Title:
NANO-APTAMER FOR MULTI-SPECIFIC ANTIBODY DELIVERY, APPLICATION THEREOF AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/027828
Kind Code:
A1
Abstract:
The present invention relates to a nano-aptamer for multi-specific antibody delivery, an application thereof and a construction method therefor. The nano-aptamer is formed by connecting a nanocarrier to an anti-Fc segment antibody or an anti-Fc segment antibody fragment portion by means of a chemical bond; a Fab domain of the anti-Fc segment antibody or the anti-Fc segment antibody fragment can non-covalently bind to an Fc domain of a delivered specific antibody; the delivered specific antibody has the same species origin as the Fc segment recognized by the anti-Fc segment antibody or the anti-Fc segment antibody fragment. The nano-aptamer can quickly, efficiently, and controllably bind to multiple types of antibodies, achieving the "multivalence" and "multi-specificity" of antibodies. For the first time, said type of constructed nanobody delivery platform is creatively applied to the preparation of an immunotherapeutic drug or therapeutic drug for tumors or autoimmune diseases, and can significantly improve the effect of immunotherapy.
Inventors:
SHEN SONG (CN)
WANG JUN (CN)
JIANG CHENGTAO (CN)
XU CONGFEI (CN)
YANG XIANZHU (CN)
WANG JUN (CN)
JIANG CHENGTAO (CN)
XU CONGFEI (CN)
YANG XIANZHU (CN)
Application Number:
PCT/CN2020/122360
Publication Date:
February 10, 2022
Filing Date:
October 21, 2020
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
International Classes:
A61K47/54; A61K39/395; A61P35/00; A61P37/02
Foreign References:
| CN107532149A | 2018-01-02 |
Other References:
VLLASALIU DRITON, CAMERON ALEXANDER, MARTIN GARNETT, MIKE EATON, SNOW STOLNIK: "Fc-mediated Transport of Nanoparticles Across Airway Epithelial Cell Layers", JOURNAL OF CONTROLLED RELEASE, vol. 158, 20 December 2011 (2011-12-20), pages 479 - 486, XP055893107, DOI: 10.1016/j.jconrel.2011.12.009
JOSE M. GUISAN: "Methods in Molecular Biology", vol. 1051, 31 December 2013, SPRINGER, ISBN: 978-1-62703-550-7, article POLO ESTER, PUERTAS SARA, MOROS MARÍA, BATALLA PILAR, GUISÁN JOSÉ M., DE LA FUENTE JESÚS M., GRAZÚ VALERIA: "Chapter 11: Tips for the Functionalization of Nanoparticles with Antibodies", pages: 149 - 163, XP009534036, DOI: 10.1007/978-1-62703-550-7_11
WANG DANGGE, WANG TINGTING, YU HAIJUN, FENG BING, ZHOU LEI, ZHOU FANGYUAN, HOU, ZHANG HANWU, LUO MIN, LI YAPING: "Engineering nanoparticles to locally activate T cells in the tumor microenvironment", SCIENCE IMMUNOLOGY, vol. 4, no. 37, 5 July 2019 (2019-07-05), US , pages eaau6584, 1 - 13, XP009534039, ISSN: 2470-9468, DOI: 10.1126/sciimmunol.aau6584
PRIDGEN ERIC M., ALEXIS FRANK, KUO TIMOTHY T., LEVY-NISSENBAUM ETGAR, KARNIK ROHIT, BLUMBERG RICHARD S., LANGER ROBERT, FAROKHZAD : "Transepithelial Transport of Fc-Targeted Nanoparticles by the Neonatal Fc Receptor for Oral Delivery", SCIENCE TRANSLATIONAL MEDICINE, vol. 5, no. 213, 27 November 2013 (2013-11-27), XP055895125, ISSN: 1946-6234, DOI: 10.1126/scitranslmed.3007049
SOCKOLOSKY JONATHAN T.; SZOKA FRANCIS C.: "The neonatal Fc receptor, FcRn, as a target for drug delivery and therapy", ADVANCED DRUG DELIVERY REVIEWS, vol. 91, 19 February 2015 (2015-02-19), Amsterdam , NL , pages 109 - 124, XP029265307, ISSN: 0169-409X, DOI: 10.1016/j.addr.2015.02.005
JOSE M. GUISAN: "Methods in Molecular Biology", vol. 1051, 31 December 2013, SPRINGER, ISBN: 978-1-62703-550-7, article POLO ESTER, PUERTAS SARA, MOROS MARÍA, BATALLA PILAR, GUISÁN JOSÉ M., DE LA FUENTE JESÚS M., GRAZÚ VALERIA: "Chapter 11: Tips for the Functionalization of Nanoparticles with Antibodies", pages: 149 - 163, XP009534036, DOI: 10.1007/978-1-62703-550-7_11
WANG DANGGE, WANG TINGTING, YU HAIJUN, FENG BING, ZHOU LEI, ZHOU FANGYUAN, HOU, ZHANG HANWU, LUO MIN, LI YAPING: "Engineering nanoparticles to locally activate T cells in the tumor microenvironment", SCIENCE IMMUNOLOGY, vol. 4, no. 37, 5 July 2019 (2019-07-05), US , pages eaau6584, 1 - 13, XP009534039, ISSN: 2470-9468, DOI: 10.1126/sciimmunol.aau6584
PRIDGEN ERIC M., ALEXIS FRANK, KUO TIMOTHY T., LEVY-NISSENBAUM ETGAR, KARNIK ROHIT, BLUMBERG RICHARD S., LANGER ROBERT, FAROKHZAD : "Transepithelial Transport of Fc-Targeted Nanoparticles by the Neonatal Fc Receptor for Oral Delivery", SCIENCE TRANSLATIONAL MEDICINE, vol. 5, no. 213, 27 November 2013 (2013-11-27), XP055895125, ISSN: 1946-6234, DOI: 10.1126/scitranslmed.3007049
SOCKOLOSKY JONATHAN T.; SZOKA FRANCIS C.: "The neonatal Fc receptor, FcRn, as a target for drug delivery and therapy", ADVANCED DRUG DELIVERY REVIEWS, vol. 91, 19 February 2015 (2015-02-19), Amsterdam , NL , pages 109 - 124, XP029265307, ISSN: 0169-409X, DOI: 10.1016/j.addr.2015.02.005
Attorney, Agent or Firm:
GUANGZHOU PRIME IP LAW OFFICE (CN)
Download PDF: