Title:
MUTANT STRAIN OF TYPE 3 DUCK HEPATOVIRUS CH-P60-117C STRAIN AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/258757
Kind Code:
A1
Abstract:
Provided are a mutant strain of type 3 duck hepatovirus CH-P60-117C strain and a construction method therefor. Specifically, the nucleotide at position 117 of the genome 5'UTR of type 3 virulent duck hepatovirus strain is mutated from A to C; the nucleotide at position 1142 is mutated from T to A, such that the amino acid at position 164 of the VP0 protein of the virus is mutated from tyrosine of the parent strain to asparagine; and the nucleotide at position 4334 is mutated from C to A, so that the amino acid at position 71 of the viral 2C protein is mutated from leucine of the parent strain to isoleucine. The CH-P60-117C strain has good immunogenicity and genetic stability, and is not pathogenic to ducklings.
Inventors:
CHENG ANCHUN (CN)
WEN XINGJIAN (CN)
WANG MINGSHU (CN)
WU LIPING (CN)
WEN XINGJIAN (CN)
WANG MINGSHU (CN)
WU LIPING (CN)
Application Number:
PCT/CN2019/125293
Publication Date:
December 30, 2020
Filing Date:
December 13, 2019
Export Citation:
Assignee:
UNIV SICHUAN AGRICULTURAL (CN)
International Classes:
A61K39/125; C12N7/01; C12N15/87; A61P31/14; C12R1/93
Foreign References:
| CN110295149A | 2019-10-01 | |||
| CN110283835A | 2019-09-27 | |||
| CN110295180A | 2019-10-01 | |||
| CN109797139A | 2019-05-24 |
Other References:
XINGJIAN WEN,JINLONG GUO,DI SUN,MINGSHU WANG,DIAN CAO,ANCHUN CHENG,DEKANG ZHU,MAFENG LIU,XINXIN ZHAO,QIAO YANG,SHUN CHEN,RENYONG J: "Mutations in VP0 and 2C Proteins of Duck Hepatitis A Virus Type 3 Attenuate Viral Infection and Virulence", VACCINES, vol. 7, no. 111, 11 September 2019 (2019-09-11), pages 1 - 19, XP055773984, ISSN: 2076-393X, DOI: 10.3390/vaccines7030111
DATABASE Nucleoide 10 June 2019 (2019-06-10), ANONYMOUS: "Duck hepatitis A virus 3 strain CH-P60, complete genome", XP055773992, retrieved from NCBI Database accession no. MH752742.1
OU XUMIN , WANG MINGSHU, MAO SAI, CAO JINGYU, CHENG ANCHUN, ZHU DEKANG, CHEN SHUN, JIA RENYONG, LIU MAFENG, YANG QIAO, WU YING, ZH: "Incompatible Translation Drives a Convergent Evolution and Viral Attenuation During the Development of Live Attenuated Vaccine", FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, vol. 8, 249, 18 July 2018 (2018-07-18), pages 1 - 11, XP055773995, ISSN: 2235-2988, DOI: 10.3389/fcimb.2018.00249
DATABASE Nucleoide 10 June 2019 (2019-06-10), ANONYMOUS: "Duck hepatitis A virus 3 strain CH-P60, complete genome", XP055773992, retrieved from NCBI Database accession no. MH752742.1
OU XUMIN , WANG MINGSHU, MAO SAI, CAO JINGYU, CHENG ANCHUN, ZHU DEKANG, CHEN SHUN, JIA RENYONG, LIU MAFENG, YANG QIAO, WU YING, ZH: "Incompatible Translation Drives a Convergent Evolution and Viral Attenuation During the Development of Live Attenuated Vaccine", FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, vol. 8, 249, 18 July 2018 (2018-07-18), pages 1 - 11, XP055773995, ISSN: 2235-2988, DOI: 10.3389/fcimb.2018.00249
Attorney, Agent or Firm:
CHENGDU ZHENGHUA PATENT AGENCY (CN)
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