Title:
MUTANT MAD7 PROTEIN
Document Type and Number:
WIPO Patent Application WO/2023/145833
Kind Code:
A1
Abstract:
Provided is a mutant MAD7 protein demonstrating enhanced breakage activity to a target site. This mutant MAD7 protein contains an amino acid sequence B resulting from mutation of an amino acid sequence A with Sequence Number 1, the amino acid sequence B including amino acid substitution mutations at K169 and D529 in the amino acid sequence A.
Inventors:
HOZUMI SHUNYA (JP)
NISHIMURA KOICHI (JP)
MASUDA OKURA (JP)
TAKADERA SATORU (JP)
KAINUMA RISA (JP)
YAMASHITA YUKIKO (JP)
SAWATSUBASHI SHUN (JP)
NISHIMURA KOICHI (JP)
MASUDA OKURA (JP)
TAKADERA SATORU (JP)
KAINUMA RISA (JP)
YAMASHITA YUKIKO (JP)
SAWATSUBASHI SHUN (JP)
Application Number:
PCT/JP2023/002475
Publication Date:
August 03, 2023
Filing Date:
January 26, 2023
Export Citation:
Assignee:
SETSUROTECH INC (JP)
International Classes:
C12N15/55; A61K35/12; A61K38/46; A61K48/00; A61P43/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/09
Domestic Patent References:
| WO2021257716A2 | 2021-12-23 | |||
| WO2020086475A1 | 2020-04-30 | |||
| WO2020086475A1 | 2020-04-30 |
Foreign References:
| JP2017184639A | 2017-10-12 | |||
| JP2022100914A | 2022-07-06 |
Other References:
LIN QIUPENG; ZHU ZIXU; LIU GUANWEN; SUN CHAO; LIN DEXING; XUE CHENXIAO; LI SHENGNAN; ZHANG DANDAN; GAO CAIXIA; WANG YANPENG; QIU J: "Genome editing in plants with MAD7 nuclease", JOURNAL OF GENETICS AND GENOMICS, ELSEVIER LTD., AMSTERDAM, NL, vol. 48, no. 6, 3 May 2021 (2021-05-03), AMSTERDAM, NL , pages 444 - 451, XP086758272, ISSN: 1673-8527, DOI: 10.1016/j.jgg.2021.04.003
KARLIN SALTSCHUL SF: "Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes", PROC NATL ACAD SCI USA., vol. 87, 1990, pages 2264 - 2268, XP001030853, DOI: 10.1073/pnas.87.6.2264
KARLIN SALTSCHUL SF: "Applications and statistics for multiple high-scoring segments in molecular sequences", PROC NATL ACAD SCI USA., vol. 90, 1993, pages 5873 - 7, XP001030852, DOI: 10.1073/pnas.90.12.5873
KARLIN SALTSCHUL SF: "Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes", PROC NATL ACAD SCI USA., vol. 87, 1990, pages 2264 - 2268, XP001030853, DOI: 10.1073/pnas.87.6.2264
KARLIN SALTSCHUL SF: "Applications and statistics for multiple high-scoring segments in molecular sequences", PROC NATL ACAD SCI USA., vol. 90, 1993, pages 5873 - 7, XP001030852, DOI: 10.1073/pnas.90.12.5873
Attorney, Agent or Firm:
IP LAW FIRM LEXCEED TECH (JP)
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