LI, Chun (6807 Spanish Bay Court, Missouri City, TX, 77459, US)
LI, Chun (6807 Spanish Bay Court, Missouri City, TX, 77459, US)
| Claims LA product comprising any feature described herein, either individually or in combination with any feature, in any configuration. 2.A composition comprising an alpha-galactosylceramide conjugated nanoparticle. 3.A composition comprising β-aminoethoxy-ό-deoxy-alpha-galactosylceramide. 4.A composition of claim 2 wherein the nanoparticle comprises polylactic acid, poly(glycolic acid), polycaprolactone, and other polyester species; polyothoesters species; polyanhydrides species and polyphosphazene species, as well as any copolymers composed by 2 or more than 2 species of polymers as mentioned. 5.A process of making a conjugated nanoparticle alpha-galactosylceramide formulation wherein alpha-galactosylceramide or its analogs are chemically conjugated to the surface of nanoparticles through amide linkage or other chemical linkages. 6.A pharmaceutical composition comprising a conjugated nanoparticle alpha-galactosylceramide. 7.A method of preventing anergy of NKT cells comprising the step of administering a therapeutically effective amount of a conjugated nanoparticle formulated with alpha-galactosylceramide to a patient in need thereof. 8.A method of preventing or treating cancer, chronical viral disease, chronic bacteria infections, autoimmune diseases, asthma, allergy, diabetes, atherosclerosis, and other chronic disease conditions, comprising administering a therapeutically effect amount of a conjugated nanoparticle alpha-galactosylceramide in combination with a protein/peptide antigen to a patient in need thereof. 9.An adjuvant for protein/peptide antigen comprising a conjugated alpha-galactosylceramide nanoparticle. |
NANOPARTICLE FORMULATED GLYCOLIPID ANTIGENS FOR
IMMUNOTHERAPY
[1] FIELD OF THE INVENTION
[2] The present invention relates to a novel nanoparticle formulated glycolipid antigen which has a strong activity to trigger the immune system to release anticancer and antiviral cytokines. In contrast to previous invented glycolipid antigens, such novel nanoparticle formulation does not cause the anergy (non-responsiveness after stimulation) of the immune system, and can be used repeatedly to treat long term illness such as cancer and chronic viral diseases.
[3] BACKGROUND OF THE INVENTION
[4] Alpha-galactosylceraniide (KRN7000), a potent immunotherapeutic. alpha-galactosylceramide ( also named as KRN7000), is a marine-sponge derived glycosphingolipid (US patent 5,780,441). It is a super-agonist antigen for NKT cells, limits melanoma metastasis and inhibits the replication of hepatitis B virus in mouse models through a ThI cytokine (IFN-γ) mediated mechanism. In several clinical trials involving cancer and hepatitis C patients, alpha-galactosylceramide has been demonstrated to be safe, and to induce strong ThI cytokine response.
[5] Natural Killer T cells are a unique subset of lymphocytes that have markers and functions of T cells and NK cells. NKT cells are activated within 2 hr of antigen (alpha-galactosylceramide) stimulation, and produce large amount of ThI and Th2 cytokines. In addition to secreting cytokines, NKT cells play extremely important role in initiating the cell-to-cell contact and crosstalk between several types of immune cells, including dendritic cells, macrophages, CD4 T cells and CD8 T cells. In contrast to conventional T cells which recognize peptide antigens, NKT cells recognize lipid antigens presented by the non-polymorphic MHC-like molecule CDId. [6] Soluble alpha-galactosylceramide induces anergy of NKT cells. A major problem for alpha-galactosylceramide is, it causes NKT non-responsiveness (anergy) after one dose of treatment, because alpha-galactosylceramide can be presented by CDId expressing B cells in the peripheral blood (Figure IA), and stimulate the NKT cells without proper co-stimulatory molecules.
[7] To overcome the anergy induced by soluble alpha-galactosylceramide, Dhodapkar and Steinmann have developed a cell therapy approach (Figure IB), by intravenously injecting alpha-galactosylceramide pulsed, ex vivo generated dendritic cells from patients' peripheral blood mononuclear cells. The cell therapy method avoided the NKT anergy mechanism, and showed potent efficacy in cancer patients in eliciting tumor antigen-specific CD8 responses. However, cell therapy is expensive, and impracticable for virus infected patients since their tissues are excluded from GMP processing. Hence, new methods are needed to stimulate NKT cells in vivo repeatedly and effectively. [8] Nanoparticle formulated alpha-galactosylceramide overcomes NKT anergy. Based on the above progress, we have formed the hypothesis that alpha-galactosylceramide, packed in nanoparticles, will be preferentially taken up by dendritic cells and released into lysosomes (Figure 1C). Alpha-galactosylceramide is loaded to the antigen presenting molecule, CDId (a non-MHC antigen presenting molecule), in the lysosome, and recycled to cell surface, which activates NKT cells. We have invented a technology through chemical synthesis, to conjugate alpha-galactosylceramide to the surface of polymer based nanoparticles.
[9] Rationale to use surface conjugating of alpha-galactosylceramide, instead of encapsulating approach. There are two approaches for our consideration to load alpha-galactosylceramide in nanoparticles: 1) surface coating, which means we synthesize nanoparticles first, and conjugate alpha-galactosylceramide to the surface of nanoparticles; 2) direct encapsulating approach, which means to mix alpha-galactosylceramide and polymer together, and form the nanoparticles with alpha-galactosylceramide evenly distributed inside the nanoparticles. We have decided to use the former approach, due to the biological behavior of NKT cells, the cell type which respond to alpha-galactosylceramide drug.
[10] As demonstrated in Figure 2, the stimulation of NKT cells includes the expansion, contraction, and memory phases. In the expansion phase, the NKT cells produce anti-tumor and anti-viral cytokines. In contraction phase and memory phase, most NKT cells die, while 5 to 10% of "memory" NKT cells begin to "rest" and re-program themselves for the next stimulation. Thus a continuous release of alpha-galactosylceramide will not cause effective stimulation of NKT cells, instead, continuous release of alpha-galactosylceramide has been shown to cause the non-responsiveness (anergy) of NKT cells. In view of the above mechanism, we designed the method of surface coating, which causes rapid release of alpha-galactosylceramide upon being phagocytosed by dendritic cells and macrophages, but not consistent release of alpha-galactosylceramide to the serum. In contrast, it is known that encapsulating method is suitable for long term, constant release of encapsulated drugs. Consistent release of encapsulated drugs can last for up to 10 days after intravenous injection of encapsulating nanoparticles, which accumulate in liver and spleen. Thus, encapsulating method does not fit for the purpose of releasing NKT ligand.
[11] Rationale to choose biodegradable polymer based nanoparticles. Polymers such as PLGA has excellent biocompatibility, biodegradability, commercial availability, and previous application to deliver drugs such as proteins, peptide vaccines, and hydrophobic anticancer drugs.
[12] The acyl groups of polymers, exposed on the surface of nanoparticles, can be utilized to conjugate with amine groups. However, alpha-galactosylceramide does not contain amine groups. Since the 6-OH group of galactose is known to be dispensable for the antigenic activity of alpha-galactosylceramide, we have invented a glycolipid analog, β-aminoethoxy-β-deoxy-alpha-galactosylceramide, which contains amine group in 6-OH group of galactose. This allows the formation of amide linkage (Figure 3). To avoid antibody response to any additional protein (such as streptavidin-biotin system), we will directly conjugate alpha-galactosylceramide to nanopolymer materials. Both alpha-galactosylceramide and polymers are not antigenic in mouse and human, and the chemical conjugates of polymers and alpha-galactosylceramide do not create immuno-reactive epitopes.
[13] SUMMARY Q F THE INVENTION
[14] The present invention relates to a nanoparticle formulated glycolipid antigen, containing 2 components:
[15] 1). Nanoparticles at size of 10 to 2000 nm, composed by biodegradable polymers, such as, poly (lactic acid), poly(glycolic acid), polycaprolactone, and other polyester species; polyothoesters species; polyanhydrides species and polyphosphazene species, as well as any copolymers composed by 2 or more than 2 species of polymers as mentioned above.
[16] 2) 6-aminoethoxy-6-deoxy-alpha-galactosylceramide
[17] The key technology applied to this invention is, component 2 is chemically conjugated to the surface of component 1, through amide linkage (Figure 3)
[18] The object of this invention is to provide a novel surface conjugated formulation to a glycolipid antigen, which can stimulate immune system repeatedly in long term treatment of cancer and chronic viral infection status.
[19] The present inventors found the glycolipid conjugated to surface of nanoparticles can repeatedly stimulate NKT cells to produce IFN-γ, a cytokine with potent anticancer and antiviral activities. This novel drug has been found to have anticancer effect in a mouse model of tumor metastasis.
[20] BEST MODE OF CARRYING OUT THE INVENTION
[21] Preparation of nanoparticles, with poly (lactie-co-gly colic acid) as example
(abbreviated as PLGA). The nanospheres will be prepared using nanoprecipitation method in the presence of the stabilizing copolymer. The PLGA, from Absorbable Polymers International (Pelham, AL, USA), will be dissolved in acetone (0.5% w/v). The polymer solution will be added drop-wise to an aqueous solution containing poloxamine 904 (BASF Wyandotte Corporation, Parsippany, NJ, USA). The mixture will be stirring at room temperature until the organic solvent is completely dried. The resulting nanoparticle dispersions will be passed through a 1-μm filter, and purified by centrifugation. Nanoparticles will be re-dispersed in water, flash-frozen in liquid nitrogen and subject to lyophilization. The dry powder formulation will be stored in - 20 °C. This protocol will provide nanoparticles with the diameter of about 50 nm to 2000 nm, depending on the molecular weight of PLGA, stirring rate, and the concentration of surfactant.
[22] Preparation of ό-aminoethoxy-ό-deoxy-alpha-galactosylceramide, which contains amine group for direct conjugating to PLGA nanoparticles (Figures 4&5).
[23] Conjugation of ό-aminoethoxy-ό-deoxy-alpha-galactosylceramide to PLGA nanoparticles. 6-aminoethoxy-6-deoxy-alpha-galactosylceramide will be conjugated to PLGA particles at 1% and 10% (w/w ratio).
[24] EXPERIMENTAL EXAMPLE 1 : IMMUNSTIMULATORY ACTIVITY OF THE DRUG OF THE PRESENT INVENTION
[25] In vivo stimulation of NKT cells by nanoparticle formulated αGalCer:
Nanoparticle containing 1 μg alpha-galactosylceramide was injected to C57BL6 mice, with non-conjugated nanoparticles as negative control. In parallel, we studied soluble form of alpha-galactosylceramide.
[26] The repeated in vivo NKT cell stimulation: C57/BL6 mice were purchased from the Jackson Laboratory (Bar Arbor, ME) and housed in M.D. Anderson Cancer Center animal facilities under standard pathogen free conditions abiding institutional guidelines. 6 weeks old C57BL/6 mice were used for all experiments. 3 mice per group were used for each experiment. 1 μg alpha-galactosylceramide, or nanoparticle formulated alpha-galactosylceramide in 200 μL PBS was intravenously injected (through tail vein) to each mouse. Mice were treated every 10 days for 3 times. 200 μL of PBS/1 %DMSO was used as control.
[27] Measurement of IFN-y secretion after each stimulation: Mice were bled at 24 h after each drug treatment. Serum IFN-γ was measured by ELISA using a kit from BD Biosciences (San Jose, CA).
[28] Results: Nanoparticle overcomes the anergy of NKT cells: Similarly to alpha-galactosylceramide, the first injection of nanoparticle formulated alpha-galactosylceramide elicited cytokine release, peaked at 24-48 h post injection, as measured by serum IFN-γ concentration. As shown in Table 1, nanoparticle formulated alpha-galactosylceramide induced IFN-γ secretion after each stimulation. In contrast, soluble alpha-galactosylceramide caused IFN-γ secretion only at the first treatment and failed to induce upon subsequent stimulations. Thus our new formulation of alpha-galactosylceramide can repeatedly stimulate NKT cells and induce IFN-γ production without leading to anergy.
Table 1. αGalCer-nanoparticles repeatedly activate NKT cells to produce IFN-γ (pg/mL).
[34] EXPERIMENTAL EXAMPLE 2: ANTITUMOR ACTIVITY OF THE DRUG OF THE PRESENT INVENTION
[35] Anti-metastasis effect. The B16F10 melanoma lung metastasis tumor model was employed to evaluate the anticancer function of the present invention. 48 hours after drugjreatment, 1 x 10 5 Bl 6F10 melanoma cells were injected intravenously through tail vein. 14 days after tumor injection, the mice were sacrificed and the tumor nodes formed through metastasis (primary end point) were counted in mouse lung. [36] Results: As showed in Figure 6, the present invention has significant anticancer effect.
[37] BRIEF DESCRIPTION OF THE DRAWINGS
[38] The structure and the technical means adopted by the present invention to achieve t he above and other objects can be best understood by referring to the following detailed description of the preferred embodiments and the accompanying drawings, wherein
[39] Fig. 1 is a perspective view of 3 forms of alpha-galactosylceramide, according to a first embodiment of the present invention;
[40] Fig. 2 is a perspective view of the expansion, contraction, and memory phases of NKT cells after alpha-galactosylceramide according to the present invention, explaining why the alpha-galactosylceramide must be conjugated to the surface of nanoparticles, instead of being encapsulated inside, according to conventional wisdom. [41] Fig.3 is a perspective view of the chemical linkage binding alpha-galactosyceramide to the surface of nanoparticles. [42] Fig.4 is aperspective view of a newly invented structure, ό-aminoethoxy-ό-deoxy-alpha-galactosylceramide, a novel glycolipid analog of alpha-galactosylceramide, which contains amine group for chemical conjugation to the surface of nanoparticles. [43] Fig.5 is a perspective view of the chemical synthesis route for producing
6-aminoethoxy-6-deoxy-alpha-galactosylceramide, a novel glycolipid analog of alpha-galactosylceramide.
[44] Fig.6 is a perspective view of an example of the anticancer effect of this invention.
[45] DETAILED DESCRIPTION OFTHE PREFERRED EMBODIMENTS
[46] The present invention will now be described with some preferred embodiments thereof. For the purpose of easy to understand, elements that are the same in the preferred embodiments are denoted by the same reference numerals.
[47] Please refer to Fig. 1.
[48] A. Soluble form of αGalCer is mainly presented by B cells, which stimulates NKT cells without co-stimulatory signals, and cause NKT anergy after once drug injection. [49] B. αGalCer loaded dendritic cells stimulate NKT cells without causing anergy, but generation of dendritic cells for cell therapy is a tedious and expensive procedure. [50] C. Nanopartile formulated αGalCer is designed to be preferentially taken up by dendritic cells. The αGalCer will be released in the lysosome and recycled to cell surface, which stimulate NKT cells without causing anergy.
[51] Fig. 2 shows the stimulation of NKT cells include expansion, contraction, and memory phase. αGalCer can not be delivered in a continuously released form, because continuous exposure of NKT cells to αGalCer will cause non-responsiveness (anergy).
[52] Fig. 3 shows Component 2 (containing amine group) is conjugated to the surface of component 1 (containing acyl group) through amide linkage.
[53] Fig.4 shows ό-aminoethoxy-ό-deoxy-alpha-galactosylceraniide, a novel structure we have invented, which with structural similarity to alpha-galactosylceramide (KKN7000), but can be directly conjugated to PLGA nanoparticles through its amide group (red color).
[54] Fig. 5 shows the chemical synthesis route we have invented for producing ό-aminoethoxy-β-deoxy-alpha-galactosylceramide.
[55] Fig.6 shows anticancer effect of nanoparticle-formulated alpha-galactosylceramide. The Bl 6F10 melanoma lung metastasis tumor model was used to test the tumor preventing activity of nanoparticle formulated alpha-galactosylceramide drugs in mice. 48 hours after nanoparticle drug treatment, 1 x 10 5 Bl 6F10 melanoma cells were injected intravenously through tail vein. 14 days after tumor injection, the mice were sacrificed and the tumor nodes formed through metastasis were counted in mouse lung, after being fixed by Fekete's solution.
[56] PBS: control mice treated by PBS.
[57] αGalCer: 2 μg soluble form of αGalCer.
[58] αGalCer 200 nm: 2 μg αGalCer bound to 200 nm size of polylactic acid nanoparticles.
[59] αGalCer 2000 nm: 2 μg αGalCer bound to 2000 nm size of polylactic acid nanoparticles.
[60] Each group contained 5 mice (X ^- H^ *)•