAGI TATSUKI (JP)
SUZUKI EI-ICHIRO (JP)
WATANABE KUNIHIKO (JP)
KASHIWAGI TATSUKI (JP)
SUZUKI EI-ICHIRO (JP)
Application Number:
PCT/JP2003/015714
Publication Date:
June 24, 2004
Filing Date:
December 09, 2003
Export Citation:
Assignee:
AJINOMOTO KK (JP)
SUGIYAMA MASAKAZU (JP)
WATANABE KUNIHIKO (JP)
KASHIWAGI TATSUKI (JP)
SUZUKI EI-ICHIRO (JP)
SUGIYAMA MASAKAZU (JP)
WATANABE KUNIHIKO (JP)
KASHIWAGI TATSUKI (JP)
SUZUKI EI-ICHIRO (JP)
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12N9/10; C12P17/10; (IPC1-7): C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12P13/04
Domestic Patent References:
| WO2003056026A1 | 2003-07-10 |
Foreign References:
| US6337190B1 | 2002-01-08 |
Other References:
SUGIO S, ET AL: "CRYSTAL STRUCTURE OF A D-AMINO ACID AMINOTRANSFERASE: HOW THE PROTEIN CONTROLS STEREOSELECTIVITY", BIOCHEMISTRY, vol. 34, no. 30, 1 August 1995 (1995-08-01), pages 9661 - 9669, XP002977413
RO H S, ET AL: "SITE-DIRECTED MUTAGENESIS OF THE AMINO ACID RESIDUES IN BETA-STRAND III (VAL30-VAL36) OF D-AMINO ACID AMINOTRANSFERASE OF BACILLUS SP. YM-1", FEBS LETTERS, vol. 398, no. 2-3, 2 December 1996 (1996-12-02), pages 141 - 145, XP002977414
KISHIMOTO K, ET AL: "ROLE OF LEUCINE 201 OF THERMOSTABLE D-AMINO ACID AMINOTRANSFERASE FROM A THERMOPHILE, BACILLUS SP. YM-1", J BIOCHEM, vol. 117, no. 4, April 1995 (1995-04-01), pages 691 - 696, XP002977415
RO H S, ET AL: "SITE-DIRECTED MUTAGENESIS OF THE AMINO ACID RESIDUES IN BETA-STRAND III (VAL30-VAL36) OF D-AMINO ACID AMINOTRANSFERASE OF BACILLUS SP. YM-1", FEBS LETTERS, vol. 398, no. 2-3, 2 December 1996 (1996-12-02), pages 141 - 145, XP002977414
KISHIMOTO K, ET AL: "ROLE OF LEUCINE 201 OF THERMOSTABLE D-AMINO ACID AMINOTRANSFERASE FROM A THERMOPHILE, BACILLUS SP. YM-1", J BIOCHEM, vol. 117, no. 4, April 1995 (1995-04-01), pages 691 - 696, XP002977415
Attorney, Agent or Firm:
Sakai, Hiroaki (Kasumigaseki Building 2-5, Kasumigaseki 3-chom, Chiyoda-ku Tokyo, JP)
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