Title:
MUTANT TETRAPRENYL-β-CURCUMENE CYCLASE AND METHOD FOR PRODUCING AMBREIN USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/193806
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for producing ambrein by which ambrein can be easily and efficiently obtained. This problem can be solved by a mutant tetraprenyl-β-curcumene cyclase in which aspartic acid that is the fourth amino acid residue of a DXDD motif is substituted by an amino acid other than aspartic acid and the seventh amino acid of a (D/N)G(T/L)(L/F/Y)(Y/F)SY motif is substituted by an amino acid other than tyrosine, said mutant tetraprenyl-β-curcumene cyclase: (a) having nine specific motifs; (b) having 40% or higher sequence identity with the amino acid sequence represented by SEQ ID NO: 1; and (c) showing an ambrein synthesis activity using 8α-hydroxypolypoda-13,17,21-triene as a substrate.
Inventors:
SATO TSUTOMU (JP)
TAKEHANA TOSHIHIKO (JP)
KOIKE SEIJI (JP)
TAKEHANA TOSHIHIKO (JP)
KOIKE SEIJI (JP)
Application Number:
PCT/JP2021/012483
Publication Date:
September 30, 2021
Filing Date:
March 25, 2021
Export Citation:
Assignee:
UNIV NIIGATA (JP)
ADEKA CORP (JP)
ADEKA CORP (JP)
International Classes:
C12P17/06; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N15/52; C12N15/63
Domestic Patent References:
| WO2019045058A1 | 2019-03-07 | |||
| WO2017150695A1 | 2017-09-08 | |||
| WO2015033746A1 | 2015-03-12 |
Other References:
SATO, TSUTOMU ET AL.: "Catalytic Function of the Residues of Phenylalanine and Tyrosine Conserved in Squalene-Hopene Cyclases", BIOSCI.BIOTECHNOL.BIOCHEM., vol. 65, no. 10, 2001, pages 2233 - 2242, XP055325755, DOI: 10.1271/bbb.65.2233
MOSER SANDRA, LEITNER ERICH, PLOCEK THOMAS J., VANHESSCHE KOENRAAD, PICHLER HARALD: "Engineering of Saccharomyces cerevisiae for the production of (+)-ambrein", YEAST, vol. 37, no. 1, 2020, pages 163 - 172, XP055861861
KE DI, CAIYIN QINGGELE, ZHAO FANGLONG, LIU TING, LU WENYU: "Heterologous biosynthesis of triterpenoid ambrein in engineered Escherichia coli", BIOTECHNOL LETT, vol. 40, 2018, pages 399 - 404, XP036426422
MOSER SANDRA, LEITNER ERICH, PLOCEK THOMAS J., VANHESSCHE KOENRAAD, PICHLER HARALD: "Engineering of Saccharomyces cerevisiae for the production of (+)-ambrein", YEAST, vol. 37, no. 1, 2020, pages 163 - 172, XP055861861
KE DI, CAIYIN QINGGELE, ZHAO FANGLONG, LIU TING, LU WENYU: "Heterologous biosynthesis of triterpenoid ambrein in engineered Escherichia coli", BIOTECHNOL LETT, vol. 40, 2018, pages 399 - 404, XP036426422
Attorney, Agent or Firm:
YAMAGUCHI Kenjiro et al. (JP)
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