Title:
MUTANT HISTIDINE DECARBOXYLASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/168801
Kind Code:
A1
Abstract:
Provided is a mutant histidine decarboxylase suitable for practical use. More specifically, provided are a mutant histidine decarboxylase and use of the same, said mutant histidine decarboxylase having a mutation in at least one amino acid residue in wild type histidine decarboxylase and showing a higher histidine decarboxylase activity and/or a higher stability than the wild type histidine decarboxylase. The mutant histidine decarboxylase has motifs (1) to (6) and it is preferred that the mutation occurs in amino acid residue(s) in at least one of these motifs. It is also preferred that the mutant histidine decarboxylase has the mutation in at least one amino acid residue in the amino acid sequence represented by SEQ ID NO: 3 or a sequence homologous thereto.
Inventors:
YAMAGUCHI HIROKI (JP)
SUGIKI MASAYUKI (JP)
NAKATA KUNIO (JP)
SUGIKI MASAYUKI (JP)
NAKATA KUNIO (JP)
Application Number:
PCT/JP2018/009572
Publication Date:
September 20, 2018
Filing Date:
March 12, 2018
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N15/60; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88
Domestic Patent References:
| WO2015137418A1 | 2015-09-17 | |||
| WO2016103761A1 | 2016-06-30 | |||
| WO2009081991A1 | 2009-07-02 | |||
| WO2016056631A1 | 2016-04-14 |
Foreign References:
| JP2016512021A | 2016-04-25 | |||
| JPS57141289A | 1982-09-01 | |||
| JP2001242174A | 2001-09-07 |
Other References:
DATABASE UniProtKB [O] 10 February 2009 (2009-02-10), "Protein: Histidine decarboxylase, Organism: Acinetobacter baumannii", XP055545853, Database accession no. B7GZJ8
DATABASE UniProtKB [O] 14 May 2014 (2014-05-14), "Protein: Histidine decarboxylase, Organism: Klebsiella pneumoniae", XP055545856, Database accession no. W9B1E6
DATABASE UniProtKB [O] 23 January 2007 (2007-01-23), "Protein: Histidine decarboxylase,Organism: Morganella morganii (Proteus morganii", XP055545857, Database accession no. P05034
DATABASE UniProt [O] 11 November 2015 (2015-11-11), "Protein: Histidine decarboxylase, Organism: Xenorhabdus sp. GDc328", XP055545862, Database accession no. A0A0M0T5M7
DATABASE UniProt 23 January 2007 (2007-01-23), "Protein: Histidine decarboxylase, Organism: Klebsiella aerogenes", XP055545866, Database accession no. P28577
DATABASE UniProt [O] 14 October 2015 (2015-10-14), "Protein: Histidine decarboxylase, Organism: Chromobacterium sp. LK11", XP055545868, Database accession no. A0A0J6NJE2
DATABASE Nucleotide 10 March 2014 (2014-03-10), "Morganella morganii subsp. morganii strain DSM 30164 histidine decarboxy lase (hdc", XP055545873, Database accession no. KC771251
DATABASE Nucleotide 3 March 2007 (2007-03-03), "Photobacterium phosphoreum hdc gene for histidine decarboxy lase", XP055545878, Database accession no. AB259288
VAN POELJE P. D. ET AL.: "Site-directed alteration of the active-site residues of histidine decarboxylase from Clostridium perfringens", BIOCHEMISTRY, vol. 29, 1990, pages 10413 - 10418, XP055545892
VAALER G. L. ET AL.: "Pyridoxal 5'-phosphate dependent histidine decarboxylase: overproduction, purification, biosynthesis of soluble site-directed mutant proteins, and replacement of conserved residues", BIOCHEMISTRY, vol. 28, 1989, pages 7306 - 7313, XP055545895
ISHII S. ET AL.: "Functionally important residues of aromatic L-amino acid decarboxylase probed by sequence alignment and site-directed mutagenesis", J. BIOCHEM., vol. 120, no. 2, 1 August 1996 (1996-08-01), pages 369 - 376, XP055625009, ISSN: 0021-924X, DOI: 10.1093/oxfordjournals.jbchem.a021422
LEVY H: "Histidinemia", ORPHANET, 2004
KAWAI Y ET AL.: "Molecular characterization of histidinemia: identification of four missense mutations in the histidase gene", HUM GENET., vol. 116, 2005, pages 340 - 346, XP036030761, DOI: 10.1007/s00439-004-1232-5
IKUNOSUKE SAKURABAYASHIKAZUNARI KUMASAKA: "The latest Clinical inspection items encyclopedia", 2008, ISHIYAKU PUB,INC., article "Saishin Rinsho kensa koumoku jiten", pages: 205
HISAMATSU T ET AL., PLOS ONE, vol. 7, no. 1, 2012, pages e31131
MIYAGI Y ET AL., PLOS ONE, vol. 6, no. 9, 2011, pages e24143
HASEBE Y ET AL., SENSORS AND ACTUATORS B, vol. 66, 2000, pages 12 - 15
"Enterobacter aerogenes", MOLECULAR MICROBIOLOGY, vol. 79, no. 4, 2011, pages 861 - 871
J. APPL. MICROBIOL., vol. 104, no. 1, 2008, pages 194 - 203
KARLINALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
PEARSON, METHODS ENZYMOL., vol. 183, 1990, pages 63
"Molecular Cloning: A Laboratory Manual", 15 January 2001, COLD SPRING HARBOR PRESS
DATABASE UniProtKB [O] 14 May 2014 (2014-05-14), "Protein: Histidine decarboxylase, Organism: Klebsiella pneumoniae", XP055545856, Database accession no. W9B1E6
DATABASE UniProtKB [O] 23 January 2007 (2007-01-23), "Protein: Histidine decarboxylase,Organism: Morganella morganii (Proteus morganii", XP055545857, Database accession no. P05034
DATABASE UniProt [O] 11 November 2015 (2015-11-11), "Protein: Histidine decarboxylase, Organism: Xenorhabdus sp. GDc328", XP055545862, Database accession no. A0A0M0T5M7
DATABASE UniProt 23 January 2007 (2007-01-23), "Protein: Histidine decarboxylase, Organism: Klebsiella aerogenes", XP055545866, Database accession no. P28577
DATABASE UniProt [O] 14 October 2015 (2015-10-14), "Protein: Histidine decarboxylase, Organism: Chromobacterium sp. LK11", XP055545868, Database accession no. A0A0J6NJE2
DATABASE Nucleotide 10 March 2014 (2014-03-10), "Morganella morganii subsp. morganii strain DSM 30164 histidine decarboxy lase (hdc", XP055545873, Database accession no. KC771251
DATABASE Nucleotide 3 March 2007 (2007-03-03), "Photobacterium phosphoreum hdc gene for histidine decarboxy lase", XP055545878, Database accession no. AB259288
VAN POELJE P. D. ET AL.: "Site-directed alteration of the active-site residues of histidine decarboxylase from Clostridium perfringens", BIOCHEMISTRY, vol. 29, 1990, pages 10413 - 10418, XP055545892
VAALER G. L. ET AL.: "Pyridoxal 5'-phosphate dependent histidine decarboxylase: overproduction, purification, biosynthesis of soluble site-directed mutant proteins, and replacement of conserved residues", BIOCHEMISTRY, vol. 28, 1989, pages 7306 - 7313, XP055545895
ISHII S. ET AL.: "Functionally important residues of aromatic L-amino acid decarboxylase probed by sequence alignment and site-directed mutagenesis", J. BIOCHEM., vol. 120, no. 2, 1 August 1996 (1996-08-01), pages 369 - 376, XP055625009, ISSN: 0021-924X, DOI: 10.1093/oxfordjournals.jbchem.a021422
LEVY H: "Histidinemia", ORPHANET, 2004
KAWAI Y ET AL.: "Molecular characterization of histidinemia: identification of four missense mutations in the histidase gene", HUM GENET., vol. 116, 2005, pages 340 - 346, XP036030761, DOI: 10.1007/s00439-004-1232-5
IKUNOSUKE SAKURABAYASHIKAZUNARI KUMASAKA: "The latest Clinical inspection items encyclopedia", 2008, ISHIYAKU PUB,INC., article "Saishin Rinsho kensa koumoku jiten", pages: 205
HISAMATSU T ET AL., PLOS ONE, vol. 7, no. 1, 2012, pages e31131
MIYAGI Y ET AL., PLOS ONE, vol. 6, no. 9, 2011, pages e24143
HASEBE Y ET AL., SENSORS AND ACTUATORS B, vol. 66, 2000, pages 12 - 15
"Enterobacter aerogenes", MOLECULAR MICROBIOLOGY, vol. 79, no. 4, 2011, pages 861 - 871
J. APPL. MICROBIOL., vol. 104, no. 1, 2008, pages 194 - 203
KARLINALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
PEARSON, METHODS ENZYMOL., vol. 183, 1990, pages 63
"Molecular Cloning: A Laboratory Manual", 15 January 2001, COLD SPRING HARBOR PRESS
Attorney, Agent or Firm:
SAKAI INTERNATIONAL PATENT OFFICE (JP)
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