NEURAL STEM CELL HAVING INCREASED PASSAGE ABILITY, METHOD FOR PRODUCING SAID NEURAL STEM CELL HAVING INCREASED PASSAGE ABILITY, AND METHOD FOR CULTURING NEURAL STEM CELL FOR INCREASING PASSAGE ABILITY OF SAID NEURAL STEM CELL

Title:

Document Type and Number:

WIPO Patent Application WO/2014/069431

Kind Code:

A1

Abstract:

The present invention addresses the problem of providing: a neural stem cell having an increased passage ability; a method for producing the neural stem cell having an increased passage ability; and other. In one embodiment, the present invention provides a neural stem cell having an increased passage ability, said neural stem cell having the following characteristic properties: (a) a N-type calcium channel gene is knocked out or knocked down in the cell; (b) the inflow of Ca2+ through a N-type calcium channel is substantially eliminated or reduced in the cell; (c) the cell can be subcultured for at least 4 generations (preferably 15 generations); and (d) the cell can maintain an ability of being differentiated into a nerve cell even after the cell is subcultured for 4 generations (preferably 15 generations).

Inventors:

MIYAMOTO NORIMASA (JP)
ONO YUICHI (JP)
NAMIKI KANA (JP)
KASUYA YOSHITOSHI (JP)

Application Number:

PCT/JP2013/079200

Publication Date:

May 08, 2014

Filing Date:

October 29, 2013

Export Citation:

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Assignee:

EISAI R&D MAN CO LTD (JP)

International Classes:

C12N5/10; C12N5/0797; C12N15/09

Domestic Patent References:

WO2001030137A1	2001-05-03
WO2003035698A1	2003-05-01

Foreign References:

JP2003018945A	2003-01-21
US20060035373A1	2006-02-16
US20120107934A1	2012-05-03

Other References: