Title:
MUTANT STRAIN OF PARACOCCUS DENITRIFICANS (PDΔζ) WITH INCREASED DENITRIFYING ACTIVITY, AND SPRECTROPHOTOMETRIC METHOD FOR MEASURING THE DENITRIFYING ACTIVITY IN REAL TIME
Document Type and Number:
WIPO Patent Application WO/2019/117698
Kind Code:
A3
Abstract:
The invention relates to a mutant strain of Paracoccus denitrificans with a greater denitrifying metabolism or activity, providing greater speeds of uptake of NO3 and NO2 with the use of a lower quantity of bacterial biomass for the effective removal of said nitrogenated compounds which are residual contaminants in treated water and in some foods, wherein said greater level of denitrifying activity is obtained by completely removing the gene of the subunit ζ as an inhibitor of the F1FO-ATPase, with the denitrification via Paracoccus denitrificans being carried out in anaerobic conditions.
Inventors:
GARCÍA TREJO JOSÉ DE JESÚS (MX)
CEVALLOS GAOS MIGUEL ÁNGEL CARLOS (MX)
MENDOZA HOFFMAN FRANCISCO GUILLERMO (MX)
CEVALLOS GAOS MIGUEL ÁNGEL CARLOS (MX)
MENDOZA HOFFMAN FRANCISCO GUILLERMO (MX)
Application Number:
PCT/MX2018/000144
Publication Date:
August 08, 2019
Filing Date:
December 13, 2018
Export Citation:
Assignee:
UNIV MEXICO NAC AUTONOMA (MX)
International Classes:
C12N1/20; C12N1/21; C12R1/01
Other References:
MENDOZA-HOFFMANN FRANCISCO ET AL.: "The Biological Role of the zeta Subunit as Unidirectional Inhibitor of the FIFO-ATPase of Paracoccus denitrificans", CELL REPORTS, vol. 22, no. 4, 23 January 2018 (2018-01-23), pages 1067 - 1078, XP055628432, ISSN: 2211-1247
VARGHESE FEBIN ET AL.: "Deleting the IFl-like zeta subunit from Paracoccus denitrificans ATP synthase is not sufficient to activate ATP hydrolysis", OPEN BIOLOGY, vol. 8, no. 1, 31 December 2017 (2017-12-31) - 24 January 2018 (2018-01-24), pages 170206, XP055628437, ISSN: 2046-2441
ZARCO-ZAVALA MARIEL ET AL.: "Unidirectional regulation of the FIFO-ATP synthase nanomotor by the zeta pawl-ratchet inhibitor protein of Paracoccus denitrificans and related alpha-proteobacteria", BIOCHIMICA ET BIOPHYSICA ACTA . BIOENERGETICS, vol. 1859, no. 9, 8 June 2018 (2018-06-08), AMSTERDAM, NL, pages 762 - 774, XP085435377, ISSN: 0005-2728, doi:10.1016/j.bbabio.2018.06.005
GARCIA-TREJO JOSE J ET AL.: "The Inhibitory Mechanism of the zet; Subunit of the FIFO-ATPase Nanomotor of Paracoccus denitrificans and Related alpha-Proteobacteria", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 291, no. 2, 8 January 2016 (2016-01-08), pages 538 - 546, XP055628440
ZUMFT WALTER G. ET AL.: "Cell biology and molecular basis of denitrification- 564- Bacterial Metabolism of NO '', ''Topology of the Denitrification System", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 61, no. 4, 30 November 1997 (1997-11-30) - December 1997 (1997-12-01), pages 533 - 616, XP055628443, ISSN: 1092-2172
MORALES-RIOS EDGAR ET AL.: "A novel 11-kDa inhibitory subunit in the FIFO ATP synthase of Paracoccus denitrificans and related alpha-proteobacteria", FASEB JOURNAL FEB 2010, vol. 24, no. 2, 25 September 2009 (2009-09-25) - February 2010 (2010-02-01), pages 599 - 608, XP055628444, ISSN: 0892-6638
ZARCO-ZAVALA MARIEL ET AL.: "The zeta subunit of the FIFO-ATI synthase of alpha-proteobacteria controls rotation of the nanomotor with a different structure", FASEB JOURNAL JUN, vol. 28, no. 5, 12 February 2014 (2014-02-12) - May 2014 (2014-05-01), pages 2146 - 2157, XP055628447, ISSN: 0892-6638
VARGHESE FEBIN ET AL.: "Deleting the IFl-like zeta subunit from Paracoccus denitrificans ATP synthase is not sufficient to activate ATP hydrolysis", OPEN BIOLOGY, vol. 8, no. 1, 31 December 2017 (2017-12-31) - 24 January 2018 (2018-01-24), pages 170206, XP055628437, ISSN: 2046-2441
ZARCO-ZAVALA MARIEL ET AL.: "Unidirectional regulation of the FIFO-ATP synthase nanomotor by the zeta pawl-ratchet inhibitor protein of Paracoccus denitrificans and related alpha-proteobacteria", BIOCHIMICA ET BIOPHYSICA ACTA . BIOENERGETICS, vol. 1859, no. 9, 8 June 2018 (2018-06-08), AMSTERDAM, NL, pages 762 - 774, XP085435377, ISSN: 0005-2728, doi:10.1016/j.bbabio.2018.06.005
GARCIA-TREJO JOSE J ET AL.: "The Inhibitory Mechanism of the zet; Subunit of the FIFO-ATPase Nanomotor of Paracoccus denitrificans and Related alpha-Proteobacteria", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 291, no. 2, 8 January 2016 (2016-01-08), pages 538 - 546, XP055628440
ZUMFT WALTER G. ET AL.: "Cell biology and molecular basis of denitrification- 564- Bacterial Metabolism of NO '', ''Topology of the Denitrification System", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 61, no. 4, 30 November 1997 (1997-11-30) - December 1997 (1997-12-01), pages 533 - 616, XP055628443, ISSN: 1092-2172
MORALES-RIOS EDGAR ET AL.: "A novel 11-kDa inhibitory subunit in the FIFO ATP synthase of Paracoccus denitrificans and related alpha-proteobacteria", FASEB JOURNAL FEB 2010, vol. 24, no. 2, 25 September 2009 (2009-09-25) - February 2010 (2010-02-01), pages 599 - 608, XP055628444, ISSN: 0892-6638
ZARCO-ZAVALA MARIEL ET AL.: "The zeta subunit of the FIFO-ATI synthase of alpha-proteobacteria controls rotation of the nanomotor with a different structure", FASEB JOURNAL JUN, vol. 28, no. 5, 12 February 2014 (2014-02-12) - May 2014 (2014-05-01), pages 2146 - 2157, XP055628447, ISSN: 0892-6638
Attorney, Agent or Firm:
FIGUEROA PÉREZ, Martha (MX)
Download PDF: