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Title:
NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT CONSISTING OF EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE
Document Type and Number:
WIPO Patent Application WO/2012/008841
Kind Code:
A1
Abstract:
The present development brings a technical innovation for manipulating natural-origin enzymatic elements developed by using enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where there is a synthesis reaction creating expressive conditions for bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces. It is a process of using enzymes for bioremediation in a substrate, which due to its basic substances' characteristics, presents an inexpressive toxicity, high floatability degree and the ability to absorb contaminant in a ratio weight/weight in the range of 2.000% to 2.500%, and in the ratio volume/volume in the range of 100% to 200%. In the present process we present a technological innovation for manipulation of natural-origin enzymatic elements, using them in a specific way and for a specific purpose, which due to these characteristics, when in contact with liquid carbon substances provide a considerable reduction of the biodegradability time and the transformation of the bioremediator/polluent mix when collected in fully organic compost.

Inventors:
ALVES PEREIRA, Luiz Antonio (Rua Maria Amanda Cruz Pires, 13Maria Paula, São Gonçalo - RJ, BR)
ROBERTO, Lazaro Sebastiao (Rua Padre Flavio Silva, 951Olinda, Uberaba - MG, BR)
Application Number:
NO2010/000284
Publication Date:
January 19, 2012
Filing Date:
July 14, 2010
Export Citation:
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Assignee:
ALVES PEREIRA, Luiz Antonio (Rua Maria Amanda Cruz Pires, 13Maria Paula, São Gonçalo - RJ, BR)
ELLINGSEN, Hans Falnes (Avenida Sernambetiba, 5300 Bloco 3, Apto 80, Barra da Tijuca RlO DE JANEIRO - RJ, BR)
WALVAG, Kolfinn Roger (Rua Visconde do Uruguai 27, Ponta da Areia NITEROI - RJ, BR)
ROBERTO, Lazaro Sebastiao (Rua Padre Flavio Silva, 951Olinda, Uberaba - MG, BR)
International Classes:
C02F1/68; B09C1/10; C02F3/34; E02B15/00
Attorney, Agent or Firm:
ZACCO NORWAY AS (P.O. Box 2003 Vika, Oslo, N-0125, NO)
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Claims:
CLAIMS

The present request for an invention patent refers to a technical innovation for manipulating natural-origin enzymatic elements developed by using enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where there is a synthesis reaction creating expressive conditions for bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces.

Claim 1 , consists of a process for mixing the substrate where the extracts of oleaginous vegetable sources are blended into a mixture of isomers, causing a reaction of binding and expansion and the emergence of a polymeric substrate;

Claim 2, consists of addition in predefined proportions of natural origin polymeric biomolecules in specific doses of: Oxdoreductases 125g, Hydroxylases 100g, Ligases 80, 5g e Isomerases 100g on a culture base per m3;

Claim 3, consists of the final processing of the physical structural modification of the product which resulted from the enzymatic addition to the polymeric substrate.

Description:
"NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE

APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT

CONSISTING OF EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE".

SPECIFICATION

The present request for an invention patent refers to an innovative technology developed from the use of enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where a synthesis reaction creating expressive conditions for bioremediation of carbon molecules takes place.

The necessity of the present development becomes evident when we face the reality of a permanent environmental threat: pollution by liquid substances containing carbon molecules, occasioned by human failure or break-down of systems or equipment.

When there is an accident, the methodology presently applied for the absorption of liquid carbon substances from the environment is using absorbing materials. However, due to its non-degradability, these materials have the inconvenience of substantially increasing the residue volume, sometimes causing worse harm to the environment. In some cases (like in the water), due to higher density and/or affinity with the aquatic environment, it makes the oil sink. In other cases, the lixiviation processes (the oil initially absorbed comes loose), make the pollutant return to the environment, and on land, it impedes the mechanic action of the oils in the gas exchange processes between free O 2 and the epicyclic substrate.

The utilization of natural-origin polymeric enzymes as substrate in an environment consisting of extracts of oleaginous vegetable sources, added to a mixture of isomer, has considerable advantages in relation to the above mentioned products for the removal of oily substances, because they are able to quickly transform the substances into a totally organic compound by action of the mentioned enzymes in this specific substrate (which, due to its qualities, foment an electrovalense action with the carbon molecules, adding it to the environment, which immediately puts an end to the aggression against the ecosystem). The density is lower than water (even when associated to the oils) and chemically apolar (suffering total rejection by the aquatic environment) granting permanent floatability, impeding the reintroduction of the polluting element into the aquatic environment, and when on land, it impedes the mechanical action of the oils in the gas exchange processes between free 0 2 and the epicyclic environment when the polluting element is still in a contaminant stage.

The production technology of the present invention has three distinct phases, which are:

1) - Mixing process of substrate - This phase proceeds from the mixture of extract of oleaginous vegetable sources with a mix of isomers forcing a binding and expansion reaction and the appearance of a polymeric substrate;

2) - Addition of predefined proportions of natural-origin polymeric biomolecules - Specific doses of: Oxdoreductases 12,5g, Hydroxylases 10g, Ligases 8,5g e Isomerases 10g on a culture base per m 3 ;

3) Final processing - Structural physical modification of the product resulting from the enzymatic addition to the polymeric substrate.

The object of the present request for the Invention Patent, called "NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT CONSISTING OF EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE" is an innovative enzymatic technology

introduced in a substrate of oleaginous vegetable extracts added to a mixture of isomers for the bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces, with an efficiency that is superior to existing technologies and products. Because the substrate is granulated, thus causing the addition to its environment of liquid oily contaminating substances in a ratio of weight/weight in the range of 2.000% to 2.500% and in the ratio volume/volume in the range of 100% to 200%, which, due to the substances' basic characteristics has an inexpressive toxicity degree and a high floatability degree, we have in this process come up with a technologic innovation which is a methodology of

manipulating the enzymatic elements from natural origin, using them in a specific way and for a specific purpose.




 
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