CRIMMINS GREGORY (US)
DE JESÚS DIAZ DENNISE (US)
WO2021092322A1 | 2021-05-14 | |||
WO2019213570A1 | 2019-11-07 | |||
WO2019213570A1 | 2019-11-07 | |||
WO2013169739A1 | 2013-11-14 | |||
WO1997048696A1 | 1997-12-24 | |||
WO1997048397A1 | 1997-12-24 | |||
WO2003080054A1 | 2003-10-02 | |||
WO2008025857A2 | 2008-03-06 | |||
WO2009109610A1 | 2009-09-11 |
US20200059329W | 2020-11-06 | |||
US9381253B2 | 2016-07-05 |
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CLAIMS What is claimed is: 1. A compound of Formula (I): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R2 is cyclopropylmethyl or 2,2-difluoroethyl; R3 is chloro, fluoro, -CN, -CF3, or -COOCH3; L1 is substituted or unsubstituted C2-4 alkylene, substituted or unsubstituted C2-4 alkenylene, substituted or unsubstituted C2-4 alkynylene, substituted or unsubstituted heteroalkylene, or substituted or unsubstituted carbocyclylene; Ring A is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R9 is independently hydrogen, halogen, substituted or unsubstituted C1-6 alkyl, –ORa, –COORa, –CORa, –N(Ra)2, –CN, or –C(=O)N(Ra)2, or two instances of R9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of Ra is independently hydrogen, substituted or unsubstituted, C1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 12, inclusive, as valency permits. 2. The compound of claim 1, wherein L1 isunsubstituted C2-4 alkylene, unsubstituted C2-4 alkenylene, unsubstituted C2-4 alkynylene, unsubstituted heteroalkylene, or unsubstituted carbocyclylene. 3. The compound of claim 1 or 2, wherein the compound is of Formula (Ia): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 4. The compound of claim 1 or 2, wherein the compound is of Formula (Ib): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 5. The compound of claim 1, wherein L1is substituted or unsubstituted heteroalkylene wherein the shortest path between the nitrogen atom (N) and Ring A comprises 1, 2, 3, or 4 atoms. 6. The compound of claim 1 or 5, wherein L1is substituted or unsubstituted heteroalkylene, wherein the shortest path between the nitrogen atom (N) and Ring A comprises 4 atoms selected from: 3 carbon atoms and 1 oxygen atom; or 2 carbon atoms and 2 oxygen atoms. 7. The compound of claim 1 or 5, wherein L1is substituted or unsubstituted heteroalkylene, wherein the shortest path between the nitrogen atom (N) and Ring A comprises 3 atoms selected from: 2 carbon atoms and 1 oxygen atom. 8. The compound of claim 1, wherein L1 is substituted or unsubstituted C2-4 alkenylene. 9. The compound of claim 1 or 8, wherein L1 is –CH=CH–. 10. The compound of claim 1, wherein L1 is substituted or unsubstituted carbocyclylene. 11. The compound of claim 1 or 10, wherein L1 is cyclopropylene. 12. The compound of any one of claims 1-11, wherein Ring A is pyridinyl, pyrazolyl, thiazolyl, imidazolyl, imidazo[1,2-a]pyrimidinyl, phenyl, thiopheneyl, cyclohexyl, or piperidinyl. 13. The compound of any one of claims 1-12, wherein Ring A is , , , 14. The compound of any one of claims 1-13, wherein Ring A is pyridinyl. 15. A compound of Formula (II): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R2 is cyclopropylmethyl or 2,2-difluoroethyl; R3 is chloro, fluoro, -CN, -CF3, or -COOCH3; L2 is a bond or substituted or unsubstituted methylene; Ring B is 3- to 13-membered carbocyclyl ring, 6- to 12-membered aryl ring, or a heterocyclyl or heteroaryl ring selected from the group consisting of: azirdinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, pyrrolyl-2,5-dione, dioxolanyl, oxathiolanyl, dithiolanyl, triazolinyl, oxadiazolinyl, thiadiazolinyl, piperidinyl, tetrahydropyranyl, dihydropyridinyl, thianyl, piperazinyl, morpholinyl, dithianyl, dioxanyl, triazinyl, azepanyl, oxepanyl, thiepanyl, azocanyl, oxecanyl, thiocanyl, indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl, tetrahydrobenzothienyl, tetrahydrobenzofuranyl, tetrahydroindolyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, decahydroisoquinolinyl, octahydrochromenyl, octahydroisochromenyl, decahydronaphthyridinyl, decahydro-1,8-naphthyridinyl, octahydropyrrolo[3,2-b]pyrrole, indolinyl, phthalimidyl, naphthalimidyl, chromanyl, chromenyl, 1H-benzo[e][1,4]diazepinyl, 1,4,5,7- tetrahydropyrano[3,4-b]pyrrolyl, 5,6-dihydro-4H-furo[3,2-b]pyrrolyl, 6,7-dihydro-5H-furo[3,2- b]pyranyl, 5,7-dihydro-4H-thieno[2,3-c]pyranyl, 4,5,6,7-tetrahydro-1H-pyrrolo[2,3-b]pyridinyl, 4,5,6,7-tetrahydrofuro[3,2-c]pyridinyl, 4,5,6,7-tetrahydrothieno[3,2-b]pyridinyl, imidazo[1,2- a]pyrimidinyl, pyrrolyl, furanyl, thiopheneyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, tetrazinyl, azepinyl, oxepinyl, thiepinyl, indolyl, isoindolyl, indazolyl, benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, purinyl, pteridinyl, cinnolinyl, quinoxalinyl, phthalazinyl, quinazolinyl, dibenzofuranyl, carbazolyl, acridinyl, phenothiazinyl, phenoxazinyl, and phenazinyl; each instance of R9 is independently hydrogen, halogen, substituted or unsubstituted C1-6 alkyl, –ORa, –COORa, –CORa, –N(Ra)2, –CN, or –C(=O)N(Ra)2, or two instances of R9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of Ra is independently hydrogen, substituted or unsubstituted, C1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. 16. The compound of claim 15, wherein the compound is of Formula (IIa): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 17. The compound of claim 15, wherein the compound is of Formula (IIb): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 18. The compound of any one of claims 15-17, wherein Ring B is phenyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, tetrahydrofuranyl, tetrahydropyranyl, thiophenyl, thiazolyl, oxazolyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, or pyridazinyl. 19. The compound of any one of claims 15-17, wherein Ring B is phenyl, tetrahydrofuranyl, thiophenyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, or pyridazinyl. 20. The compound of any one of claims 15-17, wherein Ring B is , , , 21. A compound of Formula (III): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R2 is cyclopropylmethyl or 2,2-difluoroethyl; R3 is chloro, fluoro, -CN, -CF3, or -COOCH3; L3 is a bond, substituted or unsubstituted C1-4 alkylene, substituted or unsubstituted C2-4 alkenylene, substituted or unsubstituted C2-4 alkynylene, substituted or unsubstituted heteroalkylene, or substituted or unsubstituted carbocyclylene; Ring A is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R9 is independently hydrogen, halogen, substituted or unsubstituted C1-6 alkyl, –ORa, –COORa, –CORa, –N(Ra)2, –CN, or –C(=O)N(Ra)2, or two instances of R9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of Ra is independently hydrogen, substituted or unsubstituted, C1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. 22. The compound of claim 21, wherein L3 isunsubstituted C2-4 alkylene, unsubstituted C2-4 alkenylene, unsubstituted C2-4 alkynylene, unsubstituted heteroalkylene, or unsubstituted carbocyclylene. 23. The compound of claim 21 or 22, wherein the compound is of Formula (IIIa): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 24. The compound of claim 21 or 22, wherein the compound is of Formula (IIIb): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 25. The compound of claim 21 or 22, wherein the compound is of Formula (IIIc): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 26. The compound of claim 21 or 22, wherein the compound is of Formula (IIId): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 27. The compound of claim 21, wherein L3is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 1, 2, 3, or 4 atoms. 28. The compound of claim 21 or 27, wherein L3is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 4 atoms selected from: 3 carbon atoms and 1 oxygen atom; or 2 carbon atoms and 2 oxygen atoms. 29. The compound of claim 21 or 27, wherein L3is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 3 atoms selected from: 2 carbon atoms and 1 oxygen atom. 30. The compound of claim 21 or 27, wherein L3is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 2 atoms selected from: 1 carbon atom and 1 oxygen atom. 31. The compound of claim 21 or 27, wherein L3is a heteroalkylene, wherein the heteroalkylene is –O–. 32. The compound of claim 21, wherein L3 is substituted or unsubstituted C2-4 alkenylene. 33. The compound of claim 21 or 32, wherein L3 is –CH=CH–. 34. The compound of claim 21, wherein L3 is substituted or unsubstituted carbocyclylene. 35. The compound of claim 21 or 34, wherein L3 is cyclopropylene. 36. The compound of any one of claims 21-35, wherein Ring A is phenyl, cyclohexyl, pyridinyl, pyrazolyl, imidazolyl, imidazo[1,2-a]pyridinyl, or imidazo[1,2-a]pyrimidinyl. 37. The compound of any one of claims 21-36, wherein Ring A is , , , 38. The compound of any one of claims 21-37, wherein Ring A is pyridinyl. 39. A compound of Formula (IV): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R2 is cyclopropylmethyl or 2,2-difluoroethyl; R3 is chloro, fluoro, -CN, -CF3, or -COOCH3; Ring D is 3- to 13-membered heterocyclyl or 5- to 14-membered heteroaryl ring; each instance of R9 is independently hydrogen, halogen, substituted or unsubstituted C1-6 alkyl, –ORa, –COORa, –CORa, –N(Ra)2, –CN, or –C(=O)N(Ra)2, or two instances of R9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of Ra is independently hydrogen, substituted or unsubstituted, C1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. 40. The compound of claim 39, wherein Ring D is pyrrolidinyl. 41. The compound of claim 39 or 40, wherein two instances of R9 are joined to form a 6-membered aryl or 6-membered heteroaryl ring. 42. The compound of any one of claims 39-41, wherein 43. The compound of any one of claims 39-41, wherein 44. The compound of any one of claims 1-43, wherein R2 is cyclopropylmethyl; and R3 is chloro, fluoro, or -COOCH3. 45. The compound of any one of claims 1-44, wherein R2 is cyclopropylmethyl; and R3 is chloro. 46. The compound of any one of claims 1-45, wherein R2 is 2,2-difluoroethyl; and R3 is chloro, fluoro, or -COOCH3. 47. The compound of any one of claims 1-43 and 46, wherein R2 is 2,2-difluoroethyl; and R3 is chloro. 48. The compound of any one of claims 1-15 and 21-37, wherein Ring A is pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl; and R3 is chloro. 49. The compound of any one of claims 16-20, wherein Ring B is pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl; and R3 is chloro. 50. The compound of claim 1, wherein the compound is of the formula: a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 1. The compound of claim 15, wherein the compound is of the formula: or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 52. The compound of claim 15, wherein the compound is of the formula: or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 53. The compound of claim 21, wherein the compound is of the formula: , , , , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 54. The compound of claim 39, wherein the compound is of the formula: , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 55. A compound of the formula: , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 56. The compound of any one of claims 1-55, wherein the compound is of Formula (I), (II), (III), or (IV), or salt thereof. 57. A pharmaceutical composition comprising a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof; and optionally, a pharmaceutically acceptable excipient. 58. The pharmaceutical composition of claim 57 further comprising an additional pharmaceutical agent. 59. The pharmaceutical composition of claim 58, wherein the additional pharmaceutical agent is a chemotherapeutic agent. 60. The pharmaceutical composition of claim 58, wherein the additional pharmaceutical agent is a differentiation agent. 61. A method of inhibiting nicotinamide phosphoribosyltransferase (NAMPT) in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 62. A method of treating a disease or disorder in a subject by inhibiting NAMPT in a subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 63. A method of treating a disease or disorder in a subject comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 64. The method of claim 62 or 63, wherein the disease or disorder is an inflammatory disease, proliferative disease, autoimmune disease, hematological disease, neurological disease, painful condition, metabolic disorder, infectious disease, cardiovascular disease, cerebrovascular disease, tissue repair disorder, pulmonary disease, dermatological disease, bone disease, or hormonal disease. 65. The method of claim 62 or 63, wherein the disease or disorder is a tumor, acute coronary syndrome, acute lung injury, acute respiratory distress syndrome, severe acute respiratory distress syndrome, adult respiratory distress syndrome, ventilator-induced lung injury, chronic obstructive pulmonary disease (COPD), pulmonary inflammation, Alzheimer’s disease, axon degeneration, arthritis, asthma, ataxia telangiectasia, atherogenic inflammatory disease, atherosclerosis, cachexia, colitis, ulcerative colitis (UC), Crohn’s disease (CD), inflammatory bowel disease (IBD), dermatosis, atopic dermatitis, psoriasis, diabetes, weight loss, aging, dilated cardiomyopathy, heart failure, inflammation, fibrotic diseases, glomerulonephritis, graft-versus-host disease (GCDH), Acquired Immune Deficiency Syndrome (AIDS), inflammation associated with infection, pneumonia, pneumonitis, COVID-19, a coronavirus infection, sepsis, septic shock, an intrauterine infection, polycystic ovary syndrome, preeclampsia, obesity, osteoarthritis, osteoporosis, pain, restenosis, stroke, ultra-violet induced skin damage, an atherothrombotic disease, or vascular inflammation. 66. A method of treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 67. The method of claim 66, wherein the cancer comprises cancer stem cells. 68. The method of claim 66 or 67, wherein the cancer is blood cancer, gastric cancer, colorectal cancer, gastrointestinal stromal tumor, ovarian cancer, lung cancer, liver cancer, breast cancer, pancreatic cancer, uterine cancer, testicular cancer, or prostate cancer. 69. The method of any one of claims 66-68, wherein the cancer is gastric cancer subtype GS or gastric cancer subtype CIN. 70. The method of any one of claims 66-69, wherein the cancer is colorectal cancer. 71. The method of claim 70, wherein the cancer is colorectal cancer subtype CMS2 or colorectal cancer subtype CMS4. 72. The method of claim 68, wherein the uterine cancer is endometrial cancer. 73. The method of claim 68, wherein the lung cancer is small cell lung cancer. 74. The method of claim 68, wherein the blood cancer is lymphoma. 75. The method of claim 74, wherein the lymphoma is non-Hodgkin’s lymphoma, B-cell lymphoma, large B-cell lymphoma, Burkitt’s lymphoma, Burkitt’s B-cell lymphoma, or large cell immunoblastic lymphoma. 76. The method of claim 68, wherein the blood cancer is leukemia. 77. The method of claim 76, wherein the leukemia is acute myeloid leukemia. 78. The method of claim 77, wherein the acute myeloid leukemia is undifferentiated acute myeloblastic leukemia (M0), acute myeloblastic leukemia with minimal maturation (M1), acute myeloblastic leukemia with maturation (M2), acute promyelocytic leukemia (APL) (M3), acute myelomonocytic leukemia (M4), acute myelomonocytic leukemia with eosinophilia (M4 eos), acute monocytic leukemia (M5), acute erythroid leukemia (M6), or acute megakaryoblastic leukemia (M7). 79. The method of claim 76, wherein the leukemia is acute monocytic leukemia, acute lymphocytic leukemia, or B-cell acute lymphocytic leukemia. 80. The method of claim 76, wherein the leukemia is chronic myelocytic leukemia (CML) or chronic lymphocytic leukemia. 81. The method of claim 76, wherein the leukemia is acute lymphoblastic leukemia, B-cell acute lymphoblastic leukemia, or T-cell acute lymphoblastic leukemia. 82. The method of claim 68, wherein the blood cancer is multiple myeloma. 83. The method of claim 82, wherein the multiple myeloma cancer is B-cell myeloma. 84. The method of any one of claims 66-83, wherein the subject has or is undergoing one or more additional cancer therapies. 85. The method of any one of claims 66-84, wherein the subject is in need of a regenerative medicine. 86. A method comprising contacting a cell, tissue, or biological sample with an effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 87. The method of claim 86, wherein the cell is characterized by one or more embryonic, pluripotent, or adult stem cell properties. 88. The method of claim 86 or 87, wherein the cell is a cancer stem cell, an embryonic stem cell, an induced pluripotent stem cell, a neural stem cell, a differentiated cancer cell, or an adult stem cell. 89. The method of any one of claims 86-88, wherein the step of contacting reduces one or more embryonic properties or adult stem cell properties of the cell. 90. The method of any one of claims 86-89, wherein the step of contacting is performed in vitro or ex vivo. 91. The method of any one of claims 86-90, wherein the step of contacting reduces cell viability and/or prevents cell proliferation. 92. The method of any one of claims 86-91, wherein the step of contacting kills the cell. 93. The method of any one of claims 86-92, wherein the step of contacting inhibits tumor growth. 94. The method of any one of claims 86-93, wherein the step of contacting prevents metastasis. 95. The method of any one of claims 86-94, wherein the step of contacting differentiates one or more cells. 96. The method of any one of claims 85-94, wherein the step of contacting regenerates one or more cells. 97. A method comprising killing a cell with an effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 98. The method of claim 97, wherein the cell is characterized by one or more embryonic properties or adult stem cell properties. 99. The method of claim 97 or 98, wherein the cell is a cancer stem cell, an embryonic stem cell, an induced pluripotent stem cell, a neural stem cell, an adult stem cell, or a differentiated cancer cell. 100. The method of any one of claims 97-99, wherein the cell is in vitro or ex vivo. 101. A method of treating a disease or disorder in a subject by inhibiting the production of nicotinamide adenine dinucleotides in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 102. A method of treating a disease or disorder in a subject by inhibiting the production of nicotinamide mononucleotides in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition of any one of claims 57-60. 103. A method of treating a disease or disorder in a subject by reducing inflammatory cell infiltration in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 104. A method of treating a disease or disorder in a subject by reducing cell proliferation in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 105. A method of treating a disease or disorder in a subject by reducing cellular metabolic activity in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition of any one of claims 57-60. 106. A method of treating a disease or disorder in a subject by decreasing inflammatory activity in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 107. A method of treating a disease or disorder in a subject by decreasing NAMPT signaling in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 108. A method of treating a disease or disorder in a subject by inhibiting a NAMPT pathway in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition of any one of claims 57-60. 109. A method of inhibiting production of nicotinamide adenine dinucleotides in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 110. A method of inhibiting production of nicotinamide mononucleotide in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 111. A method of inhibiting production of nicotinamide adenine dinucleotide in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 112. A method of inhibiting production of nicotinamide mononucleotide in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 113. A method of reducing inflammatory cell infiltration in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 114. A method of reducing inflammatory cell infiltration in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 115. A method of reducing cell proliferation in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 116. A method of reducing cell proliferation in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1- 56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 117. A method of reducing cellular metabolic activity in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition of any one of claims 57-60. 118. A method of reducing cellular metabolic activity in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 119. A method of decreasing inflammatory activity in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 120. A method of decreasing inflammatory activity in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 121. A method of decreasing NAMPT signaling in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 122. A method of decreasing NAMPT signaling in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1- 56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition of any one of claims 57-60. 123. A method of inhibiting a NAMPT pathway in a subject, the method comprising administering to the subject a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 124. A method of inhibiting a NAMPT pathway in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1- 56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 125. A method of inhibiting NAMPT in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 126. The method of any one of claims 111, 112, 114, 116, 118, 120, 122, 124, and 125, wherein the step of contacting is performed in vitro or ex vivo. 127. A method of treating a disease or disorder in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound of any one of claims 1- 56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60, and an additional pharmaceutical agent. 128. The method of claim 127, wherein the additional pharmaceutical agent is administered, before, concurrently with, or after the compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a composition of any one of claims 57-60. 129. The method of claim 127 or 128, wherein the disease or disorder is an inflammatory disease, proliferative disease, autoimmune disease, hematological disease, neurological disease, painful condition, metabolic disorder, infectious disease, cardiovascular disease, cerebrovascular disease, tissue repair disorder, pulmonary disease, dermatological disease, bone disease, or hormonal disease. 130. The method of claim 127 or 128, wherein the disease or disorder is a tumor, acute coronary syndrome, acute lung injury, acute respiratory distress syndrome, severe acute respiratory distress syndrome, adult respiratory distress syndrome, ventilator-induced lung injury, chronic obstructive pulmonary disease (COPD), pulmonary inflammation, Alzheimer’s disease, axon degeneration, arthritis, asthma, ataxia telangiectasia, atherogenic inflammatory disease, atherosclerosis, cachexia, colitis, ulcerative colitis (UC), Crohn’s disease (CD), inflammatory bowel disease (IBD), dermatosis, atopic dermatitis, psoriasis, diabetes, weight loss, aging, dilated cardiomyopathy, heart failure, inflammation, fibrotic diseases, glomerulonephritis, graft-versus-host disease (GCDH), Acquired Immune Deficiency Syndrome (AIDS), inflammation associated with infection, pneumonia, pneumonitis, COVID-19, a coronavirus infection, sepsis, septic shock, an intrauterine infection, polycystic ovary syndrome, preeclampsia, obesity, osteoarthritis, osteoporosis, pain, restenosis, stroke, ultra-violet induced skin damage, an atherothrombotic disease, or vascular inflammation. 131. A kit comprising : a compound of any one of claims 1-56, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof; or a composition of claim 57-60; and instructions for using the compound, pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, prodrug, or composition. |
[0217] In certain embodiments, Ring A is and L 1 is propylene. In certain embodiments, Ring A is , , , R 3 is chlo 1 ro; and L is propylene. [0218] In certain embodiments, Ring A is and R 3 is chloro. In some embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, Ring A is 2 R is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro. In certain embodiments, Ring A is , , , ; R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring A is R 2 is 2,2-difluor 3 oethyl; and R is chloro. [0219] In certain embodiments, Ring A is ; and L 1 is propylene. In certain embodiments, Ring A is ; R 3 is chloro; and L 1 is propylene. [0220] In certain embodiments, Ring A is ; and R 3 is chloro. In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro. In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 . In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro. Compounds of Formula (II) [0221] In some embodiments, the present disclosure provides compounds of Formula (II): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; L 2 is a bond or substituted or unsubstituted methylene; Ring B is 3- to 13-membered carbocyclyl ring, 6- to 12-membered aryl ring, or a heterocyclyl or heteroaryl ring selected from the group consisting of: azirdinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, pyrrolyl-2,5-dione, dioxolanyl, oxathiolanyl, dithiolanyl, triazolinyl, oxadiazolinyl, thiadiazolinyl, piperidinyl, tetrahydropyranyl, dihydropyridinyl, thianyl, piperazinyl, morpholinyl, dithianyl, dioxanyl, triazinyl, azepanyl, oxepanyl, thiepanyl, azocanyl, oxecanyl, thiocanyl, indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl, tetrahydrobenzothienyl, tetrahydrobenzofuranyl, tetrahydroindolyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, decahydroisoquinolinyl, octahydrochromenyl, octahydroisochromenyl, decahydronaphthyridinyl, decahydro-1,8-naphthyridinyl, octahydropyrrolo[3,2-b]pyrrole, indolinyl, phthalimidyl, naphthalimidyl, chromanyl, chromenyl, 1H-benzo[e][1,4]diazepinyl, 1,4,5,7- tetrahydropyrano[3,4-b]pyrrolyl, 5,6-dihydro-4H-furo[3,2-b]pyrrolyl, 6,7-dihydro-5H-furo[3,2- b]pyranyl, 5,7-dihydro-4H-thieno[2,3-c]pyranyl, 4,5,6,7-tetrahydro-1H-pyrrolo[2,3-b]pyridinyl, 4,5,6,7-tetrahydrofuro[3,2-c]pyridinyl, 4,5,6,7-tetrahydrothieno[3,2-b]pyridinyl, imidazo[1,2- a]pyrimidinyl, pyrrolyl, furanyl, thiopheneyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, tetrazinyl, azepinyl, oxepinyl, thiepinyl, indolyl, isoindolyl, indazolyl, benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, purinyl, pteridinyl, cinnolinyl, quinoxalinyl, phthalazinyl, quinazolinyl, dibenzofuranyl, carbazolyl, acridinyl, phenothiazinyl, phenoxazinyl, and phenazinyl; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R a is independently hydrogen, substituted or unsubstituted, C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. [0222] In some embodiments, the present disclosure provides compounds of Formula (II): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; L 2 is a bond or substituted or unsubstituted methylene; Ring B is 3- to 13-membered carbocyclyl ring, 6- to 12-membered aryl ring, or a heterocyclyl or heteroaryl ring selected from the group consisting of: azirdinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, pyrrolyl-2,5-dione, dioxolanyl, oxathiolanyl, dithiolanyl, triazolinyl, oxadiazolinyl, thiadiazolinyl, piperidinyl, tetrahydropyranyl, dihydropyridinyl, thianyl, piperazinyl, morpholinyl, dithianyl, dioxanyl, triazinyl, azepanyl, oxepanyl, thiepanyl, azocanyl, oxecanyl, thiocanyl, indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl, tetrahydrobenzothienyl, tetrahydrobenzofuranyl, tetrahydroindolyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, decahydroisoquinolinyl, octahydrochromenyl, octahydroisochromenyl, decahydronaphthyridinyl, decahydro-1,8-naphthyridinyl, octahydropyrrolo[3,2-b]pyrrole, indolinyl, phthalimidyl, naphthalimidyl, chromanyl, chromenyl, 1H-benzo[e][1,4]diazepinyl, 1,4,5,7- tetrahydropyrano[3,4-b]pyrrolyl, 5,6-dihydro-4H-furo[3,2-b]pyrrolyl, 6,7-dihydro-5H-furo[3,2- b]pyranyl, 5,7-dihydro-4H-thieno[2,3-c]pyranyl, 4,5,6,7-tetrahydro-1H-pyrrolo[2,3-b]pyridinyl, 4,5,6,7-tetrahydrofuro[3,2-c]pyridinyl, 4,5,6,7-tetrahydrothieno[3,2-b]pyridinyl, imidazo[1,2- a]pyrimidinyl, pyrrolyl, furanyl, thiopheneyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, tetrazinyl, azepinyl, oxepinyl, thiepinyl, indolyl, isoindolyl, indazolyl, benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, purinyl, pteridinyl, cinnolinyl, quinoxalinyl, phthalazinyl, quinazolinyl, dibenzofuranyl, carbazolyl, acridinyl, phenothiazinyl, phenoxazinyl, and phenazinyl; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R a is independently hydrogen, substituted or unsubstituted, C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. [0223] In some embodiments, L 2 is a bond. In some embodiments, the disclosure provides compounds of Formula (IIa): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0224] In some embodiments, L 2 is substituted or unsubstituted methylene. In some embodiments, L 2 is substituted methylene. In some embodiments, L is methylene substituted with methyl, halo, or two bonds on the same carbon are joined to form a ring. In some embodiments, L is , , , In some embodiments, L 2 is unsubstituted methylene. In some embodiments, the disclosure provides compounds of Formula (IIb): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0225] In some embodiments, the disclosure provides compounds of Formula (IId): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0226] In some embodiments, the disclosure provides compounds of Formula (IIe): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0227] In some embodiments, the disclosure provides compounds of Formula (IIf): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0228] In some embodiments, the disclosure provides compounds of Formula (IIg): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0229] In some embodiments, the disclosure provides compounds of Formula (IIh): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0230] In some embodiments, the disclosure provides compounds of Formula (IIi): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0231] In some embodiments, the disclosure provides compounds of Formula (IIj): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0232] In some embodiments, the disclosure provides compounds of Formula (IIk): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0233] In some embodiments, the disclosure provides compounds of Formula (IIl): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0234] In some embodiments, the disclosure provides compounds of Formula (IIm): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0235] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl. In some embodiments, R 2 is cyclopropylmethyl. In some embodiments, R 2 is 2,2-difluoroethyl. [0236] In some embodiments, R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 3 is chloro. In some embodiments, R 3 is fluoro. In some embodiments, R 3 is -COOCH 3 . In certain embodiments, R 3 is -CN. In certain embodiments, R 3 is -CF 3 . [0237] In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 . In some embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; and R 3 is chloro. In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro. In some embodiments, R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro. [0238] In some embodiments, n is an integer from 0 to 12, inclusive, as valency permits. In some embodiments, n is an integer from 0 to 6, inclusive. In some embodiments, n is an integer from 0 to 5, inclusive. In some embodiments, n is an integer from 0 to 4, inclusive. In some embodiments, n is 0. In some embodiments, n is 1. In some embodiments, n is 2. In certain embodiments, n is 3. In some embodiments, n is 4. In some embodiments, n is 5. [0239] In some embodiments, Ring B is 3- to 13-membered carbocyclyl ring, 6- to 12-membered aryl ring, or heterocyclyl ring or heteroaryl ring selected from the group consisting of: azirdinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, pyrrolyl-2,5-dione, dioxolanyl, oxathiolanyl, dithiolanyl, triazolinyl, oxadiazolinyl, thiadiazolinyl, piperidinyl, tetrahydropyranyl, dihydropyridinyl, thianyl, piperazinyl, morpholinyl, dithianyl, dioxanyl, triazinyl, azepanyl, oxepanyl, thiepanyl, azocanyl, oxecanyl, thiocanyl, indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl, tetrahydrobenzothienyl, tetrahydrobenzofuranyl, tetrahydroindolyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, decahydroisoquinolinyl, octahydrochromenyl, octahydroisochromenyl, decahydronaphthyridinyl, decahydro-1,8-naphthyridinyl, octahydropyrrolo[3,2-b]pyrrole, indolinyl, phthalimidyl, naphthalimidyl, chromanyl, chromenyl, 1H-benzo[e][1,4]diazepinyl, 1,4,5,7-tetrahydropyrano[3,4- b]pyrrolyl, 5,6-dihydro-4H-furo[3,2-b]pyrrolyl, 6,7-dihydro-5H-furo[3,2-b]pyranyl, 5,7-dihydro-4H- thieno[2,3-c]pyranyl, 4,5,6,7-tetrahydro-1H-pyrrolo[2,3-b]pyridinyl, 4,5,6,7-tetrahydrofuro[3,2- c]pyridinyl, 4,5,6,7-tetrahydrothieno[3,2-b]pyridinyl, imidazo[1,2-a]pyrimidinyl, pyrrolyl, furanyl, thiopheneyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, tetrazinyl, azepinyl, oxepinyl, thiepinyl, indolyl, isoindolyl, indazolyl, benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, purinyl, pteridinyl, cinnolinyl, quinoxalinyl, phthalazinyl, quinazolinyl, dibenzofuranyl, carbazolyl, acridinyl, phenothiazinyl, phenoxazinyl, and phenazinyl. In some embodiments, Ring B is 3- to 13-membered carbocyclyl ring. In certain embodiments, Ring B is cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, or cyclooctyl. In some embodiments, Ring B is 6- to 12-membered aryl ring. In some embodiments, Ring B is phenyl. In some embodiments, heterocyclyl ring or heteroaryl ring selected from the group consisting of: azirdinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, pyrrolyl-2,5- dione, dioxolanyl, oxathiolanyl, dithiolanyl, triazolinyl, oxadiazolinyl, thiadiazolinyl, piperidinyl, tetrahydropyranyl, dihydropyridinyl, thianyl, piperazinyl, morpholinyl, dithianyl, dioxanyl, triazinyl, azepanyl, oxepanyl, thiepanyl, azocanyl, oxecanyl, thiocanyl, indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl, tetrahydrobenzothienyl, tetrahydrobenzofuranyl, tetrahydroindolyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, decahydroisoquinolinyl, octahydrochromenyl, octahydroisochromenyl, decahydronaphthyridinyl, decahydro-1,8-naphthyridinyl, octahydropyrrolo[3,2-b]pyrrole, indolinyl, phthalimidyl, naphthalimidyl, chromanyl, chromenyl, 1H-benzo[e][1,4]diazepinyl, 1,4,5,7-tetrahydropyrano[3,4- b]pyrrolyl, 5,6-dihydro-4H-furo[3,2-b]pyrrolyl, 6,7-dihydro-5H-furo[3,2-b]pyranyl, 5,7-dihydro-4H- thieno[2,3-c]pyranyl, 4,5,6,7-tetrahydro-1H-pyrrolo[2,3-b]pyridinyl, 4,5,6,7-tetrahydrofuro[3,2- c]pyridinyl, 4,5,6,7-tetrahydrothieno[3,2-b]pyridinyl, imidazo[1,2-a]pyrimidinyl, pyrrolyl, furanyl, thiopheneyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, tetrazinyl, azepinyl, oxepinyl, thiepinyl, indolyl, isoindolyl, indazolyl, benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, purinyl, pteridinyl, cinnolinyl, quinoxalinyl, phthalazinyl, quinazolinyl, dibenzofuranyl, carbazolyl, acridinyl, phenothiazinyl, phenoxazinyl, and phenazinyl. In some embodiments, Ring B is phenyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, tetrahydrofuranyl, tetrahydropyranyl, thiophenyl, thiazolyl, oxazolyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, pyridazinyl, In some embodiments, Ring B is phenyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, tetrahydrofuranyl, tetrahydropyranyl, thiophenyl, thiazolyl, oxazolyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments, Ring B is phenyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, tetrahydrofuranyl, thiophenyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In certain embodiments, Ring B is phenyl, tetrahydrofuranyl, thiophenyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, pyridazinyl, , , , In certain embodiments, Ring B is In certain embodiments, Ring B is phenyl, tetrahydrofuranyl, thiophenyl, imidazolyl, pyrazolyl, pyrrolyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments, Ring B is . [0240] In some embodiments, each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –C(=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring. In certain embodiments, each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , – N(R a ) 2 , –CN, or –C(=O)N(R a ) 2 . Each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , or –N(R a ) 2 . In certain embodiments, at least one instance of R 9 is hydrogen. In certain embodiments, each instance of R 9 is hydrogen. In certain embodiments, at least one instance of R 9 is not hydrogen. In certain embodiments, no instance of R 9 is hydrogen. In certain embodiments, at least one instance of R 9 is halogen. In certain embodiments, at least one instance of R 9 is F. In certain embodiments, at least one instance of R 9 is Cl. In certain embodiments, at least one instance of R 9 is substituted or unsubstituted C 1-6 alkyl (e.g., unsubstituted C 1-6 alkyl). In certain embodiments, at least one instance of R 9 is Me. In certain embodiments, at least one instance of R 9 is Et. In certain embodiments, at least one instance of R 9 is Pr or Bu. In certain embodiments, at least one instance of R 9 is fluorinated C 1-6 alkyl (e.g., fluorinated methyl, e.g., –CF 3 ). In certain embodiments, at least one instance of R 9 is –OR a . In certain embodiments, at least one instance of R 9 is –OH. In certain embodiments, at least one instance of R 9 is –O(substituted or unsubstituted C 1-6 alkyl). In certain embodiments, at least one instance of R 9 is –OMe. In certain embodiments, at least one instance of R 9 is –N(R a ) 2 . In certain embodiments, at least one instance of R 9 is –NH 2 . In certain embodiments, at least one instance of R 9 is –NHR a (e.g., –NH(substituted or unsubstituted C 1-6 alkyl), e.g., –NHMe). In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is a substituted C 1-6 alkyl. In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is a C 1-6 alkyl substituted with -NH 2 , -NHMe, or- NMe 2 . In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is an unsubstituted C 1-6 alkyl. In certain embodiments, at least one instance of R 9 is –N(substituted or unsubstituted C 1-6 alkyl) 2 . In certain embodiments, at least one instance of R 9 is –N(Me) 2 . In certain embodiments, at least one instance of R 9 is –CN. In certain embodiments, at least one instance of R 9 is halogen, substituted or unsubstituted C 1-6 alkyl, or –OR a . In certain embodiments, more than one instance of R 9 is C 1-6 alkyl (e.g., two instances of R 9 are methyl). In certain embodiments, more than one instance of R 9 is halogen (e.g., two instances of R 9 are fluoro). In some embodiments, one instance of R 9 is C 1-6 alkyl, and a second instance of R 9 is halogen. In some embodiments, one instance of R 9 is methyl, and a second instance of R 9 is fluoro. [0241] In some embodiments, two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring. In some embodiments, two instances of R 9 are joined to form a 3- to 6-membered carbocyclyl, 3- to 6-membered heterocyclyl, 6-membered aryl ring, or 5- to 6-membered heteroaryl ring. In certain embodiments, two instances of R 9 are joined to form a 5-membered heteroaryl ring. In certain embodiments, two instances of R 9 are joined to form a 5-membered heterocyclyl ring. [0242] In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 . In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and Ring B is pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. [0243] In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and Ring B is pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and Ring B is pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. [0244] In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 2 is a bond. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 2 is a bond. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and L 2 is -CH 2 -. [0245] In some embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 . In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and Ring B pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. In some embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is 2,2- difluoroethyl; and R 3 is chloro. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and Ring B pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and Ring B is pyrazolyl, imidazolyl, or imidazo[1,2- a]pyrimidinyl. [0246] In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 2 is a bond. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 2 is a bond. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and L 2 is -CH 2 -. [0247] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro; and L 2 is a bond. In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro; and L 2 is - CH 2 -. [0248] In some embodiments, Ring B is imidazo[1,2-a]pyrimidinyl; and R 3 is chloro. In some embodiments, Ring B is imidazo[1,2-a]pyrimidinyl; and L 2 is -CH 2 -. In some embodiments, Ring B is imidazo[1,2-a]pyrimidinyl; and L 2 is a bond. [0249] In certain embodiments, Ring B is and L 2 is a bond. In some embodiments, Ring B is , , , R 3 is chloro; and L 2 is a bond. [0250] In certain embodiments, Ring B is , , , ; and L 2 is methylene. In certain embodiments, Ring B is , , , or R 3 is chloro; a 2 nd L is methylene. [0251] In certain embodiments, Ring B is ; and R 3 is chloro. In some embodiments, Ring B is ; R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, Ring B is 2 R is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring B is , , , ; R 2 is cyclopropylmethyl; and R 3 is chloro. In certain embodiments, Ring B is ; R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In certain embodiments, Ring B is R 2 is 2,2-dif 3 luoroethyl; and R is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring B is , , , or ; R 2 is 2,2-difluoroethyl; and R 3 is chloro. Compounds of Formula (III) [0252] In some embodiments, the disclosure provides compounds of Formula (III): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; L 3 is a bond, substituted or unsubstituted C 1-4 alkylene, substituted or unsubstituted C 2-4 alkenylene, substituted or unsubstituted C 2-4 alkynylene, substituted or unsubstituted heteroalkylene, or substituted or unsubstituted carbocyclylene; Ring A is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R a is independently hydrogen, substituted or unsubstituted, C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. [0253] In some embodiments, the disclosure provides compounds of Formula (III): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; L 3 is a bond, substituted or unsubstituted C 1-4 alkylene, substituted or unsubstituted C 2-4 alkenylene, substituted or unsubstituted C 2-4 alkynylene, substituted or unsubstituted heteroalkylene, or substituted or unsubstituted carbocyclylene; Ring A is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R a is independently hydrogen, substituted or unsubstituted, C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. [0254] In some embodiments, L 3 is a bond. In some embodiments, the disclosure provides compounds of Formula (IIIa): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0255] In some embodiments, L 3 is substituted or unsubstituted C1-4 alkylene. In some embodiments, L 3 issubstituted C1-4 alkylene. In some embodiments, L 3 is unsubstituted C1-4 alkylene. In certain embodiments, L 3 is methylene. In certain embodiments, L 3 is ethylene. In some embodiments, L 3 is propylene. In certain embodiments, L 3 is butylene. In some embodiments, L 3 is methylene substituted with halo or methyl. In some embodiments, L 3 is , , , . [0256] In some embodiments, the disclosure provides compounds of Formula (IIIb): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0257] In some embodiments, the disclosure provides compounds of Formula (IIIc): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0258] In some embodiments, the disclosure provides compounds of Formula (IIId): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0259] In some embodiments, the disclosure provides compounds of Formula (IIIe): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0260] In some embodiments, the disclosure provides compounds of Formula (IIIf): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0261] In some embodiments, the disclosure provides compounds of Formula (IIIg): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0262] In some embodiments, the disclosure provides compounds of Formula (IIIh): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0263] In some embodiments, the disclosure provides compounds of Formula (IIIi): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0264] In some embodiments, the disclosure provides compounds of Formula (IIIj): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0265] In some embodiments, the disclosure provides compounds of Formula (IIIk): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0266] In some embodiments, the disclosure provides compounds of Formula (IIIl): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0267] In some embodiments, the disclosure provides compounds of Formula (IIIm): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0268] In some embodiments, the disclosure provides compounds of Formula (IIIn): , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0269] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl. In some embodiments, R 2 is cyclopropylmethyl. In some embodiments, R 2 is 2,2-difluoroethyl. [0270] In some embodiments, R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 3 is chloro. In some embodiments, R 3 is fluoro. In some embodiments, R 3 is -COOCH 3 . In some embodiments, R 3 is -CN. In certain embodiments, R 3 is -CF 3 . [0271] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; and R 3 is chloro. In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro. In some embodiments, R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro. [0272] In some embodiments, L 3 is substituted or unsubstituted C2-4 alkenylene. In certain embodiments, L 3 is C2 alkenylene. In some embodiments, L 3 is –CH=CH–. In certain embodiments, L 3 is C3 alkenylene. In certain embodiments, L 3 is –CH=CHCH 2 –. In certain embodiments, L 3 is –CH 2 CH=CH–. In certain embodiments, L 3 is C4 alkenylene. In certain embodiments, L 3 is –CH=CHCH 2 CH 2 –. In certain embodiments, L 3 is –CH 2 CH 2 CH=CH–. In certain embodiments, L 3 is –CH 2 CH=CHCH 2 –. In certain embodiments, L 3 is –CH 2 CH 2 CH=CH–. [0273] In certain embodiments, L 3 is C 2 alkynylene. In certain embodiments, L 3 is C 3 alkynylene. In certain embodiments, L 3 is C 4 alkynylene. [0274] In some embodiments, L 3 is heteroalkylene. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene wherein the shortest path between the carbonyl moiety and Ring A comprises 1, 2, 3, or 4 atoms. In some embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 4 atoms. In some embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 4 atoms selected from: 3 carbon atoms and 1 oxygen atom; or 2 carbon atoms and 2 oxygen atoms. In some embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 3 carbon atoms and 1 oxygen atom. In some embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 2 carbon atoms and 2 oxygen atoms. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 3 atoms. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 3 atoms selected from: 2 carbon atoms and 1 oxygen atom. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 2 carbon atoms and 1 oxygen atom. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 1 carbon atom and 2 oxygen atoms. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 2 atoms. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 1 carbon atom and 1 oxygen atom. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 1 atom. In certain embodiments, L 3 is substituted or unsubstituted heteroalkylene, wherein the shortest path between the carbonyl moiety and Ring A comprises 1 oxygen atom. In certain embodiments, L 3 is –O–. In certain embodiments, L 3 is –OCH 2 –. In certain embodiments, L 3 is –CH 2 O–. In certain embodiments, L 3 is –OCH 2 CH 2 –. In certain embodiments, L 3 is –CH 2 OCH 2 –. In certain embodiments, L 3 is –CH 2 CH 2 O–. In certain embodiments, L 3 is –CH 2 CH 2 CH 2 O–. In certain embodiments, L 3 is –CH 2 CH 2 OCH 2 –. In certain embodiments, L 3 is –CH 2 OCH 2 CH 2 –. In certain embodiments, L 3 is –OCH 2 CH 2 CH 2 –. [0275] In some embodiments, L 3 is substituted or unsubstituted carbocyclylene. In certain embodiments, L 3 is cyclopropylene. [0276] In some embodiments, n is an integer from 0 to 12, inclusive, as valency permits. In some embodiments, n is an integer from 0 to 6, inclusive. In some embodiments, n is an integer from 0 to 5, inclusive. In some embodiments, n is an integer from 0 to 4, inclusive. In some embodiments, n is 0. In some embodiments, n is 1. In some embodiments, n is 2. In certain embodiments, n is 3. In some embodiments, n is 4. In some embodiments, n is 5. [0277] In some embodiments, Ring A is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring. In some embodiments, Ring A is 3- to 6-membered carbocyclyl, 3- to 6-membered heterocyclyl, 6-membered aryl , or 5- to 9-membered heteroaryl ring. In certain embodiments, Ring A is phenyl, cyclohexyl, pyridinyl, pyrazolyl, imidazolyl, imidazo[1,2-a]pyridinyl, or imidazo[1,2-a]pyrimidinyl. In some embodiments, Ring A is pyridinyl, pyrazolyl, imidazolyl, imidazo[1,2-a]pyridinyl, or imidazo[1,2-a]pyrimidinyl. In some embodiments, Ring A is In some embodiments, Ring A is pyridinyl. [0278] In certain embodiments, Ring A is: . In certain embodiments, Ring A is: [0279] In certain embodiments, Ring A is: In certain embodiments, Ring A is: . In certain embodiments, Ring A is: In certain embodiments, Ring A is: . certain embodiments, Ring A is: [0280] In some embodiments, each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –C(=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring. In certain embodiments, each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , – N(R a ) 2 , –CN, or –C(=O)N(R a ) 2 . Each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , or –N(R a ) 2 . In certain embodiments, at least one instance of R 9 is hydrogen. In certain embodiments, each instance of R 9 is hydrogen. In certain embodiments, at least one instance of R 9 is not hydrogen. In certain embodiments, no instance of R 9 is hydrogen. In certain embodiments, at least one instance of R 9 is halogen. In certain embodiments, at least one instance of R 9 is F. In certain embodiments, at least one instance of R 9 is Cl. In certain embodiments, at least one instance of R 9 is substituted or unsubstituted C 1-6 alkyl (e.g., unsubstituted C 1-6 alkyl). In certain embodiments, at least one instance of R 9 is Me. In certain embodiments, at least one instance of R 9 is Et. In certain embodiments, at least one instance of R 9 is Pr or Bu. In certain embodiments, at least one instance of R 9 is fluorinated C 1-6 alkyl (e.g., fluorinated methyl, e.g., –CF 3 ). In certain embodiments, at least one instance of R 9 is –OR a . In certain embodiments, at least one instance of R 9 is –OH. In certain embodiments, at least one instance of R 9 is –O(substituted or unsubstituted C 1-6 alkyl). In certain embodiments, at least one instance of R 9 is –OMe. In certain embodiments, at least one instance of R 9 is –N(R a ) 2 . In certain embodiments, at least one instance of R 9 is –NH 2 . In certain embodiments, at least one instance of R 9 is –NHR a (e.g., –NH(substituted or unsubstituted C 1-6 alkyl), e.g., –NHMe). In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is a substituted C 1-6 alkyl. In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is a C 1-6 alkyl substituted with -NH 2 , -NHMe, or- NMe 2 . In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is an unsubstituted C 1-6 alkyl. In certain embodiments, at least one instance of R 9 is –N(substituted or unsubstituted C 1-6 alkyl) 2 . In certain embodiments, at least one instance of R 9 is –N(Me) 2 . In certain embodiments, at least one instance of R 9 is –CN. In certain embodiments, at least one instance of R 9 is halogen, substituted or unsubstituted C 1-6 alkyl, or –OR a . In certain embodiments, more than one instance of R 9 is C 1-6 alkyl (e.g., two instances of R 9 are methyl). In certain embodiments, more than one instance of R 9 is halogen (e.g., two instances of R 9 are fluoro). In some embodiments, one instance of R 9 is C 1-6 alkyl, and a second instance of R 9 is halogen. In some embodiments, one instance of R 9 is methyl, and a second instance of R 9 is fluoro. [0281] In some embodiments, two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring. In certain embodiments, two instances of R 9 are joined to form a phenyl ring. In certain embodiments, two instances of R 9 are joined to form a 6-membered heteroaryl ring. In some embodiments, two instances of R 9 are joined to form a pyridinyl ring. In certain embodiments, two instances of R 9 are joined to form a 5-membered heteroaryl ring. In certain embodiments, two instances of R 9 are joined to form a 5-membered heterocyclyl ring. In some embodiments, two instances of R 9 are joined to form pyrrolyl, imidazolyl, pyrazolyl, furanyl, oxazolyl, isoxazolyl, thiophenyl, thiazolyl, isothiazolyl, triazolyl, pyrrolinyl, pyrazolinyl, or imidazolinyl. In certain embodiments, two instances of R 9 are joined to form a 5-membered heteroaryl or heterocyclyl ring, and when viewed together with Ring A to which the R 9 groups are attached, the group is represented by: , , , , , [0282] In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In certain embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and Ring A is pyridinyl. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and Ring A is pyridinyl. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and Ring A is pyridinyl. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and Ring A is pyridinyl. [0283] In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 ; and L 3 is propylene. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, - CN, -CF 3 , or -COOCH 3 ; and L 3 is ethylene. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 3 is methylene. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 3 is a bond. [0284] In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is propylene. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and L 3 is propylene. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is ethylene. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and L 3 is ethylene. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is methylene. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and L 3 is methylene. In some embodiments, R 2 is cyclopropylmethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is a bond. In certain embodiments, R 2 is cyclopropylmethyl; R 3 is chloro; and L 3 is a bond. [0285] In some embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 . In some embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro. In some embodiments, R 2 is 2,2- difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and Ring A pyridinyl, pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and Ring A pyridinyl, pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and Ring A is pyridinyl. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and Ring A pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and Ring A pyrazolyl, imidazolyl, or imidazo[1,2-a]pyrimidinyl. [0286] In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 3 is propylene. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 3 is ethylene. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 3 is methylene. In some embodiments, R 2 is 2,2- difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; and L 3 is a bond. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is propylene. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and L 3 is propylene. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is ethylene. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and L 3 is ethylene. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; and L 3 is methylene. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and L 3 is methylene. In some embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro, fluoro, or - COOCH 3 ; and L 3 is a bond. In certain embodiments, R 2 is 2,2-difluoroethyl; R 3 is chloro; and L 3 is a bond. [0287] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro; and L 3 is propylene. In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro; and L 3 is a bond. In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro; and L 3 is methylene. In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro; and L 3 is ethylene. [0288] In some embodiments, Ring A is pyridinyl; and R 3 is chloro. In some embodiments, Ring A is pyridinyl; and L 3 is propylene. In some embodiments, Ring A is pyridinyl; and L 3 is ethylene. In some embodiments, Ring A is pyridinyl; and L 3 is methylene. In some embodiments, Ring A is pyridinyl; and L 3 is a bond. [0289] In certain embodiments, Ring A is ; and L 3 is propylene. In certain embodiments, Ring A is ; R 3 is chloro; and L 3 is propylene. In certain embodiments, Ring A is ; and L 3 is ethylene. In certain embodiments, Ring A is ; R 3 is chloro; and L 3 is ethylene. In certain embodiments, Ring A is ; and L 3 is methylene. In certain embodiments, Ring A is ; R 3 is chloro; and L 3 is methylene. In certain embodiments, Ring A is ; and L 3 is a bond. In certain embodiments, Ring A is ; R 3 is chloro; and L 3 is a bond. [0290] In certain embodiments, Ring A is ; and R 3 is chloro. In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is cyclopropylmethyl; and R 3 is chloro. In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or - COOCH 3 . In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, Ring A is ; R 2 is 2,2-difluoroethyl; and R 3 is chloro. Compounds of Formula (IV) [0291] In some embodiments, the disclosure provides compounds of Formula (IV): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 ; Ring D is 3- to 13-membered heterocyclyl or 5- to 14-membered heteroaryl ring; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted, C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3-to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R a is independently hydrogen, substituted or unsubstituted, C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. [0292] In some embodiments, the disclosure provides compounds of Formula (IV): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein: R 2 is cyclopropylmethyl or 2,2-difluoroethyl; R 3 is chloro, fluoro, or -COOCH 3 ; Ring D is 3- to 13-membered heterocyclyl or 5- to 14-membered heteroaryl ring; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted, C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3-to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; each instance of R a is independently hydrogen, substituted or unsubstituted, C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and n is an integer from 0 to 13, inclusive, as valency permits. [0293] In some embodiments, the disclosure provides compounds of Formula (IVa): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0294] In some embodiments, the disclosure provides compounds of Formula (IVb): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0295] In some embodiments, the disclosure provides compounds of Formula (IVc): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0296] In some embodiments, the disclosure provides compounds of Formula (IVd): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0297] In some embodiments, the disclosure provides compounds of Formula (IVe): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0298] In some embodiments, the disclosure provides compounds of Formula (IVf): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0299] In some embodiments, the disclosure provides compounds of Formula (IVg): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0300] In some embodiments, the disclosure provides compounds of Formula (IVh): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0301] In some embodiments, the disclosure provides compounds of Formula (IVi): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0302] In some embodiments, the disclosure provides compounds of Formula (IVj): or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0303] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl. In some embodiments, R 2 is cyclopropylmethyl. In some embodiments, R 2 is 2,2-difluoroethyl. [0304] In some embodiments, R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 .In some embodiments, R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 3 is chloro. In some embodiments, R 3 is fluoro. In some embodiments, R 3 is -COOCH 3 . In some embodiments, R 3 is -CN. In some embodiments, R 3 is -CF 3 . [0305] In certain embodiments, R 2 is cyclopropylmethyl or 2,2-difluoroethyl; and R 3 is chloro. In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 .In some embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is cyclopropylmethyl; and R 3 is chloro. In some embodiments, R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2 is 2,2- difluoroethyl; and R 3 is chloro, fluoro, or -COOCH 3 . In certain embodiments, R 2 is 2,2-difluoroethyl; and R 3 is chloro. [0306] In some embodiments, Ring D is 3- to 13-membered heterocyclyl or 5- to 14-membered heteroaryl ring. In some embodiments, Ring D is a 3- to 13-membered heterocyclyl. In some embodiments, Ring D is a 4- to 6-membered heterocyclyl. In some embodiments, Ring D is azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl. In some embodiments, Ring D is pyrrolidinyl. [0307] In some embodiments, n is an integer from 0 to 12, inclusive, as valency permits. In some embodiments, n is an integer from 0 to 6, inclusive. In some embodiments, n is an integer from 0 to 5, inclusive. In some embodiments, n is an integer from 0 to 4, inclusive. In some embodiments, n is 0. In some embodiments, n is 1. In some embodiments, n is 2. In certain embodiments, n is 3. In some embodiments, n is 4. In some embodiments, n is 5. [0308] In some embodiments, each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –C(=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring. In certain embodiments, each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , – N(R a ) 2 , –CN, or –C(=O)N(R a ) 2 . Each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , or –N(R a ) 2 . In certain embodiments, at least one instance of R 9 is hydrogen. In certain embodiments, each instance of R 9 is hydrogen. In certain embodiments, at least one instance of R 9 is not hydrogen. In certain embodiments, no instance of R 9 is hydrogen. In certain embodiments, at least one instance of R 9 is halogen. In certain embodiments, at least one instance of R 9 is F. In certain embodiments, at least one instance of R 9 is Cl. In certain embodiments, at least one instance of R 9 is substituted or unsubstituted C 1-6 alkyl (e.g., unsubstituted C 1-6 alkyl). In certain embodiments, at least one instance of R 9 is Me. In certain embodiments, at least one instance of R 9 is Et. In certain embodiments, at least one instance of R 9 is Pr or Bu. In certain embodiments, at least one instance of R 9 is fluorinated C 1-6 alkyl (e.g., fluorinated methyl, e.g., –CF 3 ). In certain embodiments, at least one instance of R 9 is –OR a . In certain embodiments, at least one instance of R 9 is –OH. In certain embodiments, at least one instance of R 9 is –O(substituted or unsubstituted C 1-6 alkyl). In certain embodiments, at least one instance of R 9 is –OMe. In certain embodiments, at least one instance of R 9 is –N(R a ) 2 . In certain embodiments, at least one instance of R 9 is –NH 2 . In certain embodiments, at least one instance of R 9 is –NHR a (e.g., –NH(substituted or unsubstituted C 1-6 alkyl), e.g., –NHMe). In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is a substituted C 1-6 alkyl. In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is a C 1-6 alkyl substituted with -NH 2 , -NHMe, or- NMe 2 . In certain embodiments, at least one instance of R 9 is –NHR a , wherein R a is an unsubstituted C 1-6 alkyl. In certain embodiments, at least one instance of R 9 is –N(substituted or unsubstituted C 1-6 alkyl) 2 . In certain embodiments, at least one instance of R 9 is –N(Me) 2 . In certain embodiments, at least one instance of R 9 is –CN. In certain embodiments, at least one instance of R 9 is halogen, substituted or unsubstituted C 1-6 alkyl, or –OR a . In certain embodiments, more than one instance of R 9 is C 1-6 alkyl (e.g., two instances of R 9 are methyl). In certain embodiments, more than one instance of R 9 is halogen (e.g., two instances of R 9 are fluoro). In some embodiments, one instance of R 9 is C 1-6 alkyl, and a second instance of R 9 is halogen. In some embodiments, one instance of R 9 is methyl, and a second instance of R 9 is fluoro. [0309] In some embodiments, two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring. In some embodiments, two instances of R 9 are joined to form a 3- to 6-membered carbocyclyl, 3- to 6-membered heterocyclyl, 6-membered aryl ring, or 5- to 6-membered heteroaryl ring. In certain embodiments, two instances of R 9 are joined to form a phenyl ring. In certain embodiments, two instances of R 9 are joined to form a 6-membered heteroaryl ring. In some embodiments, two instances of R 9 are joined to form a pyridinyl ring. In certain embodiments, two instances of R 9 are joined to form a 5-membered heteroaryl ring. In certain embodiments, two instances of R 9 are joined to form a 5-membered heterocyclyl ring. In some embodiments, two instances of R 9 are joined to form pyrrolyl, imidazolyl, pyrazolyl, furanyl, oxazolyl, isoxazolyl, thiophenyl, thiazolyl, isothiazolyl, triazolyl, pyrrolinyl, pyrazolinyl, or imidazolinyl. [0310] In some embodiments, In some embodiments,
Compound Species
[0311] In certain embodiments, a compound of Formula (I) is of the formula:
or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0312] In certain embodiments, a compound of Formula (I) is of the formula:
or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0313] In some embodiments, the compound of Formula (I) is of the formula:
, a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0314] In some embodiments, the compound of Formula (I) is of the formula:
, , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0315] In certain embodiments, a compound of Formula (II) is of the formula:
or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0316] In certain embodiments, a compound of Formula (II) is of the formula:
,
or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0317] In certain embodiments, a compound of Formula (II) is of the formula:
, or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0318] In certain embodiments, a compound of Formula (II) is of the formula:
or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0319] In certain embodiments, a compound of Formula (II) is of the formula:
a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0320] In certain embodiments, a compound of Formula (II) is of the formula: or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0321] In certain embodiments, a compound of Formula (II) is of the formula: or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0322] In certain embodiments, a compound of Formula (III) is of the formula: ,
T2289, or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0323] In some embodiments, a compound of Formula (III) is of the formula:
, ,
, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0324] In some embodiments, a compound of Formula (III) is of the formula: ,
,
or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0325] In some embodiments, a compound of Formula (III) is of the formula:
or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0326] In certain embodiments, a compound of Formula (IV) is of the formula: , or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0327] In certain embodiments, a compound of Formula (IV) is of the formula: pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0328] In certain embodiments, a compound of Formula (IV) is of the formula: or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0329] In certain embodiments, a compound of Formula (IV) is of the formula: , or a pharmaceutically acceptable salt, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0330] A compound of the formula:
, , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0331] A compound of the formula: , , , or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0332] In certain embodiments, also provided herein are compounds of the formula: pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. Compositions and Kits [0333] The present disclosure provides compositions (e.g., pharmaceutical compositions) comprising a compound of the disclosure (e.g., a compound of Formula (I), (II), (III), or (IV) or any compound described herein), and an excipient (e.g., pharmaceutically acceptable excipient). In certain embodiments, the composition is a pharmaceutical composition. In certain embodiments, the excipient is a pharmaceutically acceptable excipient. [0334] The present disclosure also provides compositions further comprising an additional pharmaceutical agent. [0335] The disclosure also provides compositions (e.g., pharmaceutical compositions) comprising a compound of the disclosure (e.g., a compound of Formula (I), (II), (III), or (IV) or any compound described herein), and one pharmaceutical agent. The disclosure also provides compositions (e.g., pharmaceutical compositions) comprising a compound of the disclosure (e.g., a compound of Formula (I), (II), (III), or (IV) or any compound described herein), and two pharmaceutical agents. The disclosure also provides compositions (e.g., pharmaceutical compositions) comprising a compound of the disclosure (e.g., a compound of Formula (I), (II), (III), or (IV) or any compound described herein), and three pharmaceutical agents. [0336] Compositions described herein can be prepared by any method known in the art. In general, such preparatory methods include bringing a compound of the disclosure described herein into association with an excipient and may include one or more agents or accessory ingredients, and then, if necessary and/or desirable, shaping, and/or packaging the product into a desired single- or multi- dose unit. In certain embodiments, the agent is a pharmaceutical agent. [0337] In certain embodiments, the compound of the disclosure is in the form of a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug. [0338] Compositions can be prepared, packaged, and/or sold in bulk, as a single unit dose, and/or as a plurality of single unit doses. A “unit dose” is a discrete amount of the composition comprising a predetermined amount of the agent. The amount of the agent is generally equal to the dosage of the agent which would be administered to a subject and/or a convenient fraction of such a dosage, such as one-half or one-third of such a dosage. [0339] Relative amounts of the compound of the disclosure, excipient, agent, and/or any additional ingredients in a composition described herein will vary, depending upon the identity, size, and/or condition of the subject treated and further depending upon the route by which the composition is to be administered. The composition may comprise between 0.1% and 100% (w/w) agent. [0340] Excipients and accessory ingredients used in the manufacture of provided compositions include inert diluents, dispersing and/or granulating agents, surface active agents and/or emulsifiers, disintegrating agents, binding agents, preservatives, buffering agents, lubricating agents, and/or oils. Excipients and accessory ingredients, such as cocoa butter, PEGylated lipids, phospholipids, suppository waxes, coloring agents, coating agents, sweetening, flavoring, and perfuming agents, may also be present in the composition. [0341] Exemplary diluents include calcium carbonate, sodium carbonate, calcium phosphate, dicalcium phosphate, calcium sulfate, calcium hydrogen phosphate, sodium phosphate lactose, sucrose, cellulose, microcrystalline cellulose, kaolin, mannitol, sorbitol, inositol, sodium chloride, dry starch, cornstarch, powdered sugar, and mixtures thereof. [0342] Exemplary granulating and/or dispersing agents include potato starch, corn starch, tapioca starch, sodium starch glycolate, clays, alginic acid, guar gum, citrus pulp, agar, bentonite, cellulose, and wood products, natural sponge, cation-exchange resins, calcium carbonate, silicates, sodium carbonate, cross-linked poly(vinyl-pyrrolidone) (crospovidone), sodium carboxymethyl starch (sodium starch glycolate), carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose (croscarmellose), methylcellulose, pregelatinized starch (starch 1500), microcrystalline starch, water insoluble starch, calcium carboxymethyl cellulose, magnesium aluminum silicate (Veegum), sodium lauryl sulfate, quaternary ammonium compounds, and mixtures thereof. [0343] Exemplary surface active agents and/or emulsifiers include natural emulsifiers (e.g., acacia, agar, alginic acid, sodium alginate, tragacanth, chondrux, xanthan, pectin, gelatin, egg yolk, casein, wool fat, cholesterol, wax, and lecithin), colloidal clays (e.g., bentonite (aluminum silicate) and Veegum (magnesium aluminum silicate)), long chain amino acid derivatives, high molecular weight alcohols (e.g., stearyl alcohol, cetyl alcohol, oleyl alcohol, triacetin monostearate, ethylene glycol distearate, glyceryl monostearate, and propylene glycol monostearate, polyvinyl alcohol), carbomers (e.g., carboxy polymethylene, polyacrylic acid, acrylic acid polymer, and carboxyvinyl polymer), carrageenan, cellulosic derivatives (e.g., carboxymethylcellulose sodium, powdered cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, methylcellulose), sorbitan fatty acid esters (e.g., polyoxyethylene sorbitan monolaurate (Tween ® 20), polyoxyethylene sorbitan monostearate (Tween ® 60), polyoxyethylene sorbitan monooleate (Tween ® 80), sorbitan monopalmitate (Span ® 40), sorbitan monostearate (Span ® 60), sorbitan tristearate (Span ® 65), glyceryl monooleate, sorbitan monooleate (Span ® 80), polyoxyethylene esters (e.g., polyoxyethylene monostearate (Myrj ® 45), polyoxyethylene hydrogenated castor oil, polyethoxylated castor oil, polyoxymethylene stearate, and Solutol ® ), sucrose fatty acid esters, polyethylene glycol fatty acid esters (e.g., Cremophor ® ), polyoxyethylene ethers, (e.g., polyoxyethylene lauryl ether (Brij ® 30)), poly(vinyl-pyrrolidone), diethylene glycol monolaurate, triethanolamine oleate, sodium oleate, potassium oleate, ethyl oleate, oleic acid, ethyl laurate, sodium lauryl sulfate, Pluronic ® F-68, poloxamer P-188, cetrimonium bromide, cetylpyridinium chloride, benzalkonium chloride, docusate sodium, and/or mixtures thereof. [0344] Exemplary binding agents include starch (e.g., cornstarch and starch paste), gelatin, sugars (e.g., sucrose, glucose, dextrose, dextrin, molasses, lactose, lactitol, mannitol, etc.), natural and synthetic gums (e.g., acacia, sodium alginate, extract of Irish moss, panwar gum, ghatti gum, mucilage of isapol husks, carboxymethylcellulose, methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, microcrystalline cellulose, cellulose acetate, poly(vinyl-pyrrolidone), magnesium aluminum silicate (Veegum ® ), and larch arabogalactan), alginates, polyethylene oxide, polyethylene glycol, inorganic calcium salts, silicic acid, polymethacrylates, waxes, water, alcohol, and/or mixtures thereof. [0345] Exemplary preservatives include antioxidants, chelating agents, antimicrobial preservatives, antifungal preservatives, antiprotozoan preservatives, alcohol preservatives, acidic preservatives, and other preservatives. In certain embodiments, the preservative is an antioxidant. In other embodiments, the preservative is a chelating agent. [0346] Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, monothioglycerol, potassium metabisulfite, propionic acid, propyl gallate, sodium ascorbate, sodium bisulfite, sodium metabisulfite, and sodium sulfite. [0347] Exemplary chelating agents include ethylenediaminetetraacetic acid (EDTA) and salts and hydrates thereof (e.g., sodium edetate, disodium edetate, trisodium edetate, calcium disodium edetate, dipotassium edetate, and the like), citric acid and salts and hydrates thereof (e.g., citric acid monohydrate), fumaric acid and salts and hydrates thereof, malic acid and salts and hydrates thereof, phosphoric acid and salts and hydrates thereof, and tartaric acid and salts and hydrates thereof. Exemplary antimicrobial preservatives include benzalkonium chloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide, cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate, propylene glycol, and thimerosal. [0348] Exemplary antifungal preservatives include butyl paraben, methyl paraben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoic acid, potassium benzoate, potassium sorbate, sodium benzoate, sodium propionate, and sorbic acid. [0349] Exemplary alcohol preservatives include ethanol, polyethylene glycol, phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate, and phenylethyl alcohol. [0350] Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E, beta-carotene, citric acid, acetic acid, dehydroacetic acid, ascorbic acid, sorbic acid, and phytic acid. [0351] Other preservatives include tocopherol, tocopherol acetate, deteroxime mesylate, cetrimide, butylated hydroxyanisol (BHA), butylated hydroxytoluened (BHT), ethylenediamine, sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES), sodium bisulfite, sodium metabisulfite, potassium sulfite, potassium metabisulfite, Glydant ® Plus, Phenonip ® , methylparaben, Germall ® 115, Germaben ® II, Neolone ® , Kathon ® , and Euxyl ® . [0352] Exemplary buffering agents include citrate buffer solutions, acetate buffer solutions, phosphate buffer solutions, ammonium chloride, calcium carbonate, calcium chloride, calcium citrate, calcium glubionate, calcium gluceptate, calcium gluconate, D-gluconic acid, calcium glycerophosphate, calcium lactate, propanoic acid, calcium levulinate, pentanoic acid, dibasic calcium phosphate, phosphoric acid, tribasic calcium phosphate, calcium hydroxide phosphate, potassium acetate, potassium chloride, potassium gluconate, potassium mixtures, dibasic potassium phosphate, monobasic potassium phosphate, potassium phosphate mixtures, sodium acetate, sodium bicarbonate, sodium chloride, sodium citrate, sodium lactate, dibasic sodium phosphate, monobasic sodium phosphate, sodium phosphate mixtures, tromethamine, magnesium hydroxide, aluminum hydroxide, alginic acid, pyrogen-free water, isotonic saline, Ringer’s solution, ethyl alcohol, and mixtures thereof. [0353] Exemplary lubricating agents include magnesium stearate, calcium stearate, stearic acid, silica, talc, malt, glyceryl behanate, hydrogenated vegetable oils, polyethylene glycol, sodium benzoate, sodium acetate, sodium chloride, leucine, magnesium lauryl sulfate, sodium lauryl sulfate, and mixtures thereof. [0354] Exemplary natural oils include almond, apricot kernel, avocado, babassu, bergamot, black current seed, borage, cade, camomile, canola, caraway, carnauba, castor, cinnamon, cocoa butter, coconut, cod liver, coffee, corn, cotton seed, emu, eucalyptus, evening primrose, fish, flaxseed, geraniol, gourd, grape seed, hazel nut, hyssop, isopropyl myristate, jojoba, kukui nut, lavandin, lavender, lemon, litsea cubeba, macademia nut, mallow, mango seed, meadowfoam seed, mink, nutmeg, olive, orange, orange roughy, palm, palm kernel, peach kernel, peanut, poppy seed, pumpkin seed, rapeseed, rice bran, rosemary, safflower, sandalwood, sasquana, savoury, sea buckthorn, sesame, shea butter, silicone, soybean, sunflower, tea tree, thistle, tsubaki, vetiver, walnut, and wheat germ oils. Exemplary synthetic oils include butyl stearate, caprylic triglyceride, capric triglyceride, cyclomethicone, diethyl sebacate, dimethicone 360, isopropyl myristate, mineral oil, octyldodecanol, oleyl alcohol, silicone oil, and mixtures thereof. [0355] In certain embodiments, the compositions further comprise an agent, and are useful for delivering said agent (e.g., to a subject, tissue, biological sample, or cell). In certain embodiments, the compositions are pharmaceutical compositions which are useful for treating a disease in a subject in need thereof. In certain embodiments, the disease is cancer. In certain embodiments, the cancer is colorectal cancer (e.g., colon cancer or rectal cancer). In certain embodiments, the cancer is gastric cancer. In certain embodiments, the cancer is gastrointestinal stromal tumor. In certain embodiments, the cancer is ovarian cancer (e.g., ovarian adenocarcinoma). In certain embodiments, the cancer is lung cancer (e.g., small cell lung cancer). In certain embodiments, the cancer is non-small cell lung cancer. In certain embodiments, the cancer is breast cancer. In certain embodiments, the cancer is pancreatic cancer (e.g., pancreatic carcinoma or pancreatic adenocarcinoma). In certain embodiments, the cancer is prostate cancer (e.g., prostate adenocarcinoma). In certain embodiments, the cancer is testicular cancer. In certain embodiments, the cancer is liver cancer. In certain embodiments, the cancer is endometrial cancer (e.g., uterine cancer). In certain embodiments, the cancer is lymphoma, such as non-Hodgkin’s lymphoma (e.g., B-cell non-Hodgkin’s lymphoma). In certain embodiments, the cancer is B-cell lymphoma (e.g., Burkitt’s B-cell lymphoma, large B-cell lymphoma). In certain embodiments, the cancer is T-cell lymphoma. In certain embodiments, the cancer is Burkitt’s lymphoma (e.g., Burkitt’s B-cell lymphoma). In certain embodiments, the cancer is large cell immunoblastic lymphoma. In certain embodiments, the cancer is leukemia. In certain embodiments, the cancer is acute lymphocytic leukemia (ALL), acute myelogenous leukemia (AML) (also known as acute myeloid leukemia, acute myeloblastic leukemia, acute granulocytic leukemia, acute myelocytic leukemia, and acute nonlymphocytic leukemia), chronic lymphocytic leukemia (CLL), or chronic myelogenous leukemia (CML) (also known as chronic myeloid leukemia). In certain embodiments, the cancer is AML. In some embodiments, the cancer is a subtype of AML selected from undifferentiated acute myeloblastic leukemia (M0), acute myeloblastic leukemia with minimal maturation (M1), acute myeloblastic leukemia with maturation (M2), acute promyelocytic leukemia (APL) (M3), acute myelomonocytic leukemia (M4), acute myelomonocytic leukemia with eosinophilia (M4 eos), acute monocytic leukemia (M5), acute erythroid leukemia (M6), and acute megakaryoblastic leukemia (M7). In certain embodiments, the cancer is acute monocytic leukemia or acute lymphocytic leukemia (e.g., B-cell acute lymphocytic leukemia). In certain embodiments, the cancer is acute lymphoblastic leukemia (e.g., B-cell acute lymphoblastic leukemia or T-cell acute lymphoblastic leukemia). In certain embodiments, the cancer is multiple myeloma (e.g., B-cell myeloma). [0356] A composition, as described herein, can be administered in combination with one or more additional agents. In certain embodiments, the agents are organic molecules. In certain embodiments, the agents are inorganic molecules. In certain embodiments, the agents are targeting agents. In certain embodiments, the agents are isotopically labeled chemical compounds. In certain embodiments, the agents are agents useful in bioprocessing. In certain embodiments, the agents are pharmaceutical agents (e.g., therapeutically and/or prophylactically active agents). Pharmaceutical agents include therapeutically active agents. Pharmaceutical agents also include prophylactically active agents. Pharmaceutical agents include small organic molecules such as drug compounds (e.g., compounds approved for human or veterinary use by the U.S. Food and Drug Administration as provided in the Code of Federal Regulations (CFR)), peptides, proteins, carbohydrates, monosaccharides, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides or proteins, small molecules linked to proteins, glycoproteins, steroids, nucleic acids, DNAs, RNAs, nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides, polynucleotides, lipids, hormones, vitamins, vaccines, immunological agents, and cells.
[0357] In certain embodiments, the compound of the disclosure described herein is provided in an effective amount in the composition. In certain embodiments, the effective amount is a therapeutically effective amount. In certain embodiments, the effective amount is an amount effective for treating cancer in a subject in need thereof. In certain embodiments, the effective amount is an amount effective for inhibiting the signaling pathway required for metastasis in a subject or cell.
[0358] In certain embodiments, the effective amount is an amount effective for inhibiting the activity of NAMPT by at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, or at least about 98%. In certain embodiments, the effective amount is an amount effective for inhibiting the activity of NAMPT by not more than 10%, not more than 20%, not more than 30%, not more than 40%, not more than 50%, not more than 60%, not more than 70%, not more than 80%, not more than 90%, not more than 95%, or not more than 98%. In certain embodiments, the effective amount is an amount effective for inhibiting the activity of NAMPT by a range between a percentage described in this paragraph and another percentage described in this paragraph, inclusive.
[0359] In certain embodiments, the effective amount is an amount effective for increasing the activity of NAMPT by at least 5%, at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.5%, at least 99.9%, at least 99.99%, or at least 99.999% of an initial level, which may, for example, be a baseline level of enzyme activity. In certain embodiments, the effective amount is an amount effective for increasing the activity of NAMPT by not more than 10%, not more than 20%, not more than 30%, not more than 40%, not more than 50%, not more than 60%, not more than 70%, not more than 80%, not more than 90%, not more than 95%, not more than 98%, or not more than 99.9%. In certain embodiments, the effective amount is an amount effective for increasing the activity of NAMPT by a range between a percentage described in this paragraph and another percentage described in this paragraph, inclusive. [0360] In certain embodiments, the effective amount is an amount effective for inhibiting the signaling of NAMPT by at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, or at least about 98%. In certain embodiments, the effective amount is an amount effective for inhibiting the signaling of NAMPT by not more than 10%, not more than 20%, not more than 30%, not more than 40%, not more than 50%, not more than 60%, not more than 70%, not more than 80%, not more than 90%, not more than 95%, or not more than 98%. In certain embodiments, the effective amount is an amount effective for inhibiting the signaling of NAMPT by a range between a percentage described in this paragraph and another percentage described in this paragraph, inclusive. [0361] In certain embodiments, the effective amount is an amount effective for increasing the signaling of NAMPT by at least 5%, at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.5%, at least 99.9%, at least 99.99%, or at least 99.999% of an initial level, which may, for example, be a baseline level of enzyme activity. In certain embodiments, the effective amount is an amount effective for increasing the signaling of NAMPT by not more than 10%, not more than 20%, not more than 30%, not more than 40%, not more than 50%, not more than 60%, not more than 70%, not more than 80%, not more than 90%, not more than 95%, not more than 98%, or not more than 99.9%. In certain embodiments, the effective amount is an amount effective for increasing the signaling of NAMPT by a range between a percentage described in this paragraph and another percentage described in this paragraph, inclusive. [0362] In certain embodiments, the cell is in vitro. In certain embodiments, the cell is ex vivo. In certain embodiments, the cell is in vivo. [0363] Compositions may be formulated into liquid dosage forms for oral and parenteral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the agents, the liquid dosage forms may comprise inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (e.g., cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents. In certain embodiments for parenteral administration, the compositions described herein are mixed with solubilizing agents such as Cremophor ® , alcohols, oils, modified oils, glycols, polysorbates, cyclodextrins, polymers, and mixtures thereof. [0364] Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions can be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation can be a sterile injectable solution, suspension, or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are water, Ringer’s solution, U.S.P., and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or di-glycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables. [0365] The injectable formulations can be sterilized, for example, by filtration through a bacterial- retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use. [0366] In order to prolong the effect of a compound of the disclosure, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This can be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution, which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form may be accomplished by dissolving or suspending the compound in an oil vehicle. [0367] Compositions for rectal or vaginal administration are typically suppositories which can be prepared by mixing the compositions described herein with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol, or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the compound of the disclosure. [0368] Compositions may be formulated into solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the compound of the disclosure is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or (a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, (b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, (c) humectants such as glycerol, (d) disintegrating agents such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, (e) solution retarding agents such as paraffin, (f) absorption accelerators such as quaternary ammonium compounds, (g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, (h) absorbents such as kaolin and bentonite clay, and (I) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets, and pills, the dosage form may include a buffering agent. [0369] Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the art of pharmacology. They may optionally comprise opacifying agents and can be of a composition that they release the compound of the disclosure only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of encapsulating compositions which can be used include polymeric substances and waxes. Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. [0370] The compound of the disclosure can be in a micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings, and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the compound of the disclosure can be admixed with at least one inert diluent such as sucrose, lactose, or starch. Such dosage forms may comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may comprise buffering agents. They may optionally comprise opacifying agents and can be of a composition that they release the compound of the disclosure only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of encapsulating agents which can be used include polymeric substances and waxes. [0371] Dosage forms for topical and/or transdermal administration of a composition described herein may include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants, and/or patches. Generally, the compound of the disclosure is admixed under sterile conditions with a pharmaceutically acceptable carrier or excipient and/or any needed preservatives and/or buffers as can be required. [0372] Suitable devices for use in delivering intradermal compositions described herein include short needle devices. Intradermal compositions can be administered by devices which limit the effective penetration length of a needle into the skin. Alternatively, or additionally, conventional syringes can be used in the classical mantoux method of intradermal administration. Jet injection devices which deliver liquid formulations to the dermis via a liquid jet injector and/or via a needle which pierces the stratum corneum and produces a jet which reaches the dermis are suitable. Ballistic powder/particle delivery devices which use compressed gas to accelerate the polymer in powder form through the outer layers of the skin to the dermis are suitable. [0373] Formulations suitable for topical administration include liquid and/or semi-liquid preparations such as liniments, lotions, oil-in-water and/or water-in-oil emulsions such as creams, ointments, and/or pastes, and/or solutions and/or suspensions. Topically administrable formulations may, for example, comprise from about 1% to about 100% (w/w) compound of the disclosure, although the concentration of the compound of the disclosure can be as high as the solubility limit of the compound of the disclosure in the solvent. Formulations for topical administration may further comprise one or more of the additional ingredients described herein.
[0374] A composition described herein can be prepared, packaged, and/or sold in a formulation suitable for pulmonary administration via the buccal cavity. Such a formulation may comprise dry particles which comprise the compound of the disclosure. Such compositions are conveniently in the form of dry powders for administration using a device comprising a dry powder reservoir to which a stream of propellant can be directed to disperse the powder and/or using a self-propelling solvent/powder dispensing container such as a device comprising the agent dissolved and/or suspended in a low-boiling propellant in a sealed container. Dry powder compositions may include a solid fine powder diluent such as sugar and are conveniently provided in a unit dose form.
[0375] Low boiling propellants generally include liquid propellants having a boiling point of below 65 °F at atmospheric pressure. Generally, the propellant may constitute 50 to 99.9% (w/w) of the composition, and the compound of the disclosure may constitute 0.1 to 100% (w/w) of the composition. The propellant may further comprise additional ingredients such as a liquid non-ionic and/or solid anionic surfactant and/or a solid diluent.
[0376] Compositions described herein formulated for pulmonary delivery may provide the compound of the disclosure in the form of droplets of a solution and/or suspension. Such formulations can be prepared, packaged, and/or sold as aqueous and/or dilute alcoholic solutions and/or suspensions, optionally sterile, comprising the compound of the disclosure, and may conveniently be administered using any nebulization and/or atomization device. Such formulations may further comprise one or more additional ingredients including a flavoring agent such as saccharin sodium, a volatile oil, a buffering agent, a surface active agent, and/or a preservative such as methylhydroxybenzoate.
[0377] Formulations described herein as being useful for pulmonary delivery are useful for intranasal delivery of a pharmaceutical composition described herein. Another formulation suitable for intranasal administration is a coarse powder comprising the compound of the disclosure. Such a formulation is administered by rapid inhalation through the nasal passage from a container of the powder held close to the nares.
[0378] Formulations for nasal administration may, for example, comprise from about as little as 0.1% (w/w) to as much as 100% (w/w) of the compound of the disclosure, and may comprise one or more of the additional ingredients described herein. A pharmaceutical composition described herein can be prepared, packaged, and/or sold in a formulation for buccal administration. Such formulations may, for example, be in the form of tablets and/or lozenges made using conventional methods, and may contain, for example, 0.1 to 20% (w/w) agent, the balance comprising an orally dissolvable and/or degradable composition and, optionally, one or more of the additional ingredients described herein. Alternately, formulations for buccal administration may comprise a powder and/or an aerosolized and/or atomized solution and/or suspension comprising the compound of the disclosure. [0379] A composition described herein can be prepared, packaged, and/or sold in a formulation for ophthalmic administration. Such formulations may, for example, be in the form of eye drops including, for example, a 0.1-100% (w/w) solution and/or suspension of the compound of the disclosure in an aqueous or oily liquid carrier or excipient. Such drops may further comprise buffering agents, salts, and/or one or more other of the additional ingredients described herein. Other ophthalmically-administrable formulations which are useful include those which comprise the compound of the disclosure in microcrystalline form and/or in a liposomal preparation. Ear drops and/or eye drops are also contemplated as being within the scope of this disclosure.
[0380] Although the descriptions of compositions provided herein are principally directed to compositions which are suitable for administration to humans, it will be understood by the skilled artisan that such compositions are generally suitable for administration to animals of all sorts. Modification of compositions suitable for administration to humans in order to render the compositions suitable for administration to various animals is well understood, and the ordinarily skilled veterinary pharmacologist can design and/or perform such modification with ordinary experimentation.
[0381] Compositions provided herein are typically formulated in dosage unit form for ease of administration and uniformity of dosage. It will be understood, however, that the total daily usage of the compositions described herein will be decided by a physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular subject or organism will depend upon a variety of factors including the cancer being treated and the severity of the cancer; the activity of the specific compound of the disclosure employed; the specific composition employed; the age, body weight, general health, sex, and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific compound of the disclosure employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound of the disclosure employed; and like factors well known in the medical arts.
[0382] The compositions provided herein can be administered by any route, including enteral (e.g. , oral), parenteral, intravenous, intramuscular, intra-arterial, intramedullary, intrathecal, subcutaneous, intraventricular, transdermal, intradermal, rectal, intravaginal, intraperitoneal, topical (as by powders, ointments, creams, and/or drops), mucosal, nasal, buccal, sublingual; by intratracheal instillation, bronchial instillation, and/or inhalation; and/or as an oral spray, nasal spray, and/or aerosol. Specifically, contemplated routes are oral administration, intravenous administration (e.g., systemic intravenous injection), regional administration via blood and/or lymph supply, and/or direct administration to an affected site. In general, the most appropriate route of administration will depend upon a variety of factors including the nature of the compound of the disclosure (e.g., its stability in the environment of the gastrointestinal tract), and/or the condition of the subject (e.g., whether the subject is able to tolerate oral administration). In certain embodiments, the composition described herein is suitable for topical administration to the eye of a subject. [0383] In some embodiments, administration of any of the compositions described herein occurs at least one hour prior to treatment with another cancer therapy. [0384] The compositions can be administered in combination with additional agents that improve their activity (e.g., potency and/or efficacy) in treating a disease or disorder (e.g., cancer) in a subject in need thereof and/or in inhibiting the signaling pathway in a subject or cell), improve bioavailability, improve safety, reduce drug resistance, reduce and/or modify metabolism, inhibit excretion, and/or modify distribution in a subject or cell. It will also be appreciated that the therapy employed may achieve a desired effect for the same disorder, and/or it may achieve different effects. In certain embodiments, a composition described herein, including a compound of the disclosure described herein, and an agent show a synergistic effect that is absent in a composition including one of the compounds of the disclosure or the agent, but not both. [0385] The composition can be administered concurrently with, prior to, or subsequent to one or more additional agents, which are different from the composition and may be useful as, e.g., combination therapies. Each additional pharmaceutical agent may be administered at a dose and/or on a time schedule determined for that pharmaceutical agent. The additional pharmaceutical agents may also be administered together with each other and/or with the compound of the disclosure or composition described herein in a single dose or administered separately in different doses. The particular combination to employ in a regimen will take into account compatibility of the compound of the disclosure described herein with the additional pharmaceutical agent(s) and/or the desired therapeutic and/or prophylactic effect to be achieved. In general, it is expected that the additional pharmaceutical agent(s) in combination be utilized at levels that do not exceed the levels at which they are utilized individually. In some embodiments, the levels utilized in combination will be lower than those utilized individually. [0386] The additional pharmaceutical agents include anti-proliferative agents, anti-cancer agents, cytotoxic agents, anti-angiogenesis agents, anti-inflammatory agents, immunosuppressants, anti- bacterial agents, anti-viral agents, cardiovascular agents, cholesterol-lowering agents, anti-diabetic agents, anti-allergic agents, contraceptive agents, and pain-relieving agents. In certain embodiments, the additional pharmaceutical agent is an anti-proliferative agent. In certain embodiments, the additional pharmaceutical agent is an anti-cancer agent. In certain embodiments, the additional pharmaceutical agent is a chemotherapeutic agent. In certain embodiments, the additional pharmaceutical agent is a differentiation agent (e.g., retinoids, all-trans retinoic acid (ATRA), vitamin D, peroxisome proliferator activated receptor gamma (PPAR gamma) inhibitors). In certain embodiments, the additional pharmaceutical agent is an anti-viral agent. In certain embodiments, the additional pharmaceutical agent is a binder or inhibitor of a protein kinase. In certain embodiments, the additional pharmaceutical agent is selected from the group consisting of epigenetic or transcriptional modulators (e.g., DNA methyltransferase inhibitors, histone deacetylase inhibitors (HDAC inhibitors), lysine methyltransferase inhibitors), antimitotic drugs (e.g., taxanes and vinca alkaloids), hormone receptor modulators (e.g., estrogen receptor modulators and androgen receptor modulators), cell signaling pathway inhibitors (e.g., tyrosine protein kinase inhibitors), modulators of protein stability (e.g., proteasome inhibitors), Hsp90 inhibitors, glucocorticoids, all-trans retinoic acids, and other agents that promote differentiation. In certain embodiments, a compound disclosed herein or pharmaceutical composition can be administered in combination with an anti-cancer therapy including surgery, radiation therapy, transplantation (e.g., stem cell transplantation, bone marrow transplantation), immunotherapy, and chemotherapy. In some embodiments, the subject is administered concurrently with, prior to, or subsequent to one or more additional agents, such as one or more additional cancer therapies. In some embodiments, the one or more additional cancer therapy includes an immunotherapy. In general, immunotherapy, also called biologic therapy, is a type of cancer treatment that boosts a subject’s natural defenses to treat cancer. In certain embodiments, the immunotherapy utilizes compounds biologically produced by the subject. In certain embodiments, the immunotherapy utilizes compounds not biologically produced by the subject. In certain embodiments, the immunotherapy utilizes cells from the subject. In certain embodiments, the immunotherapy utilizes cells not from the subject. In certain embodiments, the immunotherapy utilizes compounds biologically produced by an organism that is not the subject. In certain embodiments, the immunotherapy utilizes cells biologically produced by an organism that is not the subject. In certain embodiments, the immunotherapy includes at least one chemical modification to compounds or cells from the subject. In certain embodiments, the immunotherapy includes at least one chemical modification to compounds or cells not from the subject. [0387] In some embodiments, the additional pharmaceutical agent is NAM. [0388] In some embodiments, the additional pharmaceutical agent is NMN. [0389] In some embodiments, the additional pharmaceutical agent is selected from the group consisting of nicotinic acid (e.g., niacin), ibrutinib, idelalisib, lenalidomide, a BCL-2 inhibitor, venetoclax, FLT3 inhibitor, IDH 1/2 inhibitor, glasdegib, azacytidine, cyclophosphamide, decitabine, enasidenib, erastin, gilterinib, idasanutlin, ivosidenib, ixazomib, midostaurin, navitoclax, onvasertib, cyclosporin A, PARP inhibitor, pacritinib, phorbol 12-myristate 13-acetate (PMA), ruxolitinib, S055746, selinexor (KPT-330), trametinib, tretinoin, etoposide, P7C3, napabucasin, olaparib, AraC, daunorubicin, 1-methyl-3-nitro-1-nitrosoguanidinium (MNNG), melphalan, verapamil, etoposide, cisplatin, anti-PD1, tumor necrosis factor−related apoptosis-inducing ligand (TRAIL), EX527, sirtinol, cambinol, vorinostat, valproic acid, butyrate, JPH203, L-asparaginase, bortezomib, rituximab, PGP-4008, β-lapachone, β-methylene adenosine 5′-diphosphate (APCP), gemcitabine, Lu- DOTATATE, fluorouracil (5-FU), pemetrexed, onvansertib, cedazuridine, galinpepimut-S, cedazuridine/decitabine, cytarabine/daunorubicin, uproleselan, gedatolisib, devimistat, glasdegib, idasanutlin, ganetespib, tipifarnib, midostaurin, pevonedistat, ivosidenib, crenolanib, quizartinib, pracinostat, guadecitabine, DFP 10917, vosaroxin, rexlemestrocel-L-mesoblast, treosulfan, sapacitabine, enasidenib, volasertib, and temozolomide. [0390] In some embodiments, the BCL-2 inhibitor is veneteoclax, navitoclax, oblimersen, APG 2575, BCL201, BGB-11417, LP-108, or S65487. [0391] In some embodiments, the additional pharmaceutical agent is a Toll like Receptor 4 (TLR4) inhibitor. In some embodiments, the Toll like Receptor 4 inhibitor is TAK-242, E5564, OM-174, GSK1795091, GLA-SE, NI-0101, AV-411, AS04, amitriptyline, cyclobenzaprine, ketotifen, imipramine, mianserin, ibudilast, pinocembrin, resatorvid, naloxone, naltrexone, LPS-RS, propentofylline, tapentadol, palmitoylethanolamide, [0392] In some embodiments, the additional pharmaceutical agent is a sirtuin inhibitor. In some embodiments, the sirtuin inhibitor is nicotinamide, a nicotinamide derivative, benzamide, 3′- phenethyloxy-2-anilino benzamide analogues, AK7, 1,4-dihydropyridine, cambinol, EX-527, AGK2, 3′-(3-fluoro-phenethyloxy)-2-anilinobenzamide, SirReal2, UBCS0137, ELT-11c, or thioacyllysine- containing compounds. [0393] In some embodiments, the additional pharmaceutical agent is selected ibrutinib or idelalisib. [0394] In some embodiments, the additional pharmaceutical agent is lenalidomide. [0395] In some embodiments, the additional pharmaceutical agent is selected from the group consisting of venetoclax, FLT3 inhibitor, IDH 1/2 inhibitor, glasdegib, azacytidine, cyclophosphamide, decitabine, enasidenib, erastin, gilterinib, idasanutlin, ivosidenib, ixazomib, midostaurin, navitoclax, onvasertib, pacritinib, PMA, ruxolitinib, S055746, selinexor (KPT-330), trametinib, tretinoin, etoposide, and napabucasin. [0396] In some embodiments, the additional pharmaceutical agent is P7C3. [0397] In some embodiments, the FLT3 inhibitor is selected from the group consisting of sorafenib, sunitinib, lestaurtinib, tandutinib, ponatinib, midostaurin, gilteritinib, quizartinib, crenolanib, cabozantinib, ibrutinib, and KW-2449. In some embodiments, the FLT3 inhibitor is sorafenib, sunitinib, ponatinib, cabozantinib, ibrutinib, midostaurin, or gilteritinib. In some embodiments, the FLT3 inhibitor is midostaurin or gilteritinib. [0398] In some embodiments, the IDH 1/2 inhibitor is selected from the group consisting of ivosidenib and enasidenib. [0399] In some embodiments, the PARP inhibitor is selected from the group consisting of olaparib, rucaparib, niraparib, talazoparib, veliparib, pamiparib, CEP 9722, CEP-8983, E7016, iniparib, and 3- aminobenzamide. In some embodiments, the PARP inhibitor is selected from the group consisting of olaparib, rucaparib, niraparib, and talazoparib. In some embodiments, the PARP inhibitor is selected from the group consisting of veliparib, pamiparib, CEP 9722, CEP-8983, E7016, iniparib, and 3- aminobenzamide. [0400] In certain embodiments, the additional pharmaceutical agent is an HDAC inhibitor. [0401] In certain embodiments, the additional pharmaceutical agent is nicotinic acid (e.g., niacin). [0402] In some embodiments, the additional pharmaceutical agent is afatinib, afatinib dimaleate, alectinib, atezolizumab, bevacizumab, brigatinib, capmatinib, capmatinib hydrochloride, carboplatin, carboplatin-taxol, ceritinib, crizotinib, dabrafenib, dabrafenib mesylate, dacomitinib, docetaxel, doxorubicin, doxorubicin hydrochloride, durvalumab, entrectinib, erlotinib, erlotinib hydrochloride, everolimus, etoposide phosphate, etoposide, gefitinib, gemcitabine, gemcitabine hydrochloride, gemcitabine-cisplatin, ipilimumab, lorlatinib, lurbinectedin, methotrexate, methotrexate sodium, necitumumab, nivolumab, osimertinib mesylate, osimertinib, paclitaxel, paclitaxel albumin-stabilized nanoparticle formulation, pembrolizumab, pralsetinib, pemetrexed, pemetrexed disodium, ramucirumab, selpercatinib, topotecan, topotecan hydrochloride, trametinib, vinorelbine, or vinorelbine tartrate. [0403] In some embodiments, the additional pharmaceutical agent is selected from the group consisting of afatinib, afatinib dimaleate, albumin-bound paclitaxel, alectinib, atezolizumab, atezolizumab and durvalumab, bevacizumab, brigatinib, capmatinib, capmatinib hydrochloride, carboplatin, carboplatin and etoposide, carboplatin and irinotecan, carboplatin and paclitaxel, cemiplimab, cemiplimab-rwlc, ceritinib, cisplatin, cisplatin and etoposide, cisplatin and irinotecan, crizotinib, cyclophosphamide, dabrafenib, dabrafenib mesylate, dacomitinib, docetaxel, doxorubicin hydrochloride, durvalumab, entrectinib, erlotinib, erlotinib and ramucirumab, erlotinib hydrochloride, etoposide, etoposide phosphate, everolimus, gefitinib, gemcitabine, gemcitabine and cisplatin, gemcitabine hydrochloride, icotinib, ifosfamide, ipilimumab, irinotecan, larotrectinib, lorlatinib, lurbinectedin, methotrexate sodium, nab-paclitaxel, necitumumab, nivolumab, osimertinib, osimertinib mesylate, paclitaxel, paclitaxel albumin-stabilized nanoparticle formulation, pembrolizumab, pemetrexed, pemetrexed disodium, pralsetinib, ramucirumab, rinotecan hydrochloride, selpercatinib, tepotinib, tepotinib hydrochloride, topotecan, topotecan hydrochloride, trametinib, trametinib dimethyl sulfoxide, vincristine sulfate, vinorelbine, and vinorelbine tartrate. [0404] In some embodiments, the additional pharmaceutical agent is selected from the group consisting of topoisomerase II inhibitors (e.g., etoposide, doxorubicin), topoisomerase I inhibitors (e.g., irinotecan, CPT-11, camptostar, topotecan), tubulin interacting agents (e.g., paclitaxel, docetaxel, epothilones), thymidilate synthase inhibitors (e.g., 5-fluorouracil or 5-FU), alkylating agents (e.g., temozolomide, cyclophosphamide), farnesyl protein transferase inhibitors (e.g., Lonafarnib, L778,123, BMS 214662), signal transduction inhibitors (e.g., gefitinib), antibodies to EGFR (e.g., cetuximab), cytarabine, aromatase inhibitors (e.g., exemestance, anastrozole, letrozole). [0405] In some embodiments, the additional pharmaceutical agent is cytarabine. [0406] In some embodiments, the additional pharmaceutical agent is an aromatase inhibitor. In some embodiments, the aromatase inhibitor is aminoglutethimide, testolactone, anastrozole, letrozole, exemestane, vorozole, formestane, fadrozole, 1,4,6-androstatrien-3,17-dione, or 4-androstene-3,6,17- trione. [0407] In certain embodiments, the additional pharmaceutical agent is an anti-cancer or anti- neoplastic agent. In some embodiments, the additional pharmaceutical agent is cytarabine, doxorubicin, cyclophosphamide, FX-11, uracil mustard, chlormethine, hexamethylmelamine, zevalin, trisenox, xeloda, aminoglutethimide, 6-thioguanine, pentostatin, mithramycin, ifosfamide, pipobroman, triethylenemelamine, methyltestosterone, amsacrine, mitotane, levamisole, triamcinolone, testosterone, fluoxymesterone, dromostanolone propionate, triethylenethiophosphoramine, streptozocin, 6-mercaptopurine, deoxycoformycin, mitomycin-C, 17a- ethinylestradiol, diethylstilbestrol, testolactone, megestrolacetate, methylprednisolone, chlorotrianisene, hydroxyprogesterone, medroxyprogesteroneacetate, toremifene, navelbene, anastrazole, letrazole, reloxafme, droloxafme, porfimer, thiotepa, altretamine, lerozole, fulvestrant, exemestane, 5-HT3 receptor inhibitor (e.g., dolansetron, granisetron, ondansetron) or dexamethasone. [0408] In some embodiments, the additional pharmaceutical agent is cytarabine, doxorubicin, cyclophosphamide, FX-11, uracil mustard, chlormethine, hexamethylmelamine, zevalin, trisenox, xeloda, aminoglutethimide, 6-thioguanine, pentostatin, mithramycin, ifosfamide, pipobroman, triethylenemelamine, methyltestosterone, amsacrine, mitotane, levamisole, triamcinolone, testosterone, fluoxymesterone, dromostanolone propionate, triethylenethiophosphoramine, streptozocin, 6-mercaptopurine, deoxycoformycin, mitomycin-C, 17a-ethinylestradiol, diethylstilbestrol, testolactone, megestrolacetate, methylprednisolone, chlorotrianisene, hydroxyprogesterone, medroxyprogesteroneacetate, toremifene, navelbene, anastrazole, letrazole, reloxafme, droloxafme, porfimer, thiotepa, altretamine, lerozole, fulvestrant, exemestane, or dexamethasone, with a 5-HT3 receptor inhibitor (e.g., dolansetron, granisetron, ondansetron). [0409] In some embodiments, the additional pharmaceutical agent is cytarabine, doxorubicin, cyclophosphamide, FX-11, uracil mustard, chlormethine, hexamethylmelamine, zevalin, trisenox, xeloda, aminoglutethimide, 6-thioguanine, pentostatin, mithramycin, ifosfamide, pipobroman, triethylenemelamine, methyltestosterone, amsacrine, mitotane, levamisole, triamcinolone, testosterone, fluoxymesterone, dromostanolone propionate, triethylenethiophosphoramine, streptozocin, 6-mercaptopurine, deoxycoformycin, mitomycin-C, 17a-ethinylestradiol, diethylstilbestrol, testolactone, megestrolacetate, methylprednisolone, chlorotrianisene, hydroxyprogesterone, medroxyprogesteroneacetate, toremifene, navelbene, anastrazole, letrazole, reloxafme, droloxafme, porfimer, thiotepa, altretamine, lerozole, fulvestrant, exemestane, or a 5-HT3 receptor inhibitor (e.g., dolansetron, granisetron, ondansetron), with dexamethasone. [0410] In some embodiments, the additional pharmaceutical agent is cytarabine. In some embodiments, the additional pharmaceutical agent is doxorubicin. In some embodiments, the additional pharmaceutical agent is cyclophosphamide. In some embodiments, the additional pharmaceutical agent is FX-11. In certain embodiments, the additional pharmaceutical agent is uracil mustard. In some embodiments, the additional pharmaceutical agent is chlormethine. In some embodiments, the additional pharmaceutical agent is hexamethylmelamine. In some embodiments, the additional pharmaceutical agent is zevalin. In some embodiments, the additional pharmaceutical agent is trisenox. In some embodiments, the additional pharmaceutical agent is xeloda. In some embodiments, the additional pharmaceutical agent is aminoglutethimide. In some embodiments, the additional pharmaceutical agent is 6-thioguanine. In some embodiments, the additional pharmaceutical agent is pentostatin. In some embodiments, the additional pharmaceutical agent is mithramycin. In some embodiments, the additional pharmaceutical agent is ifosfamide. In some embodiments, the additional pharmaceutical agent is pipobroman. In some embodiments, the additional pharmaceutical agent is triethylenemelamine. In some embodiments, the additional pharmaceutical agent is methyltestosterone. In some embodiments, the additional pharmaceutical agent is amsacrine. In some embodiments, the additional pharmaceutical agent is mitotane. In some embodiments, the additional pharmaceutical agent is levamisole. In some embodiments, the additional pharmaceutical agent is triamcinolone. In some embodiments, the additional pharmaceutical agent is testosterone. In some embodiments, the additional pharmaceutical agent is fluoxymesterone. In some embodiments, the additional pharmaceutical agent is dromostanolone propionate. In some embodiments, the additional pharmaceutical agent is triethylenethiophosphoramine. In some embodiments, the additional pharmaceutical agent is streptozocin. In some embodiments, the additional pharmaceutical agent is 6-mercaptopurine. In some embodiments, the additional pharmaceutical agent is deoxycoformycin. In some embodiments, the additional pharmaceutical agent is mitomycin-C. In some embodiments, the additional pharmaceutical agent is 17a-ethinylestradiol. In some embodiments, the additional pharmaceutical agent is diethylstilbestrol. In some embodiments, the additional pharmaceutical agent is testolactone. In some embodiments, the additional pharmaceutical agent is megestrolacetate. In some embodiments, the additional pharmaceutical agent is methylprednisolone. In some embodiments, the additional pharmaceutical agent is chlorotrianisene. In some embodiments, the additional pharmaceutical agent is hydroxyprogesterone. In some embodiments, the additional pharmaceutical agent is medroxyprogesteroneacetate. In some embodiments, the additional pharmaceutical agent is toremifene. In some embodiments, the additional pharmaceutical agent is navelbene. In some embodiments, the additional pharmaceutical agent is anastrazole. In some embodiments, the additional pharmaceutical agent is letrazole. In some embodiments, the additional pharmaceutical agent is reloxafme. In some embodiments, the additional pharmaceutical agent is droloxafme. In some embodiments, the additional pharmaceutical agent is porfimer. In some embodiments, the additional pharmaceutical agent is thiotepa. In some embodiments, the additional pharmaceutical agent is altretamine. In some embodiments, the additional pharmaceutical agent is lerozole. In some embodiments, the additional pharmaceutical agent is fulvestrant. In some embodiments, the additional pharmaceutical agent is exemestane. In some embodiments, the additional pharmaceutical agent is a 5-HT3 receptor inhibitor. In some embodiments, the additional pharmaceutical agent is dolansetron. In some embodiments, the additional pharmaceutical agent is granisetron. In some embodiments, the additional pharmaceutical agent is ondansetron. In some embodiments, the additional pharmaceutical agent is dexamethasone. [0411] In some embodiments, the additional pharmaceutical agent is for treating lymphoma. In some embodiments, the additional pharmaceutical agent is acalabrutinib, axicabtagene ciloleucel, belinostat, bendamustine, bendamustine hydrochloride, bleomycin, bleomycin sulfate, bortezomib, brentuximab vedotin, brexucabtagene autoleucel, capecitabine, carboplatin, carmustine, chlorambucil, cisplatin, cladribine, copanlisib hydrochloride, cortisone, cortisone acetate, c izotinib, cyclophosphamide, cytarabine, dacarbazine, denileukin diftitox, dexamethasone, dexamethasone acetate, doxorubicin, doxorubicin hydrochloride, duvelisib, etoposide, fludarabine, gemcitabine, ibritumomab tiuxetan, ibrutinib, idelalisib, ifosfamide, lenalidomide, lisocabtagene maraleucel, mechlorethamine, methotrexate, methotrexate sodium, mitoxantone, mogamulizumab-kpke, nelarabine, nivolumab, obinutuzumab, ofatumumab, oxaliplatin, panobinostat, pembrolizumab, pentostatin, plerixafor, polatuzumab vedotin, polatuzumab vedotin-piiq, pralatrexate, prednisone, procarbazine, recombinant interferon alfa-2b, rituximab, romidepsin, selinexor, tafasitamab-cxix, tazemetostat hydrobromide, temsirolimus, thalidomide, thiotepa, tisagenlecleucel, umbralisib tosylate, venetoclax, vinblastine, vinblastine sulfate, vincristine, vincristine sulfate, vorinostat, or zanubrutinib.
[0412] In some embodiments, the additional pharmaceutical agent is acalabrutinib. In some embodiments, the additional pharmaceutical agent is axicabtagene ciloleucel. In some embodiments, the additional pharmaceutical agent is belinostat. In some embodiments, the additional pharmaceutical agent is bendamustine. In some embodiments, the additional pharmaceutical agent is bendamustine hydrochloride. In some embodiments, the additional pharmaceutical agent is bleomycin. In some embodiments, the additional pharmaceutical agent is bleomycin sulfate. In some embodiments, the additional pharmaceutical agent is bortezomib. In some embodiments, the additional pharmaceutical agent is brentuximab vedotin. In some embodiments, the additional pharmaceutical agent is brexucabtagene autoleucel. In some embodiments, the additional pharmaceutical agent is capecitabine. In some embodiments, the additional pharmaceutical agent is carboplatin. In some embodiments, the additional pharmaceutical agent is carmustine. In some embodiments, the additional pharmaceutical agent is chlorambucil. In some embodiments, the additional pharmaceutical agent is cisplatin. In some embodiments, the additional pharmaceutical agent is cladribine. In some embodiments, the additional pharmaceutical agent is copanlisib hydrochloride. In some embodiments, the additional pharmaceutical agent is cortisone. In some embodiments, the additional pharmaceutical agent is cortisone acetate. In some embodiments, the additional pharmaceutical agent is c izotinib. In some embodiments, the additional pharmaceutical agent is cyclophosphamide. In some embodiments, the additional pharmaceutical agent is cytarabine. In some embodiments, the additional pharmaceutical agent is dacarbazine. In some embodiments, the additional pharmaceutical agent is denileukin diftitox. In some embodiments, the additional pharmaceutical agent is dexamethasone. In some embodiments, the additional pharmaceutical agent is dexamethasone acetate. In some embodiments, the additional pharmaceutical agent is doxorubicin. In some embodiments, the additional pharmaceutical agent is doxorubicin hydrochloride. In some embodiments, the additional pharmaceutical agent is duvelisib. In some embodiments, the additional pharmaceutical agent is etoposide. In some embodiments, the additional pharmaceutical agent is fludarabine. In some embodiments, the additional pharmaceutical agent is gemcitabine. In some embodiments, the additional pharmaceutical agent is ibritumomab tiuxetan. In some embodiments, the additional pharmaceutical agent is ibrutinib. In some embodiments, the additional pharmaceutical agent is idelalisib. In some embodiments, the additional pharmaceutical agent is ifosfamide. In some embodiments, the additional pharmaceutical agent is lenalidomide. In some embodiments, the additional pharmaceutical agent is lisocabtagene maraleucel. In some embodiments, the additional pharmaceutical agent is mechlorethamine. In some embodiments, the additional pharmaceutical agent is methotrexate. In some embodiments, the additional pharmaceutical agent is methotrexate sodium. In some embodiments, the additional pharmaceutical agent is mitoxantone. In some embodiments, the additional pharmaceutical agent is mogamulizumab-kpkc. In some embodiments, the additional pharmaceutical agent is nelarabine. In some embodiments, the additional pharmaceutical agent is nivolumab. In some embodiments, the additional pharmaceutical agent is obinutuzumab. In some embodiments, the additional pharmaceutical agent is ofatumumab. In some embodiments, the additional pharmaceutical agent is oxaliplatin. In some embodiments, the additional pharmaceutical agent is panobinostat. In some embodiments, the additional pharmaceutical agent is pembrolizumab. In some embodiments, the additional pharmaceutical agent is pentostatin. In some embodiments, the additional pharmaceutical agent is plerixafor. In some embodiments, the additional pharmaceutical agent is polatuzumab vedotin. In some embodiments, the additional pharmaceutical agent is polatuzumab vedotin-piiq. In some embodiments, the additional pharmaceutical agent is pralatrexate. In some embodiments, the additional pharmaceutical agent is prednisone. In some embodiments, the additional pharmaceutical agent is procarbazine. In some embodiments, the additional pharmaceutical agent is recombinant interferon alfa-2b. In some embodiments, the additional pharmaceutical agent is rituximab. In some embodiments, the additional pharmaceutical agent is romidepsin. In some embodiments, the additional pharmaceutical agent is selinexor. In some embodiments, the additional pharmaceutical agent is tafasitamab-cxix. In some embodiments, the additional pharmaceutical agent is tazemetostat hydrobromide. In some embodiments, the additional pharmaceutical agent is temsirolimus. In some embodiments, the additional pharmaceutical agent is thalidomide. In some embodiments, the additional pharmaceutical agent is thiotepa. In some embodiments, the additional pharmaceutical agent is tisagenlecleucel. In some embodiments, the additional pharmaceutical agent is umbralisib tosylate. In some embodiments, the additional pharmaceutical agent is venetoclax. In some embodiments, the additional pharmaceutical agent is vinblastine. In some embodiments, the additional pharmaceutical agent is vinblastine sulfate. In some embodiments, the additional pharmaceutical agent is vincristine. In some embodiments, the additional pharmaceutical agent is vincristine sulfate. In some embodiments, the additional pharmaceutical agent is vorinostat. In some embodiments, the additional pharmaceutical agent is zanubrutinib.
[0413] In some embodiments, the additional pharmaceutical agent is for treating small cell lung cancer. In some embodiments, the additional pharmaceutical agent is everolimus, atezolizumab, doxorubicin, doxorubicin hydrochloride, durvalumab, etoposide phosphate, etoposide, topotecan, topotecan hydrochloride, pembrolizumab, lurbinectedin, methotrexate, methotrexate sodium, or nivolumab. In some embodiments, the additional pharmaceutical agent is selected from the group consisting of afatinib, afatinib dimaleate, albumin-bound paclitaxel, alectinib, atezolizumab, atezolizumab and durvalumab, bevacizumab, brigatinib, capmatinib, capmatinib hydrochloride, carboplatin, carboplatin and etoposide, carboplatin and irinotecan, carboplatin and paclitaxel, cemiplimab, cemiplimab-rwlc, ceritinib, cisplatin, cisplatin and etoposide, cisplatin and irinotecan, crizotinib, cyclophosphamide, dabrafenib, dabrafenib mesylate, dacomitinib, docetaxel, doxorubicin hydrochloride, durvalumab, entrectinib, erlotinib, erlotinib and ramucirumab, erlotinib hydrochloride, etoposide, etoposide phosphate, everolimus, gefitinib, gemcitabine, gemcitabine and cisplatin, gemcitabine hydrochloride, icotinib, ifosfamide, ipilimumab, irinotecan, larotrectinib, lorlatinib, lurbinectedin, methotrexate sodium, nab-paclitaxel, necitumumab, nivolumab, osimertinib, osimertinib mesylate, paclitaxel, paclitaxel albumin-stabilized nanoparticle formulation, pembrolizumab, pemetrexed, pemetrexed disodium, pralsetinib, ramucirumab, rinotecan hydrochloride, selpercatinib, tepotinib, tepotinib hydrochloride, topotecan, topotecan hydrochloride, trametinib, trametinib dimethyl sulfoxide, vincristine sulfate, vinorelbine, or vinorelbine tartrate [0414] In some embodiments, the additional pharmaceutical agent is afatinib. In some embodiments, the additional pharmaceutical agent is afatinib dimaleate. In some embodiments, the additional pharmaceutical agent is albumin-hound paclitaxel. In some embodiments, the additional pharmaceutical agent is alectinib. In some embodiments, the additional pharmaceutical agent is atezolizumab. In some embodiments, the additional pharmaceutical agent is atezolizumab and durvalumab. In some embodiments, the additional pharmaceutical agent is bevacizumab. In some embodiments, the additional pharmaceutical agent is brigatinib. In some embodiments, the additional pharmaceutical agent is capmatinib. In some embodiments, the additional pharmaceutical agent is capmatinib hydrochloride. In some embodiments, the additional pharmaceutical agent is carboplatin. In some embodiments, the additional pharmaceutical agent is carboplatin and etoposide. In some embodiments, the additional pharmaceutical agent is carboplatin and irinotecan. In some embodiments, the additional pharmaceutical agent is carboplatin and paclitaxel. In some embodiments, the additional pharmaceutical agent is cemiplimab. In some embodiments, the additional pharmaceutical agent is cemiplimab-rwlc. In some embodiments, the additional pharmaceutical agent is ceritinib. In some embodiments, the additional pharmaceutical agent is cisplatin. In some embodiments, the additional pharmaceutical agent is cisplatin and etoposide. In some embodiments, the additional pharmaceutical agent is cisplatin and irinotecan. In some embodiments, the additional pharmaceutical agent is crizotinib. In some embodiments, the additional pharmaceutical agent is cyclophosphamide. In some embodiments, the additional pharmaceutical agent is dabrafenib. In some embodiments, the additional pharmaceutical agent is dabrafenib mesylate. In some embodiments, the additional pharmaceutical agent is dacomitinib. In some embodiments, the additional pharmaceutical agent is docetaxel. In some embodiments, the additional pharmaceutical agent is doxorubicin hydrochloride. In some embodiments, the additional pharmaceutical agent is durvalumab. In some embodiments, the additional pharmaceutical agent is entrectinib. In some embodiments, the additional pharmaceutical agent is erlotinib. In some embodiments, the additional pharmaceutical agent is erlotinib and ramucirumab. In some embodiments, the additional pharmaceutical agent is erlotinib hydrochloride. In some embodiments, the additional pharmaceutical agent is etoposide. In some embodiments, the additional pharmaceutical agent is etoposide phosphate. In some embodiments, the additional pharmaceutical agent is everolimus. In some embodiments, the additional pharmaceutical agent is gefitinib. In some embodiments, the additional pharmaceutical agent is gemcitabine. In some embodiments, the additional pharmaceutical agent is gemcitabine and cisplatin. In some embodiments, the additional pharmaceutical agent is gemcitabine hydrochloride. In some embodiments, the additional pharmaceutical agent is icotinib. In some embodiments, the additional pharmaceutical agent is ifosfamide. In some embodiments, the additional pharmaceutical agent is ipilimumab. In some embodiments, the additional pharmaceutical agent is irinotecan. In some embodiments, the additional pharmaceutical agent is larotrectinib. In some embodiments, the additional pharmaceutical agent is lorlatinib. In some embodiments, the additional pharmaceutical agent is lurbinectedin. In some embodiments, the additional pharmaceutical agent is methotrexate sodium. In some embodiments, the additional pharmaceutical agent is nab-paclitaxel. In some embodiments, the additional pharmaceutical agent is necitumumab. In some embodiments, the additional pharmaceutical agent is nivolumab. In some embodiments, the additional pharmaceutical agent is osimertinib. In some embodiments, the additional pharmaceutical agent is osimertinib mesylate. In some embodiments, the additional pharmaceutical agent is paclitaxel. In some embodiments, the additional pharmaceutical agent is paclitaxel albumin-stabilized nanoparticle formulation. In some embodiments, the additional pharmaceutical agent is pembrolizumab. In some embodiments, the additional pharmaceutical agent is pemetrexed. In some embodiments, the additional pharmaceutical agent is pemetrexed disodium. In some embodiments, the additional pharmaceutical agent is pralsetinib. In some embodiments, the additional pharmaceutical agent is ramucirumab. In some embodiments, the additional pharmaceutical agent is rinotecan hydrochloride. In some embodiments, the additional pharmaceutical agent is selpercatinib. In some embodiments, the additional pharmaceutical agent is tepotinib. In some embodiments, the additional pharmaceutical agent is tepotinib hydrochloride. In some embodiments, the additional pharmaceutical agent is topotecan. In some embodiments, the additional pharmaceutical agent is topotecan hydrochloride. In some embodiments, the additional pharmaceutical agent is trametinib. In some embodiments, the additional pharmaceutical agent is trametinib dimethyl sulfoxide. In some embodiments, the additional pharmaceutical agent is vincristine sulfate. In some embodiments, the additional pharmaceutical agent is vinorelbine. In some embodiments, the additional pharmaceutical agent is vinorelbine tartrate.
[0415] In some embodiments, the additional pharmaceutical agent is ibrutinib. In some embodiments, the additional pharmaceutical agent is idelalisib. In some embodiments, the additional pharmaceutical agent is lenalidomide. In some embodiments, the additional pharmaceutical agent is venetoclax. In some embodiments, the additional pharmaceutical agent is FLT3 inhibitor. In some embodiments, the additional pharmaceutical agent is IDH 1/2 inhibitor. In some embodiments, the additional pharmaceutical agent is glasdegib. In some embodiments, the additional pharmaceutical agent is azacytidine. In some embodiments, the additional pharmaceutical agent is cyclophosphamide. In some embodiments, the additional pharmaceutical agent is decitabine. In some embodiments, the additional pharmaceutical agent is enasidenib. In some embodiments, the additional pharmaceutical agent is erastin. In some embodiments, the additional pharmaceutical agent is gilterinib. In some embodiments, the additional pharmaceutical agent is idasanutlin. In some embodiments, the additional pharmaceutical agent is ivosidenib. In some embodiments, the additional pharmaceutical agent is ixazomib. In some embodiments, the additional pharmaceutical agent is midostaurin. In some embodiments, the additional pharmaceutical agent is navitoclax. In some embodiments, the additional pharmaceutical agent is onvasertib. In some embodiments, the additional pharmaceutical agent is cyclosporin A. In some embodiments, the additional pharmaceutical agent is pacritinib. In some embodiments, the additional pharmaceutical agent is phorbol 12-myristate 13-acetate (PMA). In some embodiments, the additional pharmaceutical agent is ruxolitinib. In some embodiments, the additional pharmaceutical agent is S055746. In some embodiments, the additional pharmaceutical agent is selinexor (KPT-330). In some embodiments, the additional pharmaceutical agent is trametinib. In some embodiments, the additional pharmaceutical agent is tretinoin. In some embodiments, the additional pharmaceutical agent is etoposide. In some embodiments, the additional pharmaceutical agent is P7C3. In some embodiments, the additional pharmaceutical agent is napabucasin. In some embodiments, the additional pharmaceutical agent is olaparib. In some embodiments, the additional pharmaceutical agent is AraC. In some embodiments, the additional pharmaceutical agent is daunorubicin. In some embodiments, the additional pharmaceutical agent is l-methyl-3-nitro-l- nitrosoguanidinium (MNNG). In some embodiments, the additional pharmaceutical agent is melphalan. In some embodiments, the additional pharmaceutical agent is verapamil. In some embodiments, the additional pharmaceutical agent is etoposide. In some embodiments, the additional pharmaceutical agent is cisplatin. In some embodiments, the additional pharmaceutical agent is anti- PD1. In some embodiments, the additional pharmaceutical agent is tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In some embodiments, the additional pharmaceutical agent is EX527. In some embodiments, the additional pharmaceutical agent is sirtinol. In some embodiments, the additional pharmaceutical agent is cambinol. In some embodiments, the additional pharmaceutical agent is vorinostat. In some embodiments, the additional pharmaceutical agent is valproic acid. In some embodiments, the additional pharmaceutical agent is butyrate. In some embodiments, the additional pharmaceutical agent is JPH203. In some embodiments, the additional pharmaceutical agent is L-asparaginase. In some embodiments, the additional pharmaceutical agent is bortezomib. In some embodiments, the additional pharmaceutical agent is rituximab. In some embodiments, the additional pharmaceutical agent is cyclosporin-A. In some embodiments, the additional pharmaceutical agent is PGP-4008. In some embodiments, the additional pharmaceutical agent is β- lapachone. In some embodiments, the additional pharmaceutical agent is β-methylene adenosine 5′- diphosphate (APCP). In some embodiments, the additional pharmaceutical agent is gemcitabine. In some embodiments, the additional pharmaceutical agent is Lu-DOTATATE. In some embodiments, the additional pharmaceutical agent is fluorouracil (5-FU). In some embodiments, the additional pharmaceutical agent is pemetrexed. In some embodiments, the additional pharmaceutical agent is temozolomide. In some embodiments, the additional pharmaceutical agent is onvansertib. In some embodiments, the additional pharmaceutical agent is cedazuridine. In some embodiments, the additional pharmaceutical agent is galinpepimut-S. In some embodiments, the additional pharmaceutical agent is cedazuridine/decitabine. In some embodiments, the additional pharmaceutical agent is cytarabine/daunorubicin. In some embodiments, the additional pharmaceutical agent is uproleselan. In some embodiments, the additional pharmaceutical agent is gedatolisib. In some embodiments, the additional pharmaceutical agent is devimistat. In some embodiments, the additional pharmaceutical agent is glasdegib. In some embodiments, the additional pharmaceutical agent is idasanutlin. In some embodiments, the additional pharmaceutical agent is ganetespib. In some embodiments, the additional pharmaceutical agent is tipifarnib. In some embodiments, the additional pharmaceutical agent is midostaurin. In some embodiments, the additional pharmaceutical agent is pevonedistat. In some embodiments, the additional pharmaceutical agent is ivosidenib. In some embodiments, the additional pharmaceutical agent is crenolanib. In some embodiments, the additional pharmaceutical agent is quizartinib. In some embodiments, the additional pharmaceutical agent is pracinostat. In some embodiments, the additional pharmaceutical agent is guadecitabine. In some embodiments, the additional pharmaceutical agent is DFP 10917. In some embodiments, the additional pharmaceutical agent is vosaroxin. In some embodiments, the additional pharmaceutical agent is rexlemestrocel-L-mesoblast. In some embodiments, the additional pharmaceutical agent is treosulfan. In some embodiments, the additional pharmaceutical agent is sapacitabine. In some embodiments, the additional pharmaceutical agent is enasidenib. In some embodiments, the additional pharmaceutical agent is volasertib [0416] In some embodiments, the additional pharmaceutical agent is selected from the group consisting of pentostatin, pravastatin, marimastat, cladribine, zidovudine, arsenic trioxide, pegaspargase, filgrastim (G-CSF), pegfilgrastim (PEGylated G-CSF), lipegfilgrastim (glycopegylated), PEG-rhG-CSF, BBR2778 (pixantrone), yangzhengxiaoji capsule, enzastaurin, alisertib (MLN8237, aurora A kinase inhibitor), zidovudine, amrubicin, palifosfamide-tris, picoplatin, BCG vaccine, BEC2 vaccine, belotecan, meclinertant (SR48692), nelarabine, pralatrexate, plerixafor, and an additional pharmaceutical agent selected from proton pump inhibitors; alkylating additional pharmaceutical agents (e.g., bendamustine, fotemustine); BTK inhibitors (e.g., LOXO-305 (pirtobrutinib), Orelabrutinib (ICP-022), acalabrutinib, zanubrutinib); HDAC inhibitors (e.g., chidamide, panobinostat (LBH589), belinostat, romidepsin); rituximab and biosimilars thereof (e.g., ABP798, GP2013, CT-P10, RTXM83, MabThera, HLX01, TQB2303, MabionCD20 ® , JHL1101, BI695500, IBI301, CT-P10 (Truxima ® ), SIBP-02, SAIT101); VEGFR inhibitors (e.g., AL3810 (lucitanib), apatinib, chiauranib, anlotinib hydrochloride); PI3K inhibitors (e.g., copanlisib (BAY 80- 6946), zandelisib (ME-401), parsaclisib (PI3K inhibitor), duvelisib, umbralisib); EZH2 inhibitors (e.g., tazemetostat); IL-2 fusion toxin (e.g., E7777, ontak (denileukin diftitox)); cell based gene therapies (e.g., axicabtagene ciloleucel, brexucabtagene autoleucel, lisocabtagene maraleucel, tisagenlecleucel); or antibodies (e.g., HuMax-CD4, anti CD20 therapies (e.g., ofatumumab, MIL62, ublituximab (TGTX-1101), obinutuzumab, ibritumomab tiuxetan (Zevalin ® )), anti CD3/CD20 therapies (e.g., epcoritamab, mosunetuzumab, glofitamab, blinatumomab), anti CD19 therapies (e.g., tafasitamab, loncastuximab tesirine), anti CD22 therapies (e.g., epratuzumab, inotuzumab ozogamicin), PD-1 antibodies (e.g., toripalimab, HLX10, islelizumab, SHR1316, tislelizumab, camrelizumab (SHR-1210), sintilimab, TQB2450), anti CD137 (e.g., utomilumab), anti CD80 (e.g., galiximab), anti CCR4 (e.g., KW-0761 (mogamulizumab)), anti CD25 (e.g. daclizumab), anti CD319 (e.g., elotuzumab), anti CTLA-4 (e.g., tremelimumab), anti DLL3 (e.g., ovalpitumumab tesirine), anti GD2 (e.g., dinutuximab), anti CD30 (e.g., brentuximab vedotin), anti CD79B (e.g., polatuzumab vedotin), anti TIGIT (e.g., tiragolumab)). [0417] In some embodiments, the additional pharmaceutical agent is pentostatin. In some embodiments, the additional pharmaceutical agent is pravastatin. In some embodiments, the additional pharmaceutical agent is marimastat. In some embodiments, the additional pharmaceutical agent is cladribine. In some embodiments, the additional pharmaceutical agent is zidovudine. In some embodiments, the additional pharmaceutical agent is arsenic trioxide. In some embodiments, the additional pharmaceutical agent is pegaspargase. In some embodiments, the additional pharmaceutical agent is filgrastim (G-CSF). In some embodiments, the additional pharmaceutical agent is pegfilgrastim (PEGylated G-CSF). In some embodiments, the additional pharmaceutical agent is lipegfilgrastim (glycopegylated). In some embodiments, the additional pharmaceutical agent is PEG- rhG-CSF. In some embodiments, the additional pharmaceutical agent is BBR2778 (pixantrone). In some embodiments, the additional pharmaceutical agent is yangzhengxiaoji capsule. In some embodiments, the additional pharmaceutical agent is enzastaurin. In some embodiments, the additional pharmaceutical agent is alisertib (MLN8237). In some embodiments, the additional pharmaceutical agent is an aurora A kinase inhibitor. In some embodiments, the additional pharmaceutical agent is zidovudine. In some embodiments, the additional pharmaceutical agent is amrubicin. In some embodiments, the additional pharmaceutical agent is palifosfamide-tris. In some embodiments, the additional pharmaceutical agent is picoplatin. In some embodiments, the additional pharmaceutical agent is a BCG vaccine. In some embodiments, the additional pharmaceutical agent is a BEC2 vaccine. In some embodiments, the additional pharmaceutical agent is belotecan. In some embodiments, the additional pharmaceutical agent is meclinertant (SR48692). In some embodiments, the additional pharmaceutical agent is nelarabine. In some embodiments, the additional pharmaceutical agent is pralatrexate. In some embodiments, the additional pharmaceutical agent is plerixafor. In some embodiments, the additional pharmaceutical agent is a proton pump inhibitor. In some embodiments, the additional pharmaceutical agent is an alkylating additional pharmaceutical agent. In some embodiments, the additional pharmaceutical agent is bendamustine. In some embodiments, the additional pharmaceutical agent is fotemustine. In some embodiments, the additional pharmaceutical agent is a BTK inhibitor. In some embodiments, the additional pharmaceutical agent is LOXO-305 (pirtobrutinib). In some embodiments, the additional pharmaceutical agent is Orelabrutinib (ICP-022). In some embodiments, the additional pharmaceutical agent is acalabrutinib. In some embodiments, the additional pharmaceutical agent is zanubrutinib. In some embodiments, the additional pharmaceutical agent is a HDAC inhibitor. In some embodiments, the additional pharmaceutical agent is chidamide. In some embodiments, the additional pharmaceutical agent is panobinostat (LBH589). In some embodiments, the additional pharmaceutical agent is belinostat. In some embodiments, the additional pharmaceutical agent is romidepsin. In some embodiments, the additional pharmaceutical agent is rituximab. In some embodiments, the additional pharmaceutical agent is rituximab or a biosimilar thereof. In some embodiments, the additional pharmaceutical agent is ABP798. In some embodiments, the additional pharmaceutical agent is GP2013. In some embodiments, the additional pharmaceutical agent is CT- P10. In some embodiments, the additional pharmaceutical agent is RTXM83. In some embodiments, the additional pharmaceutical agent is MabThera. In some embodiments, the additional pharmaceutical agent is HLX01. In some embodiments, the additional pharmaceutical agent is TQB2303. In some embodiments, the additional pharmaceutical agent is MabionCD20®. In some embodiments, the additional pharmaceutical agent is JHL1101. In some embodiments, the additional pharmaceutical agent is BI695500. In some embodiments, the additional pharmaceutical agent is IBI301. In some embodiments, the additional pharmaceutical agent is CT-P10 (Truxima®). In some embodiments, the additional pharmaceutical agent is SIBP-02. In some embodiments, the additional pharmaceutical agent is SAIT101. In some embodiments, the additional pharmaceutical agent is a VEGFR inhibitor. In some embodiments, the additional pharmaceutical agent is AL3810 (lucitanib). In some embodiments, the additional pharmaceutical agent is apatinib. In some embodiments, the additional pharmaceutical agent is chiauranib. In some embodiments, the additional pharmaceutical agent is anlotinib hydrochloride. In some embodiments, the additional pharmaceutical agent is a PI3K inhibitor . In some embodiments, the additional pharmaceutical agent is copanlisib (BAY 80-6946). In some embodiments, the additional pharmaceutical agent is zandelisib (ME-401). In some embodiments, the additional pharmaceutical agent is parsaclisib. In some embodiments, the additional pharmaceutical agent is a PI3K inhibitor. In some embodiments, the additional pharmaceutical agent is duvelisib. In some embodiments, the additional pharmaceutical agent is umbralisib. In some embodiments, the additional pharmaceutical agent is a EZH2 inhibitor. In some embodiments, the additional pharmaceutical agent is tazemetostat. In some embodiments, the additional pharmaceutical agent is a IL-2 fusion toxin. In some embodiments, the additional pharmaceutical agent is E7777. In some embodiments, the additional pharmaceutical agent is ontak (denileukin diftitox). In some embodiments, the additional pharmaceutical agent is a cell based gene therapy. In some embodiments, the additional pharmaceutical agent is axicabtagene ciloleucel. In some embodiments, the additional pharmaceutical agent is brexucabtagene autoleucel. In some embodiments, the additional pharmaceutical agent is lisocabtagene maraleucel. In some embodiments, the additional pharmaceutical agent is tisagenlecleucel. In some embodiments, the additional pharmaceutical agent is an antibody. In some embodiments, the additional pharmaceutical agent is HuMax-CD4. In some embodiments, the additional pharmaceutical agent is an anti CD20 therapy . In some embodiments, the additional pharmaceutical agent is ofatumumab. In some embodiments, the additional pharmaceutical agent is MIL62. In some embodiments, the additional pharmaceutical agent is ublituximab (TGTX-1101). In some embodiments, the additional pharmaceutical agent is obinutuzumab. In some embodiments, the additional pharmaceutical agent is ibritumomab tiuxetan (Zevalin®). In some embodiments, the additional pharmaceutical agent is an anti CD3/CD20 therapy. In some embodiments, the additional pharmaceutical agent is epcoritamab. In some embodiments, the additional pharmaceutical agent is mosunetuzumab. In some embodiments, the additional pharmaceutical agent is glofitamab. In some embodiments, the additional pharmaceutical agent is blinatumomab. In some embodiments, the additional pharmaceutical agent is an anti CD19 therapy. In some embodiments, the additional pharmaceutical agent is tafasitamab. In some embodiments, the additional pharmaceutical agent is loncastuximab tesirine. In some embodiments, the additional pharmaceutical agent is an anti CD22 therapy. In some embodiments, the additional pharmaceutical agent is epratuzumab. In some embodiments, the additional pharmaceutical agent is inotuzumab ozogamicin. In some embodiments, the additional pharmaceutical agent is a PD-1 antibody. In some embodiments, the additional pharmaceutical agent is toripalimab. In some embodiments, the additional pharmaceutical agent is HLX10. In some embodiments, the additional pharmaceutical agent is islelizumab. In some embodiments, the additional pharmaceutical agent is SHR1316. In some embodiments, the additional pharmaceutical agent is tislelizumab. In some embodiments, the additional pharmaceutical agent is camrelizumab (SHR-1210). In some embodiments, the additional pharmaceutical agent is sintilimab. In some embodiments, the additional pharmaceutical agent is TQB2450. In some embodiments, the additional pharmaceutical agent is an anti CD137 therapy. In some embodiments, the additional pharmaceutical agent is utomilumab. In some embodiments, the additional pharmaceutical agent is an anti CD80 therapy. In some embodiments, the additional pharmaceutical agent is galiximab. In some embodiments, the additional pharmaceutical agent is an anti CCR4 therapy. In some embodiments, the additional pharmaceutical agent is KW-0761 (mogamulizumab). In some embodiments, the additional pharmaceutical agent is an anti CD25 therapy. In some embodiments, the additional pharmaceutical agent is daclizumab. In some embodiments, the additional pharmaceutical agent is an anti CD319 therapy. In some embodiments, the additional pharmaceutical agent is elotuzumab. In some embodiments, the additional pharmaceutical agent is an anti CTLA-4 therapy. In some embodiments, the additional pharmaceutical agent is tremelimumab. In some embodiments, the additional pharmaceutical agent is an anti DLL3 therapy. In some embodiments, the additional pharmaceutical agent is ovalpitumumab tesirine. In some embodiments, the additional pharmaceutical agent is an anti GD2 therapy. In some embodiments, the additional pharmaceutical agent is dinutuximab. In some embodiments, the additional pharmaceutical agent is an anti CD30 therapy. In some embodiments, the additional pharmaceutical agent is brentuximab vedotin. In some embodiments, the additional pharmaceutical agent is an anti CD79B therapy. In some embodiments, the additional pharmaceutical agent is polatuzumab vedotin. In some embodiments, the additional pharmaceutical agent is an anti TIGIT therapy. In some embodiments, the additional pharmaceutical agent is tiragolumab. [0418] In some embodiments, the additional pharmaceutical agent is an ophthalmic agent. In some embodiments, the additional pharmaceutical agent is selected from the group consisting of 15-HETE, AdPEDF, anecortave acetate, brimonidine, cevimeline, combretatstatin, cyclosporine A, denufosol, dexamethamethasone, diquafosol, estradiol, hyaluronidase, latanoprost, memantine, NT-501, octreotide, pegaptanib sodium, pilocarpine, ranibizumab, rostaporfin, ruboxistaurin, squalamine, tacrolimus, and verteporfin. In some embodiments, the additional pharmaceutical agent is selected from 15-HETE, Restasis ® , Androgen Tear ® , INS365 ® , OPC-12759 ® , FK-506 ® , Retaane ® , Macugen ® , RhuFab ® , SnET2 ® , and Visudyne ® . In some embodiments, the additional pharmaceutical agent is selected from voretigene neparvovec, hypertonic sodium chloride, riboflavin, ocriplasmin, mitomycin, dapiprazole, cysteamine, cenegermin, sodium hyaluronate, sodium chondroitin sulfate, ketorolac, phenylephrine, latanoprost, travoprost, bimatoprost, tafluprost, unoprostone isopropyl, brimonidine, apraclonidine, timolol, betaxolol, carteolol, levobunolol, dorzolamide, brinzolamide, acetazolamide, carbachol, and pilocarpine. [0419] In some embodiments, the additional pharmaceutical agent is 15-HETE. In some embodiments, the additional pharmaceutical agent is AdPEDF. In some embodiments, the additional pharmaceutical agent is anecortave acetate. In some embodiments, the additional pharmaceutical agent is brimonidine. In some embodiments, the additional pharmaceutical agent is cevimeline. In some embodiments, the additional pharmaceutical agent is combretatstatin. In some embodiments, the additional pharmaceutical agent is cyclosporine A. In some embodiments, the additional pharmaceutical agent is denufosol. In some embodiments, the additional pharmaceutical agent is dexamethamethasone. In some embodiments, the additional pharmaceutical agent is diquafosol. In some embodiments, the additional pharmaceutical agent is estradiol. In some embodiments, the additional pharmaceutical agent is hyaluronidase. In some embodiments, the additional pharmaceutical agent is latanoprost. In some embodiments, the additional pharmaceutical agent is memantine. In some embodiments, the additional pharmaceutical agent is NT-501. In some embodiments, the additional pharmaceutical agent is octreotide. In some embodiments, the additional pharmaceutical agent is pegaptanib sodium. In some embodiments, the additional pharmaceutical agent is pilocarpine. In some embodiments, the additional pharmaceutical agent is ranibizumab. In some embodiments, the additional pharmaceutical agent is rostaporfin. In some embodiments, the additional pharmaceutical agent is ruboxistaurin. In some embodiments, the additional pharmaceutical agent is squalamine. In some embodiments, the additional pharmaceutical agent is tacrolimus. In some embodiments, the additional pharmaceutical agent is verteporfin. In some embodiments, the additional pharmaceutical agent is 15-HETE. In some embodiments, the additional pharmaceutical agent is Restasis ® . In some embodiments, the additional pharmaceutical agent is Androgen Tear ® . In some embodiments, the additional pharmaceutical agent is INS365 ® . In some embodiments, the additional pharmaceutical agent is OPC-12759 ® . In some embodiments, the additional pharmaceutical agent is FK-506 ® . In some embodiments, the additional pharmaceutical agent is Retaane ® . In some embodiments, the additional pharmaceutical agent is Macugen ® . In some embodiments, the additional pharmaceutical agent is RhuFab ® . In some embodiments, the additional pharmaceutical agent is SnET2 ® . In some embodiments, the additional pharmaceutical agent is Visudyne ® . In some embodiments, the additional pharmaceutical agent is voretigene. In some embodiments, the additional pharmaceutical agent is neparvovec. In some embodiments, the additional pharmaceutical agent is hypertonic sodium chloride. In some embodiments, the additional pharmaceutical agent is riboflavin. In some embodiments, the additional pharmaceutical agent is ocriplasmin. In some embodiments, the additional pharmaceutical agent is mitomycin. In some embodiments, the additional pharmaceutical agent is dapiprazole. In some embodiments, the additional pharmaceutical agent is cysteamine. In some embodiments, the additional pharmaceutical agent is cenegermin. In some embodiments, the additional pharmaceutical agent is sodium hyaluronate. In some embodiments, the additional pharmaceutical agent is sodium chondroitin sulfate. In some embodiments, the additional pharmaceutical agent is ketorolac. In some embodiments, the additional pharmaceutical agent is phenylephrine. In some embodiments, the additional pharmaceutical agent is latanoprost. In some embodiments, the additional pharmaceutical agent is travoprost. In some embodiments, the additional pharmaceutical agent is bimatoprost. In some embodiments, the additional pharmaceutical agent is tafluprost. In some embodiments, the additional pharmaceutical agent is unoprostone isopropyl. In some embodiments, the additional pharmaceutical agent is brimonidine. In some embodiments, the additional pharmaceutical agent is apraclonidine. In some embodiments, the additional pharmaceutical agent is timolol. In some embodiments, the additional pharmaceutical agent is betaxolol. In some embodiments, the additional pharmaceutical agent is carteolol. In some embodiments, the additional pharmaceutical agent is levobunolol. In some embodiments, the additional pharmaceutical agent is dorzolamide. In some embodiments, the additional pharmaceutical agent is brinzolamide. In some embodiments, the additional pharmaceutical agent is acetazolamide. In some embodiments, the additional pharmaceutical agent is carbachol. In some embodiments, the additional pharmaceutical agent is pilocarpine.
[0420] In some embodiments, the immunotherapy may involve one of more of the following steps: preventing or inhibiting the growth of cancer cells; preventing cancer from spreading to other parts of the body; and improving the ability and activity of the immune system to kill cancer cells. Nonlimiting examples of immunotherapies include: monoclonal antibodies, checkpoint inhibitors, nonspecific immunotherapies, oncolytic virus therapy, T cell therapies, and cancer vaccines.
[0421] In certain embodiments, the immunotherapy utilizes monoclonal antibodies. In some embodiments, the monoclonal antibodies target (bind to) and/or block an abnormal protein on a cancer cell.
[0422] In certain embodiments, the immunotherapy includes immunotherapeutic strategies for leukemia. In some embodiments, the immunotherapy includes vaccination with leukemia-associated antigens. In certain embodiments, the immunotherapy includes adoptive transfer of allogeneic natural killer cells.
[0423] In certain embodiments, the immunotherapy utilizes checkpoint inhibitors. In certain embodiments, the immune checkpoint inhibitors are monoclonal antibodies. Immune checkpoints are regulators of immune activation by maintaining immune homeostasis and preventing autoimmunity. In cancer cells, immune checkpoint mechanisms are often activated to suppress the nascent anticancer immune response. In some embodiments, the checkpoint inhibitor is an inhibitor of PD-1 (programmed cell death protein 1). In some embodiments, the checkpoint inhibitor is an inhibitor of PD-L1 (programmed death-ligand 1). In some embodiments, the checkpoint inhibitor is an inhibitor of CTLA-4 (cytotoxic T-lymphocyte-associated protein 4). Examples of immune checkpoint inhibitors include, without limitation, Ipilimumab (Yervoy), Nivolumab (Opdivo), Pembrolizumab (Keytruda), Atezolizumab (Tecentriq), Avelumab (Bavencio), and Durvalumab (Imfinzi).
[0424] In certain embodiments, the immunotherapy is non-specific immunotherapy (e.g., interferons or interleukins). In certain embodiments, the immunotherapy is an oncolytic virus therapy.
[0425] In certain embodiments, the immunotherapy is a T cell therapy. In some embodiments, the T cell therapy is chimeric antigen receptor (CAR) T cell therapy.
[0426] In certain embodiments, the immunotherapy is an anti- cancer vaccine.
[0427] Anti-cancer agents encompass biotherapeutic anti-cancer agents as well as chemotherapeutic agents. Exemplary biotherapeutic anti-cancer agents include, but are not limited to, interferons, cytokines (e.g., tumor necrosis factor, interferon α, interferon γ), vaccines, hematopoietic growth factors, monoclonal serotherapy, immunostimulants and/or immunomodulatory agents (e.g., IL-1, 2, 4, 6, or 12), immune cell growth factors (e.g., GM-CSF) and antibodies (e.g. Herceptin (trastuzumab), T-DM1, AVASTIN (bevacizumab), ERBITUX (cetuximab), Vectibix (panitumumab), Rituxan (rituximab), Bexxar (tositumomab)). Exemplary chemotherapeutic agents include, but are not limited to, anti-estrogens (e.g. tamoxifen, raloxifene, and megestrol), LHRH agonists (e.g. goserelin and leuprolide), anti-androgens (e.g. flutamide and bicalutamide), photodynamic therapies (e.g. vertoporfin (BPD-MA), phthalocyanine, photosensitizer Pc4, and demethoxy-hypocrellin A (2BA-2- DMHA)), nitrogen mustards (e.g. cyclophosphamide, ifosfamide, trofosfamide, chlorambucil, estramustine, and melphalan), nitrosoureas (e.g. carmustine (BCNU) and lomustine (CCNU)), alkylsulphonates (e.g. busulfan and treosulfan), triazenes (e.g. dacarbazine, temozolomide), platinum containing compounds (e.g. cisplatin, carboplatin, oxaliplatin), vinca alkaloids (e.g. vincristine, vinblastine, vindesine, and vinorelbine), taxoids (e.g. paclitaxel or a paclitaxel equivalent) docosahexaenoic acid bound-paclitaxel (DHA-paclitaxel, Taxoprexin), polyglutamate bound- paclitaxel (PG-paclitaxel, paclitaxel poliglumex, CT-2103, XYOTAX), the tumor-activated prodrug (TAP) ANG1005 (Angiopep-2 bound to three molecules of paclitaxel), paclitaxel-EC-1 (paclitaxel bound to the erbB2-recognizing peptide EC-1), paclitaxel albumin-stabilized nanoparticle formulation, and glucose-conjugated paclitaxel, e.g., 2'-paclitaxel methyl 2-glucopyranosyl succinate; docetaxel, taxol), epipodophyllins (e.g., etoposide, etoposide phosphate, teniposide, topotecan, 9- aminocamptothecin, camptoirinotecan, irinotecan, crisnatol, mytomycin C), anti-metabolites, DHFR inhibitors (e.g., methotrexate, dichloromethotrexate, trimetrexate, edatrexate), IMP dehydrogenase inhibitors (e.g., mycophenolic acid, tiazofurin, ribavirin, and EICAR), ribonuclotide reductase inhibitors (e.g. hydroxyurea and deferoxamine), uracil analogs (e.g., 5-fluorouracil (5-FU), floxuridine, doxifluridine, ratitrexed, tegafur-uracil, capecitabine), cytosine analogs (e.g., cytarabine (ara C), cytosine arabinoside, and fludarabine), purine analogs (e.g., mercaptopurine and Thioguanine), Vitamin D3 analogs (e.g., EB 1089, CB 1093, and KH 1060), isoprenylation inhibitors (e.g., lovastatin), dopaminergic neurotoxins (e.g.1-methyl-4-phenylpyridinium ion), cell cycle inhibitors (e.g., staurosporine), actinomycin (e.g. actinomycin D, dactinomycin), bleomycin (e.g., bleomycin A2, bleomycin B2, peplomycin), anthracycline (e.g., daunorubicin, doxorubicin, pegylated liposomal doxorubicin, idarubicin, epirubicin, pirarubicin, zorubicin, mitoxantrone), MDR inhibitors (e.g., verapamil), Ca 2+ ATPase inhibitors (e.g., thapsigargin), imatinib, thalidomide, lenalidomide, tyrosine kinase inhibitors (e.g., axitinib (AG013736), bosutinib (SKI-606), cediranib (RECENTIN TM , AZD2171), dasatinib (SPRYCEL ® , BMS-354825), erlotinib (TARCEVA ® ), gefitinib (IRESSA ® ), imatinib (Gleevec ® , CGP57148B, STI-571), lapatinib (TYKERB ® , TYVERB ® ), lestaurtinib (CEP- 701), neratinib (HKI-272), nilotinib (TASIGNA ® ), semaxanib (semaxinib, SU5416), sunitinib (SUTENT ® , SU11248), toceranib (PALLADIA ® ), vandetanib (ZACTIMA ® , ZD6474), vatalanib (PTK787, PTK/ZK), trastuzumab (HERCEPTIN ® ), bevacizumab (AVASTIN ® ), rituximab (RITUXAN ® ), cetuximab (ERBITUX ® ), panitumumab (VECTIBIX ® ), ranibizumab (Lucentis ® ), nilotinib (TASIGNA ® ), sorafenib (NEXAVAR ® ), everolimus (AFINITOR ® ), alemtuzumab (CAMPATH ® ), gemtuzumab ozogamicin (MYLOTARG ® ), temsirolimus (TORISEL ® ), ENMD-2076, PCI-32765, AC220, dovitinib lactate (TKI258, CHIR-258), BIBW 2992 (TOVOK TM ), SGX523, PF- 04217903, PF-02341066, PF-299804, BMS-777607, ABT-869, MP470, BIBF 1120 (VARGATEF ® ), AP24534, JNJ-26483327, MGCD265, DCC-2036, BMS-690154, CEP-11981, tivozanib (AV-951), OSI-930, MM-121, XL-184, XL-647, and/or XL228), proteasome inhibitors (e.g., bortezomib (Velcade)), mTOR inhibitors (e.g., rapamycin, temsirolimus (CCI-779), everolimus (RAD-001), ridaforolimus, AP23573 (Ariad), AZD8055 (AstraZeneca), BEZ235 (Novartis), BGT226 (Novartis), XL765 (Sanofi Aventis), PF-4691502 (Pfizer), GDC0980 (Genetech), SF1126 (Semafoe), and OSI- 027 (OSI)), oblimersen, gemcitabine, carminomycin, leucovorin, pemetrexed, cyclophosphamide, dacarbazine, procarbizine, prednisolone, dexamethasone, campathecin, plicamycin, asparaginase, aminopterin, methopterin, porfiromycin, melphalan, leurosidine, leurosine, chlorambucil, trabectedin, procarbazine, discodermolide, carminomycin, aminopterin, and hexamethyl melamine. [0428] In some embodiments, the compound or composition is substantially soluble in water (e.g., hydrophilic). In some embodiments, the compound or composition is substantially insoluble in water (e.g., hydrophobic). In some embodiments, the compound or composition is substantially insoluble in water and greater than about 10,000 parts water are required to dissolve 1 part compound of the disclosure. [0429] In some embodiments, the percentage of the composition that comprises a compound of the disclosure is between about 1 and about 100% (e.g., about 1%, about 2%, about 3%, about 4%, about 5%, about 10%, about 15%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, or about 100%). In some embodiments, the percentage of the composition that comprise a compound of the disclosure is less than about 50%, e.g., less than about 40%, less than about 35%, less than about 30%, less than about 25%, less than about 20%, less than about 15%, or less than about 10%. In some embodiments, the percentage of the composition that comprise a compound of the disclosure is between about 5% and about 50%, about 5% and about 40%, about 5% and about 30%, about 5% and about 25%, or about 5% and about 20%. In some embodiments, the percentage of the composition that comprise a compound of the disclosure is between about 5% and 90%. In some embodiments, the percentage of the composition that comprise a compound of the disclosure is between about 5% and about 75%. In some embodiments, the composition that comprise a compound of the disclosure is between about 5% and about 50%. In some embodiments, the percentage of the composition that comprise a compound of the disclosure is between about 10% and about 25%. [0430] In some embodiments, the total amount of the compound of the disclosure present in the composition is greater than about 1% (e.g., about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 12%, about 15%, about 20%, about 25%, about 30%, or more) of the total size or weight of the composition. In some embodiments, the total amount of the compound of the disclosure present in the composition is greater than about 10% (e.g., about 12%, about 15%, about 20%, about 25%, about 30%, or more) of the total size or weight of the composition. [0431] In some embodiments, the compound of the disclosure is incorporated into a composition at a dose that is less than the dose or amount of said compound in free form to have a desired effect (e.g., a desired therapeutic effect). In certain embodiments, the composition increases the amount of the compound of the disclosure delivered to a tissue or cell in need thereof and reduces the amount of the compound of the disclosure exposed to a non-target tissue or cell, as compared to the free compound. [0432] In another aspect, provided are kits comprising a compound of the disclosure; or a pharmaceutical composition as described herein; and instructions for using the compound of the disclosure or pharmaceutical composition of the disclosure. [0433] In some embodiments, the kits further comprise an additional pharmaceutical agent as described herein. In certain embodiments, the kits further comprise instructions regarding the order of use of the compound of the disclosure or composition of the disclosure and the additional pharmaceutical agent. In some embodiments, the instructions provide that a compound of the disclosure or composition of the disclosure is administered before the additional pharmaceutical agent. In some embodiments, the instructions provide that a compound of the disclosure or composition of the disclosure is administered concurrently with the additional pharmaceutical agent. In some embodiments, the instructions provide that a compound of the disclosure or composition of the disclosure is administered after the additional pharmaceutical agent. [0434] In certain embodiments, the instructions of the kit may also include information as required by a regulatory agency such as the U.S. Food and Drug Administration (FDA). In certain embodiments, the information included in the kits is prescribing information. In certain embodiments, the kits and instructions provide for delivering a compound of the disclosure. In certain embodiments, the kits and instructions provide for delivering a composition. In certain embodiments, the kits and instructions provide for treating cancer in a subject in need thereof. In certain embodiments, the kits and instructions provide for inhibiting the signaling pathway in a subject or cell. Methods of Treatment and Prevention and Uses [0435] Also provided herein are methods and uses for treating or preventing any disease as described herein using a compound or composition as described herein. [0436] In some embodiments, the present disclosure provides methods for treating cancer comprising administering to a subject a therapeutically effective amount of a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein the variables recited in Formula (I), (II), (III), or (IV) are as described herein. [0437] In certain embodiments, the cancer comprises cancer stem cells. In certain embodiments, the cancer involves or is associated with cancer stem cells. In certain embodiments, the cancer is colorectal cancer, gastric cancer, gastrointestinal stromal tumor, ovarian cancer, lung cancer, breast cancer, pancreatic cancer, testicular cancer, prostate cancer, liver cancer, or endometrial cancer. In certain embodiments, the cancer is leukemia (e.g., acute myeloid leukemia). In certain embodiments, the cancer is lymphoma. In certain embodiments, the cancer is multiple myeloma. In certain embodiments, the subject is in need of regenerative medicine or therapy. [0438] In yet another aspect, the present disclosure provides methods and uses comprising contacting a cell with an effective amount of a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0439] In certain aspects, the present disclosure provides methods and uses comprising killing a cell with an effective amount of a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0440] In certain embodiments, the present disclosure provides methods of modulating NAMPT in a subject comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting NAMPT. In some embodiments, the modulating is activating NAMPT. [0441] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating NAMPT in a subject comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting NAMPT. In some embodiments, the modulating is activating NAMPT. [0442] In certain embodiments, the present disclosure provides methods of inhibiting NAMPT in a subject comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0443] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by inhibiting NAMPT in a subject comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0444] In certain embodiments, the present disclosure provides methods of modulating inflammatory activity in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting inflammatory activity. [0445] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating inflammatory activity in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof In some embodiments, the modulating is inhibiting inflammatory activity. [0446] In certain embodiments, the present disclosure provides methods of decreasing inflammatory activity in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0447] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by decreasing inflammatory activity in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof [0448] In certain embodiments, the present disclosure provides methods of modulating cellular metabolism in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting or decreasing cellular metabolism. In some embodiments, the modulating is activating or increasing cellular metabolism. [0449] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating cellular metabolism in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting or decreasing cellular metabolism. In some embodiments, the modulating is activating or increasing cellular metabolism. [0450] In certain embodiments, the present disclosure provides methods of decreasing cellular metabolism in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0451] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by decreasing cellular metabolism in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0452] In certain embodiments, the present disclosure provides methods of modulating cellular metabolic activity in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting or decreasing cellular metabolic activity. In some embodiments, the modulating is activating or increasing cellular metabolic activity. [0453] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating cellular metabolic activity in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting or decreasing cellular metabolic activity. In some embodiments, the modulating is activating or increasing cellular metabolic activity. [0454] In certain embodiments, the present disclosure provides methods of reducing cellular metabolic activity in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0455] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by reducing cellular metabolic activity in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0456] In certain embodiments, the present disclosure provides methods of reducing cell proliferation in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0457] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by reducing cell proliferation in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0458] In certain embodiments, the present disclosure provides methods of reducing inflammatory cell infiltration in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0459] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by reducing inflammatory cell infiltration in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0460] In certain embodiments, the present disclosure provides methods of modulating production of nicotinamide adenine dinucleotide in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, cocrystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting the production of nicotinamide adenine dinucleotide. In some embodiments, the modulating is increasing the production of nicotinamide adenine dinucleotide. [0461] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating the production of nicotinamide adenine dinucleotide in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting the production of nicotinamide adenine dinucleotide. In some embodiments, the modulating is increasing the production of nicotinamide adenine dinucleotide.
[0462] In certain embodiments, the present disclosure provides methods of inhibiting production of nicotinamide adenine dinucleotide in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, cocrystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0463] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by inhibiting the production of nicotinamide adenine dinucleotide in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0464] In certain embodiments, the present disclosure provides methods of modulating production of nicotinamide mononucleotide in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, cocrystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting the production of nicotinamide adenine dinucleotide. In some embodiments, the modulating is increasing the production of nicotinamide adenine dinucleotide. [0465] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating the production of nicotinamide mononucleotide in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting the production of nicotinamide adenine dinucleotide. In some embodiments, the modulating is increasing the production of nicotinamide adenine dinucleotide.
[0466] In certain embodiments, the present disclosure provides methods of inhibiting production of nicotinamide mononucleotide in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, cocrystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0467] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by inhibiting the production of nicotinamide mononucleotide in the subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0468] In certain embodiments, the present disclosure provides methods of modulating a NAMPT pathway in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting the NAMPT pathway. In some embodiments, the modulating is activating the NAMPT pathway.
[0469] In certain embodiments, the present disclosure provides methods of inhibiting a NAMPT pathway in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0470] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating a NAMPT pathway in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting a NAMPT pathway. In some embodiments, the modulating is activating a NAMPT pathway.
[0471] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by inhibiting a NAMPT pathway in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof.
[0472] In certain embodiments, the present disclosure provides methods of modulating NAMPT signaling in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting NAMPT signaling. In some embodiments, the modulating is activating signaling. [0473] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by modulating NAMPT signaling in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the modulating is inhibiting NAMPT signaling. In some embodiments, the modulating is activating NAMPT signaling. [0474] In certain embodiments, the present disclosure provides methods of decreasing NAMPT signaling in a subject, the method comprising administering to the subject a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0475] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject by decreasing NAMPT signaling in the subject, the method comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0476] In certain embodiments, the present disclosure provides methods of treating a disease or disorder in a subject comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co- crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the disease or disorder is associated with changes in NAMPT, NMN, NAD, cellular metabolism, and/or NAMPT signaling. In some embodiments, the disease or disorder is mediated by NAMPT, NMN, NAD, cellular metabolism, and/or NAMPT signaling. [0477] In some embodiments, the disclosure provides methods and uses for treating or preventing a disease or disorder described herein in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating or preventing a disease or disorder described herein in a subject in need thereof comprising administering to the subject a therapeutically effective amount of composition comprising a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating or preventing a disease or disorder described herein in a subject in need thereof comprising administering to the subject a therapeutically effective amount of compound as described in the Additional Compounds section, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating or preventing a disease or disorder described herein in a subject in need thereof comprising administering to the subject a therapeutically effective amount of composition comprising a compound as described in the Additional Compounds section, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating or preventing a disease or disorder described herein in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound of Table E1 or Table E2, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating or preventing a disease or disorder described herein in a subject in need thereof comprising administering to the subject a therapeutically effective amount of composition comprising a compound of Table E1 or Table E2, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disease or disorder is associated with changes in NAMPT, NMN, NAD, cellular metabolism, and/or NAMPT signaling. In some embodiments, the disease or disorder is mediated by NAMPT, NMN, NAD, cellular metabolism, and/or NAMPT signaling. [0478] In some embodiments, the disease or disorder is associated with changes in levels or concentrations of NAMPT, NMN, or NAD, cellular metabolism, and/or NAMPT signaling, wherein the disease or disorder is cancer, heart failure, dilated cardiomyopathy, pain, inflammation, acute respiratory distress syndrome, ventilator-induced lung injury, graft-versus-host disease (GCDH), arthritis, rheumatoid arthritis, acute lung injury, pneumonia, pneumonitis, severe acute respiratory distress syndrome, colitis, inflammatory bowel disease (IBD), diabetes, obesity, axon degeneration, a tissue repair disorder, asthma, chronic obstructive pulmonary disease (COPD), osteoarthritis, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra-violet induced skin damage, an autoimmune disease, Alzheimer’s disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, inflammation associated with infection, vascular inflammation, an atherothrombotic disease, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, atherogenic inflammatory disease, a cardiovascular disorder, a cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, an intrauterine infection, Crohn’s disease (CD), ulcerative colitis (UC), a neurodegenerative disorder, viral infections, COVID-19, pulmonary inflammation, a coronavirus infection, and a tumor. [0479] In some embodiments, the disease or disorder is associated with changes in levels or concentrations of NAMPT, NMN, or NAD, cellular metabolism, and/or NAMPT signaling, wherein the disease or disorder is cancer, heart failure, dilated cardiomyopathy, pain, inflammation, acute respiratory distress syndrome, ventilator-induced lung injury, graft-versus-host disease (GCDH), arthritis, rheumatoid arthritis, acute lung injury, pneumonia, pneumonitis, severe acute respiratory distress syndrome, colitis, inflammatory bowel disease (IBD), diabetes, obesity, weight loss, aging, axon degeneration, a tissue repair disorder, asthma, chronic obstructive pulmonary disease (COPD), osteoarthritis, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra-violet induced skin damage, an autoimmune disease, Alzheimer’s disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, inflammation associated with infection, vascular inflammation, an atherothrombotic disease, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, atherogenic inflammatory disease, a cardiovascular disorder, a cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, an intrauterine infection, Crohn’s disease (CD), ulcerative colitis (UC), a neurodegenerative disorder, COVID-19, pulmonary inflammation, a coronavirus infection, and a tumor. [0480] In some embodiments, the disease or disorder is mediated by NAMPT, NMN, NAD, cellular metabolism, and/or NAMPT signaling, wherein the disease or disorder is cancer, heart failure, dilated cardiomyopathy, pain, inflammation, acute respiratory distress syndrome, ventilator-induced lung injury, graft-versus-host disease (GCDH), arthritis, rheumatoid arthritis, acute lung injury, pneumonia, pneumonitis, severe acute respiratory distress syndrome, colitis, inflammatory bowel disease (IBD), diabetes, obesity, axon degeneration, a tissue repair disorder, asthma, chronic obstructive pulmonary disease (COPD), osteoarthritis, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra-violet induced skin damage, an autoimmune disease, Alzheimer's disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, inflammation associated with infection, vascular inflammation, an atherothrombotic disease, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, atherogenic inflammatory disease, a cardiovascular disorder, a cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, an intrauterine infection, Crohn’s disease (CD), ulcerative colitis (UC), a neurodegenerative disorder, COVID-19, pulmonary inflammation, a coronavirus infection, and a tumor. [0481] In some embodiments, the disease or disorder is mediated by NAMPT, NMN, NAD, cellular metabolism, and/or NAMPT signaling, wherein the disease or disorder is cancer, heart failure, dilated cardiomyopathy, pain, inflammation, acute respiratory distress syndrome, ventilator-induced lung injury, graft-versus-host disease (GCDH), arthritis, rheumatoid arthritis, acute lung injury, pneumonia, pneumonitis, severe acute respiratory distress syndrome, colitis, inflammatory bowel disease (IBD), diabetes, obesity, weight loss, aging, axon degeneration, a tissue repair disorder, asthma, chronic obstructive pulmonary disease (COPD), osteoarthritis, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra-violet induced skin damage, an autoimmune disease, Alzheimer's disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, inflammation associated with infection, vascular inflammation, an atherothrombotic disease, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, atherogenic inflammatory disease, a cardiovascular disorder, a cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, an intrauterine infection, Crohn’s disease (CD), ulcerative colitis (UC), a neurodegenerative disorder, COVID-19, pulmonary inflammation, a coronavirus infection, and a tumor.
[0482] In some embodiments, the disease or disorder is ophthalmic disease or disorder. In certain embodiments, the ophthalmic disease or disorder is selected from dry eye, retinal disease, glaucoma, retinal degeneration, macular degeneration, diabetic retinopathy, retinitis pigmentosa, retinal vein occlusion, retinal vascular occlusion, traumatic optic neuropathy, neurotrophic keratopathy, Leber congenital amaurosis, diabetic macular edema (DME), proliferative diabetic retinopathy (PDR), and optic nerve degeneration.
[0483] In some embodiments, the disease or disorder is cancer, heart failure, dilated cardiomyopathy, pain, inflammation, acute respiratory distress syndrome, ventilator-induced lung injury, graft- versus- host disease (GCDH), arthritis, rheumatoid arthritis, acute lung injury, pneumonia, pneumonitis, severe acute respiratory distress syndrome, colitis, inflammatory bowel disease (IBD), diabetes, obesity, axon degeneration, a tissue repair disorder, asthma, chronic obstructive pulmonary disease (COPD), osteoarthritis, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultraviolet induced skin damage, an autoimmune disease, Alzheimer's disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, inflammation associated with infection, vascular inflammation, an atherothrombotic disease, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, atherogenic inflammatory disease, a cardiovascular disorder, a cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, an intrauterine infection, Crohn’s disease (CD), ulcerative colitis (UC), a neurodegenerative disorder, COVID-19, pulmonary inflammation, a coronavirus infection, and a tumor.
[0484] In some embodiments, the disease or disorder is cancer, heart failure, dilated cardiomyopathy, pain, inflammation, acute respiratory distress syndrome, ventilator-induced lung injury, graft- versus- host disease (GCDH), arthritis, rheumatoid arthritis, acute lung injury, pneumonia, pneumonitis, severe acute respiratory distress syndrome, colitis, inflammatory bowel disease (IBD), diabetes, obesity, weight loss, aging, axon degeneration, a tissue repair disorder, asthma, chronic obstructive pulmonary disease (COPD), osteoarthritis, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra-violet induced skin damage, an autoimmune disease, Alzheimer’s disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, inflammation associated with infection, vascular inflammation, an atherothrombotic disease, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, atherogenic inflammatory disease, a cardiovascular disorder, a cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, an intrauterine infection, Crohn’s disease (CD), ulcerative colitis (UC), a neurodegenerative disorder, COVID-19, pulmonary inflammation, a coronavirus infection, and a tumor. [0485] In some embodiments, the disease or disorder is selected from heart failure, dilated cardiomyopathy, pain, graft-versus-host disease (GCDH), diabetes, obesity, axon degeneration, tissue repair disorders, asthma, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra- violet induced skin damage, Alzheimer’s disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, cardiovascular disorder, cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, intrauterine infection, neurodegenerative disorder, and a tumor. [0486] In some embodiments, the disease or disorder is selected from heart failure, dilated cardiomyopathy, pain, graft-versus-host disease (GCDH), diabetes, obesity, weight loss, aging, axon degeneration, tissue repair disorders, asthma, osteoporosis, fibrotic diseases, dermatosis, psoriasis, atopic dermatitis, ultra-violet induced skin damage, Alzheimer’s disease, stroke, atherosclerosis, restenosis, glomerulonephritis, cachexia, Acquired Immune Deficiency Syndrome (AIDS), adult respiratory distress syndrome, ataxia telangiectasia, cardiovascular disorder, cerebrovascular disorder, acute coronary syndrome, polycystic ovary syndrome, preeclampsia, sepsis, septic shock, intrauterine infection, neurodegenerative disorder, and a tumor. [0487] In some embodiments, the disease or disorder is selected from diabetes, rheumatoid arthritis, inflammatory bowel disease, acute respiratory distress syndrome, and ventilator-induced lung injury. [0488] In some embodiments, the disease or disorder is cancer. In some embodiments, the disease or disorder is heart failure. In some embodiments, the disease or disorder is dilated cardiomyopathy. In some embodiments, the disease or disorder is pain. In certain embodiments, the disease or disorder is acute respiratory distress syndrome. In some embodiments, the disease or disorder is ventilator- induced lung injury. In some embodiments, the disease or disorder is inflammation. In some embodiments, the disease or disorder is graft-versus-host disease (GCDH). In some embodiments, the disease or disorder is arthritis. In some embodiments, the disease or disorder is acute lung injury. In some embodiments, the disease or disorder is colitis. In some embodiments, the disease or disorder is inflammatory bowel disease (IBD). In some embodiments, the disease or disorder is diabetes. In some embodiments, the disease or disorder is obesity. In some embodiments, the disease or disorder is weight loss. In some embodiments, the disease or disorder is aging. In some embodiments, the disease or disorder is axon degeneration. In some embodiments, the disease or disorder is a tissue repair disorder. In some embodiments, the disease or disorder is asthma. In some embodiments, the disease or disorder is chronic obstructive pulmonary disease (COPD). In some embodiments, the disease or disorder is osteoarthritis. In some embodiments, the disease or disorder is osteoporosis. In some embodiments, the disease or disorder is fibrotic diseases. In some embodiments, the disease or disorder is dermatosis. In some embodiments, the disease or disorder is psoriasis. In some embodiments, the disease or disorder is atopic dermatitis. In some embodiments, the disease or disorder is ultra-violet induced skin damage. In some embodiments, the disease or disorder is an autoimmune disease. In some embodiments, the disease or disorder is Alzheimer’s disease. In some embodiments, the disease or disorder is stroke. In some embodiments, the disease or disorder is atherosclerosis. In some embodiments, the disease or disorder is restenosis. In some embodiments, the disease or disorder is glomerulonephritis. In some embodiments, the disease or disorder is cachexia.
In some embodiments, the disease or disorder is inflammation associated with infection. In some embodiments, the disease or disorder is vascular inflammation. In some embodiments, the disease or disorder is an atherothrombotic disease. In some embodiments, the disease or disorder is Acquired Immune Deficiency Syndrome (AIDS). In some embodiments, the disease or disorder is adult respiratory distress syndrome. In some embodiments, the disease or disorder is ataxia telangiectasia.
In some embodiments, the disease or disorder is atherogenic inflammatory disease. In some embodiments, the disease or disorder is a cardiovascular disorder. In some embodiments, the disease or disorder is a cerebrovascular disorder. In some embodiments, the disease or disorder is acute coronary syndrome. In some embodiments, the disease or disorder is polycystic ovary syndrome. In some embodiments, the disease or disorder is preeclampsia. In some embodiments, the disease or disorder is sepsis. In some embodiments, the disease or disorder is septic shock. In some embodiments, the disease or disorder is an intrauterine infection. In some embodiments, the disease or disorder is Crohn’s disease (CD). In some embodiments, the disease or disorder is ulcerative colitis (UC). In some embodiments, the disease or disorder is a neurodegenerative disorder. In some embodiments, the disease or disorder is a tumor. In some embodiments, the disease or disorder is COVID-19. In some embodiments, the disease or disorder is pulmonary inflammation. In some embodiments, the disease or disorder is a coronavirus infection. In certain embodiments, the disease or disorder is pneumonia. In some embodiments, the disease or disorder is pneumonitis. In some embodiments, the disease or disorder is severe acute respiratory distress syndrome.
[0489] In some embodiments, the autoimmune disease is systemic lupus erythematosus, multiple sclerosis, ankylosing spondylitis, tissue and organ rejection, glomerulonephritis, Goodpasture’s syndrome, necrotizing vasculitis, lymphadenitis, peri-arteritis nodosa, rheumatoid arthritis, psoriatic arthritis, psoriasis, ulcerative colitis, systemic sclerosis, dermatomyositis/polymyositis, antiphospholipid antibody syndrome, scleroderma, pemphigus vulgaris, ANCA-associated vasculitis (e.g., Wegener’s granulomatosis, microscopic poly angiitis), uveitis, Sjogren’s syndrome, Crohn’s disease, Reiter’s syndrome, ankylosing spondylitis, Lyme disease, Guillain-Barre syndrome, Hashimoto’s thyroiditis, and cardiomyopathy. In some embodiments, the autoimmune disease is systemic lupus erythematosus, multiple sclerosis, ankylosing spondylitis, or tissue and organ rejection. [0490] In some embodiments, the graft-versus host disease is acute graft-versus-host disease or chronic graft-versus-host disease. [0491] In some embodiments, the arthritis is osteoarthritis, rheumatoid arthritis, psoriatic arthritis, juvenile arthritis, fibromyalgia, gout, lupus, ankylosing spondylitis, reactive arthritis, septic arthritis, thumb arthritis, knee arthritis, infectious arthritis, degenerative arthritis, Reiter’s arthritis, crystalline arthritis or inflammatory arthritis. In some embodiments, the arthritis is osteoarthritis, rheumatoid arthritis, or psoriatic arthritis. In some embodiments, the arthritis is osteoarthritis or rheumatoid arthritis. [0492] In some embodiments, the neurodegenerative disorder is Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, tauopathies (including frontotemporal dementia), Huntington’s disease, multiple sclerosis, Friedreich’s ataxia, Lewy body disease, or spinal muscular atrophy. [0493] In some embodiments, the intrauterine infection is chorioamnionitis. [0494] In some embodiments, the diabetes is type 2 diabetes. [0495] In some embodiments, the cerebrovascular disorder is stroke, carotid stenosis, vertebral stenosis and intracranial stenosis, aneurysms, or vascular malformations. In some embodiments, the cerebrovascular disorder is stroke. [0496] In some embodiments, the cardiovascular disorder is acute coronary syndrome, heart failure, dilated cardiomyopathy, or cardiomyopathy. [0497] In certain embodiments, the tumor is a solid tumor. In some embodiments, the tumor is a colorectal tumor, ovarian tumor, breast tumor, gastric tumor, prostate tumor, thyroid tumor, pancreatic tumor, melanoma, gliomas, sarcoma, endometrial tumor, carcinoma tumor, or hematological malignancy. [0498] Some aspects of the invention relate to methods, uses, compositions, and kits for administration to a subject in need thereof. In some embodiments, the subject is a subject having, suspected of having, or at risk of developing a disease or disorder (e.g., proliferative disease, cancer). As used herein, “subject,” “individual,” and “patient” may be used interchangeably. In some embodiments, the subject is a mammalian subject, including but not limited to a dog, cat, horse, cow, pig, sheep, goat, chicken, rodent, or primate. In some embodiments, the subject is a human subject, such as a patient. The human subject may be a pediatric or adult subject. [0499] As used herein “treating” includes amelioration, cure, prevent it from becoming worse, slow the rate of progression, to prevent the disorder from re-occurring (i.e., to prevent a relapse), or to prevent or slow the rate of metastasis. An effective amount of a compound or composition refers to an amount of the compound or composition that results in a therapeutic effect. For example, in methods or uses for treating cancer in a subject, an effective amount of a chemotherapeutic agent is any amount that provides an anti-cancer effect, such as reduces or prevents proliferation of a cancer cell or is cytotoxic towards a cancer cell. [0500] The methods and uses disclosed herein involve administering any of the compounds of the disclosure or compositions described herein in an effective amount to a subject. In certain embodiments the subject has a proliferative disease. In other embodiments, the subject is in need of regenerative medicine. In certain aspects, the uses and methods inhibit tumor growth, regenerate or differentiate one or more cells, prevent metastasis, kill cancer cells, reduce embryonic properties or adult stem cell properties of one or more cells, reduce cell viability, and/or prevent cell proliferation. [0501] The methods and uses disclosed herein involve administering any of the compounds of the disclosure or compositions described herein in an effective amount to a subject having a proliferative disease. In some embodiments, the proliferative disease is cancer. In some embodiments, the proliferative disease is a benign neoplasm. [0502] The methods and uses disclosed herein involve administering any of the compounds of the disclosure or compositions described herein in an effective amount to a subject in need of regenerative medicine or regenerative therapy. In some embodiments, the subject is in need of restoring or improving one or more biological function of a cell, tissue, and/or organ that is dysfunctional or impaired. In some embodiments, the subject is in need of tissue engineering and organ regeneration. In some embodiments, the compounds or compositions described herein regenerate or differentiate cells, tissues, and/or organs that may be damaged. [0503] Methods and uses disclosed herein involve administering any of the compounds of the disclosure or compositions described herein in an effective amount to a subject having cancer or at risk of having cancer. In some embodiments, the cancer is characterized by the presence of cancer stem cells. In some embodiments, the cancer comprises, involves, or is associated with stem cells. In some embodiments, the subject has undergone or is currently undergoing a cancer therapy (e.g. chemotherapeutic, immunotherapeutic, surgery, radiation). Whether a subject is deemed “at risk” of having a disease or disorder, such as cancer, may be determined by a skilled practitioner. [0504] In some embodiments, the disclosure provides methods and uses for treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of composition comprising a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of compound as described in the Additional Compounds section, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of composition comprising a compound as described in the Additional Compounds section, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound of Table E1 or Table E2, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In some embodiments, the disclosure provides methods and uses for treating cancer in a subject in need thereof comprising administering to the subject a therapeutically effective amount of composition comprising a compound of Table E1 or Table E2, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. [0505] In certain embodiments, the cancer is colorectal cancer. Colorectal cancer is a cancer that starts in the colon or the rectum. These cancers may also be referred to as colon cancer or rectal cancer, depending on where the cancer begins. Colon cancer and rectal cancer are often grouped together due to several shared features. Most colorectal cancers start as a growth on the inner lining of the colon or rectum. The colorectal cancer (CRC) Subtyping Consortium has unified six independent molecular classification systems, based on gene expression data, into a single consensus system with four distinct groups, known as the Consensus Molecular Subtypes (CMS). The CMS were determined and correlated with epigenomic, transcriptomic, microenvironmental, genetic, prognostic and clinical characteristics. The CMS1 subtype is immunogenic and hypermutated. CMS2 tumors are activated by the WNT-β-catenin pathway and generally are associated with higher overall survival rates. CMS3 feature a metabolic cancer phenotype. CMS4 cancers are associated with the lowest survival rates and have a strong stromal gene signature. Molecular subtypes CMS2 and CMS4 exhibit the highest levels of embryonic signaling. 11 [0506] In certain embodiments, the cancer is gastric cancer. Gastric cancer is a cancer that begins in the stomach. Stomach cancers tend to develop slowly over many years. Before a true cancer develops, pre-cancerous changes often occur in the inner lining (mucosa) of the stomach. These early changes rarely cause symptoms and therefore often go undetected. The types of stomach cancer include adenocarcinoma, lymphoma, gastrointestinal stromal tumor (GIST), carcinoid tumor, squamous cell carcinoma, small cell carcinoma, and leiomyosarcoma. The Cancer Genome Atlas (TCGA) project recently uncovered four molecular subtypes of gastric cancer: Epstein-Barr virus (EBV), microsatellite instability (MSI), genomically stable (GS), and chromosomal instability (CIN). The GS (genomically stable) and CIN (chromosomal instability) molecular subtypes exhibit the highest levels embryonic signaling, as measured by comprehensive analysis of gene expression patterns across gastric cancer subtypes. 12,13 [0507] In some embodiments, the cancer is ovarian cancer. In certain embodiments, the cancer is lung cancer. In some embodiments, the cancer is breast cancer. In some embodiments, the cancer is pancreatic cancer. In certain embodiments, the cancer is prostate cancer. In some embodiments, the cancer is testicular cancer. In certain embodiments, the cancer is liver cancer. In some embodiments, the cancer is endometrial cancer. [0508] In some embodiments, the cancer is lymphoma. In certain embodiments, the cancer is non- Hodgkin’s lymphoma. In some embodiments, the cancer is Hodgkin’s lymphoma. In some embodiments, the cancer is B-cell lymphoma (e.g., large B-cell lymphoma). In certain embodiments, the cancer is Burkitt’s lymphoma (e.g., Burkitt’s B-cell lymphoma). In some embodiments, the cancer is large cell immunoblastic lymphoma. [0509] In certain embodiments, the cancer is leukemia. In certain embodiments, the cancer is acute lymphocytic leukemia (ALL), acute myelogenous leukemia (AML) (also known as acute myeloid leukemia, acute myeloblastic leukemia, acute granulocytic leukemia, acute myelocytic leukemia, and acute nonlymphocytic leukemia), chronic lymphocytic leukemia (CLL), or chronic myelogenous leukemia (CML) (also known as chronic myeloid leukemia). In certain embodiments, the cancer is AML. In some embodiments, the cancer is a subtype of AML selected from undifferentiated acute myeloblastic leukemia (M0), acute myeloblastic leukemia with minimal maturation (M1), acute myeloblastic leukemia with maturation (M2), acute promyelocytic leukemia (APL) (M3), acute myelomonocytic leukemia (M4), acute myelomonocytic leukemia with eosinophilia (M4 eos), acute monocytic leukemia (M5), acute erythroid leukemia (M6), and acute megakaryoblastic leukemia (M7). In some embodiments, the cancer is acute monocytic leukemia or acute lymphocytic leukemia (e.g., B-cell acute lymphocytic leukemia). In some embodiments, the cancer is acute lymphoblastic leukemia (e.g., B-cell acute lymphoblastic leukemia or T-cell acute lymphoblastic leukemia). [0510] In some embodiments, the cancer is myeloma. In certain embodiments, plasmacytoma. In certain embodiments, the cancer is localized myeloma. In some embodiments, the cancer is extramedullary myeloma. In some embodiments, the cancer is multiple myeloma. In certain embodiments, the cancer is B-cell myeloma. [0511] In some embodiments, the cancer is small cell lung cancer. In some embodiments, the cancer is small cell carcinoma (oat cell cancer). In some embodiments, the cancer is combined small cell carcinoma. [0512] In some embodiments, the cancer is breast cancer. In some embodiments, the cancer is ductal carcinoma in situ (DCIS). In some embodiments, the cancer is invasive ductal carcinoma (IDC). In some embodiments, the cancer is tubular carcinoma of the breast. In some embodiments, the cancer is medullary carcinoma of the breast. In some embodiments, the cancer is mucinous Carcinoma of the breast. In some embodiments, the cancer is papillary carcinoma of the breast. In some embodiments, the cancer is cribriform carcinoma of the breast. In some embodiments, the cancer is invasive lobular carcinoma (ILC). In some embodiments, the cancer is inflammatory breast cancer. In some embodiments, the cancer is lobular carcinoma in situ (LCIS). In some embodiments, the cancer is male breast cancer. In some embodiments, the cancer is molecular subtypes of breast cancer. In some embodiments, the cancer is Paget’s disease of the nipple. In some embodiments, the cancer is phyllodes tumors of the breast. In some embodiments, the cancer is metastatic breast cancer. [0513] In certain embodiments, the cancer is colorectal cancer, gastric cancer, gastrointestinal stromal tumor, ovarian cancer, lung cancer, breast cancer, pancreatic cancer, testicular cancer, prostate cancer, liver cancer, or endometrial cancer. In certain embodiments, the cancer is leukemia (e.g., acute myeloid leukemia). In certain embodiments, the cancer is lymphoma. In certain embodiments, the cancer is multiple myeloma. In certain embodiments, the subject is in need of regenerative medicine or therapy. In some embodiments, the cancer is selected from breast, prostate, lung, colon, cervix, ovary, skin, CNS, bladder, pancreas, leukemia, lymphoma, and Hodgkin’s disease. [0514] In certain embodiments, the subject has been administered an additional therapy. In certain embodiments, the subject is further administered (co-administration) an additional therapy (e.g., before, concurrently with, and/or after the administration of a compound or composition described herein). The additional therapy is different from a compound or composition described herein. In certain embodiments, the additional therapy alone is ineffective, or less effective as compared with co-administration with (e.g., before, concurrently with, and/or after) a compound or composition described herein, in a method or use described herein. [0515] In some embodiments, the additional therapy is radiation. In some embodiments, the radiation is fractionate radiation. In some embodiments, the additional therapy is surgery. In some embodiments, the additional therapy is an organ transplant. In some embodiments, the additional therapy is a kidney transplant. In some embodiments, the additional therapy is a bone marrow transplant. In certain embodiments, the additional therapy is a lung transplant. In some embodiments, the additional therapy is T cell replacement therapy. [0516] In certain embodiments, the disclosure provides methods of treating a disease or disorder in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co- crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein, and an additional pharmaceutical agent. In some embodiments, the additional pharmaceutical agent is administered, before, concurrently with, or after the compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0517] In certain embodiments, the subject has been administered an additional pharmaceutical agent. In certain embodiments, the subject is further administered (co-administration) an additional pharmaceutical agent (e.g., before, concurrently with, and/or after the administration of a compound or composition described herein). The additional pharmaceutical agent is different from a compound or composition described herein. In certain embodiments, the additional therapy alone is ineffective, or less effective as compared with co-administration with (e.g., before, concurrently with, and/or after) a compound or composition described herein, in a method or use described herein. [0518] The exact amount of a compound of the disclosure required to achieve an effective amount will vary from subject to subject, depending, for example, on species, age, and general condition of a subject, severity of the side effects or disorder, identity of the particular compound of the disclosure, mode of administration, and the like. [0519] An effective amount may be included in a single dose (e.g., single oral dose) or multiple doses (e.g., multiple oral doses). In certain embodiments, when multiple doses are administered to a subject or applied to a biological sample, tissue, or cell, any two doses of the multiple doses include different or substantially the same amounts of an agent described herein. [0520] In certain embodiments, when multiple doses are administered to a subject or applied to a tissue or cell, the frequency of administering the multiple doses to the subject or applying the multiple doses to the tissue or cell is three doses a day, two doses a day, one dose a day, one dose every other day, one dose every third day, one dose every week, one dose every two weeks, one dose every three weeks, or one dose every four weeks or longer. In certain embodiments, when multiple doses are administered to a subject or applied to a biological sample, tissue, or cell, the duration between the first dose and last dose of the multiple doses is one day, two days, four days, one week, two weeks, three weeks, one month, two months, three months, four months, six months, nine months, one year, two years, three years, four years, five years, seven years, ten years, fifteen years, twenty years, or the lifetime of the subject, tissue, or cell. In certain embodiments, the duration between the first dose and last dose of the multiple doses is three months, six months, or one year. In certain embodiments, the duration between the first dose and last dose of the multiple doses is the lifetime of the subject, tissue, or cell. [0521] Any of the compounds or compositions described herein may be administered in a therapeutically effective amount. In some embodiments, the methods and uses involve administering a compound or composition comprising any of the compounds described herein to achieve a desired amount (e.g., a therapeutically effective amount) of the compound at a particular site in the subject. In some embodiments, the methods and uses involve administering a compound or composition comprising any of the compounds described herein to achieve a desired amount (e.g., a therapeutically effective amount) of the compound at the site of a tumor in the subject. [0522] Dosage may be adjusted appropriately to achieve a desired local level of the compound. [0523] “Dose” and “dosage” are used interchangeably herein. In some embodiments, the amount of the compound administered to a subject is about 0.1 µg and 1 µg, between 0.001 mg and 0.01 mg, between 0.01 mg and 0.1 mg, between 0.1 mg and 1 mg, between 1 mg and 3 mg, between 3 mg and 10 mg, between 10 mg and 30 mg, between 30 mg and 100 mg, between 100 mg and 300 mg, between 300 mg and 1,000 mg, or between 1 g and 10 g, inclusive, of a compound of the disclosure described herein. In certain embodiments, a dose described herein includes independently between 1 mg and 3 mg, inclusive, of a compound of the disclosure described herein. In certain embodiments, a dose described herein includes independently between 3 mg and 10 mg, inclusive, of a compound of the disclosure described herein. In certain embodiments, a dose described herein includes independently between 10 mg and 30 mg, inclusive, of a compound of the disclosure described herein. In certain embodiments, a dose described herein includes independently between 30 mg and 100 mg, inclusive, of a compound of the disclosure described herein. [0524] In some embodiments, the subject is administered an initial dose of any one of the compounds or compositions described herein, followed by one or more additional doses of any of the compounds or compositions described herein. In some embodiments, the initial dose may contain a different amount of any of the compounds described herein as compared to the one or more additional doses. In some embodiments, the initial dose is a higher dose (e.g., contains more of any one of the compounds described herein) as compared to the one or more additional doses. [0525] Dose ranges as described herein provide guidance for the administration of provided compounds or compositions to an adult. The amount to be administered to, for example, a child or an adolescent can be determined by a medical practitioner or person skilled in the art and can be lower or the same as that administered to an adult. In certain embodiments, a dose described herein is a dose to an adult human whose body weight is 70 kg. [0526] Efficacy in treating cancer, for example, can be measured by determining the growth, replication, proliferation, metastasis, and/or gene expression profile of one or more cancer cells. An effective amount, therefore, is an amount that is deemed by the clinician to be toxicologically tolerable, yet efficacious. [0527] Without being bound to a particular theory, the compounds disclosed herein are thought to induce the differentiation of embryonic cells and/or cells exhibiting characteristics of embryonic cells and/or induce the differentiation of adult stem cells and/or cells exhibiting characteristics of adult stem cells. In some embodiments, the methods and uses disclosed herein involve administering any of the compounds or compositions described herein in an effective amount to a subject in need of regenerative medicine or regenerative therapy. In some embodiments, the subject is in need of restoring or improving one or more biological function of a cell, tissue, and/or organ that is dysfunctional or impaired. In some embodiments, the subject is in need of tissue engineering and organ regeneration. In some embodiments, the compounds or compositions described herein regenerate or differentiate cells, tissues, and/or organs that may be damaged. In some embodiments, the subject has experienced brain injury (e.g., injury or damage to the brain tissue or cells) and/or injury to the central nervous system (e.g., injury or damage to the tissue or cells of the central nervous system) and is in need of repair of said tissue or cells. In some embodiments, the subject has experienced heart injury (e.g., injury or damage to the heart tissue or cells) and is in need of repair of said tissue or cells. [0528] In some embodiments, the administration of any of the compounds or compositions described herein is by oral administration, intravenous administration (e.g., systemic intravenous injection), parental administration, subcutaneous administration, intramuscular administration, mucosal administration, transdermal administration, intradermal administration, intravaginal administration, intraperitoneal administration, topical administration, nasal administration, buccal administration, sublingual administration; by intratracheal regional administration via blood and/or lymph supply, and/or direct administration to an affected site. [0529] The present disclosure also provides methods for contacting a cell with an effective amount of a compound of the disclosure (e.g., a compound of Formula (I), (II), (III), or (IV), or subgenera or species thereof, a compound of Tables E1 or E2, or a compound as otherwise provided herein). The present disclosure also provides uses for contacting a cell with an effective amount of a compound of the disclosure. In certain aspects, the uses and methods inhibit tumor growth, regenerate or differentiate one or more cells, prevent metastasis, kill cancer cells, reduce embryonic properties or adult stem cell properties of one or more cells, reduce cell viability, and/or prevent cell proliferation. [0530] In certain aspects, the present disclosure provides methods and uses comprising contacting a compound of Formula (I), (II), (III), or (IV), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, with a cell, tissue, or biological sample to inhibit tumor growth, regenerate or differentiate one or more cells, prevent metastasis, kill cancer cells, reduce embryonic properties or adult stem cell properties of one or more cells, reduce cell viability, and/or prevent cell proliferation. [0531] The present disclosure also provides methods of modulating NAMPT in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co- crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting NAMPT. In some embodiments, the modulating is activating NAMPT. [0532] The present disclosure also provides methods of inhibiting NAMPT in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co- crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0533] The present disclosure also provides methods of modulating production of nicotinamide adenine dinucleotide in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting NAMPT. In some embodiments, the modulating is activating NAMPT. [0534] The present disclosure also provides methods of inhibiting production of nicotinamide adenine dinucleotide in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0535] The present disclosure also provides methods of modulating production of nicotinamide mononucleotide in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting NAMPT. In some embodiments, the modulating is activating NAMPT. [0536] The present disclosure also provides methods of inhibiting production of nicotinamide mononucleotide in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0537] The present disclosure also provides methods of reducing inflammatory cell infiltration in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0538] The present disclosure also provides methods of reducing cell proliferation in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co- crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0539] The present disclosure also provides methods of modulating cellular metabolic activity in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting cellular metabolic activity. In some embodiments, the modulating is activating cellular metabolic activity. [0540] The present disclosure also provides methods of reducing cellular metabolic activity in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein.
[0541] The present disclosure also provides methods of modulating cellular metabolism a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, cocrystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting cellular metabolism. In some embodiments, the modulating is activating cellular metabolism.
[0542] The present disclosure also provides methods of reducing cellular metabolism in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, cocrystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein.
[0543] The present disclosure also provides methods of modulating inflammatory activity in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting inflammatory activity. In some embodiments, the modulating is activating inflammatory activity.
[0544] The present disclosure also provides methods of decreasing inflammatory activity in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein.
[0545] The present disclosure also provides methods of modulating NAMPT signaling in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting NAMPT signaling. In some embodiments, the modulating is activating NAMPT signaling.
[0546] The present disclosure also provides methods of decreasing NAMPT signaling in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein.
[0547] The present disclosure also provides methods of modulating a NAMPT pathway in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. In some embodiments, the modulating is inhibiting a NAMPT pathway. In some embodiments, the modulating is activating a NAMPT pathway. [0548] The present disclosure also provides methods of inhibiting a NAMPT pathway in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or composition disclosed herein. [0549] In some embodiments, any of the compounds described herein are contacted with a cell in vivo, e.g. in an organism. In some embodiments, any of the compounds described herein are contacted with a cell in vitro, e.g., in cell culture. In some embodiments, any of the compounds described herein are contacted with a cell ex vivo, meaning the cell is removed from an organism prior to the contacting. As will be evident to one of skill in the art, the term cell may be used to refer to a single cell as well as a population of cells. In some embodiments, the populations cells are contacted with any of the compounds described herein to regenerate or differentiate one or more cells in the population of cells. In some embodiments, the populations cells are contacted with any of the compounds described herein for use in personalized medicine, for example for diagnostic and/or therapeutic purposes. [0550] In general, any cells known in the art may be used in the methods and uses described herein. In some embodiments, the cell is of a cell line. In some embodiments, the cell is obtained from an organism, such as a subject. In some embodiments, the cell is a cancer cell (e.g., a cancer stem cell). In some embodiments, the cell is a stem cell. In some embodiments, the cell is an adult stem cell. In some embodiments, the cell is an embryonic stem cell. In some embodiments, the cell is an induced pluripotent stem cell. In some embodiments, the cell is a neural cell, such as a neural stem cell. In some embodiments, the cell is an adult stem cell, such as a stomach stem cell or intestinal stem cell. [0551] In some embodiments, the methods and uses further comprise inhibiting the growth of cells. In certain embodiments, the methods and uses comprise contacting a cell with a compound or composition described herein to kill a cell, reduce cell viability, and/or prevent cell proliferation. In some embodiments, the methods and uses comprise contacting a cell with a compound or composition described herein to prevent metastasis or inhibit tumor growth. In certain embodiments, the methods and uses comprise contacting a cell with a compound or composition described herein to regenerate or differentiate one or more cells. In certain embodiments, the methods and uses comprise contacting a cell with a compound or composition described herein to reduce embryonic properties or adult stem cell properties of one or more cells. In other embodiments, the methods and uses comprise killing cells. In some embodiments, the methods and uses comprise reducing cell viability. In some embodiments, the methods and uses comprise preventing cell proliferation. In some embodiments, the cells are stem cells. In some embodiments, the cells are selected from the group consisting of a cancer stem cell, an embryonic stem cell, an induced pluripotent stem cell, a neural stem cell, a differentiated cancer cell, or an adult stem cell. In certain embodiments, the cells are cancer stem cells. In certain embodiments, the cells are embryonic stem cells. In certain embodiments, the cells are adult stem cells. In certain embodiments, the disclosure provides methods and uses of inhibiting the growth of cells and/or killing cells with an effective amount of a compound of the disclosure. In certain aspects, inhibiting the growth of cells and/or killing cells is useful in the treatment of proliferative diseases, including cancer.
[0552] In some embodiments, the methods and uses further comprise measuring or assessing the level of one or more embryonic properties of the cell. In some embodiments, the level of one or more embryonic properties of the cell is assessed following contacting the cell with any of the compounds or compositions described herein. In some embodiments, the level of one or more embryonic properties following contacting the cell with any of the compounds or compositions described herein is compared to the level of one or more embryonic properties in a reference sample or prior to contacting the cell with the compound or composition. In some embodiments, the contacting the cell with any of the compounds or compositions described herein reduces one or more embryonic properties of the cell. In some aspects, the methods and uses described herein may be used to determine whether a cell is susceptible to treatment with the compounds or compositions described herein. In some embodiments, if the level of one or more embryonic properties is reduced following contacting the cell with any of the compounds or compositions described herein, the cell is determined to be susceptible to treatment with the compound or composition. In some embodiments, if the level of one or more embryonic properties is reduced following contacting the cell with any of the compounds or compositions described herein, the compound or composition is determined to be a candidate for a disease or disorder associated with the cell.
[0553] In some embodiments, the methods and uses further comprise measuring or assessing the level of one or more adult stem cell properties of the cell. In some embodiments, the level of one or more adult stem cell properties of the cell is assessed following contacting the cell with any of the compounds or compositions described herein. In some embodiments, the level of one or more adult stem cell properties following contacting the cell with any of the compounds or compositions described herein is compared to the level of one or more adult stem cell properties in a reference sample or prior to contacting the cell with the compounds or composition. In some embodiments, the contacting the cell with any of the compounds or compositions described herein reduces one or more adult stem cell properties of the cell. In some aspects, the methods and uses described herein may be used to determine whether a cell is susceptible to treatment with the compounds or compositions described herein. In some embodiments, if the level of one or more adult stem cell properties is reduced following contacting the cell with any of the compounds or compositions described herein, the cell is determined to be susceptible to treatment with the compound or composition. In some embodiments, if the level of one or more adult stem cell properties is reduced following contacting the cell with any of the compounds or compositions described herein, the compound or composition is determined to be a candidate for a disease or disorder associated with the cell. [0554] In some embodiments, the methods and uses described herein may be used for regenerative medicine. In some embodiments, a cell is contacted with any of the compounds or compositions described herein to promote differentiation and/or loss of one or more embryonic properties of the cell. In some embodiments, a cell is contacted with any of the compounds or compositions described herein to promote differentiation and/or loss of one or more adult stem cell properties of the cell. In some embodiments, a cell is contacted with any of the compounds or compositions described herein to promote regenerative capacity of the cell. In some embodiments, contacting the cell with any of the compounds or compositions described herein enhances the regenerative capacity of the cell. In some embodiments, contacting the cell with any of the compounds or compositions described herein regenerates a population of cells, such as a tissue or an organ. In some embodiments, the regenerated population of cells, such as a tissue or an organ may be administered or implanted into a subject. In some embodiments, the subject is the same subject from which a cell was obtained. In some embodiments, the subject is a different subject from which a cell was obtained (e.g., autotransplantation). In some embodiments, the subject is a different subject from which a cell was obtained but belongs to the same species (e.g., allotransplantation). In some embodiments, the subject is a different subject from which a cell was obtained and belongs to a different species (e.g., xenotransplantation). Methods of Synthesizing [0555] The present disclosure further provides methods of synthesizing the compounds provided herein (e.g., comprising a compound of the disclosure (e.g., a compound of Formula (I) or (II)), or a compound as disclosed in PCT Publication No. WO 2019/213570 or PCT Application No. PCT/US2020/059329, and other compounds of Formula (V). In some embodiments, a compound of Formula (V) is synthesized via Route A. In certain embodiments, a compound of Formula (V) is synthesized via Route B. In certain embodiments, a compound of Formula (V) is synthesized via Route B and Route C. In certain embodiments, a compound of Formula (V) is synthesized via Route D. In certain embodiments, a compound of Formula (V) is synthesized via Route E. [0556] The present disclosure also provides methods of synthesizing compounds of Formula (V-a1), (V-a2), (V-a3), (V-a4), (V-b1), (V-b2), (V-b3), (V-b3i), (V-b3ii), (V-d1), (V-d2), (V-d4), (V-d5), (V-e2), (V-e3), (V-e4), and (V-e5). [0557] In some embodiments, the methods of synthesizing as provided herein (e.g., Routes A, B, C, D, and/or E) are utilized to synthesize compounds disclosed in PCT Publication No. WO 2019/213570 and PCT Application No. PCT/US2020/059329, both of which are incorporated herein by reference in their entirety. In some embodiments, the methods of synthesizing as provided herein (e.g., Routes A, B, C, D, and/or E) are utilized to synthesize compounds disclosed in PCT Application No. PCT/US2020/059329. In some embodiments, the methods of synthesizing as provided herein (e.g., Routes A, B, C, D, and/or E) are advantageous (e.g., higher yield, shorter reaction times, less steps) compared to synthetic procedures as provided in PCT Publication No. WO 2019/213570 and PCT Application No. PCT/US2020/059329. Route A [0558] In some embodiments, a compound of Formula (V) is synthesized via Route A, wherein: X is halo; R* is hydrogen or C 1-4 alkyl, or wherein two instances of R* are joined to form a 3- to 13- membered carbocyclyl or 6- to 12-membered aryl ring; m is an integer selected from 0 or 1; R 2S is substituted or unsubstituted C 1-12 alkyl, substituted or unsubstituted C 1-12 heteroalkyl, substituted or unsubstituted 3-to 13-membered heterocyclyl, or substituted or unsubstituted 3- to 13- membered carbocyclyl, substituted or unsubstituted 3-to 13-membered heterocyclyl-C 1-12 -alkyl, or substituted or unsubstituted 3- to 13-membered carbocyclyl-C 1-12 -alkyl; R 3S is hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 ; each instance of R a is independently hydrogen, substituted or unsubstituted C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and Ring M is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; wherein Ring M is optionally substituted with R 9 as valency permits; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; and q is an integer selected from 0, 1, 2, 3, and 4. [0559] In some embodiments, R 2S is cyclopropyl or difluoromethyl. In some embodiments, R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2S is cyclopropyl or difluoromethyl; and R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . [0560] In some embodiments, a compound of Formula (V) is a compound of Formula (I) or Formula (II). [0561] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, starting with a compound of Formula (V-a4). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-rm) with a compound of Formula (V-a4). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-rm) with a compound of Formula (V-a4), via reaction a5. [0562] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a4), or salt thereof, starting from a compound of Formula (V-a3). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a4), or salt thereof, comprising reacting a compound of Formula (V-a3) with hydroxide ion. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a4), or salt thereof, comprising reacting a compound of Formula (V-a3) with hydroxide ion, via reaction a4. [0563] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, starting with a compound of Formula (V-a2). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, comprising reacting a compound of Formula (V-a2) with acetic anhydride or acetyl chloride. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, comprising reacting a compound of Formula (V-a3) with acetic anhydride or acetyl chloride, via reaction a3. [0564] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a2), or salt thereof, starting with a compound of Formula (V-a1). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a2), or salt thereof, comprising reacting a compound of Formula (V-a1) with a compound of Formula (V-h) or (V-o). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, comprising reacting a compound of Formula (V-a3) with a compound of Formula (V-h) or (V-o), via reaction a2. [0565] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a1), or salt thereof, starting with a compound of Formula (V-y). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a1), or salt thereof, starting with a compound of Formula (V-p1). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a1), or salt thereof, comprising reacting a compound of Formula (V-y) with a compound of Formula (V-p1). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a1), or salt thereof, comprising reacting a compound of Formula (V-y) with a compound of Formula (V-p1), via reaction a1. [0566] In some embodiments, reaction a1 further comprises a catalyst. In some embodiments, reaction a1 comprises a metal catalyst. In some embodiments, reaction a1 further comprises a base. In some embodiments, reaction a1 further comprises a solvent. In some embodiments, reaction a1 further comprises a solvent and catalyst. In some embodiments, reaction a1 further comprises a solvent, base, and catalyst. In some embodiments, reaction a1 further comprises elevated temperature. [0567] In some embodiments, reaction a2 further comprises an acid. In some embodiments, reaction a2 further comprises a solvent. In some embodiments, reaction a2 further comprises a solvent and acid. [0568] In some embodiments, reaction a3 further comprises elevated temperature. [0569] In some embodiments, reaction a4 further comprises a solvent. [0570] In some embodiments, reaction a5 further comprises a catalyst. In some embodiments, reaction a5 further comprises a solvent. In some embodiments, reaction a5 further comprises a solvent and catalyst.
Route B [0571] In some embodiments, a compound of Formula (V) is synthesized via Route B, wherein: X is halo; R* is hydrogen or C 1-4 alkyl, or wherein two instances of R* are joined to form a 3- to 13- membered carbocyclyl or 6- to 12-membered aryl ring; m is an integer selected from 0 or 1; R 2S is substituted or unsubstituted C 1-12 alkyl, substituted or unsubstituted C 1-12 heteroalkyl, substituted or unsubstituted 3-to 13-membered heterocyclyl, or substituted or unsubstituted 3- to 13- membered carbocyclyl, substituted or unsubstituted 3-to 13-membered heterocyclyl-C 1-12 -alkyl, or substituted or unsubstituted 3- to 13-membered carbocyclyl-C 1-12 -alkyl; R 3S is hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 ; each instance of R a is independently hydrogen, substituted or unsubstituted C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and Ring M is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; wherein Ring M is optionally substituted with R 9 as valency permits; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; and q is an integer selected from 0, 1, 2, 3, and 4. [0572] In some embodiments, R 2S is cyclopropyl or difluoromethyl. In some embodiments, R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2S is cyclopropyl or difluoromethyl; and R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . [0573] In some embodiments, a compound of Formula (V) is a compound of Formula (I) or Formula (II). [0574] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, starting with a compound of Formula (V-b3). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, starting with a compound of Formula (V-b2). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-b3) with a compound of Formula (V-b2). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-b3) with a compound of Formula (V-b2), via reaction b3. [0575] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b2), or salt thereof, starting with a compound of Formula (V-b1). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b2), or salt thereof, comprising reacting a compound of Formula (V-b1) with a compound of Formula (V-rm). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b1), or salt thereof, comprising reacting a compound of Formula (V-rm) with a compound of Formula (V-rm), via reaction b2. [0576] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b1), or salt thereof, starting with a compound of Formula (V-y). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b1), or salt thereof, comprising reacting a compound of Formula (V-y) with hydroxide. [0577] In some embodiments, reaction b1 further comprises solvent. [0578] In some embodiments, reaction b2 further comprises a solvent. In some embodiments, reaction b2 further comprises a base. In some embodiments, reaction b2 further comprises a solvent and a base. [0579] In some embodiments, reaction b3 further comprises a catalyst. In some embodiments, reaction a1 comprises a metal catalyst. In some embodiments, reaction b3 further comprises a base. In some embodiments, reaction b3 further comprises a solvent. In some embodiments, reaction b3 further comprises a solvent and catalyst. In some embodiments, reaction b3 further comprises a solvent, base, and catalyst. In some embodiments, reaction b3 further comprises elevated temperature. Route C [0580] In some embodiments, a compound of Formula (V-b3) is synthesized via Route C, wherein: X is halo; R* is hydrogen or C1-4 alkyl, or wherein two instances of R* are joined to form a 3- to 13- membered carbocyclyl or 6- to 12-membered aryl ring; m is an integer selected from 0 or 1; R 2S is substituted or unsubstituted C 1-12 alkyl, substituted or unsubstituted C 1-12 heteroalkyl, substituted or unsubstituted 3-to 13-membered heterocyclyl, or substituted or unsubstituted 3- to 13- membered carbocyclyl, substituted or unsubstituted 3-to 13-membered heterocyclyl-C 1-12 -alkyl, or substituted or unsubstituted 3- to 13-membered carbocyclyl-C 1-12 -alkyl; R 3S is hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 ; and each instance of R a is independently hydrogen, substituted or unsubstituted C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom. [0581] In some embodiments, R 2S is cyclopropyl or difluoromethyl. In some embodiments, R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2S is cyclopropyl or difluoromethyl; and R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . [0582] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b3), or salt thereof, starting with a compound of Formula (V-bii). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, comprising reacting a compound of Formula (V-bii) with a compound of Formula (V-biii). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, comprising reacting a compound of Formula (V-bii) with a compound of Formula (V-biii), via reaction b6. [0583] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b3ii), or salt thereof, starting with a compound of Formula (V-b3i). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b3ii), or salt thereof, comprising reacting a compound of Formula (V-b3i) with acetic anhydride or acetyl chloride. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-a3), or salt thereof, comprising reacting a compound of Formula (V-b3i) with acetic anhydride or acetyl chloride, via reaction b5. [0584] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- b3i), or salt thereof, starting with a compound of Formula (V-p2). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- b3i), or salt thereof, starting with a compound of Formula (V-h) or (V-o). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b3i), or salt thereof, comprising reacting a compound of Formula (V-p2) with a compound of Formula (V-h) or (V-o). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-b3i), or salt thereof, comprising reacting a compound of Formula (V-p2) with a compound of Formula (V-h) or (V-o), via reaction b4. [0585] In some embodiments, reaction b4 further comprises an acid. In some embodiments, reaction b4 further comprises a reducing agent. [0586] In some embodiments, reaction b5 further comprises elevated temperature. [0587] In some embodiments, reaction b6 further comprises a catalyst. In some embodiments, reaction b6 comprises a metal catalyst. In some embodiments, reaction b6 further comprises a base. In some embodiments, reaction b6 further comprises a solvent. In some embodiments, reaction b6 further comprises a solvent and catalyst. In some embodiments, reaction b6 further comprises a solvent, base, and catalyst. In some embodiments, reaction b6 further comprises elevated temperature.
Route D [0588] In some embodiments, a compound of Formula (V) is synthesized via Route D, wherein: X is halo; R* is hydrogen or C1-4 alkyl, or wherein two instances of R* are joined to form a 3- to 13- membered carbocyclyl or 6- to 12-membered aryl ring; m is an integer selected from 0 or 1; R 2S is substituted or unsubstituted C 1-12 alkyl, substituted or unsubstituted C 1-12 heteroalkyl, substituted or unsubstituted 3-to 13-membered heterocyclyl, or substituted or unsubstituted 3- to 13- membered carbocyclyl, substituted or unsubstituted 3-to 13-membered heterocyclyl-C 1-12 -alkyl, or substituted or unsubstituted 3- to 13-membered carbocyclyl-C 1-12 -alkyl; R 3S is hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 ; each instance of R a is independently hydrogen, substituted or unsubstituted C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and Ring M is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; wherein Ring M is optionally substituted with R 9 as valency permits; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; and q is an integer selected from 0, 1, 2, 3, and 4. [0589] In some embodiments, R 2S is cyclopropyl or difluoromethyl. In some embodiments, R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2S is cyclopropyl or difluoromethyl; and R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . [0590] In some embodiments, a compound of Formula (V) is a compound of Formula (I) or Formula (II). [0591] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, starting with a compound of Formula (V-d5). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-d5) with acetic anhydride or acetyl chloride. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-d5) with acetic anhydride or acetyl chloride via reaction d5. [0592] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d5), or salt thereof, starting with a compound of Formula (V-d4). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d5), or salt thereof, comprising reacting a compound of Formula (V-d4) with a compound of Formula (V-h) or (V-o). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d5), or salt thereof, comprising reacting a compound of Formula (V-d4) with a compound of Formula (V-h) or (V-o), via reaction d4. [0593] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d4), or salt thereof, starting with a compound of Formula (V-d2). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d4), or salt thereof, comprising reacting a compound of Formula (V-d2) with a compound of Formula (V-d3). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d4), or salt thereof, comprising reacting a compound of Formula (V-d2) with a compound of Formula (V-d3), via reaction d3. [0594] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d2), or salt thereof, starting with a compound of Formula (V-d1). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d2), or salt thereof, comprising reacting a compound of Formula (V-d1) with a compound of Formula (V-rm). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d2), or salt thereof, comprising reacting a compound of Formula (V-d1) with a compound of Formula (V-rm), via reaction d2. [0595] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d1), or salt thereof, starting with a compound of Formula (V-y). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d1), or salt thereof, comprising reacting a compound of Formula (V-y) with hydroxide. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-d1), or salt thereof, comprising reacting a compound of Formula (V-y) with hydroxide, via reaction d1. [0596] In some embodiments, reaction d1 further comprises a solvent. [0597] In some embodiments, reaction d2 further comprises a catalyst. In some embodiments, reaction d2 further comprises a solvent. In some embodiments, reaction d2 further comprises a solvent and catalyst. [0598] In some embodiments, reaction d3 further comprises a catalyst. In some embodiments, reaction d3 comprises a metal catalyst. In some embodiments, reaction d3 further comprises a base. In some embodiments, reaction d3 further comprises a solvent. In some embodiments, reaction d3 further comprises a solvent and catalyst. In some embodiments, reaction d3 further comprises a solvent, base, and catalyst. In some embodiments, reaction d3 further comprises elevated temperature. [0599] In some embodiments, reaction d4 further comprises an acid. In some embodiments, reaction d4 further comprises a solvent. In some embodiments, reaction d4 further comprises a solvent and acid. [0600] In some embodiments, reaction d5 further comprises elevated temperature.
Route E [0601] In some embodiments, a compound of Formula (V) is synthesized via Route E, wherein: X is halo; R* is hydrogen or C1-4 alkyl, or wherein two instances of R* are joined to form a 3- to 13- membered carbocyclyl or 6- to 12-membered aryl ring; m is an integer selected from 0 or 1; R 2S is substituted or unsubstituted C 1-12 alkyl, substituted or unsubstituted C 1-12 heteroalkyl, substituted or unsubstituted 3-to 13-membered heterocyclyl, or substituted or unsubstituted 3- to 13- membered carbocyclyl, substituted or unsubstituted 3-to 13-membered heterocyclyl-C 1-12 -alkyl, or substituted or unsubstituted 3- to 13-membered carbocyclyl-C 1-12 -alkyl; R 3S is hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 ; each instance of R a is independently hydrogen, substituted or unsubstituted C 1-6 alkyl, an oxygen protecting group when attached to an oxygen atom, or a nitrogen protecting group when attached to a nitrogen atom; and Ring M is 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12- membered aryl, or 5- to 14-membered heteroaryl ring; wherein Ring M is optionally substituted with R 9 as valency permits; each instance of R 9 is independently hydrogen, halogen, substituted or unsubstituted C 1-6 alkyl, –OR a , –COOR a , –COR a , –N(R a ) 2 , –CN, or –(C=O)N(R a ) 2 , or two instances of R 9 are joined to form a 3- to 13-membered carbocyclyl, 3- to 13-membered heterocyclyl, 6- to 12-membered aryl, or 5- to 14-membered heteroaryl ring; and q is an integer selected from 0, 1, 2, 3, and 4. [0602] In some embodiments, R 2S is cyclopropyl or difluoromethyl. In some embodiments, R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . In some embodiments, R 2S is cyclopropyl or difluoromethyl; and R 3S is chloro, fluoro, -CN, -CF 3 , or -COOCH 3 . [0603] In some embodiments, a compound of Formula (V) is a compound of Formula (I) or Formula (II). [0604] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, starting with a compound of Formula (V-e5). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-e5) with acetic anhydride or acetyl chloride. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V), or salt thereof, comprising reacting a compound of Formula (V-e5) with acetic anhydride or acetyl chloride via reaction e5. [0605] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-e5), or salt thereof, starting with a compound of Formula (V-e4). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-e5), or salt thereof, comprising reacting a compound of Formula (V-e4) with a compound of Formula (V-h) or (V-o). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-e5), or salt thereof, comprising reacting a compound of Formula (V-e4) with a compound of Formula (V-h) or (V-o), via reaction e4. [0606] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-e4), or salt thereof, starting with a compound of Formula (V-e3). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- e4), or salt thereof, comprising reacting a compound of Formula (V- e3) with a compound of Formula (V-rm). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- e4), or salt thereof, comprising reacting a compound of Formula (V- e3) with a compound of Formula (V-rm), via reaction e3. [0607] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-e3), or salt thereof, starting with a compound of Formula (V-e2). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- e3), or salt thereof, comprising reacting a compound of Formula (V- e2) with hydroxide. In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- e3), or salt thereof, comprising reacting a compound of Formula (V- e2) with hydroxide, via reaction e2. [0608] In some embodiments, provided herein is a method of synthesizing a compound of Formula (V-e2), or salt thereof, starting with a compound of Formula (V-y). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- e2), or salt thereof, comprising reacting a compound of Formula (V-y) with a compound of Formula (V-e1). In some embodiments, provided herein is a method of synthesizing a compound of Formula (V- e2), or salt thereof, comprising reacting a compound of Formula (V-y) with a compound of Formula (V-e1), via reaction e1. [0609] In some embodiments, reaction e1 further comprises a catalyst. In some embodiments, reaction e1 comprises a metal catalyst. In some embodiments, reaction e1 further comprises a base. In some embodiments, reaction e1 further comprises a solvent. In some embodiments, reaction e1 further comprises a solvent and catalyst. In some embodiments, reaction e1 further comprises a solvent, base, and catalyst. In some embodiments, reaction e1 further comprises elevated temperature. [0610] In some embodiments, reaction e2 further comprises a solvent. [0611] In some embodiments, reaction e3 further comprises a catalyst. In some embodiments, reaction e3 further comprises a solvent. In some embodiments, reaction e3 further comprises a solvent and catalyst. [0612] In some embodiments, reaction e4 further comprises an acid. In some embodiments, reaction e4 further comprises a solvent. In some embodiments, reaction e4 further comprises a solvent and acid. [0613] In some embodiments, reaction e5 further comprises elevated temperature. EXAMPLES [0614] In order that the present disclosure may be more fully understood, the following examples are set forth. The synthetic and biological examples described in this Application are offered to illustrate the compounds, pharmaceutical compositions, methods, and uses provided herein and are not to be construed in any way as limiting their scope. [0615] The following abbreviations are used: aqueous (aq.) acetonitrile (ACN); dichloromethane (DCM); dichloroethane (DCE); ethyl acetate (EtOAc); flash column chromatography (fcc); hours (h); minutes (min); reaction mixture (RM); retention time (Rt); room temperature (RT). [0616] NMR chemical shifts, δ, are given in ppm Example 1. Synthesis [0617] Exemplary synthetic procedures are provided herein. The schemes described cover all compounds provided herein. The reactions are versatile and order of the steps can be changed accordingly. LC-MS methods (analytical and preparative) Methods 1, 2, 3, 4, 8, 9 [0618] LC/MS System: Acquity UPLC coupled with SQD or QDA mass spectrometer [0619] Column. Column for Methods 1, 2, 8, 9: Acquity UPLC BEH C18 (2.1 mm x 50 mm i.d., 1.7 μm packing diameter). Column for Methods 3 and 4: Acquity UPLC BEH C18 (2.1 mm x 100 mm i.d., 1.7 μm packing diameter) [0620] Mobile phase. Low pH: A: 0.1% formic acid in water and B: 0.1% formic acid in ACN. High pH: A: 0.05% ammonia in water and B: 0.05% ammonia in ACN. [0621] Column temperature: 40 °C. [0622] UV detection: 210-400 nm. [0623] MS. Ionisation Mode: Electrospray Positive and Negative (ES+/ES-). Scan Range: 100-1500 Da (for SQD); 1250 Da (for Qda).. Methods 5 and 5b [0624] LC/MS System. Agilent 1290 Infinity II LC/SFC coupled with Agilent Technologies 6540 UHD Q-ToF Mass Spectrometer. [0625] Column. Acquity UPLC BEH C18 (2.1 mm x 100 mm i.d., 1.7 μm packing diameter). [0626] Mobile phase- Method 5. A= 0.1% formic acid in water and B= 0.1% formic acid in ACN. [0627] Mobile phase- Method 5b: A= 0.1% formic acid in water, mobile phase B= ACN [0628] UV detection: 190-400 nm. [0629] Column temperature: 40 °C. MS. Ionisation Mode: alternate-scan Positive and Negative Electrospray (ES+/ES-). Ion Source: ESI, Spray Voltage: 3500V. Scan Range: 100-1700 Da. [0630] Methods 5 and 5b: 0.1% formic acid in water, mobile phase B: ACN [0631] Preparative LC-MS methods: gradients used covered the range from 3-97% ACN. Preparative LC-MS Methods (Methods 6 and 7) [0632] LC/MS System: Agilent 1260 Infinity II (LC/MSD). [0633] Column. Method 6: XBridge C18 (30 x 150mm, 5 μm). Method 7: SunFire C18 (30 x 150mm, 5 μm). [0634] Mobile phase. Method 6 - high pH: A: 0.025% ammonia in water and B: ACN. Method 7 - low pH: A: 0.1% formic acid in water and B: ACN. [0635] Column temperature: ambient. [0636] UV detection: 200-350 nm. [0637] MS: Ionisation Mode: alternate-scan Positive and Negative Electrospray (ES+/ES-). Scan Range: 100-1000 Da.
Scheme 1 Scheme 1 with procedures 2a/3a Step 1: Methyl 6-(4-amino-3-chloro-phenyl)pyridine-3-carboxylate [0638] To a mixture of methyl 6-bromopyridine-3-carboxylate (208 g, 963 mmol), carboxylate 2- chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)anilin e (256 g, 1011 mmol) and K 3 PO 4 (635 g, 2994 mmol), were added dioxane (4500 mL) and H 2 O (400 mL). The RM was degassed with N 2 for 15 min and Pd(Ph 3 P) 4 (5.0 g) was added. It was stirred at 65 °C for 27h, then cooled to 25 °C. EtOAc (3L), conc. aq. NaHCO 3 (1L) and H 2 O (1L) were added. The layers were separated. The H 2 O layer was extracted with EtOAc (1L). The organic layers were gathered and washed with brine (2L). The organic layers were concentrated to about 0.5 L during which a yellow suspension was formed. n- Heptane (1.5 L) was added and the suspension was stirred for 30 min. It was filtered, dried under reduced pressure for 45 min, then under vacuum at 45 °C for 16h to yield a yellow solid (221 g). The crude product was triturated in DCM (2L) for 30 min, n-heptane (1.5 L) was added. The suspension was concentrated to about 2L and cooled to RT. The solid was filtered, dried under reduced pressure for 30 min, then under vacuum at 45 °C for 18h to yield methyl 6-(4-amino-3-chloro-phenyl)pyridine- 3-carboxylate as a yellow solid (195 g, 77%). LC-MS (Method 1): Rt = 1.74 min; m/z = 263.15 [M+H] + . 1 H-NMR (400 MHz, DMSO-d 6 ): δ 3.87 (s, 3H), 5.91 (bs, 2H), 6.87 (d, J = 8.49 Hz, 1H), 7.88 (dd, J = 8.39, 2.12 Hz, 1H), 7.95 (dd, J = 8.5, 0.8 Hz, 1H), 8.06 (d, J = 2.08 Hz, 1H), 8.21 (dd, J = 8.4, 2.3 Hz, 1H), 9.02 (dd, J = 2.3, 0.8 Hz, 1H). Step 2a: Methyl 6-[3-chloro-4-(cyclopropylmethylamino)phenyl]pyridine-3-carb oxylate [0639] To a suspension of methyl 6-(4-amino-3-chloro-phenyl)pyridine-3-carboxylate (45 g, 170 mmol) in 1,2- dichloroethane (1350 mL), were added cyclopropanecarbaldehyde (15.2 mL, 204 mmol) and CH 3 CO 2 H (2.23 mL, 39 mmol) under N 2 atmosphere. Molecular sieves 4Å (20 g) were added and the RM was stirred at RT for 2h. It was cooled to 0 °C and NaBH(CH 3 COO) 3 (55 g, 113 mmol) was added in small portions during 30 min. The RM was then left to stir at RT overnight. It was diluted with DCM and washed with NaHCO 3 and brine. The organic layer was dried, then concentrated in vacuo to afford the crude product. The latter was triturated with ACN. The resulting solid was filtered, washed with ACN and dried to afford methyl 6-[3-chloro-4- (cyclopropylmethylamino)phenyl]pyridine-3-carboxylate (44.2 g, 68%). LC-MS (Method 4): Rt = 5.71 min, m/z = 317.1 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.22-0.3 (m, 2H), 0.42-0.5 (m, 2H), 1.05-1.19 (m, 1H), 3.11 (m, 2H), 3.87 (s, 3H), 5.85 (t, J = 5.73 Hz, 1H), 6.87 (d, J = 8.77 Hz, 1H), 7.95-8.02 (m, 2H), 8.12 (d, J = 2.19 Hz, 1H), 8.22 (dd, J = 8.43, 2.36 Hz, 1H), 9.04 (dd, J = 2.25, 0.7 Hz, 1H). Step 3a: Methyl 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]pyridi ne-3-carboxylate [0640] To a suspension of methyl 6-[3-chloro-4-(cyclopropylmethylamino)phenyl]pyridine-3- carboxylate (44.16 g, 139 mmol) in dry dichloromethane (770 mL), previously cooled in an ice bath, was slowly added acetyl chloride (39.6 mL, 558 mmol) over 15 min. The RM was left to stir for at RT for 3h. It was diluted with water, then treated with 10% NaOH to pH = 8. The layers were separated; the organic layer was washed with brine, dried over anh. Na2SO4 and concentrated under reduced pressure to afford methyl 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]pyridi ne-3- carboxylate (51.6 g, 99%). LC-MS (Method 4): Rt = 4.81 min, m/z = 359.10 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.001-0.06 (m, 2H), 0.32-0.42 (m, 2H), 0.81-0.94 (m, 1H), 1.73 (s, 3H), 3.18- 3.28 (m, 1H), 3.68-3.77 (m, 1H), 3.91 (s, 3H), 7.68 (d, J = 8.35 Hz, 1H), 8.21-8.29 (m, 2H), 8.39 (dd, J = 8.34, 2.24 Hz, 1H), 8.41 (d, J = 2.1 Hz, 1H), 9.17 (dd, J = 2.23, 0.87 Hz, 1H). Step 4: 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]pyridi ne-3-carboxylic acid [0641] To a solution of methyl 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]pyridi ne-3- carboxylate (51.56 g, 139 mmol) in a mixture of THF (542 mL) and water (203 mL), was added LiOH (10 g, 418 mmol). The resulting solution was stirred at RT overnight. The RM was extracted with EtOAc. The aqueous layer was acidified to pH = 4 with 1N HCl and then stirred vigorously with cooling in an ice bath. The resulting precipitate was filtered, washed with water and ACN. It was recrystallized from acetone (350 mL) to yield 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro- phenyl]pyridine-3-carboxylic acid as a yellow foam (32.7 g, 67%). LC-MS (Method 3): Rt = 3.88 min, m/z = 345.00 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.001-0.06 (m, 2H), 0.32-0.42 (m, 2H), 0.80-0.94 (m, 1H), 1.73 (s, 3H), 3.18-3.27 (m, 1H), 3.68-3.78 (m, 1H), 7.67 (d, J = 8.45 Hz, 1H), 8.19-8.28 (m, 2H), 8.36 (dd, J = 8.36, 2.23 Hz, 1H), 8.41 (d, J = 2.0 Hz, 1H), 9.15 (dd, J = 2.19, 0.76 Hz, 1H), 13.5 (bs, 1H). Step 5: 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (1H-pyrazol-4-yl)ethyl] pyridine-3-carboxamide (I-T2111) [0642] To a solution of 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]pyridi ne-3- carboxylic acid (50.0 mg, 0.145 mmol) in dry DCM (3.5 mL), were added HATU (0.0662 g, 0.174 mmol) and DIPEA (0.0248 mL, 0.145 mmol) were added. The RM was stirred at RT for 30 min.2- (1H-pyrazol-4-yl)ethanamine (0.0193 g, 0.174 mmol) was added and stirring was continued at RT. The reaction was quenched with sat. NaHCO 3 , the layers were separated, and the organic layer was washed with brine, dried and evaporated. Purification by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (1H-pyrazol-4- yl)ethyl]pyridine-3-carboxamide as a white solid (30 mg, 47%). LC-MS (Method 4): Rt = 3.55 min; m/z = 438.04 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01-0.08 (m, 2H), 0.37-0.42 (m, 2H), 0.83- 0.94 (m, 1H), 1.74 (s, 3H), 2.71-2.81 (m, 2H), 3.20-3.27 (m, 1H), 3.44-3.48 (m, 2H), 3.74 (m, 1H), 7.45 (br d, J = 4.27 Hz, 2H), 7.67 (br d, J = 8.24 Hz, 1H), 8.20-8.28 (m, 2H), 8.30 (m, 1H), 8.35-8.44 (m, 1H), 8.82 (m, 1H), 9.10 (br s, 1H), 12.6 (br s, 1H). Following Scheme 1: procedures 2a/3a 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-ben zyl-pyridine-3-carboxamide (I- T2113) [0643] Purification by fcc (silica gel, 5% MeOH in EtOAc) afforded 6-[4-[acetyl(cyclopropylmethyl) amino]-3-chloro-phenyl]-N-benzyl-pyridine-3-carboxamide as a white solid (37 mg, 56%). LC-MS (Method 4): Rt = 4.64 min; m/z = 434.07 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.08 (m, 2H), 0.32-0.39 (m, 2H), 0.82-0.93 (m, 1H), 1.73 (s, 3H), 3.18-3.28 (m, 1H), 3.68-3.77 (m, 1H), 4.52 (d, J = 5.86 Hz, 2H), 7.21-7.29 (m, 1H), 7.31-7.37 (m, 4H), 7.66 (d, J = 8.23 Hz, 1H), 8.23 (d, J = 8.19 Hz, 2H), 8.36 (dd, J = 8.51, 2.49 Hz, 1H), 8.39 (d, J = 1.97 Hz, 1H), 9.15 (d, J = 1.97 Hz, 1H), 9.31 (t, J = 5.83 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(te trahydrofuran-3-ylmethyl) pyridine-3-carboxamide (I-T2115) [0644] Purification by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropylmethyl) amino]-3-chloro-phenyl]-N-(tetrahydrofuran-3-ylmethyl)pyridi ne-3-carboxamide as a white solid (41 mg, 43%). LC-MS (Method 4): Rt = 3.76 min; m/z = 427.96 [M+H] + . 1 H-NMR (300 MHz, DMSO- d 6 ): δ 0.01-0.07 (m, 2H), 0.32-0.41 (m, 2H), 0.80-0.94 (m, 1H), 1.55-1.66 (m, 1H), 1.73 (s, 3H), 1.90- 2.01 (m, 1H), 3.18-3.26 (m, 3H), 3.46-3.51 (m, 1H), 3.58-3.78 (m, 5H), 7.66 (d, J = 8.26 Hz, 1H), 8.19-8.24 (m, 2H), 8.27-8.32 (m, 1H), 8.39 (d, J = 2.05 Hz, 1H), 8.83 (t, J = 5.65 Hz, 1H), 9.10 (d, J = 2.19 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(1H -pyrazol-3-ylmethyl)pyridine-3- carboxamide (I-T2132) [0645] Purification by preparative HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl) amino]-3-chloro-phenyl]-N-(1H-pyrazol-3-ylmethyl)pyridine-3- carboxamide as a white foam (22 mg, 35%). LC-MS (Method 5): Rt = 5.03 min; m/z = 424.13 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.03-0.08 (m, 2H), 0.31-0.43 (m, 2H), 0.81-0.96 (m, 1H), 1.73 (s, 3H), 3.18-3.27 (m, 1H), 3.67-3.78 (m, 1H), 4.5 (d, J = 4.5 Hz, 2H), 6.19 (d, J = 2.09 Hz, 1H), 7.57 (d, J = 1.86 Hz, 1H), 7.65 (d, J = 8.25 Hz, 1H), 8.18-8.25 (m, 2H), 8.31-8.37 (m, 1H), 8.39 (d, J = 1.9 Hz, 1H), 9.13 (d, J = 1.7 Hz, 1H), 9.2 (t, J = 5.3 Hz, 1H), 12.62 (bs, 1H). N-[2-Chloro-4-[5-(1,3-dihydropyrrolo[3,4-c]pyridine-2-carbon yl)-2-pyridyl]phenyl]-N- (cyclopropylmethyl)acetamide (I-T2138) [0646] Purification by fcc (silica gel, 10% MeOH in DCM) afforded N-[2-chloro-4-[5-(1,3- dihydropyrrolo[3,4-c]pyridine-2-carbonyl)-2-pyridyl]phenyl]- N-(cyclopropylmethyl)acetamide as a white solid (22 mg, 42%). LC-MS (Method 4): Rt = 3.72 min; m/z = 447.00 [M+H] + . 1 H-NMR (400 MHz, DMSO-d 6 , 80 °C): δ 0.01-0.12 (m, 2H), 0.36-0.45 (m, 2H), 0.88-0.98 (m, 1H), 1.76 (bs, 3H), 3.22-3.33 (m, 1H), 3.72-3.80 (m, 1H), 4.89.4-98 (m, 4H), 7.32-7.42 (m, 1H), 7.70 (d, J = 8.32 Hz, 1H), 8.17-8.24 (m, 3H), 8.35 (bs, 1H), 8.48 (d, J = 5.11 Hz, 1H), 8.52-8.62 (m, 1H), 8.94 (s, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(py ridazin-3-ylmethyl)pyridine-3- carboxamide (I-T2146) [0647] Purification by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(pyridaz in-3-ylmethyl)pyridine-3- carboxamide as a white solid (20 mg, 43%). LC-MS (Method 4): Rt = 3.43 min; m/z = 435.99 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.08 (m, 2H), 0.31-0.41 (m, 2H), 0.81-0.94 (m, 1H), 1.73 (s, 3H), 3.17-3.27 (m, 1H), 3.70-3.77 (m, 1H), 4.80 (d, J = 5.75 Hz, 2H), 7.64-7.70 (m, 3H), 8.20-8.27 (m, 2H), 8.34-8.42 (m, 2H), 9.12-9.19 (m, 2H), 9.50 (t, J = 5.70 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (3-pyridyl)ethyl]pyridine-3- carboxamide (I-T2130) [0648] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[2-(3-pyridyl)ethyl]pyridine-3-carboxamide as a white solid (31 mg, 57%). LC-MS (Method 3): Rt = 2.91; m/z = 449.05 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01 - 0.07 (m, 2H), 0.34-0.43 (m, 2H), 0.85 - 0.92 (m, 1H), 1.74 (s, 3H), 2.90 (t, J = 6.93, 2H), 3.20-3.26 (m, 1H), 3.53- 3.59 (m, 2H), 3.71-3.76 (m, 1H), 7.32 (dd, J = 7.73, 4.78 Hz, 1H), 7.65-7.70 (m, 2H), 8.20 - 8.24 (m, 2H), 8.26 (dd, J = 8.44, 2.43 Hz, 1H), 8.39 (d, J = 1.92 Hz, 1H), 8.42 (d, J = 4.73 Hz, 1H), 8.47 (d, J = 1.87 Hz, 1H), 8.84 (t, J = 5.67 Hz, 1H), 9.05 (d, J = 1.94 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(py rimidin-5-ylmethyl)pyridine-3- carboxamide (I-T2147) [0649] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(pyrimidin-5-ylmethyl)pyridine-3-carboxamid e as a white solid (19 mg, 38%). LC- MS (Method 3): Rt = 3.52 min; m/z = 436.03 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01-0.07 (m, 2H), 0.33-0.41 (m, 2H), 0.85-0.93 (m, 1H), 1.74 (s, 3H), 3.20-3.27 (m, 1H,), 3.71-3.77 (m, 1H), 4.55 (d, J = 5.76 Hz, 2H), 7.67 (d, J = 8.11 Hz, 1H), 8.22-8.26 (m, 2H), 8.35 (dd, J = 8.35, 2.46 Hz, 1H), 8.40 (d, J = 2.19, 1H), 8.81 (s, 2H), 9.10 (s, 1H), 9.14 (d, J = 2.05 Hz, 1H), 9.37 (t, J = 5.65 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(py razin-2-ylmethyl)pyridine-3- carboxamide (I-T2154) [0650] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(pyrazin-2-ylmethyl)pyridine-3-carboxamide as a white solid (8 mg, 17%). LC-MS (Method 3): Rt = 3.62 min; m/z = 436.03 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01-0.07 (m, 2H), 0.34-0.42 (m, 2H), 0.85-0.93 (m, 1H), 1.74 (s, 3H), 3.20-3.26 (m, 1H), 3.71-3.78 (m, 1H), 4.67 (d, J = 5.77 Hz, 2H), 7.67 (d, J = 8.40 Hz, 1H), 8.23-8.26 (m, 2H), 8.37 (dd, J = 8.32, 2.22 Hz, 1H), 8.41 (d, J = 1.86 Hz, 1H), 8.56 (d, J = 2.51 Hz, 1H), 8.61 (m, 1H), 8.70 (m, 1H), 9.16 (d, J =1.82 Hz, 1H), 9.46 (t, J = 5.77 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-imi dazo[1,2-a]pyrimidin-6-yl- pyridine-3-carboxamide (I-T2140) [0651] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-imidazo[1,2-a]pyrimidin-6-yl-pyridine-3-car boxamide as a white foam (18 mg, 32%). LC-MS (Method 5): Rt = 4.52 min; m/z = 461.08 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.003-0.06 (m, 2H), 0.32-0.42 (m, 2H), 0.83-0.95 (m, 1H), 1.74 (s, 3H), 3.18–3.26 (m, 1H,), 3.69-3.79 (m, 1H), 7.66-7.73 (m, 2H), 8.04 (d, J = 1.32 Hz, 1H), 8.28 (dd, J = 8.36, 1.99 Hz, 1H), 8.33 (d, J = 8.16 Hz, 1H), 8.45 (d, J = 1.87 Hz, 1H), 8.49 (dd, J = 8.59, 2.3 Hz, 1H), 8.71 (d, J = 2.4 Hz, 1H), 9.25-9.28 (m, 1H), 9.68 (d, J = 2.6 Hz, 1H), 10.87 (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(3- thienylmethyl)pyridine-3- carboxamide (I-T2149) [0652] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(3-thienylmethyl)pyridine-3-carboxamide as a white foam (3.7 mg, 7%). LC-MS (Method 5): Rt = 6.15 min; m/z = 440.06 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01-0.07 (m, 2H), 0.33-0.42 (m, 2H), 0.80-0.93 (m, 1H), 1.74 (s, 3H), 3.21-3.27 (m, 1H,), 3.71-3.77 (m, 1H), 4.52 (d, J = 5.76 Hz, 2H), 7.12 (d, J = 4.76 Hz, 1H), 7.37-7.39 (m, 1H), 7.49-7.51 (m, 1H), 7.67 (d, J = 8.12, 1H), 8.21-8.25 (m, 2H), 8.35 (dd, J = 8.54, 2.24 Hz, 1H), 8.4 (d, J = 1.82 Hz, 1H), 9.14-9.16 (m, 1H), 9.25 (t, J = 5.8 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(1H -imidazol-4-ylmethyl)pyridine- 3-carboxamide (I-T2151) [0653] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(1H-imidazol-4-ylmethyl)pyridine-3-carboxam ide as a white foam (14.7 mg, 29%). LC-MS (Method 5): Rt = 4.04 min; m/z = 424.09 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01- 0.07 (m, 2H), 0.32-0.40 (m, 2H), 0.82-0.95 (m, 1H), 1.73 (s, 3H), 3.17-3.27 (m, 1H), 3.67-3.77 (m, 1H), 4.36-4.47 (m, 2H), 6.95-7.03 (m, 1H), 7.5-7.59 (m, 1H), 7.66 (d, J = 8.57 Hz, 1H), 8.18-8.25 (m, 2H), 8.34 (dd, J = 8.31, 2.2 Hz, 1H), 8.39 (d, J = 1.9 Hz, 1H), 9.03-9.15 (m, 2H), 11.90 (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-ind an-2-yl-pyridine-3-carboxamide (I-T2152) [0654] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-indan-2-yl-pyridine-3-carboxamide as a white foam (11.7 mg, 21%). LC-MS (Method 5): Rt = 6.69 min; m/z = 460.13 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.007-0.06 (m, 2H), 0.31-0.41 (m, 2H), 0.83-0.94 (m, 1H), 1.73 (s, 3H), 2.92-3.03 (m, 2H), 3.17-3.3 (m, 3H), 3.68- 3.78 (m, 1H), 4.67-4.78 (m, 1H), 7.12-7.19 (m, 2H), 7.20-7.26 (m, 2H), 7.66 (d, J = 8.26 Hz, 1H), 8.18-8.25 (m, 2H), 8.3 (dd, J = 8.4, 2.3 Hz, 1H), 8.39 (d, J = 1.96 Hz, 1H), 8.90-8.95 (m, 1H), 9.10- 9.12 (m, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(5- bromoindan-2-yl)pyridine-3- carboxamide (I-T2153) [0655] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(5-bromoindan-2-yl)pyridine-3-carboxamide as a white foam (12.4 mg, 18%). LC- MS (Method 4): Rt = 5.42 min; m/z = 539.82 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.05 (m, 2H), 0.32-0.42 (m, 2H), 0.82-0.94 (m, 1H), 1.73 (s, 3H), 2.95 (dt, J = 17.35, 6.16 Hz, 2H), 3.17- 3.29 (m, 3H,), 3.68-3.78 (m, 1H), 4.68-4.77 (m, 1H), 7.21 (d, J = 8.32 Hz, 1H), 7.34 (dd, J = 7.89. 1.58 Hz, 1H), 7.43-7.46 (m, 1H), 7.66 (d, J = 8.26 Hz, 1H), 8.18-8.25 (m, 2H), 8.32 (dd, J = 8.32, 2.15 Hz, 1H), 8.39 (d, J = 2.1 Hz, 1H), 8.93 (d, J = 6.6 Hz, 1H), 9.08-9.12 (m, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(1H -pyrrol-2-ylmethyl)pyridine-3- carboxamide (I-T2121) [0656] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(1H-pyrrol-2-ylmethyl)pyridine-3-carboxamid e as a white foam (19.4 mg, 31%). LC-MS (Method 3): Rt = 4.24 min; m/z = 423.13 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01- 0.06 (m, 2H), 0.33-0.40 (m, 2H), 0.82-0.94 (m, 1H), 1.73 (s, 3H), 3.17-3.26 (m, 1H), 3.68-3.78 (m, 1H), 4.45 (d, J = 5.44 Hz, 2H), 5.91-5.97 (m, 2H), 6.63-6.67 (m, 1H), 7.65 (d, J = 8.24 Hz, 1H), 8.19- 8.24 (m, 2H), 8.34 (dd, J = 8.31, 2.23 Hz, 1H), 8.39 (d, J = 1.94 Hz, 1H), 9.08 (t, J = 5.58 Hz, 1H), 9.13 (d, J=2.38 Hz, 1H), 10.64, (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-3-yl)propyl] pyridine-3-carboxamide (I-T2127) [0657] Purification by prep HPLC (Method 7) and additionally by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-3- yl)propyl]pyridine-3-carboxamide as a white foam (25.0 mg, 38%). LC-MS (Method 4): Rt = 3.73 min; m/z = 452.25 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.08 (m, 2H), 0.32-0.42 (m, 2H), 0.83-0.94 (m, 1H), 1.73 (s, 3H), 1.79-1.91 (m, 2H), 2.57-2.69 (m, 2H), 3.17-3.26 (m, 1H), 3.31-3.37 (m, 2H), 3.69- 3.77 (m, 1H), 6.02-6.09 (m, 1H), 7.33 (bs, 0.5H), 7.58 (bs, 0.5H), 7.66 (d, J = 8.21 Hz, 1H), 8.19-8.25 (m, 2H), 8.30 (dd, J = 8.36, 2.43 Hz, 1H), 8.39 (d, J = 1.82 Hz, 1H), 8.76 (t, J = 4.56 Hz, 1H), 9.09 (d, J = 1.97 Hz, 1H), 12.37-12.58 (m, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(3- imidazol-1-ylpropyl)pyridine-3- carboxamide (I-T2128) [0658] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(3-imidazol-1-ylpropyl)pyridine-3-carboxami de as a white foam (8.3 mg, 12%). LC-MS (Method 5): Rt = 4.23 min; m/z = 452.08 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01- 0.07 (m, 2H), 0.34-0.41 (m, 2H), 0.86-0.93 (m, 1H), 1.74 (s, 3H), 1.96-2.02 (m, 2H), 3.21-3.26 (m, 1H), 3.26-3.30 (m, 2H) 3.71-3.77 (m, 1H), 4.05 (t, J = 6.87 Hz, 2H), 6.91 (s, 1H), 7.23 (s, 1H), 7.67 (d, J = 8.24 Hz, 1H), 7.69 (s, 1H), 8.22-8.26 (m, 2H), 8.30-8.34 (m, 1H), 8.41 (d, J = 2.14 Hz, 1H), 8.77 (t, J = 5.49 Hz, 1H), 9.11 (d, J = 1.83 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (1H-imidazol-4-yl)ethyl]pyridine- 3-carboxamide (I-T2150) [0659] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[2-(1H-imidazol-4-yl)ethyl]pyridine-3-carbo xamide as a white foam (13.0 mg, 20%). LC-MS (Method 5): Rt = 4.10 min; m/z = 438.07 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01 - 0.06 (m, 2H), 0.34-0.41 (m, 2H), 0.86-0.94 (m, 1H), 1.74 (s, 3H), 2.76-2.82 (m, 2H), 3.21-3.26 (m, 1H), 3.49-3.56 (m, 2H), 3.71-3.77 (m, 1H), 6.84 (s, 1H), 7.55 (s, 1H), 7.67 (d, J = 8.24 Hz, 1H), 8.21-8.24 (m, 2H), 8.27-8.33 (m, 1H), 8.40 (d, J = 1.83 Hz, 1H), 8.84 (t, J = 5.49 Hz, 1H), 9.10 (d, J=1.83 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(1H -benzimidazol-5-yl)pyridine-3- carboxamide (I-T2137) [0660] Purification by prep HPLC (Method 7) and additionally by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(1H -benzimidazol-5- yl)pyridine-3-carboxamide as a white foam (5.5 mg, 7%). LC-MS (Method 4): Rt = 3.65 min; m/z = 460.23 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.08 (m, 2H), 0.32-0.42 (m, 2H), 0.83-0.95 (m, 1H), 1.75 (s, 3H), 3.20-3.28 (m, 1H), 3.70-3.80 (m, 1H), 7.46-7.62 (m, 2H), 7.69 (d, J = 8.36 Hz, 1H), 8.15-8.22 (m, 2H), 8.22-8.35 (m, 2H), 8.39-8.51 (m, 2H), 9.23 (bs, 1H), 10.49 (bs, 1H), 12.42 (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-4-yl)propyl] pyridine-3-carboxamide (I-T2269) [0661] Purification by fcc (silica gel, 10% MeOH in DCM), followed by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-4- yl)propyl]pyridine-3-carboxamide as a white solid (15.6 mg, 24%). LC-MS (Method 3): Rt = 3.65 min; m/z = 452.16 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.08 (m, 2H), 0.31-0.44 (m, 2H), 0.79-0.98 (m, 1H), 1.73 (s, 3H), 1.73-1.84 (m, 2H), 2.48 (m, 2H), 3.22 (dd, J = 13.9, 7.3 Hz, 1H), 3.3 (m, 2H), 3.73 (dd, J = 14.0, 7.0 Hz, 1H), 7.45 (m, 2H), 7.66 (d, J = 8.2 Hz, 1H), 8.18-8.26 (m, 2H), 8.29- 8.34 (m, 1H), 8.39 (m, 1H), 8.74 (t, J = 5.5 Hz, 1H), 9.10 (m, 1H), 12.53 (bs, 1H). N-[2-Chloro-4-[5-(isoindoline-2-carbonyl)-2-pyridyl]phenyl]- N-(cyclopropylmethyl)acetamide (I-T2270) [0662] Purification by prep HPLC (Method 6) afforded N-[2-chloro-4-[5-(isoindoline-2-carbonyl)-2- pyridyl]phenyl]-N-(cyclopropylmethyl)acetamide as a white solid (9.6 mg, 14%). LC-MS (Method 3): Rt = 4.8 min; m/z = 446.56 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.02- 0.07 (m, 2H), 0.37-0.40 (m, 2H), 0.87-0.95 (m, 1H), 1.75 (s, 3H), 3.25 (dd, J = 14.0, 7.3 Hz, 1H), 3.75 (dd, J = 14.0, 7.0 Hz, 1H), 4.90 (d, J = 12.9 Hz, 4H), 7.26-7.35 (m, 3H), 7.42-7.43 (m, 1H), 7.68 (d, J = 8.2 Hz, 1H), 8.17- 8.27 (m, 3H), 8.41 (d, J = 2.0 Hz, 1H), 8.93-8.97 (m, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-N-[2- (3-pyridyl)ethyl]pyridine-3- carboxamide (I-T2301) [0663] Purification by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-N-[2-(3-py ridyl)ethyl]pyridine-3-carboxamide (42.0 mg, 63%) as a white solid. LC-MS (Method 4): Rt = 3.66 min, m/z = 432.91 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 0.001-0.07 (m, 2H), 0.29-0.41 (m, 2H), 0.8-0.94 (m, 1H), 1.78 (s, 3H), 2.89 (t, J = 7 Hz, 2H), 3.45-3.61 (m, 4H), 7.28-7.35 (m, 1H), 7.56-7.70 (m, 2H), 8.03-8.28 (m, 4H), 8.4 (dd, J = 4.83, 1.56, 1H), 8.45-8.48 (m, 1H), 8.83 (t, J = 5.4 Hz, 1H), 9.01-9.06 (m, 1H) 6-[4-[acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-N-[3- (1H-pyrazol-4-yl)propyl]pyridine- 3-carboxamide (I-T2302) [0664] Purification by fcc (silica gel, 5% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-N-[3-(1H-p yrazol-4-yl)propyl]pyridine-3 carboxamide (14 mg, 20.6%) as a white solid. LC-MS (Method 4): Rt = 3.58 min, m/z = 435.94 [M+H] + . 1 H-NMR (600 MHz, DMSO-d 6 ): δ 0.01-0.11 (m, 2H), 0.36 (m, 2H), 0.82-0.93 (m, 1 H), 1.75-1.83 (m, 5H), 2.52 (t, J = 2.0 Hz, 2H), 3.34-3.38 (m, 2H), 3.52 (dd, J = 6.5, 4.7 Hz, 2H), 7.35 (br s, 1H), 7.54 (br s, 1H), 7.63 (t, J = 8.2 Hz, 1H), 8.11 (dd, J = 8.3, 1.7 Hz, 1H), 8.15 (dd, J = 11.5, 1.7 Hz, 1H), 8.20 (d, J = 8.3 Hz, 1H), 8.31 (dd, J = 8.3, 2.2 Hz, 1H), 8.74 (t, J = 5.5 Hz, 1H), 9.10 (d, J = 1.8 Hz, 1H), 12.52 (br s, 1H). N-[2-chloro-4-[5-(5-fluoroisoindoline-2-carbonyl)-2-pyridyl] phenyl]-N-(cyclopropylmethyl) acetamide (I-T2336) [0665] Purification by prep HPLC (Method 7) afforded N-[2-chloro-4-[5-(5-fluoroisoindoline-2- carbonyl)-2-pyridyl]phenyl]-N-(cyclopropylmethyl)acetamide as a white solid (46 mg, 49%). LC-MS (Method 3): Rt = 4.81 min, m/z = 464.06 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.09 (m, 2H), 0.35 - 0.43 (m, 2H), 0.87 - 0.95 (m, 1H), 1.75 (s, 3H), 3.25 (dd, J = 13.9, 7.3 Hz, 1H), 3.75 (dd, J = 14.0, 7.1 Hz, 1H), 4.83 - 4.93 (m, 4H), 7.11 - 7.19 (m, 1.5H), 7.27 - 7.34 (m, 1H), 7.44 - 7.47 (m, 0.5H), 7.68 (d, J = 8.3 Hz, 1H), 8.17 - 8.21 (m, 1H), 8.19 (dd, J = 8.2, 2.2 Hz, 1H), 8.41 (d, J = 2.03 Hz, 1H), 8.94 (d, J = 2.03 Hz,, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(cy clohexylmethyl)pyridine -3- carboxamide (I-T2337) [0666] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(cyclohexylmethyl)pyridine-3-carboxamide as a white solid (75.8 mg, 88%). LC- MS (Method 3): Rt = 5.19 min, m/z = 440.14 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 0.02 - 0.07 (m, 2H), 0.33 - 0.40 (m, 2H), 0.85 - 0.98 (m, 3H), 1.11 - 1.23 (m, 3H), 1.52 - 1.64 (m, 2H), 1.64 - 1.75 (m, 7 H), 3.14 (t, J = 6.2 Hz, 2H), 3.18 - 3.27 (m, 1H), 3.73 (dd, J = 14.1, 7.1Hz, 1H), 7.66 (d, J = 8.2 Hz, 1H), 8.19 - 8.24 (m, 2H), 8.27 - 8.33 (m, 1H), 8.39 (d, J = 1.8 Hz, 1H), 8.69 (t, J = 5.9 Hz, 1H), 9.07 - 9.11 (m, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(1 -methyl-3-piperidyl) methyl]pyridine-3-carboxamide (I-T2338) [0667] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[(1-methyl-3-piperidyl)methyl]pyridine-3-ca rboxamide as a white solid (56.3 mg, 63%). LC-MS (Method 3): Rt = 2.75 min, m/z = 455.03 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 0.01 - 0.06 (m, 2H), 0.33 - 0.41 (m, 2H), 0.84 - 0.95 (m, 2H), 1.36 - 1.50 (m, 1H), 1.57 - 1.69 (m, 3H), 1.73 (s, 3H), 1.77 - 1.88 (m, 2H), 2.13 (s, 3H), 2.56 - 2.64 (m, 1H), 2.68 - 2.75 (m, 1H), 3.14 - 3.26 (m, 3H), 3.73 (dd, J = 14.1, 6.9 Hz, 1H), 7.66 (d, J = 8.3Hz, 1H), 8.19 - 8.24 (m, 2H), 8.28 - 8.33 (m, 1H), 8.38-8.40 (m, 1H), 8.72 (t, J = 5.9 Hz, 1H), 9.10 (s, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (1-piperidyl)ethyl]pyridine-3- carboxamide (I-T2339) [0668] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[2-(1-piperidyl)ethyl]pyridine-3-carboxamid e as a white solid (71.2 mg, 79%). LC- MS (Method 3): Rt = 2.86 min, m/z = 455.13 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 0.02 - 0.05 (m, 2H), 0.33 - 0.40 (m, 2H), 0.84 - 0.94 (m, 1H), 1.33 - 1.41 (m, 2H), 1.45 - 1.52 (m, 4H), 1.73 (s, 3H), 2.34 - 2.41 (m, 4H), 2.44 (t, J = 7.1 Hz, 2H), 3.19 - 3.26 (m, 1H), 3.35 - 3.44 (m, 2H), 3.73 (dd, J = 14.1, 7.0 Hz, 1H), 7.66 (d, J = 8.3 Hz, 1H), 8.18 - 8.25 (m, 2H), 8.26 - 8.31 (m, 1H), 8.39 (d, J = 1.8 Hz, 1H), 8.66 (t, J = 5.6 Hz, 1H), 9.07 - 9.09 (m, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(te trahydropyran-2- ylmethyl)pyridine-3-carboxamide (I-T2340) [0669] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(tetrahydropyran-2-ylmethyl)pyridine-3-carb oxamide as a white solid (66.5 mg, 69%). LC-MS (Method 3): Rt = 4.33 min, m/z = 442.15 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.003 - 0.07 (m, 2H), 0.35 - 0.42 (m, 2H), 0.86 - 0.93 (m, 1H), 1.16 - 1.23 (m, 1H), 1.42 - 1.48 (m, 3H), 1.64 (br d, J = 12.84 Hz, 1H), 1.74 (s, 3H), 1.77 - 1.82 (m, 1H), 3.23 (dd, J = 13.9, 7.3 Hz, 1H), 3.32 (s, 2H), 3.34 (s, 1H), 3.42 - 3.48 (m, 1H), 3.75 (dd, J = 14.03, 7.06 Hz, 1H), 3.87 - 3.90 (m, 1H), 7.67 (d, J = 8.25 Hz, 1H), 8.2 - 8.25 (m, 2H), 8.33 (dd, J = 8.28, 2.3 Hz, 1H), 8.40 (d, J = 2.02 Hz, 1H), 8.8 (t, J = 5.8 Hz, 1H), 9.11 (dd, J = 2.29, 0.83 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(3 -fluorophenyl)methyl]pyridine-3- carboxamide (I-T2341) [0670] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[(3-fluorophenyl)methyl]pyridine-3-carboxam ide as a white solid (66.9 mg, 68%). LC-MS (Method 3): Rt = 4.73 min, m/z = 452.08 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 0.003- 0.08 (m, 2H), 0.31 - 0.42 (m, 2H), 0.85 - 0.94 (m, 1H), 1.74 (s, 3H), 3.24 (dd, J = 14.04, 7.32 Hz, 1H), 3.74 (dd, J = 13.89, 7.17 Hz, 1H), 4.54 (d, J = 5.80 Hz, 2H), 7.09 (td, J = 8.78, 2.3 Hz, 1H), 7.16 - 7.22 (m, 2H), 7.36 - 7.43 (m, 1H), 7.67 (d, J = 8.24 Hz, 1H), 8.23 - 8.26 (m, 2H), 8.37 (dd, J = 8.24, 2.14 Hz, 1H), 8.41 (d, J = 1.83 Hz, 1H), 9.16 (d, J = 1.9 Hz, 1H), 9.34 (t, J = 5.84 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (3-fluorophenyl)ethyl]pyridine-3- carboxamide (I-T2342) [0671] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[2-(3-fluorophenyl)ethyl]pyridine-3-carboxa mide as a white solid (32.2 mg, 35%). LC-MS (Method 3): Rt = 4.86 min, m/z = 466.1 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.06 (m, 2H), 0.34 - 0.40 (m, 2H), 0.86 - 0.93 (m, 1H), 1.74 (s, 3H), 2.90 (t, J = 7.2 Hz, 2H), 3.23 (dd, J = 14.0, 7.2 Hz, 1H), 3.51 - 3.58 (m, 2H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 7.02 - 7.05 (m, 1H), 7.09 - 7.12 (m, 2H), 7.32 - 7.36 (m, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.20 - 8.24 (m, 2H), 8.27 (dd, J = 8.7, 2.3 Hz, 1 H), 8.39 (d, J = 2.2 Hz, 1H), 8.83 (t, J = 5.6 Hz, 1H), 9.07 (dd, J = 2.4, 0.7 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(sp iro[2.4]heptan-7- ylmethyl)pyridine-3-carboxamide (I-T2343) [0672] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(spiro[2.4]heptan-7-ylmethyl)pyridine-3-car boxamide as a white solid (40.2 mg, 68%). LC-MS (Method 3): Rt = 5.27 min, m/z = 452.19 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.08 (m, 2H), 0.34 - 0.43 (m, 5H), 0.68 - 0.72 (m, 1H), 0.87 - 0.92 (m, 1H), 1.38 - 1.42 (m, 1H), 1.51 - 1.57 (m, 1H), 1.61 - 1.68 (m, 1H), 1.69 - 1.78 (m, 5H), 1.83 - 1.91 (m, 2H), 3.07 - 3.12 (m, 1H), 3.17 - 3.21 (m, 1H), 3.23 (dd, J = 14.1, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.20 - 8.24 (m, 2H), 8.27 - 8.31 (m, 1H), 8.39 (d, J = 2.0 Hz, 1H), 8.63 (t, J = 5.6 Hz, 1H), 9.08 (dd, J = 2.3, 0.8 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(3 ,3-difluorocyclobutyl) methyl]pyridine-3-carboxamide (I-T2345) [0673] Purification by fcc (silica gel, 0-3% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(3,3-di fluorocyclobutyl)methyl]pyridine-3- carboxamide as a white solid (50 mg, 82%). LC-MS (Method 4): Rt = 4.54 min, m/z = 448.17 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00-0.07 (m, 2H), 0.34-0.41 (m, 2H), 0.86-0.94 (m, 1H), 1.75 (s, 3H), 2.37-2.45 (m, 3H), 2.62-2.71 (m, 2H), 3.24 (dd, J = 14.1, 7.3 Hz, 1H), 3.44 (t, J = 5.9 Hz, 2H), 3.75 (dd, J = 14.1, 7.0 Hz, 1H), 7.68 (d, J = 8.2 Hz, 1H), 8.23-8.25 (m, 2H), 8.31 (dd, J = 8.3, 2.3 Hz, 1H), 8.40 (d, J = 2.0 Hz, 1H), 8.87 (t, J = 5.9 Hz, 1H), 9.11 (dd, J = 1.6, 0.6 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(2- cyclohexylethyl)pyridine-3- carboxamide (I-T2348) [0674] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(2-cyclohexylethyl)pyridine-3-carboxamide as a white solid (42.9 mg, 71%). LC- MS (Method 5b): Rt = 7.52 min, m/z = 454.23 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.07 (m, 2H), 0.34 - 0.41 (m, 2H), 0.87 - 0.96 (m, 3H), 1.11 - 1.26 (m, 3H), 1.29 - 1.37 (m, 1H), 1.43 - 1.48 (m, 2H), 1.58 - 1.64 (m, 1H), 1.64 - 1.70 (m, 2H), 1.70 - 1.77 (m, 5H), 3.24 (dd, J = 14.1, 7.3 Hz, 1H), 3.30 - 3.35 (m, 2H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.20 - 8.25 (m, 2H), 8.30 (dd, J = 8.3, 2.3 Hz, 1H), 8.40 (d, J = 2.0 Hz, 1H), 8.68 (t, J = 5.6 Hz, 1H), 9.09 (dd, J = 2.3, 0.8 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(2- thienylmethyl)pyridine-3- carboxamide (I-T2352) [0675] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(2-thienylmethyl)pyridine-3-carboxamide as a white solid (53.5 mg, 61%). LC-MS (Method 3): Rt = 4.55 min, m/z = 440.08 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.09 (m, 2H), 0.33 - 0.44 (m, 2H), 0.86 - 0.95 (m, 1H), 1.74 (s, 3H), 3.23 (dd, J = 13.9, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 4.68 (d, J = 5.9 Hz, 2H), 6.98 (dd, J = 5.1, 3.5 Hz, 1H), 7.05 - 7.07 (m, 1H), 7.41 (dd, J = 5.0, 1.2 Hz, 1H), 7.67 (d, J = 8.4 Hz, 1H), 8.22 - 8.26 (m, 2H), 8.35 (dd, J = 8.3, 2.3 Hz, 1H), 8.40 (d, J = 2.0 Hz, 1H), 9.14 (d, J = 2.2 Hz, 1H), 9.42 (t, J = 5.9 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (3-thienyl)ethyl]pyridine-3- carboxamide (I-T2353) [0676] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[2-(3-thienyl)ethyl]pyridine-3-carboxamide as a white solid (44.6 mg, 75%). LC- MS (Method 3): Rt = 4.67 min, m/z = 454.1 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.08 (m, 2H), 0.34 - 0.42 (m, 2H), 0.86 - 0.93 (m, 1H), 1.74 (s, 3H), 2.90 (t, J = 7.3 Hz, 2H), 3.24 (dd, J = 13.9, 7.3 Hz, 1H), 3.51 – 3.56 (m, 2H), 3.74 (dd, J = 14.1, 7.0 Hz, 1H), 7.05 (dd, J = 5.0, 1.3 Hz, 1H), 7.26 - 7.28 (m, 1H), 7.48 (dd, J = 4.8, 2.9 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.21 - 8.25 (m, 2H), 8.29 (dd, J = 8.3, 2.2 Hz,, 1H), 8.40 (d, J = 2.2 Hz, 1H), 8.85 (t, J = 5.5 Hz, 1H), 9.09 (dd, J = 2.2, 0.7 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(th iazol-4-ylmethyl)pyridine-3- carboxamide (I-T2354) [0677] Purification by fcc (silica gel, 0-2.5% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(thiazol -4-ylmethyl)pyridine-3-carboxamide as a white solid (60 mg, 68%). LC-MS (Method 4): Rt = 3.71 min, m/z = 441.07 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00-0.07 (m, 2H), 0.34-0.42 (m, 2H), 0.84-0.95 (m, 1H), 1.75 (s, 3H), 3.24 (dd, J = 14.1, 7.3 Hz, 1H), 3.75 (dd, J = 14.1, 7.1 Hz, 1H), 4.67 (d, J = 5.8 Hz, 2H), 7.55 (s, 1H), 7.68 (d, J = 8.2 Hz, 1H), 8.24 (s, 1H), 8.26 (s, 1H), 8.38 (dd, J = 8.3, 2.2 Hz, 1H), 8.42 (d, J = 1.7 Hz, 1H), 9.09 (d, J = 1.6 Hz, 1H), 9.17 (d, J = 2.1Hz, 1H), 9.36 (t, J = 5.5 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(th iazol-5-ylmethyl)pyridine-3- carboxamide (I-T2355) [0678] Purification by fcc (silica gel, 0-3% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(thiazol -5-ylmethyl)pyridine-3-carboxamide as a white solid (41 mg, 69%). LC-MS (Method 4): Rt = 3.82 min, m/z = 441.03 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00-0.07 (m, 2H), 0.34-0.42 (m, 2H), 0.86-0.93 (m, 1H), 1.74 (s, 3H), 3.24 (dd, J = 14.1, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.0 Hz, 1H), 4.74 (d, J = 5.8 Hz, 2H), 7.67 (d, J = 8.3 Hz, 1H), 7.87 (d, J = 0.7 Hz, 1H), 8.22-8.26 (m, 2H), 8.34 (dd, J = 8.3, 2.3Hz, 1H), 8.41 (d, J = 2.0 Hz, 1H), 9.00 (d, J = 0.7 Hz, 1H) 9.13 (dd, J = 2.2, 0.6 Hz, 1H), 9.46 (t, J = 5.7 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(th iazol-2-ylmethyl)pyridine-3- carboxamide (I-T2356) [0679] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(thiazol -2-ylmethyl)pyridine-3-carboxamide as a white solid (29 mg, 33%). LC-MS (Method 4): Rt = 3.95 min, m/z = 441.03 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00-0.07 (m, 2H), 0.35-0.42 (m, 2H), 0.86-0.94 (m, 1H), 1.75 (s, 3H), 3.24 (dd, J = 14.0, 7.3 Hz, 1H), 3.75 (dd, J = 14.0, 7.1 Hz, 1H), 4.81 (d, J = 5.9 Hz, 2H), 7.66 (d, J = 3.2 Hz, 1H), 7.68 (d, J = 8.3 Hz, 1H), 7.76 (d, J = 3.2 Hz, 1H), 8.25 (dd, J = 8.3, 2.0 Hz, 1H), 8.27 (d, J = 8.4 Hz, 1H), 8.38 (dd, J = 8.3, 2.3 Hz, 1H), 8.42 (d, J = 2.0 Hz, 1H), 9.17 (d, J = 2.2 Hz, 1H), 9.68 (t, J = 5.9 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(2- thiazol-5-ylethyl)pyridine-3- carboxamide (I-T2360) [0680] Purification by fcc (silica gel, 0-3% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(2-thiaz ol-5-ylethyl)pyridine-3-carboxamide as a white solid (46 mg, 51%). LC-MS (Method 4): Rt = 3.87 min, m/z = 455.08 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): -0.02-0.06 (m, 2H), 0.33-0.41 (m, 2H), 0.84-0.94 (m, 1H), 1.73 (s, 3H), 3.15 (t, J = 6.7 Hz, 2H), 3.23 (dd, J = 14.0, 7.2 Hz, 1H), 3.55 (q, J = 5.9 Hz, 2H), 3.74 (dd, J = 13.9, 7.1 Hz, 1H), 7.66 (d, J = 8.2 Hz, 1H), 7.72 (d, J = 0.7 Hz, 1H), 8.21-8.24 (m, 2H), 8.27-8.30 (m, 1H), 8.39 (d, J = 2.0 Hz, 1H), 8.91 (t, J = 5.7 Hz, 1H), 8.94 (d, J = 0.7 Hz, 1H), 9.08 (dd, J = 2.1, 0.5 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(6 -cyano-3-pyridyl)methyl] pyridine-3-carboxamide (I-T2371) [0681] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(6-cyan o-3-pyridyl)methyl]pyridine-3- carboxamide as a white solid (36 mg, 59%). LC-MS (Method 5b): Rt = 5.85 min, m/z = 460.12 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00-0.07 (m, 2H), 0.35-0.42 (m, 2H), 0.85-0.95 (m, 1H), 1.75 (s, 3H), 3.24 (dd, J = 14.0, 7.3 Hz, 1H), 3.75 (dd, J = 14.0, 7.1 Hz, 1H), 4.64 (d, J = 5.97 Hz, 2H), 7.68 (d, J = 8.3 Hz, 1H), 7.99-8.04 (m, 2H), 8.24 (dd, J = 8.2, 2.1 Hz, 1H), 8.26 (d, J = 8.1 Hz, 1H), 8.36 (dd, J = 8.3, 2.3 Hz, 1H), 8.41 (d, J = 2.0 Hz, 1H), 8.78 (s, 1H) 9.16 (d, J = 2.2 Hz, 1H), 9.44 (t, J = 5.6 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(6 -ethoxy-3-pyridyl)methyl] pyridine-3-carboxamide (I-T2373) [0682] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[(6-ethoxy-3-pyridyl)methyl]pyridine-3-carb oxamide as a white solid (27.8 mg, 44%). LC-MS (Method 3): Rt = 4.43 min, m/z = 479.12 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): 0.01 - 0.06 (m, 2H), 0.35 - 0.41 (m, 2H), 0.87 - 0.93 (m, 1H), 1.30 (t, J = 7.1 Hz, 3H), 1.74 (s, 3H), 3.24 (dd, J = 14.0, 7.2 Hz, 1H), 3.74 (dd, J = 13.9, 7.0 Hz, 1H), 4.28 (q, J = 7.2 Hz, 2H), 4.45 (d, J = 5.7 Hz, 2H), 6.78 (d, J = 8.6 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 7.69 (dd, J = 8.5, 2.5 Hz, 1H), 8.14 (d, J = 2.0 Hz, 1H), 8.22 - 8.25 (m, 2H), 8.34 (dd, J = 8.3, 2.3 Hz, 1H), 8.40 (d, J = 2.0 Hz, 1H), 9.13 - 9.14 (m, 1H), 9.26 (t, J = 5.7 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[(6 -bromo-3-pyridyl)methyl] pyridine-3-carboxamide (I-T2370) [0683] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-[(6-bromo-3-pyridyl)methyl]pyridine-3-carbo xamide as a white solid (53 mg, 78%). LC-MS (Method 5b): Rt = 6.09 min, m/z = 515.06 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.07 (m, 2H), 0.34 - 0.41 (m, 2H), 0.85 - 0.94 (m, 1H), 1.74 (s, 3H), 3.24 (dd, J = 14.0, 7.2 Hz, 1 H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 4.52 (d, J = 5.7 Hz, 2H), 7.64 (d, J = 8.3 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 7.74 (dd, J = 8.2, 2.5 Hz, 1H), 8.22 - 8.26 (m, 2H), 8.35 (dd, J = 8.3, 2.3 Hz, 1H), 8.41 (d, J = 2.0 Hz, 2H), 9.13 - 9.15 (m, 1H), 9.33 (t, J = 5.7 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[3- (1-methylpyrazol-4- yl)propyl]pyridine-3-carboxamide (I-T2366) [0684] Purification by reverse phase flash chromatography (0.1% aq formic acid to MeCN) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[3- (1-methylpyrazol-4- yl)propyl]pyridine-3-carboxamide as a white solid (53 mg, 87%). LC-MS (Method 3): Rt = 3.88 min, m/z = 465.98 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.01 - 0.07 (m, 2H), 0.34 - 0.42 (m, 2H), 0.86 - 0.94 (m, 1H), 1.74 (s, 3 H), 1.78 (quin, J = 7.3 Hz, 2H), 2.46 (t, J = 7.5 Hz, 2H), 3.29 – 3.34 (solvent signal including m, 2H), 3.24 (dd, J = 14.0, 7.2 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 3.77 (s, 3H), 7.27 (s, 1H), 7.49 (s, 1H), 7.67 (d, J = 8.4 Hz, 1H), 8.21 - 8.24 (m, 2H), 8.31 (dd, J = 8.4, 2.2 Hz, 1H), 8.40 (d, J = 2.0 Hz, 1H), 8.73 (t, J = 5.5 Hz, 1H), 9.10 (dd, J = 2.3, 0.8 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-(ox azol-2-ylmethyl)pyridine-3- carboxamide (I-T2364) [0685] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- chloro-phenyl]-N-(oxazol-2-ylmethyl)pyridine-3-carboxamide as a white solid (24.6 mg, 44%). LC- MS (Method 5b): Rt = 5.23 min, m/z = 425.10 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.07 (m, 2H), 0.35 - 0.41 (m, 2H), 0.86 - 0.93 (m, 1H), 1.74 (s, 3H), 3.24 (dd, J = 13.9, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 4.64 (d, J = 5.9 Hz, 2H), 7.18 (d, J = 0.7 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.07 (d, J = 0.7 Hz, 1H), 8.23 - 8.27 (m, 2H), 8.36 (dd, J = 8.3, 2.3 Hz, 1H), 8.41 (d, J = 2.1 Hz, 1H), 9.14 - 9.16 (m, 1H), 9.45 (t, J = 5.8 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[[6 -(difluoromethyl)-3-pyridyl] methyl]pyridine-3-carboxamide (I-T2397) [0686] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropyl methyl)amino]-3-chloro-phenyl]-N-[[6-(difluoromethyl)-3-pyri dyl]methyl]pyridine-3-carboxamide as a white solid (42.5 mg, 65%). LC-MS (Method 5b): Rt = 5.90 min, m/z = 485.15 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.01 - 0.07 (m, 2H), 0.34 - 0.40 (m, 2H), 0.85 - 0.94 (m, 1H), 1.74 (s, 3H), 3.23 (dd, J = 14.1, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 4.61 (d, J = 5.7 Hz, 2H), 6.95 (t, J = 54.8 Hz, 1H), 7.66 - 7.70 (m, 2H), 7.97 (dd, J = 8.1, 2.0 Hz, 1H), 8.23 - 8.27 (m, 2H), 8.36 (dd, J = 8.3, 2.3 Hz, 1H), 8.41 (d, J = 2.0 Hz, 1H), 8.70 (d, J = 1.5 Hz, 1H), 9.16 (dd, J = 2.3, 0.8 Hz, 1H), 9.41 (t, J = 5.9 Hz, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (4-methylthiazol-5- yl)ethyl]pyridine-3-carboxamide (I-T2426) [0687] Purification by fcc (silica gel, 0-10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropyl methyl)amino]-3-chloro-phenyl]-N-[2-(4-methylthiazol-5-yl)et hyl]pyridine-3-carboxamide as a white solid (37.1 mg, 61%). LC-MS (Method 1): Rt = 1.63 min, m/z = 469.11 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): 0.00 - 0.06 (m, 2H), 0.35 - 0.41 (m, 2H), 0.85 - 0.94 (m, 1H), 1.74 (s, 3H), 2.33 (s, 3H), 3.06 (t, J = 7.0 Hz, 2H), 3.24 (dd, J = 14.0, 7.2 Hz, 1H), 3.47 - 3.52 (m, 2H), 3.74 (dd, J = 14.1, 7.0 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.21 - 8.26 (m, 2H), 8.28 - 8.30 (m, 1H), 8.40 (d, J = 2.2 Hz, 1H), 8.83 (s, 1H), 8.92 (t, J = 5.3 Hz, 1H), 9.08 - 9.10 (m, 1H) Examples I-T2303 and I-T2304 [0688] For examples I-T2303 and I-T2304, 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2- (trifluoromethyl)aniline was first prepared as follows and then Scheme 1 (steps 2a/3a) was followed to yield the final compounds. [0689] A suspension of 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (1 g, 4.56 mmol), 1- (trifluoromethyl)-1lambda3,2-benziodoxol-3-one (40%, 1.80 g, 2.28 mmol), nickel(II) hydroxide (21.2 mg, 0.228 mmol) and K 2 CO 3 (946 mg, 6.85 mmol) in DMSO (9 mL) was purged with argon and heated at 35 °C overnight. The RM was filtered through celite, and the filtrate washed with EtOAc. Water was added and the layers were separated. The aqueous layer was extracted with EtOAc. The organic layers were combined, dried, and concentrated in vacuo to give the crude product. Purification by fcc (silica gel, 0-40% EtOAc in cyclohexane) afforded 4-(4,4,5,5-tetramethyl- 1,3,2-dioxaborolan-2-yl)-2-(trifluoromethyl)aniline as a light-yellow solid (295 mg, 44%). LC-MS (Method 1): Rt = 2.36 min, m/z = 288.04 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.24 (s, 12H), 6.00 (s, 2H), 6.78 (d, J = 8.3 Hz, 1H), 7.50 (d, J = 8.3 Hz, 1H), 7.57 (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-(trifluoromethyl)phe nyl]-N-[2-(3-pyridyl)ethyl] pyridine-3-carboxamide (I-T2303) [0690] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-(trifluoromethyl)phenyl]- N-[2-(3-pyridyl)ethyl]pyridine-3- carboxamide as a white solid (28.3 mg, 44%). LC MS/UV (Method 4): Rt = 4.04 min, m/z = 482.98 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ -0.04-0.03 (m, 1H), 0.10-0.18 (m, 1H), 0.36-0.46 (m, 2H), 0.94-1.04 (m, 1H), 1.71 (s, 3H), 2.69 (dd, J = 14.0, 7.9 Hz, 1H), 2.90 (t, J = 7.02 Hz, 2H), 3.57 (q, J = 6.7 Hz, 2H), 4.21 (dd, J = 14.2, 6.3 Hz, 1H), 7.32 (dd, J = 7.7, 4.7 Hz, 1H), 7.69 (d, J = 7.9 Hz, 1H), 7.78 (d, J = 8.2 Hz, 1H), 8.29 (s, 2H), 8.42 (d, J = 4.6 Hz, 1H), 8.48 (s, 1H), 8.55 (d, J = 8.2 Hz, 1H), 8.60 (s, 1H), 8.81-8.90 (m, 1H), 9.08 (s, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-(trifluoromethyl)phe nyl]-N-[3-(1H-pyrazol-4-yl) propyl]pyridine-3-carboxamide (I-T2304) [0691] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-(trifluoromethyl)phenyl]- N-[3-(1H-pyrazol-4- yl)propyl]pyridine-3-carboxamide as a white solid (48.6 mg, 54%). LC-MS (Method 4): Rt = 3.94 min, m/z = 485.97 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ -0.03-0.02 (m, 1H), 0.12-0.17 (m, 1H), 0.36-0.47 (m, 2H), 0.95-1.03 (m, 1H), 1.71 (s, 3H), 1.76-1.82 (m, 2H), 2.48-2.53 (m, 2H), 2.66- 2.72 (m, 1H), 3.31-3.35 (m, 2H), 4.17-4.23 (m, 1H), 7.35 (bs, 1H), 7.54 (bs, 1H), 7.78 (d, J = 8.3 Hz, 1H), 8.29 (d, J = 8.2 Hz, 1H), 8.35 (dd, J = 8.2 Hz, 2.1 Hz, 1H), 8.56 (d, J = 3.5 Hz, 1H), 8.60 (bs, 1H), 8.76 (t, J = 5.3 Hz, 1H), 9.13 (d, J = 1.3 Hz, 1H), 12.52 (bs, 1H). Scheme 1 : Procedures 2b/3b Step 2b: Methyl 6-[3-chloro-4-(2,2-difluoroethylamino)phenyl]pyridine-3-carb oxylate [0692] Methyl 6-(4-amino-3-chloro-phenyl)pyridine-3-carboxylate (12.5 g, 47.1 mmol) was suspended in dry DCM (36 mL) and cooled to 10 °C under N2 atmosphere. To the suspension, was added 2,2,2-trifluoroacetic acid (72.1 mL, 942 mmol). The reaction mixture turned dark red.1- Ethoxy-2,2-difluoro-ethanol (6.60 mL, 54.2 mmol) was added, and the resulting mixture was stirred at RT for 2h, then cooled down to 0 °C and NaBH(OAc) 3 (15.0 g, 70.7 mmol) was added in portions over 40 min. After 2h of stirring, the RM was cooled to 5 °C, and neutralized with 40% NaOH to pH = 8. Some DCM was removed under reduced pressure, after filtration, the resulting solid was dried under reduced pressure for 20 min. The solid was triturated in MeOH (70 mL) at 45 °C for 10 min, cooled to RT and filtered. It was washed with MeOH, dried under reduced pressure for 15 min and in the vacuum oven at 45 °C for 16h to yield methyl 6-[3-chloro-4-(2,2-difluoroethylamino)phenyl] pyridine-3-carboxylate (14.7 g, 95.5%) as a gray solid. LC-MS (Method 1): Rt = 2.14 min, m/z = 327.08 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 3.65-3.79 (m, 2H), 3.88 (s, 3H), 6.02-6.31 (m, 2H), 7.015 (d, J = 8.75 Hz, 1H), 8.00 (dd, J = 8.74, 2.05 Hz, 1H), 8.03 (d, J = 8.56 Hz, 1H), 8.15 (d, J = 1.99 Hz, 1H), 8.24 (dd, J = 8.5, 2.2 Hz, 1H), 9.06 (d, J = 1.65 Hz, 1H). Step 3b: Methyl 6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]pyridi ne-3-carboxylate [0693] A solution of methyl 6-[3-chloro-4-(2,2-difluoroethylamino)phenyl]pyridine-3-carb oxylate (33.0 g, 101 mmol) in acetic anhydride (319 mL) was heated at 112 °C for 21h and then at 130 °C for 2h. The RM was cooled to RT and poured into a mixture of water (1000 mL) and DCM (500 mL). The pH of the mixture was adjusted to pH = 9 with 40% NaOH. The layers were separated, and the H 2 O layer was extracted with DCM (2 x 500 mL). The organic layers were gathered and concentrated to afford the crude product (43.4 g) as a gray solid. It was triturated in acetone (100 mL) for 20 min in an ultrasonic bath. To the suspension, H 2 O (200 mL) was added, stirring was continued for 30 min. After filtration, the solid was dried under reduced pressure for 30 min, then in a vacuum oven at 45 °C for 19h to yield methyl 6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]pyridi ne-3-carboxylate (34.0 g, 90%) as an off white solid. LC-MS (Method 1): Rt = 2.00 min, m/z = 369.16 [M+H] + . 1 H- NMR (500 MHz, DMSO-d 6 ): δ 1.79 (s, 3H), 3.66-3.78 (m, 1H), 3.91 (s, 3H), 4.21-4.34 (m, 1H), 6.2 (tt, J = 55.59, 4.2 Hz, 1H), 7.7 (d, J = 8.15 Hz, 1H), 8.24-8.3 (m, 2H), 8.4 (dd, J = 8.21, 2.13 Hz, 1H), 8.43 (d, J = 1.98 Hz, 1H), 9.18 (d, J = 1.93, 1H). Following Scheme 1 : Procedures 2b/3b 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-4-yl)propyl]pyridine- 3-carboxamide (I-T2108) [0694] Purification by fcc (silica gel, 10% MeOH in DCM), followed by prep HPLC (Method 7) afforded 6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-4- yl)propyl]pyridine-3-carboxamide (9.62 mg, 15%) as a white solid. LC-MS (Method 5): Rt = 4.93 min; m/z = 462.15 [M+H] + . 1 H-NMR (600 MHz, DMSO-d 6 ): δ 1.83-1.76 (m, 5H), 2.52-2.54 (m, 2H), 3.3 (m, 2H), 3.72 (qd, J = 14.3, 4.7 Hz, 1H), 4.35-4.21 (m, 1H), 6.21 (tt, J = 55.8, 3.8 Hz, 1H), 7.45 (bs, 2H), 7.69 (d, J = 8.3 Hz, 1H), 8.22 (dd, J = 8.3, 0.8 Hz, 1H), 8.25 (dd, J = 8.3, 2.1 Hz, 1H), 8.32 (dd, J = 8.3, 2.3 Hz, 1H), 8.41 (d, J = 2.1 Hz, 1H), 8.75 (t, J = 5.6 Hz, 1H), 9.11- 9.10 (m, 1H), 12.50 (bs, 1H). 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-[2- (1H-pyrazol-4-yl)ethyl]pyridine-3- carboxamide (I-T2114) [0695] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(2,2-difluoroethyl)amino]-3- chloro-phenyl]-N-[2-(1H-pyrazol-4-yl)ethyl]pyridine-3-carbox amide as a white solid (36.7 mg, 48%). LC-MS (Method 5): Rt = 4.92 min, m/z = 448.13 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 1.79 (s, 3H), 2.71 (t, J = 8.2 Hz, 2H), 3.45 (m,2H), 3.6-3.81 (m, 1H), 4.16-4.37 (m, 1H), 6.21 (tt, J = 55.7 Hz, 4.05 Hz, 1H), 7.28-7.62 (br m, 2H) 7.67 (d, J = 8.59 Hz, 1H) 8.16 - 8.26 (m, 2H) 8.29 (dd, J = 8.4 Hz, 2.2 Hz, 1H), 8.4 (d, J = 1.94 Hz, 1H), 8.82 (t, J = 5.58 Hz, 1H), 9.08 (d, J = 1.65 Hz, 1H) 12.57 (br s, 1H). 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-[2- (3-pyridyl)ethyl]pyridine-3- carboxamide (I-T2205) [0696] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(2,2-difluoroethyl)amino]-3- chloro-phenyl]-N-[2-(3-pyridyl)ethyl]pyridine-3-carboxamide as a white solid (39 mg, 60%). LC-MS (Method 5): Rt = 4.28, m/z = 459.13 [M+H] + . 1 H-NMR (300 MHz, DMSO-d 6 ): δ 1.78 (s, 3H), 2.89 (t, J = 6.97, 2H), 3.50-3.60 (m, 2H), 3.61-3.80 (m, 1H), 4.15-4.37 (m, 1H), 6.2 (tt, J =55.4 Hz, 4 Hz, 1H), 7.26-7.37 (m, 1H), 7.67 (m, 2H), 8.15 - 8.30 (m, 3H), 8.36-8.43 (m, 2H), 8.47 (d, J = 1.8 Hz, 1H), 8.84 (t, J = 5.6 Hz, 1H), 9.04 (d, J = 1.4 Hz, 1H). 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-[3- (1H-pyrazol-3-yl)propyl]pyridine- 3-carboxamide (I-T2287) [0697] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(2,2-difluoroethyl)amino]-3- chloro-phenyl]-N-[3-(1H-pyrazol-3-yl)propyl]pyridine-3-carbo xamide as a white solid (10 mg, 14%). LC-MS (Method 5): Rt = 5.08, m/z = 462.14 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.79 (s, 3H), 1.80 – 1.92 (m, 2H), 2.56-2.69 (br m, 2H), 3.35 (m, 2H), 3.60 – 3.81 (m, 1H), 4.15 - 4.37 (m, 1H), 6.03 - 6.09 (bs, 1H), 6.2 (tt, J = 55.5 Hz, 4.04 Hz, 1H), 7.32 (bs, 0.5H), 7.58 (bs, 0.5H), 7.68 (d, J = 8.34 Hz, 1H), 8.16 – 8.26 (m, 2H), 8.31 (dd, J = 8.34, 2.16 Hz, 1H), 8.40 (d, J = 1.9 Hz, 1H), 8.76 (t, J = 5.4 Hz, 1H), 9.09 (d, J = 1.7 Hz, 1H), 12.41 - 12.51 (m, 1H). 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-(3- imidazol-1-ylpropyl)pyridine-3- carboxamide (I-T2288) [0698] Purification by prep HPLC (Method 6) afforded 6-[4-[acetyl(2,2-difluoroethyl)amino]-3- chloro-phenyl]-N-[3-(1H-pyrazol-3-yl)propyl]pyridine-3-carbo xamide as a white solid (45.6 mg, 70%). LC-MS (Method 5): Rt = 4.24, m/z = 462.14 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.78 (s, 3H), 1.98 (m, 2H), 3.27 (m, 2H) 3.62-3.80 (m, 1H), 4.04 (t, J = 6.9 Hz, 2H), 4.17-4.37 (m, 1H), 6.2 (tt, J = 55.4 Hz, 4.2 Hz, 1H), 6.88 (s, 1H), 7.21 (s, 1H), 7.62-7.72 (m, 2H), 8.17 - 8.26 (m, 2H), 8.30 (dd, J =8.34 Hz, 2.18 Hz, 1H), 8.4 (d, J = 2.08 Hz, 1H), 8.76 (t, J = 5.47 Hz, 1H), 9.09 (d, J = 1.72 Hz, 1H). 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-imi dazo[1,2-a]pyrimidin-6yl-pyridine- 3-carboxamide (I-T2201) [0699] Purification by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4-[acetyl(2,2- difluoroethyl)amino]-3-chloro-phenyl]-N-imidazo[1,2-a]pyrimi din-6yl-pyridine-3-carboxamide as a white solid (15 mg, 22%). LC-MS (Method 3): Rt = 3.04, m/z = 470.75 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 1.81 (s, 3H), 3.67 – 3.79 (m, 1H,), 4.23 - 4.35 (m, 1H), 6.22 (tt, J = 55.5, 4.07 Hz, 1H), 7.69 – 7.74 (m, 2H), 8.05 (s, 1H), 8.30 (d, J = 8.10 Hz, 1H), 8.35 (d, J = 8.10 Hz, 1H), 8.44-8.47 (m, 1H), 8.48 - 8.52 (m, 1H), 8.70 - 8.74 (m, 1H), 9.27 (m, 1H), 9.66-9.70 (m, 1H), 10.88 (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (4-pyridyl)ethyl]pyridine-3- carboxamide (I-T2308) [0700] Purification by fcc (silica gel, 0-10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropyl methyl)amino]-3-chloro-phenyl]-N-[2-(4-pyridyl)ethyl]pyridin e-3-carboxamide as a white solid (37.3 mg, 57%). LC-MS (Method 4): Rt = 3.81 min, m/z = 448.96 [M+H] + . 1 H NMR (500 MHz, DMSO- d 6 ): δ -0.01-0.07 (m, 2H), 0.34-0.41 (m, 2H), 0.83-0.93 (m, 1H), 1.74 (s, 3H), 2.90 (t, J = 7.2 Hz, 2H), 3.21-3.26 (m, 1H), 3.50-3.55 (m, 2H), 3.71-3.77 (m, 1H), 7.29 (d, J = 5.9 Hz, 2H), 7.67 (d, J = 8.2 Hz, 1H), 8.20-8.24 (m, 2H), 8.27 (dd, J = 8.2 Hz, J = 2.1 Hz, 1H), 8.39 (d, J = 2.0 Hz, 1H), 8.45- 8.49 (m, 2H), 8.84 (t, J = 5.5 Hz, 1H), 9.06 (d, J = 1.9 Hz, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2- (6-methyl-3-pyridyl)ethyl] pyridine-3-carboxamide (I-T2309) [0701] Following Scheme 1: purification by fcc (silica gel, 0-10% MeOH in DCM) afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-N-[2-(6-me thyl-3-pyridyl)ethyl]pyridine-3- carboxamide as a white solid (32.4 mg, 48%). LC-MS (Method 4): Rt = 4.01 min, m/z = 462.91 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ -0.01-0.07 (m, 2H), 0.34-0.41 (m, 2H), 0.84-0.93 (m, 1H), 1.74 (s, 3H), 2.42 (s, 3H), 2.85 (t, J = 7.2 Hz, 2H), 3.21-3.26 (m, 1H), 3.55-3.60 (m, 2H), 3.71- 3.76 (m, 1H), 7.18 (d, J = 7.9 Hz, 1H), 7.56 (dd, J = 7.9 Hz, J = 2.1 Hz, 1H), 7.67 (d, J = 8.3 Hz, 1H), 8.20-8.24 (m, 2H), 8.27 (dd, J = 8.2 Hz, J = 2.0 Hz, 1H), 8.32 (d, J = 1.6 Hz, 1H), 8.39 (d, J = 1.9 Hz, 1H), 8.82 (t, J = 5.5 Hz, 1H), 9.06 (d, J = 1.8 Hz, 1H). 6-[4-[Acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-ben zyl-pyridine-3-carboxamide (I- T2284) [0702] Following Scheme 1: purification by fcc (silica gel, 0-3 % MeOH in DCM) afforded 6-[4- [acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-N-benzyl-p yridine-3-carboxamide as a white foam (66 mg, 73%). LC-MS (Method 3): Rt = 4.49 min, m/z = 443.87 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.78 (s, 3H), 3.60 - 3.80 (m, 1H), 4.16 - 4.38 (m, 1H), 4.52 (d, J = 5.89 Hz, 2H), 6.2 (tt, J = 55.3, 4.13 Hz, 1H), 7.19 - 7.29 (m 1H), 7.30 – 7.39 (m, 4H), 7.68 (d, J = 8.29 Hz, 1H), 8.20 – 8.28 (m, 2H), 8.36 (dd, J = 8.37, 2.27 Hz, 1H), 8.41 (d, J = 1.92 Hz, 1H), 9.15 (d, J = 1.9 Hz, 1H), 9.31 (t, J = 5.87 Hz, 1H). Scheme 2 Step 1: 2-Chloro-4-(5-nitro-2-pyridyl)aniline [0703] To a mixture of 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (5.0 g, 19.7 mmol), 2- bromo-5-nitro-pyridine (4.0 g, 19.7 mmol) and K 3 PO 4 (12.6 g, 59.2 mmol), was added a mixture of 1,4 dioxane (100 mL) and water (10 mL). The reaction mixture was degassed with nitrogen for 10 min and heated to 70 °C when Pd(Ph 3 P) 4 (1.04 g, 0.90 mmol) was added. The reaction mixture was stirred at 70 °C for 3h. After cooling to RT, EtOAc and sat. NaHCO 3 were added. The aqueous layer was separated and extracted with EtOAc. The organic layers were combined together, washed with brine and concentrated in vacuo. The resulting residue was triturated from ACN, methanol and then DCE to afford 2-chloro-4-(5-nitro-2-pyridyl)aniline as a brown solid (1.6 g, 30%). LC-MS (Method 2): Rt = 1.86 min; m/z = 250.04 [M+H] + . [0704] Alternatively, 2-chloro-4-(5-nitro-2-pyridyl)aniline can be synthesized following Scheme 1 – Step 1, followed by trituration with ACN, methanol and then DCE. Step 2: 2-Chloro-N-(cyclopropylmethyl)-4-(5-nitro-2-pyridyl)aniline [0705] To a suspension of 2-chloro-4-(5-nitro-2-pyridyl)aniline (1.4 g, 90%, 5.0 mmol) in 1,2- dichloroethane (38 mL), were added cyclopropanecarbaldehyde (0.45 mL, 6.1 mmol) and CH 3 CO 2 H (66 μL,1.2 mmol) under N 2 atmosphere. Molecular sieves 4Å (700 mg) were added and the RM was stirred at RT for 2h. It was cooled to 0 °C and NaBH(CH 3 COO) 3 (1.60 g, 7.6 mmol) was added in small portions over 30 min. The RM was then left to stir at RT overnight. The resulting solution was diluted with DCM and washed with sat. NaHCO 3 , dried and concentrated in vacuo. Purification by fcc (silica gel, 0-10% EtOAc in cyclohexane) afforded 2-chloro-N-(cyclopropylmethyl)-4-(5-nitro-2- pyridyl)aniline (950 mg, 62%). LC-MS (Method 2): Rt = 2.62 min; m/z = 304.06 [M+H] + .1H NMR (500 MHz, DMSO-d 6 ): δ 0.26 - 0.30 (m, 2H), 0.45 - 0.50 (m, 2H), 1.10 - 1.18 (m, 1H), 3.14 (t, J = 6.26 Hz, 2H), 6.07 (t, J = 5.80 Hz, 1H), 6.91 (d, J = 8.85 Hz, 1H), 8.06 (dd, J = 8.85, 2.14 Hz, 1H), 8.12 (d, J = 8.85 Hz, 1H), 8.19 (d, J = 2.14 Hz, 1H), 8.51 (dd, J = 8.85, 2.75 Hz, 1H), 9.32 (d, J = 2.44 Hz, 1H). [0706] Alternatively, 2-chloro-N-(cyclopropylmethyl)-4-(5-nitro-2-pyridyl)aniline can be synthesized following Scheme 1 – Step 2a, followed by purification by fcc (silica gel, 0-10% EtOAc in cyclohexane). Step 3: N-[2-chloro-4-(5-nitro-2-pyridyl)phenyl]-N-(cyclopropylmethy l)acetamide [0707] To stirred solution of 2-chloro-N-(cyclopropylmethyl)-4-(5-nitro-2-pyridyl)aniline (950 mg, 3.1 mmol) in dry DCM (20 mL) at 0 °C, was added acetyl chloride (890 µL, 13 mmol). The resulting mixture was stirred at RT overnight. Water was added and the pH adjusted to 8 with 10% NaOH. The layers were separated, the organic layer was washed with brine, dried and concentrated in vacuo to afford crude N-[2-chloro-4-(5-nitro-2-pyridyl)phenyl]-N-(cyclopropylmethy l)acetamide (967 mg, 89%) which was used as such in the next step. LC-MS (Method 2): Rt = 2.13 min; m/z = 346.09 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 0.01-0.10 (m, 2H), 0.32 - 0.41 (m, 2H), 0.82 - 0.94 (m, 1H), 1.74 (s, 3H), 3.20 - 3.29 (m, 1H), 3.68 - 3.78 (m, 1H), 7.72 (d, J = 8.36 Hz, 1H), 8.29 (dd, J = 8.27, 1.83 Hz, 1H), 8.38 (d, J = 8.88 Hz, 1H), 8.46 (d, J = 1.92 Hz, 1H), 8.70 (dd, J = 8.71, 2.61 Hz, 1H), 9.46 (d, J = 2.61 Hz, 1H). [0708] Alternatively, N-[2-chloro-4-(5-nitro-2-pyridyl)phenyl]-N-(cyclopropylmethy l) acetamide can be synthesized following Scheme 1, Step 3a starting from 2-chloro-N-(cyclopropyl methyl)-4-(5- nitro-2-pyridyl)aniline (950 mg, 3.1 mmol). Step 4: N-[4-(5-amino-2-pyridyl)-2-chloro-phenyl]-N-(cyclopropylmeth yl)acetamide [0709] A suspension N-[2-chloro-4-(5-nitro-2-pyridyl)phenyl]-N-(cyclopropylmethy l)acetamide (915 mg, 2.64 mmol) and ammonium chloride (566 mg, 11 mmol) in EtOH (37 mL) and water (18 mL) was heated to 80 °C, Fe (1.77 g, 32 mmol) was then added. The RM was stirred at 80 °C for 45 min. It was diluted with DCM, filtered and the filtrate concentrated in vacuo. The residue was diluted with water and extracted with DCM. The organic layers were combined together, washed with water, brine and concentrated in vacuo to afford crude N-[4-(5-amino-2-pyridyl)-2-chloro-phenyl]-N- (cyclopropylmethyl)acetamide (845 mg) as a light brown solid which was used as such in the next step. LC-MS (Method 2): Rt = 1.57 min; m/z = 316.08 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 0.01 - 0.06 (m, 2H), 0.34 - 0.41 (m, 2H), 0.84 - 0.92 (m, 1H), 1.72 (s, 3H), 3.18-3.23 (m, 1H), 3.69 - 3.75 (m, 1H), 5.64 (s, 2H) , 7.00 (dd, J = 8.54, 2.75 Hz, 1H), 7.50 (d, J = 8.24 Hz, 1H), 7.75 (d, J = 8.54 Hz, 1H), 7.96 (dd, J = 8.39, 1.98 Hz, 1H), 8.03 (d, J = 2.75 Hz, 1H), 8.14 (d, J = 1.83 Hz, 1H). Step 5: N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyloxy)- acetamide (I-T2119) [0710] To a solution of 2-(3-pyridyloxy)acetic acid hydrochloride (50.0 mg, 0.26 mmol) in dry DCM (2 mL), were added HATU (121 mg, 0.32 mmol) and DIPEA (91.9 µL, 0.53 mmol). The reaction mixture was stirred at RT for 30 min when N-[4-(5-amino-2-pyridyl)-2-chloro-phenyl]-N- (cyclopropylmethyl)acetamide (99.9 mg, 0.32 mmol) was added. Stirring was continued at RT overnight. The reaction was quenched with sat. NaHCO 3 , the layers were separated. The organic layer was washed with brine, dried and concentrated in vacuo. Purification by fcc (silica gel, 10% MeOH in DCM) afforded N-[6-[4-[acetyl(cyclopropylmethyl) amino]-3-chloro-phenyl]-3-pyridyl]-2-(3- pyridyloxy)acetamide (39.21 mg, 32%) as a white solid. LC-MS (Method 4): Rt = 3.97 min; m/z = 451.18 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01-0.07 (m, 2H), 0.33-0.41 (m, 2H), 0.84-0.94 (m, 1H), 1.73 (s, 3H), 3.19-3.24 (m, 1H), 3.71- 3.76 (m, 1H), 4.88 (s, 2H), 7.35- 7.39 (m, 1H), 7.44- 7.48 (m, 1H), 7.61 (d, J = 8.24 Hz, 1H), 8.08-8.14 (m, 2H), 8.21-8.24 (m, 2H), 8.30 (d, J = 1.83 Hz, 1H), 8.40 (d, J = 3.05 Hz, 1H), 8.91 (d, J = 2.36 Hz, 1H), 10.52 (s, 1H). [0711] Alternatively, N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3- pyridyloxy)acetamide can be synthesized following Scheme 1 – Step 5, starting from 2-(3- pyridyloxy)acetic acid hydrochloride (50.0 mg, 0.26 mmol), and then purification by fcc (silica gel, 10% MeOH in DCM). Following Scheme 2: (E)-N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl ]-3-pyridyl]-3-(3-pyridyl)prop-2- enamide (I-T2124) [0712] Purification by fcc (silica gel, 10% MeOH in DCM) afforded (E)-N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -3-(3-pyridyl)prop-2-enamide as a light-yellow solid (69.1 mg, 45%). LC-MS (Method 4): Rt = 4.17 min; m/z = 447.12 [M+H] + . 1 H- NMR (500 MHz, DMSO-d 6 ): δ 0.01- 0.07 (m, 2H), 0.33- 0.41 (m, 2H), 0.85-0.94 (m, 1H), 1.73 (s, 3H), 3.18-3.24 (m, 1H), 3.71-3.77 (m, 1H), 6.95 (d, J = 15.92 Hz, 1H), 7.50 (dd, J = 7.88, 4.76 Hz, 1H), 7.61 (d, J = 8.32 Hz, 1H), 7.70 (d, J = 15.92 Hz, 1H), 8.06-8.09 (m, 1H), 8.10 - 8.14 (m, 2H), 8.29-8.33 (m, 2H), 8.60 (dd, J = 4.60, 1.39 Hz, 1H), 8.85 (d, J = 1.80 Hz, 1H), 8.94 (d, J = 2.38 Hz, 1H), 10.68 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyl)acetamide (I-T2267) [0713] Purification by fcc (silica gel, 10% MeOH in DCM) afforded N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -2-(3-pyridyl)acetamide as a light- yellow solid (13.7 mg, 8%). LC-MS (Method 4): Rt = 3.87 min; m/z = 435.17 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.01-0.07 (m, 2H), 0.33-0.41 (m, 2H), 0.83-0.92 (m, 1H), 1.73 (s, 3H), 3.18-3.23 (m, 1H), 3.70-3.75 (m, 1H), 3.78 (s, 2H), 7.37 (dd, J = 7.67, 4.72 Hz, 1H), 7.60 (d, J = 8.32 Hz, 1H), 7.74-7.77 (m, 1H), 8.07 (d, J = 8.80 Hz, 1H), 8.10 (dd, J = 8.25, 2.02 Hz, 1H), 8.18 (dd, J = 8.70, 2.50 Hz, 1H), 8.28 (d, J = 1.95 Hz, 1H), 8.47 (dd, J = 4.78, 1.34 Hz, 1H), 8.54 (d, J =1.73 Hz, 1H), 8.84 (d, J = 2.47 Hz, 1H), 10.62 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyl)cyclo propanecarboxamide (I-T2289) [0714] Purification by fcc (silica gel, 5% MeOH in DCM) afforded N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -2-(3-pyridyl)cyclopropane carboxamide as a white solid (47.64 mg, 64.7%) LC-MS (Method 4): Rt = 4.28, m/z = 461.33 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 0.00 – 0.09 (m, 2H), 0.33 – 0.41 (m, 2H), 0.83 – 0.94 (m, 1H), 1.49 – 1.55 (m, 1H), 1.56 – 1.61 (m, 1H), 1.73 (s, 3H), 2.15 – 2.19 (m, 1H), 2.45-2.53 (m, 1H), 3.21 (dd, J = 13.9, 7.3 Hz, 1H), 3.73 (dd, J = 14.0, 7.0 Hz, 1H), 7.33 (dd, J = 7.9, 4.7 Hz, 1H), 7.57 – 7.62 (m, 2H), 8.07 (d, J = 8.7 Hz, 1H), 8.11 (dd, J = 8.3, 2.0 Hz, 1H), 8.20 (dd, J = 8.7, 2.6 Hz, 1H), 8.28 (d, J = 2.0 Hz, 1H), 8.43 (dd, J = 4.8, 1.6 Hz, 1H), 8.53 (d, J = 2.3 Hz, 1H), 8.85 (d, J = 2.5 Hz, 1H), 10.66 (s, 1H). Scheme 3 Step 1. N-[2-chloro-4-(5-nitro-2-pyridyl)phenyl]-N-(2,2-difluoroethy l)acetamide [0715] Following Scheme 1 – Step 1, starting from 2-bromo-5-nitro-pyridine (500 mg,2.46 mmol) and N-[2-chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)p henyl]-N-(2,2-difluoroethyl) acetamide (974 mg, 2.71 mmol), purification by fcc (silica gel, 30-50% EtOAc in cyclohexane) afforded N-[2-chloro-4-(5-nitro-2-pyridyl)phenyl]-N-(2,2-difluoroethy l)acetamide as a yellow solid (640 mg, 68%). LC-MS (Method 1): Rt = 2.04 min; m/z = 356.03 [M+H] + . Step 2. N-[4-(5-amino-2-pyridyl)-2-chloro-phenyl]-N-(2,2-difluoroeth yl)acetamide [0716] Following Scheme 2 - Step 4A, N-[4-(5-amino-2-pyridyl)-2-chloro-phenyl]-N-(2,2- difluoroethyl)acetamide was obtained as a brown solid (480 mg, 82%). LC-MS (Method 1): Rt = 1.09 min; m/z = 326.06 [M+H] + . 1 H NMR (500 MHz, DMSO-d6) δ 1.78 (s, 3H), 3.59 – 3.70 (m, 1H), 4.21 – 4.32 (m, 1H), 5.66 (s, 2H), 6.19 (tt, J = 55.7, 4.0 Hz, 1H), 7.00 (dd, J = 8.3, 2.8 Hz, 1H), 7.52 (d, J = 8.3 Hz, 1H), 7.75 (d, J = 8.5Hz, 1H), 7.97 (dd, J = 8.3, 1.95 Hz, 1H), 8.04 (d, J = 2.6 Hz, 1H), 8.15 (d, J = 2.0 Hz, 1H). Step 3. N-[6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyl) acetamide (I-T2290) [0717] Following Scheme 2 – Step 5: starting from 2-(3-pyridyl)acetic acid and N-[4-(5-amino-2- pyridyl)-2-chloro-phenyl]-N-(2,2-difluoroethyl)acetamide (62.7 mg, 0.193 mmol), purification by prep HPLC (Method 6) afforded N-[6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyl)acetamide as a white solid (42.9 mg, 60%). LC-MS (Method 5): Rt = 4.42 min, m/z = 445.12 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 1.79 (s, 3H), 3.69 (qd, J = 14.3, 4.5 Hz, 1H), 3.79 (s, 2H), 4.21 – 4.33 (m, 1H), 6.20 (tt, J = 55.7, 4.0 Hz, 1H), 7.37 (dd, J = 7.8, 4.8 Hz, 1H), 7.62 (d, J = 8.3 Hz, 1H), 7.74 – 7.78 (m, 1H), 8.07 (d, J = 8.7 Hz, 1H), 8.12 (dd, J = 8.3, 1.9 Hz, 1H), 8.19 (dd, J = 8.7, 2.4 Hz, 1H), 8.29 (d, J = 1.9 Hz, 1H), 8.45 – 8.50 (m, 1H), 8.54 (d, J = 1.6 Hz, 1H), 8.85 (d, J = 2.4 Hz, 1H), 10.63 (s, 1H). Following Scheme 3 N-[6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyloxy)acetamide (I-T2291) [0718] Purification by prep HPLC (Method 6) afforded N-[6-[4-[acetyl(2,2-difluoroethyl)amino]-3- chloro-phenyl]-3-pyridyl]-2-(3-pyridyloxy)acetamide as a white solid (38.5 mg, 52.7%). LC-MS (Method 5): Rt = 4.79 min, m/z = 461.12 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 1.79 (s, 3H), 3.69 (qd, J = 14.3, 4.5 Hz, 1H), 4.21 – 4.34 (m, 1H), 4.88 (s, 2H), 6.20 (tt, J = 55.7, 4.0 Hz, 1H), 7.37 (dd, J = 8.4, 4.6 Hz, 1H), 7.44 – 7.48 (m, 1H), 7.63 (d, J = 8.3 Hz, 1H), 8.10 (d, J = 8.7 Hz, 1H), 8.13 (dd, J = 8.3, 2.0 Hz, 1H), 8.20 – 8.25 (m, 2H), 8.30 (d, J = 2.0 Hz, 1H), 8.40 (d, J = 2.9 Hz, 1H), 8.91 (d, J = 2.4 Hz, 1H), 10.53 (s, 1H). (E)-N-[6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl ]-3-pyridyl]-3-(3-pyridyl)prop-2- enamide (I-T2293) [0719] Purification by fcc (silica gel, 5% MeOH in DCM) afforded (E)-N-[6-[4-[acetyl(2,2- difluoroethyl)amino]-3-chloro-phenyl]-3-pyridyl]-3-(3-pyridy l)prop-2-enamide as a white solid (20.6 mg, 28.3%). LC-MS (Method 4): Rt = 4.03, m/z = 456.80 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 1.80 (s, 3H), 3.70 (qd, J = 14.3, 4.5 Hz, 1H), 4.22-4.34 (m, 1H), 6.21 (tt, J = 55.7, 4.0 Hz, 1H), 6.96 (d, J = 15.8 Hz, 1H), 7.50 (dd, J = 7.9, 4.8 Hz, 1H), 7.64 (d, J = 8.3 Hz, 1H), 7.70 (d, J = 15.8 Hz, 1H), 8.06 – 8.10 (m, 1H), 8.12 (d, J = 8.7 Hz, 1H), 8.14 (dd, J = 8.3, 2.0 Hz, 1H), 8.30 – 8.34 (m, 2H), 8.61 (dd, J = 4.7, 1.4 Hz, 1H), 8.86 (d, J = 2.0 Hz, 1H), 8.95 (d, J = 2.5 Hz, 1H), 10.69 (s, 1H). N-[6-[4-[acetyl(2,2-difluoroethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(3-pyridyl)cyclopropane carboxamide (I-T2294) [0720] Purification by fcc (silica gel, 5% MeOH in DCM) afforded (N-[6-[4-[acetyl(2,2- difluoroethyl)amino]-3-chloro-phenyl]-3-pyridyl]-2-(3-pyridy l)cyclopropanecarboxamide as a white solid (49.8 mg, 68.6%). LC-MS (Method 4): Rt = 4.12, m/z = 471.27 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ 1.49 – 1.55 (m, 1H), 1.56 – 1.61 (m, 1H), 1.79 (s, 3H), 2.14 – 2.19 (m, 1H), 2.5 (m, 1H), 3.69 (qd, J = 14.2, 4.5 Hz, 1H), 4.22 – 4.33 (m, 1H), 6.20 (tt, J = 55.7, 3.9 Hz, 1H), 7.33 (dd, J = 7.8, 4.8 Hz, 1H), 7.57 – 7.64 (m, 2H), 8.07 (d, J = 8.7 Hz, 1H), 8.12 (dd, J = 8.3, 1.8 Hz, 1H), 8.20 (dd, J = 8.7, 2.4 Hz, 1H), 8.29 (d, J = 1.8 Hz, 1H), 8.41 – 8.44 (m, 1H), 8.53 (d, J = 1.9 Hz, 1H), 8.85 (d, J = 2.3 Hz, 1H), 10.67 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-phenoxy-acetamide (I- T2328) [0721] Purification by fcc (silica gel, 0-50 % EtOAc in cyclohexane) afforded N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -2-phenoxy-acetamide as a white solid (47 mg, 63%). LC-MS (Method 4): Rt = 4.03 min, m/z = 450.04 [M+H] + . δ 0.001-0.08 (m, 2H), 0.33- 0.42 (m, 2H), 0.85-0.94 (m, 1H), 1.73 (s, 3H), 3.18-3.25 (m, 1H), 3.70-3.77 (m, 1H).4.77 (s, 2H), 6.99 (t, J = 7.3Hz, 1H), 7.03 (d, J = 8.0 Hz, 2H), 7.31-7.36 (m, 2H), 7.61 (d, J = 8.3Hz, 1H), 8.10 (d, J = 8.7 Hz, 1H), 8.12 (dd, J = 8.3, 1.9 Hz, 1H), 8.24 (dd, J = 8.7; 2.5 Hz, 1H), 8.30 (d, J = 1.9 Hz, 1H), 8.92 (d, J = 2.3 Hz, 1H), 10.48 (s, 1H). (E)-N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl ]-3-pyridyl]-3-phenyl-prop-2- enamide (I-T2329) [0722] Purification by fcc (silica gel, 0-2.5 % of MeOH in DCM) afforded (E)-N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -3-phenyl-prop-2-enamide as a white solid (29.5 mg, 51%). LC-MS (Method 5b): Rt = 7.05 min, m/z = 446.13 [M+H]+.1H NMR (600 MHz, DMSO-d6): δ -0.02-0.09 (m, 2H), 0.34-0.42 (m, 2H), 0.86-0.93 (m, 1H), 1.74 (s, 3H), 3.22 (dd, J = 14.0, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.0 Hz, 1H), 6.86 (d, J = 15.8 Hz, 1H), 7.42-7.49 (m, 3H), 7.61 (d, J = 8.2 Hz, 1H), 7.64-7.68 (m, 3H), 8.11 (d, J = 8.6 Hz, 1H), 8.13 (dd, J = 8.2, 2.1 Hz, 1H), 8.30 (d, J = 2.1 Hz, 1H), 8.31 (dd, J = 8.5, 2.4 Hz, 1H), 8.94 (d, J = 2.5 Hz, 1H), 10.63 (bs, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-fluoro-2-phenyl- acetamide (I-T2331) [0723] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -2-fluoro-2-phenyl-acetamide as a white solid (29.5 mg, 51%). LC-MS (Method 3): Rt = 4.86 min, m/z = 452.09 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 0.00 - 0.07 (m, 2H), 0.33 - 0.41 (m, 2H), 0.80 - 0.97 (m, 1H), 1.73 (s, 3H), 3.21 (dd, J = 13.7, 7.9 Hz, 1H), 3.73 (dd, J = 14.0, 7.0 Hz, 1H), 6.15 (d, J = 47.0 Hz, 1H), 7.43 - 7.50 (m, 3H), 7.56 - 7.59 (m, 2H), 7.61 (d, J = 8.2Hz, 1H), 8.05 - 8.16 (m, 2H), 8.24 - 8.30 (m, 2H), 8.96 - 8.99 (m, 1H), 10.79 (br s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-3-(2-fluoro-3- pyridyl)propenamide (I-T2369) [0724] Purification by fcc (silica gel, 0-3% MeOH in DCM) afforded N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -3-(2-fluoro-3-pyridyl)propanamide as a white solid (57 mg, 66%). LC-MS (Method 4): Rt = 4.40 min, m/z = 467.17 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.01-0.06 (m, 2H), 0.34-0.41 (m, 2H), 0.85-0.93 (m, 1H), 1.73 (s, 3H), 2.74 (t, J = 7.6 Hz, 2H), 2.92 (t, J = 7.5 Hz, 2H), 3.21 (dd, J = 14.0, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 7.28-7.32 (m, 1H), 7.60 (d, J = 8.3 Hz, 1H), 7.86-7.91 (m, 1H), 8.06 (d, J = 8.7 Hz, 1H), 8.08- 8.11 (m, 2H), 8.15-8.18 (m, 1H), 8.27 (d, J = 2.0 Hz, 1H), 8.80 (d, J = 2.5 Hz, 1H), 10.35 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(4- fluorophenyl)acetamide (I-T2390) [0725] Purification by prep HPLC (Method 6) afforded N-[6-[4-[acetyl(cyclopropylmethyl)amino]- 3-chloro-phenyl]-3-pyridyl]-2-(4-fluorophenyl)acetamide as a white solid (59.7 mg, 73%). LC-MS (Method 5b): Rt = 6.59 min, m/z = 452.16 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.00 - 0.07 (m, 2H), 0.34 - 0.40 (m, 2H), 0.85 - 0.92 (m, 1H), 1.73 (s, 3H), 3.21 (dd, J = 13.9, 7.3 Hz, 1H), 3.72 (s, 2H), 3.73 (dd, J = 13.9, 6.8 Hz, 1H), 7.15 - 7.19 (m, 2H), 7.36 - 7.40 (m, 2H), 7.60 (d, J = 8.3 Hz, 1H), 8.07 (d, J = 8.8 Hz, 1H), 8.10 (dd, J = 8.3, 2.0 Hz, 1H), 8.19 (dd, J = 8.6, 2.3 Hz, 1H), 8.28 (d, J = 2.0 Hz, 1H), 8.84 (d, J = 2.4 Hz, 1H), 10.56 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(6-chloro-3- pyridyl)acetamide (I-T2391) [0726] Purification by fcc (silica gel, 0-3% MeOH in DCM) afforded N-[6-[4- [acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3-pyridyl] -2-(6-chloro-3-pyridyl)acetamide as a white solid (62 mg, 71%). LC-MS (Method 4): Rt = 4.46 min, m/z = 469.04 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.01-0.06 (m, 2H), 0.34-0.41 (m, 2H), 0.85-0.93 (m, 1H), 1.73 (s, 3H), 3.21 (dd, J = 14.0, 7.3 Hz, 1H), 3.73 (dd, J = 13.9, 7.0 Hz, 1H), 3.82 (s, 2H), 7.51 (dd, J = 8.2, 0.4 Hz, 1H), 7.60 (d, J = 8.3 Hz, 1H), 7.84 (dd, J = 8.2, 2.5 Hz, 1H), 8.08 (d, J = 8.6 Hz, 1H), 8.11 (dd, J = 8.3, 2.1 Hz, 1H), 8.18 (dd, J = 8.7, 2.5 Hz, 1H), 8.28 (d, J = 2.0 Hz, 1H), 8.37 (dd, J = 2.5, 0.3 Hz, 1H), 8.84 (dd, J = 2.6, 0.5 Hz, 1H), 10.62 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(2-chloro-3- pyridyl)acetamide (I-T2392) [0727] Purification by prep HPLC (Method 6) afforded N-[6-[4-[acetyl(cyclopropylmethyl)amino]- 3-chloro-phenyl]-3-pyridyl]-2-(2-chloro-3-pyridyl)acetamide as a white solid (54.7 mg, 64%). LC-MS (Method 3): Rt = 4.20 min, m/z = 469.04 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.01-0.07 (m, 2H), 0.34-0.41 (m, 2H), 0.86-0.93 (m, 1H), 1.73 (s, 3H), 3.21 (dd, J = 14.1, 7.3 Hz, 1H), 3.74 (dd, J = 14.0, 7.0 Hz, 1H), 3.95 (s, 2H), 7.45 (dd, J = 7.5, 4.8 Hz, 1H), 7.61 (d, J = 8.3 Hz, 1H), 7.91 (dd, J = 7.5, 1.8 Hz, 1H), 8.08 (d, J = 8.7 Hz, 1H), 8.11 (dd, J = 8.3, 2.0 Hz, 1H), 8.18 (dd, J = 8.7, 2.5 Hz, 1H), 8.29 (d, J = 2.0 Hz, 1H), 8.35 (dd, J = 4.8, 1.9 Hz, 1H), 8.86 (d, J = 2.5 Hz, 1H), 10.68 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-(4- chlorophenyl)acetamide (I-T2393) [0728] Purification by prep HPLC (Method 7) afforded N-[6-[4-[acetyl(cyclopropylmethyl)amino]- 3-chloro-phenyl]-3-pyridyl]-2-(4-chlorophenyl)acetamide as a white solid (40 mg, 47%). LC-MS (Method 3): Rt = 5.07 min, m/z = 468.1 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.02 - 0.06 (m, 2H), 0.34 - 0.42 (m, 2H), 0.85 - 0.93 (m, 1H), 1.73 (s, 3H), 3.21 (dd, J = 14.0, 7.2 Hz, 1H), 3.70 - 3.78 (m, 3H), 7.36 - 7.38 (m, 2H), 7.39 - 7.42 (m, 2H), 7.60 (d, J = 8.3 Hz, 1H), 8.07 (d, J = 8.8 Hz, 1H), 8.10 (dd, J = 8.3, 2.0 Hz, 1H), 8.18 (dd, J = 8.8, 2.6 Hz, 1H), 8.28 (d, J = 2.0 Hz, 1H), 8.84 (d, J = 2.4 Hz, 1H), 10.57 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-2-cyclohexyl-acetamide (I-T2394) [0729] Purification by prep HPLC (Method 6) afforded N-[6-[4-[acetyl(cyclopropylmethyl)amino]- 3-chloro-phenyl]-3-pyridyl]-2-cyclohexyl-acetamide as a white solid (27.7 mg, 35%). LC-MS (Method 5b): Rt = 7.29 min, m/z = 440.2 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): 0.00 - 0.07 (m, 2H), 0.34 - 0.40 (m, 2H), 0.85 - 0.93 (m, 1H), 0.95 - 1.03 (m, 2H), 1.10 - 1.18 (m, 1H), 1.19 - 1.28 (m, 2H), 1.59 - 1.64 (m, 1H), 1.64 - 1.74 (m, 7H), 1.75 - 1.84 (m, 1H), 2.25 (d, J = 7.2 Hz, 2H), 3.21 (dd, J = 14.0, 7.2 Hz, 1H), 3.73 (dd, J = 14.0, 7.1 Hz, 1H), 7.59 (d, J = 8.3 Hz, 1H), 8.05 (d, J = 8.6 Hz, 1H), 8.10 (dd, J = 8.3, 2.0 Hz, 1H), 8.19 (dd, J = 8.8, 2.6 Hz, 1H), 8.27 (d, J = 2.0 Hz, 1H), 8.83 (d, J = 2.4 Hz, 1H), 10.24 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-chloro-phenyl]-3- pyridyl]-3-(6-fluoro-3- pyridyl)propanamide (I-2384) [0730] Purification by prep HPLC (Method 6) afforded N-[6-[4-[acetyl(cyclopropylmethyl)amino]- 3-chloro-phenyl]-3-pyridyl]-3-(6-fluoro-3-pyridyl)propanamid e as a white solid (22.7 mg, 27%). LC- MS (Method 5b): Rt = 6.04 min, m/z = 467.18 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ - 0.00 - 0.07 (m, 2H), 0.34 - 0.42 (m, 2H), 0.86 - 0.93 (m, 1H), 1.73 (s, 3H), 2.78 (t, J = 7.6 Hz, 2H), 3.02 (t, J = 7.5 Hz, 2H), 3.21 (dd, J = 13.9, 7.2 Hz, 1H), 3.74 (dd, J = 14.0, 7.1 Hz, 1H), 7.10 (s, 1H), 7.27 - 7.30 (m, 1H), 7.60 (d, J = 8.3 Hz, 1H), 8.06 (d, J = 8.6 Hz, 1H), 8.10 (dd, J = 8.3, 2.0 Hz, 1H), 8.14 - 8.19 (m, 2H), 8.28 (d, J = 2.0 Hz, 1H), 8.81 (d, J = 2.6 Hz, 1H), 10.36 (s, 1H). N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-3- pyridyl]-3-(2-fluoro-3- pyridyl)propenamide (I-T2416) [0731] Step 1. N-(cyclopropylmethyl)-N-[2-fluoro-4-(5-nitro-2-pyridyl)pheny l]acetamide [0732] Following Scheme 1 – Step 1, starting from 2-bromo-5-nitro-pyridine (200 mg, 0.98 mmol) and N-(cyclopropylmethyl)-N-[2-fluoro-4-(4,4,5,5-tetramethyl-1,3 ,2-dioxaborolan-2- yl)phenyl]acetamide (401 mg, 1.08 mmol), purification by fcc (silica gel, 30-50% EtOAc in cyclohexane) afforded N-(cyclopropylmethyl)-N-[2-fluoro-4-(5-nitro-2-pyridyl)pheny l]acetamide as a yellow oil that solidifies upon standing (299 mg, 90%). LC-MS (Method 9): Rt = 1.08 min; m/z = 330.05 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ 0.01 – 0.08 (m, 2H), 0.31 – 0.41 (m, 2H), 0.84 – 0.91 (m, 1H), 1.80 (s, 3H), 3.46 – 3.62 (m, 2H), 7.69 (t, J = 8.1 Hz, 1H), 8.17 (d, J = 8.4 Hz, 1H), 8.22 (d, J = 11.46 Hz, 1H), 8.39 (d, J = 8.7 Hz, 1H), 8.71 (dd, J = 8.8, 2.5 Hz, 1H), 9.47 (d, J = 2.5 Hz, 1H). [0733] Step 2. N-[4-(5-amino-2-pyridyl)-2-fluoro-phenyl]-N-(cyclopropylmeth yl)acetamide [0734] Following Scheme 2- Step 4, N-[4-(5-amino-2-pyridyl)-2-fluoro-phenyl]-N- (cyclopropylmethyl)acetamide was obtained as a yellow solid (246 mg, 88%). LC-MS (Method 9): Rt = 0.85 min; m/z = 300.12 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ – 0.04 – 0.07 (m, 2H), 0.3 – 0.4 (m, 2H), 0.81 – 0.90 (m, 1H), 1.77 (s, 3H), 3.47 (d, J = 7.2 Hz, 2H), 5.6 (s, 2H), 7.00 (dd, J = 8.5, 2.8 Hz, 1H), 7.45 (t, J = 8.2 Hz, 1H), 7.73 (d, J = 8.5 Hz, 1H), 7.81 – 7.88 (m, 2H), 8.03 (d, J = 2.7 Hz, 1H). [0735] N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-3- pyridyl]-3-(2-fluoro-3- pyridyl)propenamide (I-T2416) [0736] Following Scheme 1- Step 5, purification by fcc (silica gel, 0-10% MeOH in DCM) afforded N-[6-[4-[acetyl(cyclopropylmethyl)amino]-3-fluoro-phenyl]-3- pyridyl]-3-(2-fluoro-3- pyridyl)propenamide as a white solid (40.6 mg, 50%). LC-MS (Method 1): Rt = 1.77 min, m/z = 451.22 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): -0.03 - 0.07 (m, 2H), 0.32 - 0.40 (m, 2H), 0.83 - 0.90 (m, 1H), 1.78 (s, 3H), 2.73 (t, J = 7.5 Hz, 2H), 2.96 (t, J = 7.4 Hz, 2H), 3.49 (dd, J = 7.2, 2.6 Hz, 2H), 7.28 - 7.31 (m, 1H), 7.55 (t, J = 8.3 Hz, 1H), 7.85 - 7.91 (m, 1H), 7.97 (dd, J = 8.3, 1.8 Hz, 1H), 8.01 (dd, J = 11.6, 1.8 Hz, 1H), 8.03 (d, J = 8.7 Hz, 1H), 8.08 – 8.1 (m, 1H), 8.15 (dd, J = 8.7, 2.5 Hz, 1H), 8.79 (d, J = 2.4 Hz, 1H), 10.34 (s, 1H). Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[3-(2-fluoro-3-pyrid yl)propanoylamino]-2- pyridyl]benzoate (I-T2415) [0737] Step 1. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(5-nitro-2-pyridyl)benz oate [0738] Following Scheme 1 – Step 1, starting from 2-bromo-5-nitro-pyridine (335 mg, 1.65 mmol) and methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(4,4,5,5-tetramethyl-1, 3,2-dioxaborolan-2- yl)benzoate (750 mg, 1.65 mmol) using Pd(dppf)Cl 2 ×DCM (68 mg, 0.08 mmol) as a catalyst, purification by fcc (silica gel, 0-100% EtOAc in cyclohexane) afforded methyl 2-[acetyl(cyclopropyl methyl)amino]-5-(5-nitro-2-pyridyl)benzoate as a yellow oil (535 mg, 87%). LC-MS (Method 9): Rt = 1.09 min; m/z = 370.16 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ -0.09 - 0.02 (m, 2H), 0.29 - 0.37 (m, 2H), 0.76 - 0.87 (m, 1H), 1.72 (s, 3H), 3.25 (dd, J = 14.1, 7.2 Hz, 1H), 3.62 (dd, J = 14.1, 7.2 Hz, 1H), 3.86 (s, 3H), 7.67 (d, J = 8.3 Hz, 1H), 8.41 (dd, J = 8.8, 0.7 Hz, 1H), 8.51 (dd, J = 8.3, 2.3 Hz, 1H), 8.71 (dd, J = 8.8, 2.7 Hz, 1H), 8.77 (d, J = 2.1 Hz, 1H), 9.50 (dd, J = 2.7, 0.6 Hz, 1H). [0739] Step 2. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(5-amino-2-pyridyl)benz oate [0740] Following Scheme 2 - Step 4, methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(5-amino-2- pyridyl)benzoate was obtained as a yellow solid (484 mg, 98%). LC-MS (Method 9): Rt = 0.81 min; m/z = 340.26 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ -0.09 - 0.03 (m, 2H), 0.28 - 0.37 (m, 2H), 0.74 - 0.86 (m, 1H), 1.69 (s, 3H), 3.18 (dd, J = 13.9, 7.1 Hz, 1H), 3.60 (dd, J = 14.0, 7.2 Hz, 1H), 3.82 (s, 3H), 5.63 (s, 2H), 7.02 (dd, J = 8.6, 2.8 Hz, 1H), 7.44 (d, J = 8.3 Hz, 1H), 7.74 (d, J = 8.6 Hz, 1H), 8.04 - 8.07 (m, 1H), 8.17 (dd, J = 8.3, 2.2 Hz, 1H), 8.49 (d, J = 2.2 Hz, 1H). [0741] Step 3. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[3-(2-fluoro-3-pyrid yl)propanoyl amino]-2-pyridyl]benzoate (I-T2415) [0742] Following Scheme 1 – Step 5: starting from 3-(2-fluoro-3-pyridyl)propanoic acid (31 mg, 0.18 mmol) and methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(5-amino-2-pyridyl)benz oate (50 mg, 0.14 mmol), purification by fcc (silica gel, 0-10% MeOH in DCM) afforded methyl 2- [acetyl(cyclopropylmethyl)amino]-5-[5-[3-(2-fluoro-3-pyridyl )propanoylamino]-2-pyridyl]benzoate as a white solid (38 mg, 53%). LC-MS (Method 1): Rt = 1.69 min, m/z = 491.11 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.08 - 0.00 (m, 2H), 0.29 - 0.36 (m, 2H), 0.77 - 0.85 (m, 1H), 1.70 (s, 3H), 2.72 - 2.76 (m, 2H), 2.97 (t, J = 7.5 Hz, 2H), 3.21 (dd, J = 14.1, 7.2 Hz, 1H), 3.61 (dd, J = 14.1, 7.2 Hz, 1H), 3.84 (s, 3H), 7.29 - 7.32 (m, 1H), 7.54 (d, J = 8.3 Hz, 1H), 7.86 - 7.90 (m, 1H), 8.05 (d, J = 8.6 Hz, 1H), 8.08 - 8.10 (m, 1H), 8.18 (dd, J = 8.7, 2.5 Hz, 1H), 8.31 (dd, J = 8.3, 2.3 Hz, 1H), 8.60 (d, J = 2.2 Hz, 1H), 8.81 (d, J = 2.2 Hz, 1H), 10.35 (s, 1H). Scheme 4 Intermediate A was first prepared in 2 steps as follows: [0743] Following Scheme 1 - Step 2a: Methyl 2-(cyclopropylmethylamino)-5-(4,4,5,5-tetramethyl- 1,3,2-dioxaborolan-2-yl)benzoate was obtained as a white solid (1.02 g, 94%). LC-MS (Method 9): Rt = 1.53 min; m/z = 332.05 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 0.22-0.28 (m, 2H), 0.47-0.54 (m, 2H), 1.02-1.15 (m, 1H), 1.25 (s, 12H), 3.07 (dd, J = 6.66 Hz, J = 5.20 Hz, 2H), 3.80 (s, 3H), 6.73 (d, J = 8.50 Hz, 1H), 7.59 (dd, J = 8.50 Hz, J = 1.29 Hz, 1H), 8.01 (t, J = 4.87 Hz, 1H), 8.15 (d, J = 1.48 Hz, 1H). b. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(4,4,5,5-tetramethyl-1, 3,2-dioxaborolan-2-yl) benzoate [0744] Following Scheme 1 - Step 3a: methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(4,4,5,5- tetramethyl-1,3,2-dioxaborolan-2-yl)benzoate was obtained as a white solid (1.0 g, 91%). LC-MS (Method 9): Rt = 0.63 min; m/z = 374.2 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ -0.12 - -0.03 (m, 2H), 0.27-0.33 (m, 2H), 0.72-0.80 (m, 1H), 1.31 (s, 12H), 1.63 (s, 3H), 3.13-3.19 (m, 1H), 3.55-3.61 (m, 1H), 3.80 (s, 3H), 7.46 (d, J = 7.70 Hz, 1H), 7.93 (dd, J = 7.70 Hz, J = 1.37 Hz, 1H), 8.20 (d, J = 1.30 Hz, 1H). Step 1.6-Bromopyridine-3-carboxylic acid [0745] Methyl 6-bromopyridine-3-carboxylate (2.0 g, 9.3 mmol) was dissolved in THF (50 mL). LiOH (1.46 g, 56 mmol) was dissolved in water (10 mL) and added to the RM which was stirred at RT overnight. THF was removed in vacuo. The aqueous layer was diluted with water (15 mL) and acidified with 2M HCl to pH = 2. The resulting precipitate was collected by filtration and dried in a vacuum oven overnight to afford 6-bromopyridine-3-carboxylic acid as a white solid (1.85 g, 99%). LC-MS (Method 8): Rt = 0.54 min, m/z = 203.91 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 7.79 (d, J = 8.44 Hz, 1H), 8.15 (dd, J = 8.15 Hz, J = 2.45 Hz, 1H), 8.83 (d, J = 2.28 Hz, 1H), 13.63 (bs, 1H). Step 2.6-Bromo-N-(3-pyridylmethyl)pyridine-3-carboxamide [0746] Following Scheme 1 - Step 5: 6-bromo-N-(3-pyridylmethyl)pyridine-3-carboxamide was obtained as a white solid (10.3 g, 81%). LC-MS (Method 2): Rt = 0.99 min, m/z = 293.95 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 4.5 (d, J = 5.72 Hz, 2H), 7.32-7.39 (m, 1H), 7.73 (dt, J = 7.83, 1.8 Hz, 1H), 7.78 (dd, J = 8.35, 0.54 Hz, 1H), 8.14 (dd, J = 8.30, 2.57 Hz, 1H), 8.46 (dd, J = 4.73, 1.45 Hz, 1H), 8.55 (d, J = 1.75 Hz, 1H), 8.80 – 8.84 (m, 1H), 9.32 (t, J = 5.71 Hz, 1H). Step 3a. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[2-(3-pyridyl)ethylc arbamoyl]-2- pyridyl]benzoate (I-T2296) [0747] To a solution of 6-bromo-N-(2-pyridylmethyl)pyridine-3-carboxamide (70.4 mg, 0.241 mmol) and methyl 2-[acetyl(cyclopropylmethyl)amino]-5-(4,4,5,5-tetramethyl-1, 3,2-dioxaborolan-2- yl)benzoate (90 mg, 0.241 mmol) in a mixture of 1,4-dioxane (4.5 mL) and water (0.45 mL), was added K 3 PO 4 (154 mg, 0.723 mmol) was added. The RM was degassed with argon for 5 min, then Pd(dppf)Cl 2 .DCM (10 mg, 0.012 mmol) was added. The RM was stirred at 70 °C overnight. It was then cooled to RT and the organic layer was concentrated in vacuo. The resulting residue was dissolved in EtOAc and washed with NaHCO 3 and brine. The organic layer was evaporated to give the crude product which was purified by fcc (silica gel, 0-10% MeOH in DCM) to afford methyl 2- [acetyl(cyclopropylmethyl)amino]-5-[5-[2-(3-pyridyl)ethylcar bamoyl]-2-pyridyl]benzoate as a white solid (33 mg, 29%). LC-MS (Method 4): Rt = 3.54 min, m/z = 473.27 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ -0.07-0.02 (m, 2H), 0.26-0.37 (m, 2H), 0.72-0.86 (m, 1H), 1.70 (s, 3H), 2.89 (t, J = 6.9 Hz, 2H), 3.17-3.26 (m, 1H), 3.50-3.65 (m, 3H), 3.84 (s, 3H), 7.31 (dd, J = 7.6 Hz, J = 4.7 Hz, 1H), 7.60 (d, J = 8.3 Hz, 1H), 7.68 (dt, J =7.8 Hz, J = 1.8 Hz, 1H), 8.19 (d, J = 8.3 Hz, 1H), 8.27 (dd, J = 8.3 Hz, J = 2.2 Hz, 1H), 8.39-8.45 (m, 2H), 8.46 (d, J = 1.8 Hz, 1H), 8.68 (d, J = 2.2 Hz, 1H), 8.84 (t, J = 5.6 Hz, 1H), 9.06 (d, J = 1.7 Hz, 1H). Step 3b. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-(3-imidazol-1-ylprop ylcarbamoyl)-2- pyridyl]benzoate (I-T2300) [0748] The reaction conditions are performed similarly to Step 3a. In this case, the RM was heated in a microwave reactor at 80 °C for 30 minutes. Purification by fcc (silica gel, 0-10% MeOH in DCM) afforded methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-(3-imidazol-1-ylprop ylcarbamoyl)-2- pyridyl]benzoate as a white solid (25.1 mg, 25%). LC-MS (Method 4): Rt = 3.32 min, m/z = 476.4 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 0.00 – 0.02 (m, 2H), 0.30 – 0.36 (m, 2H), 0.77 – 0.87 (m, 1H), 1.72 (s, 3H), 1.95 – 2.04 (m, 2H), 3.23 (dd, J = 13.8, 7.4 Hz, 1H), 3.28 (m, 2H), 3.63 (dd, J = 14.0, 7.0 Hz, 1H), 3.85 (s, 3H), 4.06 (t, J = 6.5 Hz, 2H), 6.90 (s, 1H), 7.22 (s, 1H), 7.62 (d, J = 8.2 Hz, 1H), 7.68 (s, 1H), 8.23 (d, J = 8.2 Hz, 1H), 8.30 – 8.35 (m, 1H), 8.43 – 8.47 (m, 1H), 8.71 (s, 1H), 8.75 – 8.80 (m, 1H), 9.12 (s, 1H). Following Scheme 4 Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[2-(1H-pyrazol-4-yl) ethylcarbamoyl]-2- pyridyl]benzoate (I-T2297) [0749] Following Step 3b: purification by column chromatography over silica gel (0-10% MeOH in DCM) afforded methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[2-(1H-pyrazol-4- yl)ethylcarbamoyl]-2-pyridyl]benzoate as a white solid (9.87 mg, 9.9%). LC-MS (Method 4): Rt = 3.30 min, m/z = 462.03 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ -0.08-0.01 (m, 2H), 0.27-0.37 (m, 2H), 0.76-0.86 (m, 1H), 1.71 (s, 3H), 2.73 (t, J = 7.5 Hz, 2H), 3.19-3.26 (m, 1H), 3.44-3.49 (m, 2H), 3.59-3.65 (m, 1H), 3.84 (s, 3H), 7.31-7.64 (m, 3H), 8.21 (d, J = 8.5 Hz, 1H), 8.31 (dd, J = 8.1 Hz, J = 2.1 Hz, 1H), 8.44 (dd, J = 8.2 Hz, J = 2.0 Hz, 1H), 8.70 (d, J = 2.2 Hz, 1H), 8.82 (t, J = 5.4 Hz, 1H), 9.10 (d, J = 1.8 Hz, 1H), 12.56 (bs, 1H). 6-[4-[Acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]-N-[2-( 3-pyridyl)ethyl]pyridine-3- carboxamide (I-T2305) [0750] Following Scheme 4 – Step 3b, starting from 6-bromo-N-[2-(3-pyridyl)ethyl]pyridine-3- carboxamide (42 mg, 0.137 mmol) and N-[2-cyano-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)phenyl]-N-(cyclopropylmethyl)acetamide (50 mg, 63%, 0.09 mmol), purification by fcc (silica gel, 0-10% MeOH in DCM) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]-N-[2-( 3- pyridyl)ethyl]pyridine-3-carboxamide as a white solid (21.2 mg, 52%). LC-MS (Method 4): Rt = 3.54 min, m/z = 439.95 [M+H]+. 1 H NMR (500 MHz, DMSO-d 6 ): δ -0.03-0.08 (m, 2H), 0.34-0.43 (m, 2H), 0.83-0.94 (m, 1H), 1.81 (s, 3H), 2.90 (t, J = 7.1 Hz, 2H), 3.52-3.59 (m, 3H), 3.62-3.68 (m, 1H), 7.33 (dd, J = 7.7 Hz, J = 4.0 Hz, 1H), 7.68 (d, J = 7.7 Hz, 1H), 7.78 (d, J = 8.2 Hz, 1H), 8.22-8.31 (m, 2H), 8.42 (d, J = 5.02 Hz, 1H), 8.47 (bs, 1H), 8.58 (d, J = 8.0 Hz, 1H), 8.72 (bs, 1H), 8.87 (t, J = 5.5 Hz, 1H), 9.10 (bs, 1H). Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-(5-fluoroisoindoline -2-carbonyl)-2-pyridyl] benzoate (I-T2421) [0751] Step 2. (6-bromo-3-pyridyl)-(5-fluoroisoindolin-2-yl)methanone [0752] Following Scheme 1 - Step 5: purification by fcc (silica gel, 0-5% MeOH in DCM) afforded methyl (6-bromo-3-pyridyl)-(5-fluoroisoindolin-2-yl)methanone as a yellow solid (180 mg, 85%). LC-MS (Method 8): Rt = 0.97 min, m/z = 321.00 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 4.75- 4.89 (m, 4H), 7.09-7.46 (m, 3H), 7.77-7.81 (m, 1H), 8.00 (dd, J = 8.2, 2.5 Hz, 1H), 8.65 (dd, J = 2.4, 0.7 Hz, 1H). [0753] Step 3b. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-(5-fluoroisoindoline -2-carbonyl)-2- pyridyl]benzoate (I-T2421) [0754] Purification by fcc (silica gel, 0-5% MeOH in DCM) afforded methyl 2-[acetyl(cyclopropyl methyl)amino]-5-[5-(5-fluoroisoindoline-2-carbonyl)-2-pyridy l]benzoate as a white solid (57 mg, 53%). LC-MS (Method 2): Rt = 1.99 min, m/z = 488.19 [M+H] + .1H NMR (600 MHz, DMSO-d 6 ): δ - 0.07-0.02 (m, 2H), 0.30-0.37 (m, 2H), 0.79-0.86 (m, 1H), 1.72 (s, 3H), 3.24 (dd, J = 14.1, 7.2 Hz, 1H), 3.63 (dd, J = 14.1, 7.2 Hz, 1H), 3.86 (s, 3H), 4.88 (dd, J = 20.1, 15.3 Hz, 4H), 7.11-7.47 (m, 3H), 7.62 (d, J = 8.2 Hz, 1H), 8.16-8.24 (m, 2H), 8.45 (dd, J = 8.3, 2.3 Hz, 1H), 8.73 (d, J = 2.3 Hz, 1H), 8.96-8.97 (m, 1H). Scheme 4- Side Products [0755] I-T2306 and 2314 were prepared following Scheme 4. The following 2 intermediates were isolated during their synthesis: Methyl 6-[4-[acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]pyridin e-3-carboxylate [0756] Purification by fcc (silica gel, 1% MeOH in DCM) afforded methyl 6-[4- [acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]pyridine-3-c arboxylate as a yellow oil (207 mg, 96%). LC-MS (Method 8): Rt = 1.07 min, m/z = 350.22 [M+H] + . 6-[4-[acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]pyridin e-3-carboxylic acid [0757] 6-[4-[acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]pyridin e-3-carboxylic acid is obtained as a yellow solid (162 mg, 67%). LC-MS (Method 8): Rt = 0.84 min, m/z = 336.15 [M+H] + . Scheme 4a [0758] The reaction procedures from Scheme 4 were followed, although the reaction steps were in a different order, per below: [0759] Intermediate A is prepared in the same way as described for Scheme 4 [0760] Step 1 is the same as Scheme 4 - Step 3a (Suzuki with ester analogue) [0761] Step 2 is the same as Scheme 1 - Step 4 (hydrolysis) [0762] Step 3 is the same as Scheme 1 - Step 5 (amidation) Following Scheme 4a 6-[4-[acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]-N-[3-( 1H-pyrazol-4-yl)propyl]pyridine- 3-carboxamide (I-T2306) [0763] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- cyano-phenyl]-N-[3-(1H-pyrazol-4-yl)propyl]pyridine-3-carbox amide as a white solid (14.5 mg, 7%). LC-MS (Method 5): Rt = 4.94 min, m/z = 443.14 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.02 - 0.09 (m, 2H), 0.35 - 0.44 (m, 2H), 0.86 - 0.93 (m, 1H), 1.77 - 1.81 (m, 2H), 1.81 (s, 3H), 2.49 - 2.50 (m, 2H), 3.32 - 3.33 (m, 2H), 3.56 (dd, J = 14.1, 7.3Hz, 1H), 3.65 (dd, J = 14.1, 7.2 Hz, 1H), 7.36 (s, 1H), 7.54 (br s, 1H), 7.79 (d, J = 8.4 Hz, 1H), 8.28 (d, J = 8.1 Hz, 1H), 8.35 (dd, J = 8.3, 2.2 Hz, 1H), 8.59 (dd, J = 8.3, 2.1 Hz, 1H), 8.73 (d, J = 2.0 Hz, 1H), 8.76 (br t, J = 5.6 Hz, 1H), 9.13 (d, J = 1.8 Hz, 1H), 12.52 (br s, 1H). 6-[4-[acetyl(cyclopropylmethyl)amino]-3-cyano-phenyl]-N-[3-( 1H-pyrazol-5-yl)propyl]pyridine- 3-carboxamide (I-T2313) [0764] Purification by prep HPLC (Method 7) afforded 6-[4-[acetyl(cyclopropylmethyl)amino]-3- cyano-phenyl]-N-[3-(1H-pyrazol-5-yl)propyl]pyridine-3-carbox amide as a white solid (13 mg, 6%). LC-MS (Method 3): Rt = 3.39 min, m/z = 443.3 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.02 - 0.10 (m, 2H), 0.34 - 0.45 (m, 2H), 0.87 - 0.93 (m, 1H), 1.81 (s, 3H), 1.88 (quin, J = 7.4 Hz, 2H), 2.63 - 2.70 (m, 2H), 3.34 - 3.37 (m, 2H), 3.56 (dd, J = 13.8, 7.2 Hz, 1H), 3.65 (dd, J = 14.1 Hz, 7.2Hz, 1H), 6.08 (s, 1H), 7.48 (br s, 1H), 7.79 (d, J = 8.4 Hz, 1H), 8.28 (d, J = 8.3 Hz, 1H), 8.35 (dd, J = 8.4, 2.2 Hz, 1H), 8.59 (dd, J = 8.4, 2.0 Hz, 1H), 8.73 (d, J = 2.0 Hz, 1H), 8.79 (t, J = 5.1 Hz, 1H), 9.13 (d, J = 1.8 Hz, 1H), 12.47 (br s, 1H). Scheme 5 Step 1.6-[4-[Acetyl(cyclopropylmethyl)amino]-3-methoxycarbonyl-ph enyl]pyridine-3- carboxylic acid [0765] Following Scheme 4 – Step 3b: trituration of the crude with ACN afforded 6-[4- [acetyl(cyclopropylmethyl)amino]-3-methoxycarbonyl-phenyl]py ridine-3-carboxylic acid as a white solid (140 mg, 89%). LC-MS (Method 8): Rt = 0.85 min, m/z = 368.87 [M+H] + . Step 2. Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-(imidazo[1,2-a]pyrim idin-6- ylcarbamoyl)-2-pyridyl]benzoate (I-T2295) [0766] Following Scheme 1 – Step 5: Purification by fcc (silica gel, 0-15% MeOH in DCM) afforded methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-(imidazo[1,2-a]pyrim idin-6-ylcarbamoyl)-2- pyridyl]benzoate as a white amorphous solid (16 mg, 30%). LC-MS (Method 4): Rt = 3.34 min, m/z = 484.94 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 0.00 – 0.03 (m, 2H), 0.30 – 0.38 (m, 2H), 0.79 – 0.88 (m, 1H), 1.73 (s, 3H), 3.24 (dd, J = 14.1, 7.2 Hz, 1H), 3.64 (dd, J = 14.1, 7.1 Hz, 1H), 3.87 (s, 3H), 7.65 (d, J = 8.3 Hz, 1H), 7.72 (s, 1H), 8.05 (s, 1H), 8.34 (d, J = 8.3 Hz, 1H), 8.48 – 8.53 (m, 2H), 8.74 (dd, J = 9.9, 2.3 Hz, 2H), 9.29 (d, J = 1.7 Hz, 1H), 9.68 (d, J = 2.6 Hz, 1H), 10.90 (s, 1H). Following Scheme 5: Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[3-(1H-pyrazol-4-yl) propylcarbamoyl]-2- pyridyl]benzoate (I-T2298) [0767] Purification by fcc (silica gel, 0-15% MeOH in DCM) afforded methyl 2- [acetyl(cyclopropylmethyl)amino]-5-[5-[3-(1H-pyrazol-4-yl)pr opylcarbamoyl]-2-pyridyl]benzoate as a white solid (5 mg, 7%). LC-MS (Method 4): Rt = 3.45 min, m/z = 475.98 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ -0.07-0.015 (m, 2H), 0.28-0.36 (m, 2H), 0.77-0.87 (m, 1H), 1.71 (s, 3H), 1.76- 1.84 (m, 2H), 2.5 (m, 2H), 3.20 – 3.26 (m, 1H), 3.33-3.36 (m, 2H), 3.59-3.65 (m, 1H), 3.85 (s, 3H), 7.32-7.56 (bm, 2H), 7.61 (d, J = 8.25 Hz, 1H), 8.21 (d, J = 8.39 Hz, 1H), 8.32 (dd, J = 8.31, 2.24 Hz, 1H), 8.44 (dd, J = 8.43, 2.1 Hz, 1H), 8.7 (d, J = 2.07 Hz, 1H), 8.74 (t, J = 5.59 Hz, 1H), 9.11 (d, J = 1.98 Hz, 1H), 12.5 (br s, 1H). Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[3-(1H-pyrazol-3-yl) propylcarbamoyl]-2- pyridyl]benzoate (I-T2299) [0768] Purification by fcc (silica gel, 0-15% MeOH in DCM) afforded methyl 2- [acetyl(cyclopropylmethyl)amino]-5-[5-[3-(1H-pyrazol-3-yl)pr opylcarbamoyl]-2-pyridyl]benzoate as a white solid (10 mg, 15%). LC-MS (Method 4): Rt = 3.49 min, m/z = 475.98 [M+H] + . 1 H-NMR (500 MHz, DMSO-d 6 ): δ -0.07 to -0.008 (m, 2H), 0.29-0.36 (m, 2H), 0.77-0.86 (m, 1H), 1.7 (s, 3H), 1.83- 1.91 (m, 2H), 2.60-2.69 (m, 2H), 3.19-3.26 (m, 1H), 3.32-3.38 (m, 2H), 3.58 - 3.66 (m, 1H), 3.85 (s, 3H), 6.07 (bs, 1H), 7.27-7.59 (m, 1H), 7.60 (d, J = 7.16 Hz, 1H), 8.21 (d, J = 8.28 Hz, 1H), 8.32 (dd, J = 8.28, 2.16 Hz, 1H), 8.44 (dd, J = 8.32, 2.28 Hz, 1H), 8.70 (d, J = 2.14 Hz, 1H), 8.77 (t, J = 5.56 Hz, 1H), 9.12 (d, J = 2 Hz, 1H), 12.4 (bd, J = 45 Hz, 1H). Methyl 2-[acetyl(cyclopropylmethyl)amino]-5-[5-[2-(2-chloro-4-pyrid yl)ethylcarbamoyl]-2- pyridyl]benzoate (I-T2404) [0769] Purification by fcc (silica gel, 0-10% MeOH in DCM) afforded methyl 2- [acetyl(cyclopropylmethyl)amino]-5-[5-[2-(2-chloro-4-pyridyl )ethylcarbamoyl]-2-pyridyl]benzoate as a white solid (39.2 mg, 58%). LC-MS (Method 1): Rt = 1.70 min, m/z = 507.16 [M+H] + . 1 H NMR (600 MHz, DMSO-d 6 ): δ -0.07-0.01 (m, 2H), 0.29-0.36 (m, 2H), 0.78-0.85 (m, 1H), 1.71 (s, 3H), 2.93 (t, J = 6.9 Hz, 2H), 3.23 (dd, J = 14.1, 7.3 Hz, 1H), 3.57-3.64 (m, 3H), 3.85 (s, 3H), 7.32 (dd, J = 5.07, 1.3 Hz, 1H), 7.44-7.46 (m, 1H), 7.61 (d, J = 8.27 Hz, 1H), 8.21 (dd, J = 8.3, 0.7 Hz, 1H), 8.26 (dd, J = 8.3, 2.2 Hz, 1H), 8.31 (dd, J = 5.0, 0.5 Hz, 1H), 8.44 (dd, J = 8.2, 2.2 Hz, 1H), 8.7 (d, J = 2.2 Hz, 1H), 8.84 (t, J = 5.6 Hz, 1H), 9.06 (dd, J = 2.3, 0.7 Hz, 1H). Following Route E (step e4 with V-o, step e5 with Ac 2 O) 6-[4-[acetyl(oxetan-3-ylmethyl)amino]-3-chloro-phenyl]-N-ben zyl-pyridine-3-carboxamide (I- T2327) [0770] Purification by fcc (silica gel, 10% MeOH in DCM) afforded 6-[4-[acetyl(oxetan-3- ylmethyl)amino]-3-chloro-phenyl]-N-benzyl-pyridine-3-carboxa mide as a white solid (93 mg, 52%). LC-MS (Method 4): Rt = 3.44 min; m/z = 450.41 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.71 (s, 3H), 2.98 -3.15 (m, 1H), 3.77 - 3.90 (m, 1H), 4.0 - 4.11 (m, 1H), 4.12 - 4.26 (m, 2H), 4.45 - 4.61 (m, 4H), 7.19 - 7.29 (m, 1H), 7.3 - 7.37 (m, 4H), 7.60 (d, J = 8.18 Hz, 1H), 8.17 - 8.22 (m, 1H), 8.22 - 8.26 (m, 1H), 8.36 (dd, J = 8.4 Hz, 2.07, 1H), 8.4 (d, J = 2.07 Hz, 1H), 9.15 (d, J = 2Hz, 1H), 9.31 (t, J = 5.83 Hz, 1H). First Exemplary Synthesis of a Compound of Formula V-b3 [0771] 2-Chloro-N-(cyclopropylmethyl)-4-(4,4,5,5-tetramethyl-1,3,2- dioxaborolan-2-yl)aniline (I- T2312) [0772] Following Scheme 1 – Step 2a: 2-chloro-N-(cyclopropylmethyl)-4-(4,4,5,5-tetramethyl-1,3,2- dioxaborolan-2-yl)aniline was obtained as a viscous yellow oil (380 mg, 39.8%). LC-MS (Method 4): Rt = 6.34, m/z = 307.9 [M+H] + . 1 H NMR (500 MHz, DMSO-d 6 ): δ 0.21 - 0.26 (m, 2H), 0.42 - 0.48 (m, 2H), 1.05 - 1.13 (m, 1H), 1.25 (s, 12H), 3.05 (t, J = 6.23 Hz, 2H), 5.67 (t, J = 5.61 Hz, 1H), 6.72 (d, J = 8.59 Hz, 1H), 7.41 (d, J = 8.26 Hz, 1H), 7.44 (s, 1H). [0773] N-[2-chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)p henyl]-N-(cyclopropylmethyl) acetamide (I-T2310) [0774] Following Scheme 1 – Step 3a: N-[2-chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)phenyl]-N-(cyclopropylmethyl)acetamide was obtained as a white solid (429 mg, 87%). LC-MS (Method 8): Rt = 1.44, m/z = 350.25 [M+H] + , 1 H NMR (500 MHz, DMSO-d 6 ): δ - 0.06 - 0.03 (m, 2H), 0.32 - 0.38 (m, 2H), 0.80 - 0.91 (m, 1H), 1.31 (s, 12H), 1.67 (s, 3H), 3.17 (dd, J = 14.08, 7.2 Hz, 1H), 3.7 (dd, J = 14.01, 7.15 Hz, 1H), 7.54 (d, J = 7.85 Hz, 1H), 7.68 - 7.72 (m, 1H), 7.78 (s, 1H). Second Exemplary Synthesis of a Compound of Formula V-b3 [0775] 4-Bromo-2-chloro-N-(2,2-difluoroethyl)aniline (I-T2427) [0776] Following Scheme 1 – Step 2b: purification by fcc (0-5 % EtOAc in cyclohexane) afforded 4- bromo-2-chloro-N-(2,2-difluoroethyl)aniline (174 mg, 67%). LC-MS (Method 3): Rt = 5.19 min, m/z = 271.72 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 3.5 – 3.68 (m, 2H), 5.84 (t, J = 6.36 Hz, 1H), 6.1 (tt, J = 55.7, 4 Hz, 1H), 6.83 (d, J = 8.8 Hz, 1H), 7.28 (dd, J = 8.8, 2.3 Hz, 1H), 7.46 (d, J = 2.3 Hz, 1H). [0777] N-(4-Bromo-2-chloro-phenyl)-N-(2,2-difluoroethyl)acetamide (I-T2428) [0778] Following Scheme 1 – Step 3a: purification by fcc (hexane:diethyl ether 1:1) afforded N-(4- bromo-2-chloro-phenyl)-N-(2,2-difluoroethyl)acetamide (1.4 g, 84%) as an oil. LC-MS (Method 8): Rt = 1.14 min, m/z = 313.87 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.73 (s, 3H), 3.53 – 3.74 (m, 1H), 4.09 – 4.30 (m, 1H), 6.15 (tt, J = 55.6, 4.0 Hz, 1H), 7.49 (d, J = 8.5 Hz, 1H), 7.69 (dd, J = 8.48, 2.3 Hz, 1H), 7.96 (d, J = 2.2 Hz, 1H). [0779] N-[2-Chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)p henyl]-N-(2,2- difluoroethyl)acetamide (I-T2429) [0780] To a solution of 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborola n-2-yl)-1,3,2- dioxaborolane (183 mg, 0.720 mmol) and N-(4-bromo-2-chloro-phenyl)-N-(2,2-difluoroethyl) acetamide (150 mg, 0.480 mmol) in dioxane (3.75 mL), was added CH 3 COOK (141 mg, 1.44 mmol, dried). The RM was degassed with argon for 5 min and Pd(dppf)Cl 2 xDCM (19.6 mg, 0.024 mmol) was added. The RM was stirred at 80 °C overnight, filtered over celite and the filtrate evaporated. The resulting residue was dissolved in EtOAc, washed with NaHCO 3 (3x) and brine, the organic layer was dried and evaporated to afford a brown crude. Purification by fcc (0-50% diethyl ether in hexane) afforded N-[2-chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)p henyl]-N-(2,2- difluoroethyl)acetamide (107 mg) as an oil that crystallizes on standing. The sample was triturated with cold pentane. Pentane was decanted and the residual solid was dried to afford N-[2-chloro-4- (4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]-N-(2,2- difluoroethyl)acetamide (80 mg, 42%). LC-MS (Method 8): Rt = 1.34 min, m/z = 360.11 [M+H] + . 1 H NMR (300 MHz, DMSO-d 6 ): δ 1.3 (s, 12H), 1.72 (s, 3H), 3.54 – 3.73 (m, 1H), 4.12 – 4.33 (m, 1H), 6.15 (tt, J = 55.6, 4.0 Hz, 1H), 7.54 (d, J = 7.7 Hz, 1H), 7.70 (dd, J = 7.7, 1.24 Hz, 1H), 7.76 – 7.79 (m, 1H). Example 2. Cell viability in MV-4-11 cells [0781] Approximately 10,000 MV-4-11 cells per well of a 96-well plate were treated with DMSO (no drug control) or each compound in Tables E1 and E2 in an 8-point dose response for 4 days. Cell viability was measured using CellTiter Glo2. The IC50 of each compound was determined using CDD Vault. Exemplary results are shown in Table E1. Example 3. NAMPT Activity Assay [0782] The NAMPT Activity Assay (Abcam # ab221819) was performed following manufacturers two-step reaction protocol. Briefly, compounds were added to a clear 96-well plate using the HP D300e Digital Dispenser. Purified NAMPT was added to all wells except the No NAMPT controls. Reaction Mix #1 was added to each well and the plate was incubated at 30*C for 1 hr followed by the addition of Reaction Mix #2. Absorbance was measured at OD450nm using a multimode plate reader. Readings were recorded for 5 minutes and percent inhibition was calculated using: (1- (OD450nm compound/ OD450nm no drug)) * 100. Exemplary results are shown in Table E2. Table E1. MV-4-11 Cell Viability Table E2. NAMPT Inhibition REFERENCES 1. American Cancer Society.2017. Cancer Facts & Figures, 2017. Atlanta: American Cancer Society. 2. Stewart BW, Wild CP, editors. World cancer report 2014 Lyon: International Agency for Research on Cancer; 2014. 3. Taniguchi H, Moriya C, Igarashi H, Saitoh A, Yamamoto H, Adachi Y, Imai K.2016. Cancer stem cells in human gastrointestinal cancer. Cancer Sci 107: 1556–1562. 4. Seyfried T and Huysentruyt LC.2013. On the Origin of Cancer Metastasis. Crit Rev Oncog. 18(1-2): 43–73. 5. Bonnet D and Dick JE.1997. 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See also: (I) WO 97/48696 for involvement of NAMPT in the treatment of cancer, (II) WO 97/48397 for involvement of NAMPT in immunosuppression, (III) WO 2003/80054 for involvement of NAMPT for the treatment of diseases involving angiogenesis, (IV) WO 2008/025857 for involvement of NAMPT for the treatment of rheumatoid arthritis and septic shock, and (v) WO 2009/109610 for involvement of NAMPT for the prophylaxis and treatment of ischemia. 18. Moschen, A, et al. J Immunol.2007, 178, 1748. 19. Zhang, Y, et al. Mol Med Rep.2019, 19, 400. 20. Moreno-Vinasco L, Quijada H, Sammani S, et al.2014. Nicotinamide Phosphoribosyltransferase Inhibitor Is a Novel Therapeutic Candidate in Murine Models of Inflammatory Lung Injury. Am. J. Respir. Cell. Mol. Biol.51(2), 223-8. 21. Wu GC, Liao WI, Wu SY, et al.2017. Targeting of nicotinamide phosphoribosyltransferase enzymatic activity ameliorates lung damage induced by ischemia/reperfusion in rats. Respir. Res.18(1), 71. 22. 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Travelli C, Consonni FM, Sangaletti S, et al.2019. Nicotinamide Phosphoribosyltransferase Acts as a Metabolic Gate for Mobilization of Myeloid-Derived Suppressor Cells. Cancer Res. 79(8), 1938-51. 28. Chen CX, Huang J, Tu GQ, et al.2017. NAMPT inhibitor protects ischemic neuronal injury in rat brain via anti-neuroinflammation. Neuroscience.356, 193-206. 29. Wang P, Xu TY, Guan YF, et al.2011. Nicotinamide phosphoribosyltransferase protects against ischemic stroke through SIRT1-dependent adenosine monophosphate-activated kinase pathway. Ann. Neurol.69(2), 360-74. 30. Esposito E, Impellizzeri D, Mazzon E, et al.2012. The NAMPT inhibitor FK866 reverts the damage in spinal cord injury. J. Neuroinflammation.9, 66. 31. Romacho T, Azcutia V, Vazquez-Bella M, et al.2009. Extracellular PBEF/NAMPT/visfatin activates pro-inflammatory signalling in human vascular smooth muscle cells through nicotinamide phosphoribosyltransferase activity. Diabetologia.52, 2455-63. 32. Vallejo S, Romacho T, Angulo J, et al.2011. Visfatin impairs endothelium-dependent relaxation in rat and human mesenteric microvessels through nicotinamide phosphoribosyltransferase activity. PloS One.6(11), e27299. 33. Bermudez B, Dahl TB, Medina I, et al.2017. Leukocyte overexpression of intracellular NAMPT attenuates atherosclerosis by regulating PPARg-dependent monocyte differentiation and function. Arterioscler. Thromb. Vasc. Biol.37, 1157-67. 34. Stromsdorfer KL, Yamaguchi S, Yoon MJ, et al.2016. NAMPT-mediated NAD + biosynthesis in adipocytes regulates adipose tissue function and multi-organ insulin sensitivity in mice. Cell Rep.16(7), 1851-60. 35. Benito-Martin A, Ucero AC, Izquierdo MC, et al.2014. Endogenous NAMPT dampens chemokine expression and apoptotic responses in stressed tubular cells. Biochim. Biophys. Acta.1842(2), 293-303. 36. Chen Y, Liang Y, Hu T, et al. (2017). Endogenous Nampt upregulation is associated with diabetic nephropathy inflammatory-fibrosis through the NF-kB p65 and Sirt1 pathway; NMN alleviates diabetic nephropathy inflammatory-fibrosis by inhibiting endogenous Nampt. Exp. Ther. Med.14(5), 4181-4193. EQUIVALENTS AND SCOPE [0783] In the claims, articles such as “a,” “an,” and “the” may mean one or more than one unless indicated to the contrary or otherwise evident from the context. Claims or descriptions that include “or” between one or more members of a group are considered satisfied if one, more than one, or all of the group members are present in, employed in, or otherwise relevant to a given product or process unless indicated to the contrary or otherwise evident from the context. The invention includes embodiments in which exactly one member of the group is present in, employed in, or otherwise relevant to a given product or process. The invention includes embodiments in which more than one, or all of the group members are present in, employed in, or otherwise relevant to a given product or process. [0784] Furthermore, the invention encompasses all variations, combinations, and permutations in which one or more limitations, elements, clauses, and descriptive terms from one or more of the listed claims is introduced into another claim. For example, any claim that is dependent on another claim can be modified to include one or more limitations found in any other claim that is dependent on the same base claim. Where elements are presented as lists, e.g., in Markush group format, each subgroup of the elements is also disclosed, and any element(s) can be removed from the group. It should it be understood that, in general, where the invention, or aspects of the invention, is/are referred to as comprising particular elements and/or features, certain embodiments of the invention or aspects of the invention consist, or consist essentially of, such elements and/or features. For purposes of simplicity, those embodiments have not been specifically set forth in haec verba herein. It is also noted that the terms “comprising” and “containing” are intended to be open and permits the inclusion of additional elements or steps. Where ranges are given, endpoints are included. Furthermore, unless otherwise indicated or otherwise evident from the context and understanding of one of ordinary skill in the art, values that are expressed as ranges can assume any specific value or sub-range within the stated ranges in different embodiments of the invention, to the tenth of the unit of the lower limit of the range, unless the context clearly dictates otherwise. [0785] This application refers to various issued patents, published patent applications, journal articles, and other publications, all of which are incorporated herein by reference. If there is a conflict between any of the incorporated references and the instant specification, the specification shall control. In addition, any particular embodiment of the present invention that falls within the prior art may be explicitly excluded from any one or more of the claims. Because such embodiments are deemed to be known to one of ordinary skill in the art, they may be excluded even if the exclusion is not set forth explicitly herein. Any particular embodiment of the invention can be excluded from any claim, for any reason, whether or not related to the existence of prior art.
[0786] Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation many equivalents to the specific embodiments described herein. The scope of the present embodiments described herein is not intended to be limited to the above Description, but rather is as set forth in the appended claims. Those of ordinary skill in the art will appreciate that various changes and modifications to this description may be made without departing from the spirit or scope of the present invention, as defined in the following claims.