Title:
MUTANT YEAST AND METHOD FOR PRODUCING PROTEIN USING SAME
Document Type and Number:
WIPO Patent Application WO/2020/100910
Kind Code:
A1
Abstract:
The purpose of the present invention is to efficiently produce a cutinase-like enzyme by finding a peptidase or a protease that is involved in the decomposition of the cutinase-like enzyme and inhibiting the activity of the peptidase or the peptidase. By introducing a mutation to the gene of at least one type of peptidase or protease that is involved in the decomposition of PaE, a yeast is provided in which the activity of the peptidase or protease is inhibited.
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Inventors:
MORITA TOMOTAKE (JP)
KOIKE HIDEAKI (JP)
OMAE NATSUKI (JP)
KITAMOTO HIROKO (JP)
YAMASHITA YUKA (JP)
YARIMIZU TOHRU (JP)
KOIKE HIDEAKI (JP)
OMAE NATSUKI (JP)
KITAMOTO HIROKO (JP)
YAMASHITA YUKA (JP)
YARIMIZU TOHRU (JP)
Application Number:
PCT/JP2019/044380
Publication Date:
May 22, 2020
Filing Date:
November 12, 2019
Export Citation:
Assignee:
AIST (JP)
NAT AGRICULTURE & FOOD RES ORG (JP)
NAT AGRICULTURE & FOOD RES ORG (JP)
International Classes:
C12P21/02; C12N1/19; C12N9/16; C12N15/57
Other References:
WATANABE, TAKASHI ET AL.: "High-level recombinant protein production by the basidiomycetous yeast Pseudozyma antarctica under a xylose-inducible xylanase promoter", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,, vol. 100, no. 7, 23 December 2015 (2015-12-23), pages 3207 - 3217
GRAF, ALEXANDRA ET AL.: "Yeast systems biotechnology for the production of heterologous proteins", FEMS YEAST RES., vol. 9, no. 3, 1 April 2009 (2009-04-01), pages 335 - 348, XP055131911, DOI: 10.1111/j.1567-1364.2009.00507.x
ZHANG, YEWANG ET AL.: "The proteolytic systems and heterologous proteins degradation in the methylotrophic yeast Pichia pastoris", ANNALS OF MICROBIOLOGY, vol. 57, no. 4, 2007, pages 553 - 560, XP055080203
WU, MIN ET AL.: "Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 40, no. 6, 26 March 2013 (2013-03-26), pages 589 - 599
YU , XIAO-WEI ET AL.: "Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects", SCIENTIFIC REPORTS, vol. 7, no. 16249, 24 November 2017 (2017-11-24), pages 1 - 12, XP055505585
MORITA, TOMOTAKE ET AL.: "Genome and Transcriptome Analysis of the Basidiomycetous Yeast Pseudozyma antarctica Producing Extracellular Glycolipids , Mannosylerythritol Lipids", PLOS ONE, vol. 9, no. 2, 24 February 2014 (2014-02-24), pages e88544, XP055327616
YARIMIZU, TOHRU ET AL.: "Targeted gene replacement at the URA3 locus of the basidiomycetous yeast Pseudozyma antarctica and its transformation using lithium acetate treatment", YEAST, vol. 34, no. 12, 21 September 2017 (2017-09-21), pages 483 - 494, XP055708709
GRAF, ALEXANDRA ET AL.: "Yeast systems biotechnology for the production of heterologous proteins", FEMS YEAST RES., vol. 9, no. 3, 1 April 2009 (2009-04-01), pages 335 - 348, XP055131911, DOI: 10.1111/j.1567-1364.2009.00507.x
ZHANG, YEWANG ET AL.: "The proteolytic systems and heterologous proteins degradation in the methylotrophic yeast Pichia pastoris", ANNALS OF MICROBIOLOGY, vol. 57, no. 4, 2007, pages 553 - 560, XP055080203
WU, MIN ET AL.: "Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 40, no. 6, 26 March 2013 (2013-03-26), pages 589 - 599
YU , XIAO-WEI ET AL.: "Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects", SCIENTIFIC REPORTS, vol. 7, no. 16249, 24 November 2017 (2017-11-24), pages 1 - 12, XP055505585
MORITA, TOMOTAKE ET AL.: "Genome and Transcriptome Analysis of the Basidiomycetous Yeast Pseudozyma antarctica Producing Extracellular Glycolipids , Mannosylerythritol Lipids", PLOS ONE, vol. 9, no. 2, 24 February 2014 (2014-02-24), pages e88544, XP055327616
YARIMIZU, TOHRU ET AL.: "Targeted gene replacement at the URA3 locus of the basidiomycetous yeast Pseudozyma antarctica and its transformation using lithium acetate treatment", YEAST, vol. 34, no. 12, 21 September 2017 (2017-09-21), pages 483 - 494, XP055708709
Attorney, Agent or Firm:
TANAKA Shinichiro et al. (JP)
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