Title:
MUTANT XYLANASE, MANUFACTURING METHOD AND USE THEREFOR, AND METHOD FOR MANUFACTURING SACCHARIFIED LIGNOCELLULOSE
Document Type and Number:
WIPO Patent Application WO/2013/077432
Kind Code:
A1
Abstract:
The present invention addresses the following problems: providing an inexpensive, efficient lignocellulose saccharification method using a thermostable xylanase; and providing a mutant xylanase that has a substituted amino-acid residue, exhibits stable activity even under harsh conditions in which enzymes are easily deactivated, and has an initial reaction rate not much less than that of the corresponding wild-type xylanase. A lignocellulose saccharification method that includes bringing a lignocellulose feedstock into contact with a thermostable xylanase; and a mutant xylanase that has a substituted amino-acid residue, has an initial reaction rate at least 70% of that of the corresponding wild-type xylanase, and, after a 24-hour heat treatment at 50°C, exhibits xylanase activity at least 50% of that exhibited before said heat treatment.
Inventors:
YANAI HISAAKI (JP)
TAMAI HIROKI (JP)
OSABE MASAMI (JP)
YOKOYAMA FUMIKAZU (JP)
OKAKURA KAORU (JP)
INOUE ATSUSHI (JP)
TAMAI HIROKI (JP)
OSABE MASAMI (JP)
YOKOYAMA FUMIKAZU (JP)
OKAKURA KAORU (JP)
INOUE ATSUSHI (JP)
Application Number:
PCT/JP2012/080387
Publication Date:
May 30, 2013
Filing Date:
November 22, 2012
Export Citation:
Assignee:
MITSUI CHEMICALS INC (JP)
MEIJI SEIKA PHARMA CO LTD (JP)
MEIJI SEIKA PHARMA CO LTD (JP)
International Classes:
C12N15/56; A21D8/04; A23K1/165; C11D3/386; C12N1/15; C12N1/19; C12N1/21; C12N9/24; C12P19/14; D06L4/40
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Other References:
DATABASE GENBANK [online] 14 November 2006 (2006-11-14), ALCOCER,M.J. ET AL.: "ENDO-1,4-XYLANASE B [TALAROMYCES FUNICULOSUS]", XP055177435, retrieved from NCBI Database accession no. CAD33900
KRAMER, W.; FRITA, H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
"PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.; FRITA H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
VAN DEN HONDEL; C. A. M. J. J. ET AL.: "More gene Manipulations in Fungi", 1991, ACADEMIC PRESS, pages: 396 - 428
SAMBROOK, J.: "Molecular Cloning A Laboratory Manual, 3rd Edition", 2001, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 2784161A4
KRAMER, W.; FRITA, H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
"PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.; FRITA H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
VAN DEN HONDEL; C. A. M. J. J. ET AL.: "More gene Manipulations in Fungi", 1991, ACADEMIC PRESS, pages: 396 - 428
SAMBROOK, J.: "Molecular Cloning A Laboratory Manual, 3rd Edition", 2001, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 2784161A4
Attorney, Agent or Firm:
NAKAJIMA, Jun et al. (JP)
Nakajima 淳 (JP)
Nakajima 淳 (JP)
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Claims: