Title:
MUTATED ENZYME HAVING DEHYDROGENASE ACTIVITY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/068218
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a novel enzyme that exhibits a cholesterol dehydrogenase activity. Provided is a mutated enzyme that comprises an amino acid sequence derived from the amino acid sequence of a microorganism-derived cholesterol oxidase by substitution, into other amino acid(s), of one or more amino acids selected from the group consisting of: (1) an amino acid corresponding to the amino acid at the 113-position in the amino acid sequence represented by SEQ ID NO:1; (2) an amino acid corresponding to the amino acid at the 362-position in the amino acid sequence represented by SEQ ID NO:1; (3) an amino acid corresponding to the amino acid at the 402-position in the amino acid sequence represented by SEQ ID NO:1; (4) an amino acid corresponding to the amino acid at the 412-position in the amino acid sequence represented by SEQ ID NO:1; (5) an amino acid corresponding to the amino acid at the 468-position in the amino acid sequence represented by SEQ ID NO:1; (6) an amino acid corresponding to the amino acid at the 483-position in the amino acid sequence represented by SEQ ID NO:1; (7) an amino acid corresponding to the amino acid at the 518-position in the amino acid sequence represented by SEQ ID NO:1; and (8) an amino acid corresponding to the amino acid at the 519-position in the amino acid sequence represented by SEQ ID NO:1, and has a higher cholesterol dehydrogenase activity to cholesterol oxidase activity (CHDH activity/CHO activity) than the aforesaid microorganism-derived cholesterol oxidase.
Inventors:
YOSHIDA KAZUNORI (JP)
NISHIO KYOICHI (JP)
NISHIO KYOICHI (JP)
Application Number:
PCT/JP2015/080466
Publication Date:
May 06, 2016
Filing Date:
October 28, 2015
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N9/04; C12N1/15; C12N1/19; C12N1/21; C12N15/09; C12Q1/28; C12Q1/32; C12Q1/60
Domestic Patent References:
| WO2013026576A2 | 2013-02-28 |
Foreign References:
| JPH09500528A | 1997-01-21 | |||
| JP2003149247A | 2003-05-21 |
Other References:
KOJIMA, K. ET AL.: "Mutational analysis of the oxygen-binding site of cholesterol oxidase and its impact on dye-mediated dehydrogenase activity.", J. MOL. CATAL., B ENZYM., vol. 88, 2013, pages 41 - 46, XP028973547, ISSN: 1381-1177, DOI: doi:10.1016/j.molcatb.2012.11.001
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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