Title:
MUTANT AND GENE ENCODING THE SAME
Document Type and Number:
WIPO Patent Application WO/2007/142243
Kind Code:
A1
Abstract:
A CGTase mutant derived from parent γ-CGTase by substituting at least one of the amino acid residues by an amino acid residue differing from the corresponding amino acid residue of the parent γ-CGTase, which has an improved specific activity relating to the synthesis of γ-cyclodextrin compared with the parent γ-CGTase; a gene encoding the amino acid sequence of this mutant; and a method of producing the above-described CGTase mutant which comprises culturing a transformant, which has been transformed by a recombinant vector having the above gene or by chromosomal homologous recombination, and collecting the cyclodextrin glucanotransferase mutant thus produced by the above-described transformant. Namely, it is intended to provide an improved CGTase mutant, which has a higher specific activity than the parent γ-CGTase and enables the reduction of the amount of oxygen required for obtaining a desired CD composition, by mutating γ-cyclodextrin glucanotransferase (γ-CGTase); a gene of the CGTase mutant as described above; and a method of producing the CGTase mutant by using the same.
Inventors:
NAKAGAWA YOSHINORI (JP)
TAKADA MASAYASU (JP)
OGAWA KOICHI (JP)
HATADA YUJI (JP)
HORIKOSHI HIROKI (JP)
TAKADA MASAYASU (JP)
OGAWA KOICHI (JP)
HATADA YUJI (JP)
HORIKOSHI HIROKI (JP)
Application Number:
PCT/JP2007/061391
Publication Date:
December 13, 2007
Filing Date:
June 05, 2007
Export Citation:
Assignee:
JAPAN MAIZE PROD (JP)
JAPAN AGENCY MARINE EARTH SCI (JP)
NAKAGAWA YOSHINORI (JP)
TAKADA MASAYASU (JP)
OGAWA KOICHI (JP)
HATADA YUJI (JP)
HORIKOSHI HIROKI (JP)
JAPAN AGENCY MARINE EARTH SCI (JP)
NAKAGAWA YOSHINORI (JP)
TAKADA MASAYASU (JP)
OGAWA KOICHI (JP)
HATADA YUJI (JP)
HORIKOSHI HIROKI (JP)
International Classes:
C12N15/54; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12P21/02
Domestic Patent References:
| WO2005003337A1 | 2005-01-13 |
Foreign References:
| JPH05501059A | 1993-03-04 | |||
| JP2003102489A | 2003-04-08 | |||
| JP2003531564A | 2003-10-28 | |||
| JP2004305176A | 2004-11-04 | |||
| JP2003070474A | 2003-03-11 | |||
| JP2006160175A | 2006-06-22 | |||
| JP2003531564A | 2003-10-28 | |||
| JPH11503906A | 1999-04-06 | |||
| JPH1033187A | 1998-02-10 | |||
| JP2003102489A | 2003-04-08 | |||
| JP2001327299A | 2001-11-27 | |||
| JP2001327284A | 2001-11-27 |
Other References:
ARRIZUBIETA M.J. ET AL.: "Increased thermal resistance and modification of the catalytic properties of a beta-glucosidase by random mutagenesis and in vitro recombination", J. BIOL. CHEM., vol. 275, no. 37, 2000, pages 28843 - 28848, XP003020185
NAKAGAWA Y. ET AL.: "Ko Alkali-sei Bacillus clarkii 7364 Yurai gamma-CGTase no Acceptor Bui ni Ichi suru Ala223 no Bui Tokuiteki Hen'i ni yoru CD Seisei Hanno eno Eikyo", NIPPON NOGEI KAGAKUKAI TAIKAI KOEN YOSHISHU, vol. 2007, no. 64, 5 March 2007 (2007-03-05), pages 64 + ABSTR. NO. 2A16A05, XP003020186
See also references of EP 2031066A4
AKIRA NAKAMURA; KEIKO HAGA; KUNIO YAMANE, BIOCHEMISTRY, vol. 32, 1993, pages 6624 - 6631
MICHIO KUBOTA ET AL., OYO TOSHITSU KAGAKU, vol. 41, no. 2, 1994, pages 245 - 253
NAKAGAWA Y. ET AL.: "Ko Alkali-sei Bacillus clarkii 7364 Yurai gamma-CGTase no Acceptor Bui ni Ichi suru Ala223 no Bui Tokuiteki Hen'i ni yoru CD Seisei Hanno eno Eikyo", NIPPON NOGEI KAGAKUKAI TAIKAI KOEN YOSHISHU, vol. 2007, no. 64, 5 March 2007 (2007-03-05), pages 64 + ABSTR. NO. 2A16A05, XP003020186
See also references of EP 2031066A4
AKIRA NAKAMURA; KEIKO HAGA; KUNIO YAMANE, BIOCHEMISTRY, vol. 32, 1993, pages 6624 - 6631
MICHIO KUBOTA ET AL., OYO TOSHITSU KAGAKU, vol. 41, no. 2, 1994, pages 245 - 253
Attorney, Agent or Firm:
SIKs & Co. (Kyobashi-Nisshoku Bldg.8-7, Kyobashi 1-chom, Chuo-ku Tokyo 31, JP)
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