Title:
MUTANT TYPE 2-DEOXY-SCYLLO-INOSOSE SYNTHASE
Document Type and Number:
WIPO Patent Application WO/2018/180568
Kind Code:
A1
Abstract:
A polypeptide that has a specific amino acid mutation at at least one of amino acid residues at the positions 14, 37, 290, 293 and 319, from the N-end, of the amino acid sequence represented by SEQ ID NO: 1 in the amino acid sequence represented by SEQ ID NO: 1 or a sequence analogous thereto; a polynucleotide that has a base sequence encoding the amino acid sequence of the aforesaid polypeptide; an expression cassette; a vector; a transformant; a method for producing the aforesaid polypeptide; and a method for producing 2-deoxy-scyllo-inosose.
Inventors:
TAKAKU HIROAKI (JP)
YAMAZAKI HARUTAKE (JP)
WADA MITSUFUMI (JP)
MIYAZAWA DAISUKE (JP)
YAMAZAKI HARUTAKE (JP)
WADA MITSUFUMI (JP)
MIYAZAWA DAISUKE (JP)
Application Number:
PCT/JP2018/010349
Publication Date:
October 04, 2018
Filing Date:
March 15, 2018
Export Citation:
Assignee:
THE NIIGATA INSTITUTE OF SCIENCE AND TECH (JP)
MITSUI CHEMICALS INC (JP)
MITSUI CHEMICALS INC (JP)
International Classes:
C12N15/10; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12P7/26; C12P21/02
Domestic Patent References:
| WO2006109479A1 | 2006-10-19 | |||
| WO2006109479A1 | 2006-10-19 | |||
| WO2010053052A1 | 2010-05-14 |
Foreign References:
| JP2005053899A | 2005-03-03 | |||
| JP2000236881A | 2000-09-05 | |||
| JP2014064513A | 2014-04-17 | |||
| JP2013135697A | 2013-07-11 | |||
| JP2017061572A | 2017-03-30 |
Other References:
TAKAKU, HIROAKI ET AL.: "Laboratory of applied microbiology", NIIGATA UNIVERSITY OF PHARMACY AND APPLIED LIFE SCIENCES ANNUAL RESEARCH REPORT 2014, 2014, pages 31 - 37, XP009516793
WAKISAKA, NAOKI ET AL.: "Acquiring highly active mutants of aromatic compound precursor synthesis enzymes by evolutionary engineering techniques and the optimization of producing precursor synthesis enzymes", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2011. JSBBA ANNUAL MEETING REPORT, vol. 2011, no. 23, 5 March 2011 (2011-03-05), pages 1, XP009516655
SUZUKI, RYOKO ET AL.: "2C23a04 Enhancement of 2-deoxy-scyllo-inosose (DOI) synthase activity by amino acid substitution", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2017; MARCH 17-20, 2017; KYOTO, JAPAN. JSBBA ANNUAL MEETING REPORT, vol. 2017, 5 March 2017 (2017-03-05), XP009516652, ISSN: 2186-7976
NANGO ERIKO ET AL.: "Active site mapping of 2- deoxy-scyllo-inosose synthase, the key starter enzyme for the biosynthesis of 2-deoxystreptamine. Mechanism-based inhibition and identification of Lysine-141 as the entrapped nucleophile", JOURNAL OF ORGANIC CHEMISTRY, vol. 69, no. 3, 6 February 2004 (2004-02-06), pages 593 - 600, XP055558145
NANGO ERIKO ET AL.: "Structure of 2-deoxy-scyllo- inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside based inhibitor and NAD+", PROTEINS, vol. 70, no. 2, 24 August 2008 (2008-08-24), pages 517 - 527, XP002725704
KAKINUMA ET AL., TETRAHEDRON LETTERS, vol. 41, 2000, pages 1935
KUDO ET AL., J. ANTIBIOT., vol. 52, 1999, pages 559
HIRAYAMA ET AL., J. ANTIBIOT., vol. 58, 2005, pages 766
SUBBA ET AL., MOL. CELLS, vol. 20, 2005, pages 90
KHAREL ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 429, 2004, pages 204
STREPTOMYCES KANAMYCETICUS, J. ANTIBIOT., vol. 57, 2004, pages 436
KHAREL ET AL., FEMS MICROBIOL. LETT., vol. 230, 2004, pages 185
HIRAYAMA ET AL., J. ANTIBIOT., vol. 59, 2006, pages 358
TAMEGAI ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 74, 2010, pages 1215
KOGURE ET AL., J. BIOTECHNOL., vol. 129, 2007, pages 502
KRAMER, W.FRITA, H. J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
"PCR Technology", 1989, STOCKTON PRESS
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
WAKISAKA, NAOKI ET AL.: "Acquiring highly active mutants of aromatic compound precursor synthesis enzymes by evolutionary engineering techniques and the optimization of producing precursor synthesis enzymes", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2011. JSBBA ANNUAL MEETING REPORT, vol. 2011, no. 23, 5 March 2011 (2011-03-05), pages 1, XP009516655
SUZUKI, RYOKO ET AL.: "2C23a04 Enhancement of 2-deoxy-scyllo-inosose (DOI) synthase activity by amino acid substitution", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2017; MARCH 17-20, 2017; KYOTO, JAPAN. JSBBA ANNUAL MEETING REPORT, vol. 2017, 5 March 2017 (2017-03-05), XP009516652, ISSN: 2186-7976
NANGO ERIKO ET AL.: "Active site mapping of 2- deoxy-scyllo-inosose synthase, the key starter enzyme for the biosynthesis of 2-deoxystreptamine. Mechanism-based inhibition and identification of Lysine-141 as the entrapped nucleophile", JOURNAL OF ORGANIC CHEMISTRY, vol. 69, no. 3, 6 February 2004 (2004-02-06), pages 593 - 600, XP055558145
NANGO ERIKO ET AL.: "Structure of 2-deoxy-scyllo- inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside based inhibitor and NAD+", PROTEINS, vol. 70, no. 2, 24 August 2008 (2008-08-24), pages 517 - 527, XP002725704
KAKINUMA ET AL., TETRAHEDRON LETTERS, vol. 41, 2000, pages 1935
KUDO ET AL., J. ANTIBIOT., vol. 52, 1999, pages 559
HIRAYAMA ET AL., J. ANTIBIOT., vol. 58, 2005, pages 766
SUBBA ET AL., MOL. CELLS, vol. 20, 2005, pages 90
KHAREL ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 429, 2004, pages 204
STREPTOMYCES KANAMYCETICUS, J. ANTIBIOT., vol. 57, 2004, pages 436
KHAREL ET AL., FEMS MICROBIOL. LETT., vol. 230, 2004, pages 185
HIRAYAMA ET AL., J. ANTIBIOT., vol. 59, 2006, pages 358
TAMEGAI ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 74, 2010, pages 1215
KOGURE ET AL., J. BIOTECHNOL., vol. 129, 2007, pages 502
KRAMER, W.FRITA, H. J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
"PCR Technology", 1989, STOCKTON PRESS
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
NAKAJIMA, Jun et al. (JP)
Download PDF: