[0001] 11 The present invention relates to stabilized derivatives and peptidomimetics of a core amino acid sequence in β-amyloid (Aβ ) for treatment and prevention of β-amyloid-induced toxicity.

REFERENCE TO SEQUENCE LISTING

[0002] Applicant asserts that the information recorded in the form of an Annex C/ST.25 text file submitted under Rule 13Jer.1 (a). entitled UNIA_16_46_PCT_Sequence_Listing_ST25.txt, is identical to that forming part of the international application as filed. The content of the sequence listing is incorporated herein by reference in its entirety.

BACKGROUND OF THE INVENTION

[0003] Alzheimer ^' s disease (AD) is a progressive neurodegenerative disorders leading to dementia. It is a progressive neurodegenerative disease having symptoms such by memory loss, cognitive decline, and language dysfunction. AD is characterized by various pathological hallmarks including extracellular plaques composed primarily of β-amyk>id (Aβ ). intracellular neurofibrillary tangles formed from hyperphosphorylated tau, synaptic loss, local inflammation, and neurodegeneration in brain regions critical for memory processes. The onset of AD is best correlated with increased levels of Aβ . There is a substantial body of research to indicate that Aβ is one of the key triggers of the disease, leading to loss of synapses (the points of communication between nerve cells) and nerve cells (lines of communication) in regions of the brain involved with memory and cognition, both of which eventually fail in AD.

[0004] The β-amyloid is a small, toxic peptide cleaved from the amyloid precursor protein (APP). APP is a single spanning transmembrane (TM) protein whose function is still largely unknown but is believed to be involved in neurite outgrowth, neuronal protein trafficking, signal transduction, cell adhesion, and calcium metabolism. As shown in FIG. 2, the 15-16 amino acid N-terminal Aβ fragment (-N-Aβ fragment") is produced via an α-secretase-linked pathway (α- and |i-secretases) and is present at significant levels in cerebrospinal fluid (CSF).

[0005] The plaques are primarily composed of fibrillar β-amyloid, but the noted development of synaptic impairment is best correlated with the level of soluble Aft early in AD. followed by the accumulation of neurofibrillary tangles. In the absence of disease, the production of Aβ from synapses and the high rate of turnover of soluble Aβ raise the possiblity that the peptide can function as a neuromodulator under normal physiological conditions. Later, as Aβ accumulates, its action may then become disruptive, leading to compromised synaptic signaling.

[0006] Currently approved treatments for Alzheimer's disease (and related dementias) are limited to transient relief of early symptoms (e.g. short-term memory deficit) with no significant impact on the state or progression of the disease, particularly neurodegeneration. In addition, the current treatments are only effective in a portion of the population of individuals with Alzheimer ^' s disease and only for a limited period of time. To date, there is no effective cure for the disease, but identifying and characterizing the cellular mechanisms underlying AO will potentially elucidate a target pathway for potential treatment.

[0007] Lawrence et al. has recently shown that at low concentrations (picomolar (pM)-nanomolar (nM)), the N-terminal Aβ fragment is nearly twice as effective as full-length Aβ as a neuromodulator, stimulating receptor-linked increases in Ca ⁷ ' . enhancing long-term potentiation (LTP) and enhancing contextual fear conditioning (Lawrence et al.. J. Neurosctence. 34: 14210-14218. 2014). The activity of the N-Aβ fragment was localized to a core hexapeptide sequence within the N-Aβ fragment ("Aβ core sequence"). The presence of high-affinity nicotinic acetylcholine receptors (nAChRs) was found to sensitize neurons to Alt- induced toxic effects (Arora et al.. J. Biol. Chem, 288: 11175-11190. 2013). Models show that application of high concentrations (μΜ levels) of Aβ induces apoptosis in the absence of nAChRs.

[0008] As will be described herein, the present invention focuses on the potential activity of the core sequence in neurotoxicity and synaptotoxicity models. Without wishing to limit the invention to a particular theory or mechanism, it is proposed that nAChRs sensitize Aβ -induced neuronal death by intersecting with a common neurotoxicity pathway involving distinct Aβ target receptor(s). The N-terminal A)l fragment were found to inhibit this neurotoxicity and reverse inhibition of synaptic dynamics triggered by full-length Aβ . suggesting the possibility of neuroprotection.

SUMMARY OF THE INVENTION

[0009] The inventors have discovered a core six amino acid sequence in beta amyloid. Tyrosine- Glutamate-Valine-Hlstidine-Histidine-Glutamine (YEVHHQ. SEQ No. 1. single letter abbreviation), which may be referred to herein as "core ^* or "Αβ core ^" . Unexpectedly, this core demonstrates potent and highly effective neuroprotective activity against the toxic action of beta amyloid in killing synapses and nerve cells. The present invention transforms this peptide sequence into a stabilized derivative and/or a peptidomimetic (non-peptide small molecule), which may be used as a therapeutic for treatment of Alzheimer's disease and related dementias.

[0010] The subject disclosure is directed to active stabilized peptide derivatives (biologic) and/or peptidomimetics (synthetic) derived from the Aβt core sequence. Without wishing to limit the invention to any theory or mechanism, it is believed that the technical feature of the present invention advantageously overcomes the issues pertaining to the delivery of peptides into the brain, thereby allowing protection against synaptic dysfunction and neurodegeneration in the brain arising from β-amyloid toxicity, such as those associated with Alzheimer's disease and other related neurodegenerative disorders. The present invention is a unique approach that is qualitatively better and more effective, and that it benefits a larger portion of the population. More importantly, the present invention has the potential to significantly slow down or arrest the neurodegenerative process. None of the presently known prior references or work has the unique inventive technical feature of the present invention.

[0011] Any feature or combination of features described herein are included within the scope of the present invention provided that the features included in any such combination are not mutually inconsistent as will be apparent from the context, this specification, and the knowledge of one of ordinary skill in the art. Additional advantages and aspects of the present invention are apparent in the following detailed description and claims.

BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The features and advantages of the present invention will become apparent from a consideration of the following detailed description presented in connection with the accompanying drawings in which:

[0013] FIG. 1 A shows a diagrammatic representation of presynaptic regulation occurring via nerve activity and /or resident receptors.

[0014] FIG. 1B shows a model of how plaques and tangles affect the synapse (Wong et al.. Nature Neurosci. 5. 633-639. 2002).

[0015] FIG. 1C shows a model of progression of AD pathology (Ingelsson et al., Neurol. 62, 925-932, 2004).

[0016] FIG. 2 shows the functional domains in β-amyloid. ((-amyloid is cleaved from the amyloid precursor protein (APP) by the action of the B- and γ-secretases. and encompasses part of the APP transmembrane domain (TM). The N-terminal fragment (from the left, is produced from β-amyloid by the action of a- secretase.

[0017] FIG. 3 shows the β-amyloid core fragment and [(-amyloid in a competitive mechanism (direct or via differential receptor-based signaling).

[0018] FIG. 4 shows that the N-Aβ fragment protects against Aβ-induced oxidative stress in the presence of high-affinity AB receptor (nicotinic receptors: nAChRs) in a differentiated neuronal cell line (NG108-15). Concentrations for combination daily treatments were 10nM. lOOnM or 500nM of either Ν-Αβ fragment or N-ABcore in combination with 100nM Αβ1-42. Timing experiments were performed with Ν-Αβ fragment (100nM) introduced one day prior, co-treated, or 1 day post-treated with Αβ1-42 (100nM) (n=4 experiments).

[0019] FIGs. 5A-5B show that the Aβ core sequence (N-Aβtcore) protects against Aβ)-induced cellular toxicity in the presence of high-affinity Aβ receptor. FIG. 5A shows oxidative stress (ROS. reactive oxygen species) and FIG. 5B shows DNA fragmentation in apoptosis. Treatment is with "lOOnM N-ABcore after 0-, 1-, 2-day treatment of 100nM Αβ1-42 in the presence a4P2-nAChR (n=5 experiments).

[0020] FIGs. 6A-6B show that the Aflcore sequence and N-AβS fragment protect against Aft-induced cellular toxicity in the absence of high-affinity Aβi receptor (nAChR). FIG. 6A shows oxidative stress (ROS) and FIG. 6B shows DNA fragmentation in apoptosis Treatment of lOOnm or ΐμΜ Ν-Αβ core or Ν-Αβ fragment is alone and in combination with 1uM Αβ1-42 (n=5 experiments). Note that in the absence of nAChRs. μΜ concentrations of Αβ1-42 are required to increase ROS. Co-treatments with Αβ1-42 are represented by closed (grey) bars.

[0021] FIGs. 7A-7B show that the N-Aβ» fragment and N-Aβi core sequence protect against Aβ)-induced cellular toxicity (oxidative stress (ROS)) in mouse primary hippocampal neurons. FIG. 7A shows 5-day and FIG. 7B shows 10-day daily treatments with 1μΜ Αβ1-42, 1μΜ Ν-Αβ fragment, or 1μΜ N-ABcore and combination treatments of 1μΜ Αβ1-42 and 1μΜ Ν-Αβ fragment or 1μΜ N-ABcore (n=4 experiments).

[0022] FIG. 8 shows that the N-AB fragment protects against cellular toxicity (oxidative stress: ROS) induced by endogenously produced Aβ) (from transfected APP). [0023] FIGs. 9A-9B show the requirement for the histkJine residues in the N-Aβ core sequence and the stabilization of the N-Aβ core sequence by N-acetylation and C-amidation in the protection against Alt- induced cellular toxicity. FIG. 9A shows co-treatment of 100nM Αβ1-42 with 100nM [H13A.H13A] N- Aβ core or 100nM [Q15H] N-Aβ core (n=4 experiments). FIG. 9B shows daily co-treatment with 100nM Αβ1-42 and 100nM capped-Aβ core. D-[Apcore] or Capped-D-[A0core] reduces ROS. Mock-transfected cells are represented by open bars: α4β2- nAChR-transfected cells are represented by closed (grey) bars.

[0024] FIGs. 10A-10B show that the N-terminal Aβ fragment is non-toxic and protects against Aβ -induced cell death. FIG. 10A shows normalized cell counts in a model neuronal cell lines (NG108-15 cells) with, or without. o4P2-nAChRs treated daily with 100nM Αβ1-42, Ν-Αβ fragment or N-Aβ core alone, or combination treatments with Αβ1-42 and Ν-Αβ fragment or N-Aβ core over 7 days (n=4 experiments). FIG. 10B shows cell counts in primary hippocampal cell cultures (mouse primary hippocampal neurons) treated daily with 1μΜ Αβ1-42. Ν-Αβ fragment or N-Aβ core alone, or combination daily treatments with Αβ1-42 and Ν-Αβ fragment or N-Aβ core for 10 days (n=4 experiments).

[0025] FIG. 11 shows that the N-Aβ) fragment protects against Aβ-linked deficits in synaptic plasticity (from Lawrence et al.. J Neurosci. 34. 14210-14218. 2014).

[0026] FIG. 12 shows that the N-Aβ fragment protects against Aβ -linked deficits in contextual fear memory in 5XFAD (familial Alzheimer ^' s disease) APP mice.

[0027] FIGs. 13A-13C show structure-activity analysis of the N-Aβ core sequence, peptide stabilization and comparison to kinetics of Aβ . In FIG. 13A. the top graph shows average peak Ca" responses in varicosities of cells expressing a7-nAChRs to 100nM Αβ1-42 (n=44). N-ABcore (n=70). Ν-Αβ fragment (n=178). [Y10A] N-Aβ core (n=14). [H13A.M14A] N-Aβ core (n=19). and [Q15H] NAβ core (n=33). The bottom gaph shows average peak Ca'* responses in varicosities of cells expressing a4P2-nAChRs to 100nM Αβ1-42 (n=36), N-Aβ core (n=32), Ν-Αβ fragment (n=35), [Y10A] N-Aβ core <n=21 ). [H13A.H14A] N-ABcore (n=19). and [Q15H] N-Aβ core (n=29). Averaged data are means t SEM and dashed lines indicate the baseline (background) and average maximal responses of Ν-Αβοοre. *p<0.05 and ***p<0.0001. FIG. 13B. top. shows average peak Ca ² * responses in varicosities of cells expressing α4β2- nAChRs to 100nM N-Aβ core (n=51 ) and "capped'-Aβ core (n=23). Protection of N-Aβ core via N-terminal acetyfation and C-terminal amidation (capped) retains activity compared to N-Aβ core. FIG. 13B. bottom, shows peak responses to 100nM D-[Aβ core] (n=57) and Capped-D-[Aβ core] (n=65). Enantiomer conversion and stabilization of N-Aβ core retains activity. Averaged data are mean ± S.E.M. and dashed lines indicate the average maximal responses of N-Aβ core. FIG. 13C shows average Ca ^z + responses (F/Ffi) in varicosities of NG108-15 cells expressing α4P2-nAChRs to 100nM N-Aβ core (n=33), 100nM N- Αβ Fragment (n=17) and 100nM Aβ ₁ _ ₁₇ (n=40) over 13 minutes of treatment. Averaged data are means ± SEM and n represents the total number of varicosities examined

[0028] FIGs. 14A-14B Ν-Αβ core sequence rescue of DNA fragmentation in apoptosis (TUNEL staining) with or without nAChRs in the differentiated neuronal cell line (NG108-15). FIG. 14A shows co-treatment with 100nM N-Aβ core after 0-. 1-. 2-, 3-day treatment of 100nM Αβ1-42 in the presence of α4β2-nAChRs (n=3 experiments). Mock-transfected cells are represented by open bars: ο4β2- nAChR-transfected cells are represented by closed (grey) bars. FIG. 14B shows daily treatment of 100nM or 1 μΜ N-Aβ core or N- Αβ fragment alone and in combination with 1 μΜ Αβ1-42 (n=3 experiments). Note that in the absence of nAChRs. μΜ concentrations of Αβ1-42 are required to induce cell death. Co-treatments with Αβ are represented by closed (grey) bars.

DETAILED DESCRIPTION OF THE INVENTION

[0029] As the neuroprotective potential of the N-terminal Αβ fragment to inhibit and/or reverse cellular neurotoxicity and synaptic dysfunction triggered by full-length Aβ through target receptors was shown to reside in the N-Aβ(core. this present invention focuses on derivation and/or stabilization of the core sequence and analogues thereof.

[0030] As used herein, the "natural amino acids" refers to the twenty amino acids that are found in nature, i.e. occur naturally. The natural amino acids are as follows: alanine, arginine, glycine, asparagine, aspartic acid, cysteine, glutamine. glutamic acid, serine, threonine, histidine. lysine, methionine, proline, valine, isoleucine, leucine, tyrosine, tryptophan, and phenylalanine. This application adheres to the lUPAC rules of standard abbreviations for amino acids.

[0031] As used herein, the "unnatural amino acids ^" refers to amino acids that are not naturally encoded or found in the genetic code of any organisms. Typically, the unnatural amino acids are different from the twenty naturally occurring amino acids in their side chain functionality.

[0032] A "peptide" is defined as an amino acid sequence from three amino acids to about 700 amino acids in length. As used herein, a "peptide analogue" includes allelic variants: fragments: derivatives; substitution, deletion, insertion variants, fusion polypeptides and orthologs. Each amino acid of each such related peptide analogue may be either natural or unnatural of the "D" or "L" configuration which corresponds to the stereochemical designation "S" and "R." respectively, as defined in the RS system of Cahn et al., (Pure Aβ plied Chemistry, 45:11-30 (1974), and references cited therein). As known to one of ordinary skill in the art, only L-amino acids are manufactured in cells and incorporated into proteins. As used herein, the letter "D" preceding any three-letter abbreviation for an amino acid, e.g. as in "D-His", denotes the D-form of the amino acid, and a lack thereof refers to the L-form.

[0033] As defined herein, the term "agonist" refers to compound that enhances a response. The agonist binds to the same site as the endogenous compound and produces the same type of signal, usually of equal or greater magnitude than the endogenous agent. As defined herein, the term "antagonist" refers to compound that binds to the same site as the endogenous compound and diminishes or blocks the signal generated by the endogenous agent.

[0034] As used herein, for each amino acid, the amino acid residue of the peptide analogue may be preserved, which is defined as keeping said amino acid in the sequence. However, preserved does not imply unmodified. The amino acid may be preserved, but can also be modified. For example, a Histidine residue may be modified to a D-His. N-alkylated His (e.g. N-methylated His), or a β-substituted His.

[0035] In contrast, a conservative substitution, as known to one of ordinary skill in the art. refers to a complete replacement of an amino acid residue with a different residue having similar biochemical characteristics, such as size, charge, polarity, etc. For instance, the aromatic Tyrosine may be conservatively substituted with aromatic phenylalanine, or basic Arginine may be conservatively substituted with basic Lysine. TABLE 1 A and 1 B show non-limiting examples of conservative amino acid substitutions.

[0036] TABLE 1A

[0037] TABLE 1B

[0038] As defined herein, the term "W-methylation" refers to a form of alkylation wherein a methyl group, CH ₃ . replaces the hydrogen atom of the NH moiety in the backbone amide NHs of the peptide analogue.

[0039] β-Subatitution

[0040] For each amino acid, a (1-amino acid or β-peptide refers to an amino acid in which the amino group of -NH _Z is attached to the secondary carbon rather than the «/ carbon. For example, a methylene group (CH ₂ ) is inserted into the side chain at the beta position of that side chain. The flexibility to generate a vast range of stereo- and regioisomers. together with the possibility of disubstitutkxi. significantly expands the structural diversity of |!-amino acids. For instance, the incorporation of |i-amino acids has been successful in creating peptidomimetics that not only have potent biological activity, but are also resistant to proteolysis.

[0041] N-Termlnal Modification

[0042] As used herein, the N-terminal modification refers to the modification of the NH ₂ at the N-terminus, resulting in an R1 being linked to the NH moiety. N-terminal modifications are known to one of ordinary skill in the art. In some embodiments. N-terminal modification may comprise acylation. For example, an acylation modification comprising acetylation of the N-terminal results in R1 being an acetyl. -COCH,. such that the N-terminus is CH ₃ CO-HN-. In other embodiments. R1 may comprise a -CO-A, wherein A is an optionally substituted alkyl group. For instance. R1 may comprise

where "n" can range from 0 to 10. When n=0. the R1 of the N-terminal is an acetyl. Without wishing to limit the invention to a particular theory or mechanism. N-terminal modifications may play a role in stability, protein folding, cellular attachment, and function modulation of the peptide analogue.

[0043] C-Terminal Modification

[0044] As used herein, C-terminal modification refers to the modification of the carboxyl moiety at the C- terminus. C-terminal modifications are known to one of ordinary skill in the art. As used herein. R2 refers to the standard -COOH of the C-terminal. Examples of C-terminal modifications include, but are not limited to. amidation. esterification. or reduction. In some embodiments, the C-terminal may be modified to be -CONHR ₃ . -COO-A, or -CH ₂ OH. where R ₃ may be an H. NH ₂ . OH or an optionally substituted alkyl, and where A is selected from an optionally substituted alkyl group. For example, the C-terminal can be an amidated (e.g. -CONH ₂ ). Without wishing to limit the invention to a particular theory or mechanism, C- terminal modifications, such as amidation, can enhance the biological activity of the peptide analogue, increase its stability, efficacy, and ability to enter cells, as well as increase its ability to resist enzymatic degradation.

[0045] Glycosylation

[0046] As used herein, the term "glycosylated ^" is defined as a saccharide (or sugar) covalently attached, i.e. linked, to an amino acid, resulting in a glycan. Specifically, the saccharide is linked to the side-chain of the amino acid. In some embodiments, the peptide analogue may be modified via glycosylation or with a glycan. at the N-terminal or C-terminal. Glycosylation processes and glycans are well known to one of ordinary skill in the art. In one aspect, the glycosylated amino acid may comprise a saccharide O-linked. rV-linked, C-linked. or S-linked to a natural amino acid.

[0047] In some embodiments, the saccharide is attached to the hydroxyl group of the side-chain of the amino acid, such as Ser, Thr. or Tyr. In another aspect, the glycosylated amino acid may comprise a saccharide N-l inked to a natural amino. For example, the saccharide is attached to the amine group of the side-chain of the amino acid, such as Asn or Lys. In other embodiments, glycosylation of the C-terminal may take place via an esterification reaction. Examples of saccharides include, but are not limited to, glucose, fructose, and lactose. In another aspect, the glycan may be selected from mono-, di-. tri- and poiy-saccharides. In some embodiments, the glycosylated peptide analogue has an increased ability to cross a blood brain barrier (BBB) as compared to a peptide lacking the glycosylation.

|(HMK| isosteres

|IMI49| As known to one of ordinary skill in the art, replacing of the peptide bond, i.e. the CONH, results in a peptide isostere. Without wishing to limit the invention to a particular theory or mechanism, the application of peptide isosteres with the objective to increase the metabolic stability of oligopeptides and to reduce their conformational flexibility can provide for peptide based bioactive molecules. Peptide isosteres are not composed of alpha amino acids but have structures and properties resembling those of "common" peptides. For example, dipeptide isosteres are designed to replace two amino acids and are functionalized with an amine and a carboxylate functionality. Non-limiting example of groups that can replace the peptide bond include, but are not limited to ester, N-alkylation. alkene, alyneamkJe to double bond, thioamide. amideoxy, azapeptide. and dehydroaminoacid.

[0050] Cyclization

100511 The peptide analogues of the present invention may be cyclized thorough bridging of the residues via ring closing reactions. In particular, the side chain of a residue is linked to the side chain of another residue via a linker. In some embodiments, the linker L, is a carba. lactam, disulfide, thioether. or succinic linker. As understood by one of ordinary skill in the art, the linker is not limited to the aforementioned examples, and may depend upon the specific cyclization chemistry used to produce the cyclic peptide. As a non-limiting example, residues can be linked via an amide bond formation reaction, which may form a - (CH ₂ )-CO-NH-(CH ₂ ) _n - bridge, where n=1 ,2,3.4. In addition, carbon-carbon bonds, lactone, thioether, ether, disulfide and other covalent bonds can be used as a part of the ring closing reactions. Without wishing to limit the invention to a particular theory or mechanism, the type of linker can affect the structural, chemical, and biological activity of the peptide ligarnd.

100521 Blood-Brain Barrier: The blood-brain barrier is made up of brain microvessel endothelial cells characterized by tight intercellular junctions, minimal pinocytic activity, and the absence of fenestra. These characteristics endow these cells with the ability to restrict passage of most small polar blood-borne molecules (e.g., neurotransmitter catecholamines, small peptides) and macromolecules (e.g., proteins) from the cerebrovascular circulation to the brain. The blood-brain barrier contains highly active enzyme systems as well, which further enhance the already very effective protective function. It is recognized that transport of molecules to the brain is not determined solely by molecular size but by the permeabilities governed by specific chemical characteristics of the permeating substance. Thus, besides molecular size and lipophilicity. the affinity of the substances to various blood proteins, specific enzymes in the blood, or the blood-brain barrier may influence the amount of the drug and/or peptide analogue reaching the brain.

100531 As used herein, the terms "treat", "treating-, or "treatment" refer to both therapeutic treatment and prophylactic or preventative measures, with the objective of preventing, reducing, slowing down (lessen), inhibiting, or eliminating an undesired physiological change, symptom, or disorder, such as the development or spread of β-amyloid toxicity, for example, β-amyloid deposits and Alzheimer's disease. For purposes of this invention, beneficial or desired clinical results include, but are not limited to. alleviation of symptoms, diminishment of extent of disease, stabilized (i.e.. not worsening) state of disease, delay or slowing of disease progression, amelioration or paliation of the disease state, and remission (whether partial or total), whether detectable or undetectable. For example, treatment with the peptide analogue of the invention may include reduction of undesirable β-amyloid toxicity, such as oxidative stress, synaptic dysfunction, and apoptosis or cell death. "Treatment" can also mean prolonging survival as compared to expected survival if not receiving treatment. Those In need of treatment include those already with the condition or disorder as well as those prone to have the condition or disorder or those in which the condition or disorder is to be prevented or onset delayed. Optionally, the subject or patient may be identified (e.g., diagnosed) as one suffering from the disease or condition (e.g.. Alzheimer's disease) prior to administration of the peptide analogue of the invention.

[0054] A "therapeutically effective amount ^" refers to an amount that is sufficient to achieve the desired therapeutic result or to have an effect on undesired symptoms, but is generally insufficient to cause adverse side effects. The specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including the disease being treated and the severity of the disease; the specific composition employed; the age, body weight, general health, sex and diet of the patient: the time of administration; the route of administration: the rate of excretion of the specific compound employed; the duration of the treatment: drugs used in combination or coincidental with the specific compound employed and like factors well known in the medical arts. For example, it is well within the skill of the art to start doses of a compound at levels lower than those required to achieve the desired therapeutic effect and to gradually increase the dosage until the desired effect is achieved. If desired, the effective daily dose can be divided into multiple doses for purposes of administration. Consequently, single dose compositions can contain such amounts or submultiples thereof to make up the daily dose. The dosage can be adjusted by the individual physician in the event of any contraindications. Dosage can vary, and can be administered in one or more dose administrations daily, for one or several days. Guidance can be found in the literature for appropriate dosages for given classes of pharmaceutical products.

|U055| A "subject ^' ' is an individual and includes, but is not limited to. a mammal (e.g., a human, horse, pig, rabbit, dog, sheep, goat, non-human primate, cow. cat. guinea pig. or rodent), a fish, a bird, a reptile or an amphibian. The term does not denote a particular age or sex. Thus, adult and newborn subjects, as well as fetuses, whether male or female, are intended to be included. A "patient ^" is a subject afflicted with a disease or disorder. The term "patient" includes human and veterinary subjects.

[0056] The terms "administering ^" and "administration ^" refer to methods of providing a pharmaceutical composition to a subject. Such methods are well known to those skilled in the art and include, but are not limited to. administering the compositions orally, parenterally (e.g.. intravenously and subcutaneously). by intramuscular injection, by intraperitoneal injection, intrathecal^, transdermally, extracorporeal^, topically or the like.

|IK)57| As described above, the compositions can be administered to a subject in a pharmaceutically acceptable carrier. By "pharmaceutically acceptable" is meant a material that is not biologically or otherwise undesirable, i.e., the material may be administered to a subject without causing any undesirable biological effects or interacting in a deleterious manner with any of the other components of the pharmaceutical composition in which it is contained. The carrier would naturally be selected to minimize any degradation of the active ingredient and to minimize any adverse side effects in the subject, as would be well known to one of skill in the art.

|U05X| Pharmaceutical carriers are known to those skilled in the art. These most typically would be standard carriers for administration of drugs to humans, including solutions such as sterile water, saline, and buffered solutions at physiological pH. Typically, an appropriate amount of a pharmaceutically- acceptable salt is used in the formulation to render the formulation isotonic. Examples of the pharmaceutically-acceptable carrier include, but are not limited to, saline. Ringer's solution and dextrose solution. The pH of the solution may be about 5 to about 8. such as from about 7 to about 7.5. Further carriers include sustained release preparations such as semi-permeable matrices of solid hydrophobic polymers containing the disclosed compounds, which matrices are in the form of shaped articles, e.g., films, liposomes, microparticles. or microcapsules. It will be apparent to those persons skilled in the art that certain carriers can be more desirable depending upon, for instance, the route of administration and concentration of composition being administered. Other compounds can be administered according to standard procedures used by those skilled in the art.

[0059] Pharmaceutical formulations can include additional carriers, as well as thickeners, diluents, buffers, preservatives, surface active agents and the like in addition to the compounds disclosed herein. Pharmaceutical formulations can also include one or more additional active ingredients such as antimicrobial agents, anti- inflammatory agents, anesthetics, and the like.

|U06U| The pharmaceutical formulation can be administered in a number of ways depending on whether local or systemic treatment is desired, and on the area to be treated. Administration may be topically (including ophthalmically. vaginally, rectally. intranasaly). orally, by inhalation, or parenterally. for example by intravenous drip, subcutaneous, intraperitoneal or intramuscular injection. The disclosed compounds can be administered intravenously, intraperitoneally, intramuscularly, subcutaneously. intracavity, or transdermally.

|U06l | For example, preparations for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil. fish oils, and injectable organic-esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media. Parenteral vehicles include sodium chloride solution. Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's, or fixed oils. Intravenous vehicles include fluid and nutrient replenishes, electrolyte replenishes (such as those based on Ringer's dextrose), and the like. Preservatives and other additives may also be present such as. for example, antimicrobials, anti-oxidants. chelating agents, and inert gases and the like.

[0062] Pharmaceutical formulations for topical administration include but are not limited to, ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Conventional pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable. Pharmaceutical formulations for oral administration include, but are not limited to. powders or granules, suspensions or solutions in water or non-aqueous media, capsules, sachets, or tablets. Thickeners, flavorings, diluents, emulsifiers, dispersing aids or binders may be desirable.

|0O631 Nerve cells, of which there are nearly 100 billion in the human brain, communicate by special contacts called 'synapses ^' (FIG. 1A). Complex communication in the brain occurs via approximately 100 trillion synapses. Each synapse is highly dynamic and can be regulated positively or negatively, short- and long-term potentiation, and long-term depression. Prior to AD. Αβ is released from nerve endings at synapses in response to nerve activity. Αβ in pM-nM concentrations has agonist-like activity at presynaptic nicotinic receptors (nAChRs) and can increase Ca ² + in isolated synaptic terminals via nAChRs. In pM concentrations, Αβ augments long-term potentiation. Therefore, Αβ is active as a neuromodulator.

100641 In FIG. 1B. the plaques, which are composed of a sticky peptide called beta amyloid (Αβ), accumulate in the brain in AD, primarily near synapses. Abnormal tau, manifested as neurofibrillary tangles, is linked to neurotoxicity leading to neurodegeneration. In AD. the most notable early change is the dramatic loss of synapses. Referring to FIG. 1C, most beta amyloid is soluble and diffusible, therefore "floating" around the brain. Its levels rise sharply 5-15 years before the diagnosis of AD. After this rise, synapses and nerve cells start to die off.

[0065] As discussed. FIG. 2 shows that [l-amyloid is cleaved from the amyloid precursor protein (APP). N- terminal Aβ fragments (1-14: 1-15: 1-16) are generated by the combined action of "</ and β-secretases. and resident carboxypeptidase. The N-terminal Aβt fragment is present in the brains and CSF of healthy adults as well as in Alzheimer ^' s patients. Unlike full-length oligomeric |5-amyloid. the N-terminal Aβ fragment is monomelic and nontoxic. In Ca ² ' imaging studies, the N-terminal Aβt fragment has proven to be highly potent and more effective than full-length β-amyloid in its agonist-like action on nicotinic receptors. N-terminal Aβ fragments increase when Aβ production is blocked under conditions of reduced y-secretase activity.

[0066] Referring to FIG. 3, although high levels of β-amyloid interferes with synaptic dynamics and kills neurons, the addition of the non-toxic N-terminal Aβ fragments can prevent synaptic inhibition and cell death. Without wishing to limit the present invention to particular theory or mechanism, these N-terminal Aβ fragments are believed to participate in a direct or indirect competitive mechanism against the β- amyloid and/or Aβ-linked intracellular signaling pathways, hence, these N-fragments are neuroprotective. The present invention features peptide derivatives and peptidomimetics based on the neuroprotective core sequence fragment for use in treating Alzheimer's disease.

[0067] According to one embodiment, the present invention features a modified [l-amyloid peptide analogue comprising a sequence at least 50% identical to YEVHHQ (SEQ ID NO. 1 ). In preferred embodiments, the HH is preserved. In some embodiments, any one of the residues may be conservatively substituted. In one embodiment, one. two. or three residues may be conservatively substituted. In another embodiment, the modified β-amyloid peptide analogue comprises a sequence that is at least 60% identical to SEQ ID NO. 1. For example, one or two residues may be conservatively substituted. In yet another embodiment, the modified β-amyloid peptide analogue comprises a sequence that is at least 80% identical to SEQ ID NO. 1. For example, at most one residue may be conservatively substituted. For example, the Q may be conservatively substituted with H. In further embodiments, the Y residue is preserved. In most instances, it may be desirable that any of the peptide analogues of the present invention be non-toxic and neuroprotective. Non-limiting examples of analogues based on SEQ ID NO. 1 are provided in Table 2.

|U06K| TABLE 2. Sequences of modified ((-amyloid peptide analogues based on SEQ ID NO. 1. *D-amino Acid, N-methylated, Beta-substituted amino acid. refers to the residue being a D-amino Acid and/or N-methylated and/or a Beta-substituted amino acid. R1 can be an acetyl, glycosylate, or -CO-(CH ₂ ) _n CH ₃ from N-terminal modifications. R2 can be amidation. reduction or glycosylation from C-terminal modifications. In some embodiments, n can range from 1 to 10. It is understood that other permutations are possible and within the scope of the invention.

|0069| According to yet another embodiment, the present invention features a modified β-amyloid peptide analogue comprising a sequence at least 50% identical to DAEFRHDSGYEVHHQ (SEQ ID NO. 2). with the caveat that HH is preserved. For example, at least 8 residues, including HM. of SEQ ID NO. 2 are preserved. According to another embodiment, the modified β-amyloid peptide analogue may comprise a sequence that is at least 60% identical to SEQ ID NO. 2. For example, at least 9 residues, including HH. of SEQ ID NO. 2 are preserved. According to yet another embodiment, the modified β-amyloid peptide analogue may comprise a sequence that is at least 70% identical to SEQ ID NO. 2. For example, at least 11 residues, including HH. of SEQ ID NO. 2 are preserved. According to a further embodiment, the modified |i-amyk>id peptide analogue may comprise a sequence that is at least 80% identical to SEQ ID NO. 2. For example, at least 12 residues, including HH, of SEQ ID NO. 2 are preserved. In an exemplary embodiment, the Y residue is preserved. In another exemplary embodiment. Q may be conservatively substituted with H. In most instances, it may be desirable that any of the peptide analogues of the present invention is non-toxic and neuroprotective.

[0070] In some embodiments, at most 7 residues of the 1 ^st - 12 ^th and 15 ^lh residues in SEQ ID NO. 2 may be conservatively substituted. In other embodiments, at most 6 residues of the and 15'" residues in SEQ ID NO. 2 may be conservatively substituted. In stil other embodiments, at most 5 residues of the 1 ^st - 12"' and 15 ^'' residues in SEQ ID NO. 2 may be conservatively substituted. In one embodiment, at most 4 residues of the r'-12 ^w and 15* residues in SEQ ID NO. 2 may be conservatively substituted. In another embodiment, at most 3 residues of the 1^-12" and 15 ^th residues in SEQ ID NO. 2 may be conservatively substituted. In yet another embodiment, at most 2 residues of the 1 ^M -12' ^' ' and 15* residues in SEQ ID NO. 2 may be conservatively substituted. In a further embodiment, only 1 residue of the Γ'-12" ^° and 15"' residues in SEQ ID NO. 2 may be conservatively substituted. Non-limiting examples of said analogues based on SEQ ID NO. 2 are provided in Table 3.

|U07I| TABLE 3. Sequences of modified |i-amyloid peptide analogues based on SEQ ID NO. 2. *D-amino Acid. N-methylated. Beta-substituted amino acid. refers to the residue being a D-amino Acid and/or

N-methylated and/or a Beta-substituted amino acid. R1 can be an acetyl, glycosylate, or -CO-(CH ₂ ) _n CH ₃ from N-terminal modifications. R2 can be amidation. reduction or glycosylate from C-terminal modifications. In some embodiments, n can range from 1 to 10.lt is understood that other permutations are possible and within the scope of the invention.

|00721 According to another embodiment, the present invention features a modified |!-amylokl peptide analogue being at least 50% identical to at least a YEVHHQ fragment of DAEFRHDSGYEVHHQ (SEQ ID NO. 2), with the caveat that HH is preserved. In one embodiments, the peptide analogue may comprise a sequence according to the following:

1007.31 In another embodiment, the peptide analogue may be at least 60% identical to YEVHHQ of SEQ ID NO. 2. In yet another embodiment the peptide analogue is at least 80% identical to YEVHHQ of SEQ ID NO. 2. In some embodiments, any of the X ₁ -X ₉ residues may be optionally present in the peptide analogue. In other embodiments, one or more residues from X ₁ -X ₉ may be absent (i.e., no amino acid may be in those positions). In one embodiment, the first amino acid of the peptide analogue may be at position In other embodiments, the residue may be optionally

present, i.e. the X ₁₅ residue may be absent. For example, the last amino acid may be the terminal His.

[0074] In some embodiments, the peptide analogue is at least 5 amino acids in length. In other embodiments, the peptide analogue is at least 6 amino acids in length. In yet other embodiments, the peptide analogue is at least 7 amino acids in length. In stil other embodiments, the peptide analogue is at least 8 amino acids in length. In further embodiments the peptide analogue is at least 9 amino acids in length. In some embodiments, the peptide analogue is at least 10 amino acids in length. In other embodiments, the peptide analogue is at least 11 amino acids in length. In yet other embodiments, the peptide analogue is at least 12 amino acids in length. In still other embodiments, the peptide analogue is at least 13 amino acids in length. In further embodiments, the peptide analogue is at least 14 amino acids in length. In still further embodiments, the peptide analogue is at least 15 amino acids in length.

[0075] In one embodiment. X ₁ may be Asp, or a conservative substitution thereof (e.g. Asn. Gin, Glu. Ser). Conservative substitutions for each natural amino acid are listed in TABLE 1A and 1B. In another embodiment. X ₂ may be Ala. or a conservative substitution thereof. In yet another embodiment, X. _t may be Glu. or a conservative substitution thereof. In a further embodiment. X ₄ may be Phe. or a conservative substitution thereof. In yet a further embodiment. X ₅ may be Arg. or a conservative substitution thereof. In one embodiment, Xg may be His. or a conservative substitution thereof. In another embodiment. X, may be Asp, or a conservative substitution thereof. In yet another embodiment, Xg may be Ser. or a conservative substitution thereof. In still another embodiment. X _g may be Gly. or a conservative substitution thereof. In a further embodiment, the X _K may be Tyr that is preserved. In an alternative embodiment. X ₁₀ may be Tyr, or a conservative substitution thereof. In yet a further embodiment X _{1 1} may be Glu. or a conservative substitution thereof. In still a further embodiment. X ₁₂ may be Val, or a conservative substitution thereof. In one embodiment. X ₁₅ may be Gin. His or a conservative substitution thereof. In yet a further embodiment, X ₁ - X ₁₂ and X ₁₅ may each be independently selected from natural and unnatural amino adds.

[0076] According to yet another embodiment, the modified μ-amyloid peptide analogue is derived from DAEFRHDSGYEVHHQ (SEQ ID NO. 2), with a caveat that HH is preserved. In one embodiment, the peptide analogue may comprise a sequence according to the following:

Consistent with previous embodiments, the residues may optionally be present in the peptide analogue. In other embodiments, t esidues may each be independently selected from

natural and unnatural amino acids.

|U077| Non-limiting examples of said peptide analogues according to SEQ ID NO. 3 are provided in Table 4. It is understood that other permutations are possible and within the scope of the invention.

[U07K| TABLE 4. Sequences of modified |S-amyloid peptide analogues according to SEQ ID NO. 3. ^* D- amino Acid, N-methylated, Beta-substituted amino acid. refers to the residue being a D-amino Acid and/or N-methylated and/or a Beta-substituted amino acid. R1 can be an acetyl, glycosylation, or -CO- (CH ₂ ) _h CH ₃ from N-terminal modifications. R2 can be amidation, reduction or glycosylation from C-terminal modifications. In some embodiments, n can range from 1 to 10. In some embodiments, n can range from 1 to 10.

[0079] In preferred embodiments, il may be desirable that the peptide analogues of the present invention be non-toxic and neuroprotective. Without wishing to be bound to a particular theory or mechanism, the peptide analogues of the present invention are capable of competitive binding against β-amyloid for target sites or activation of pathways, such as alternative pathways (e.g. anti-apoptotic pathways and/or anti- oxidative pathways), thereby blocking or reversing the process by which the β-amyloid induces neurotoxicity.

|(HNtO| In some embodiments, any of the modified β-amyloid peptide analogues described herein may comprise an N-terminal modification such as. for example, acetylation or glycosytation. In other embodiments, any of the modified β-amytoid peptide analogues may comprise a C-terminal modification such as, for example, amidation. esterification or glycosylation. In further embodiments, any of the modified β-amyloid peptide analogues may be cyclized.

[IMMCI I In one embodiment, at least one of the residues in SEQ ID NO. 1. SEQ ID NO. 2. or SEQ ID NO. 3 may be n-methyiated. In another embodiment, at least one of the residues in SEQ ID NO. 1. SEQ ID NO. 2. or SEQ ID NO. 3 may be a D-amino acid. In a further embodiment, at least one of the residues in SEQ ID NO. 1. SEQ ID NO. 2. or SEQ ID NO. 3 may be a ((-substituted amino acid. In yet another embodiment, at least one of the residues in SEQ ID NO. 1. SEQ ID NO. 2. or SEQ ID NO. 3 can have multiple modifications. For example, the residue may be a D-amino acid that has been N-methylated. In other embodiments, at least one of the residues in SEQ ID NO. 1. SEQ ID NO. 2. or SEQ ID NO. 3 may be a peptide isostere.

|U082| According to another embodiment, the present invention features a method of treating Alzheimer's disease in a subject in need of such treatment. The method may comprise administering to the subject a therapeutically effective amount of a composition comprising any of the modified JV-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier. Without wishing to limit the invention to a particular theory or mechanism, it is believed that the peptide analogues can be administered in a manner to effectively reduce or terminate synaptic inhibition and neuron death, thereby reducing the neurodegenerative effects of Alzheimer's disease.

|lMMO I According to a further embodiment, the present invention features a method of preventing Alzheimer's disease in a subject. The method may comprise administering to the subject a therapeutically effective amount of a composition comprising any of the modified |!-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier. Without wishing to limit the invention to a particular theory or mechanism, it is believed that the peptide analogues can be administered in a manner to effectively prevent synaptic inhibition and neuron death, thereby preventing Alzheimer's disease in the subject.

|00K4| According to some embodiments, the present invention features a method of treating or preventing a symptom associated with Alzheimer's Disease (AD) in a subject. In exemplary embodiments, the symptom may be associated with β-amyloid deposits, such as β-amyloid plaques. The method may comprise administering to the subject a therapeutically effective amount of a composition comprising any of the modified β-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier. Without wishing to be bound to a particular theory or mechanism, the peptide analogue may be administered in a manner to effectively terminate synaptic inhibition and neuron death. |INNt5| According to other embodiments, the present invention features a method of treating or preventing a symptom associated with β-amyloid-induced toxicity in a subject. In exemplary embodiments, the β- amyloid-induced toxicity may be associated with oxidative stress and apoptosis. The method may comprise administering to the subject a therapeutically effective amount of a composition comprising any of the modified β-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier.

[0086] According to further embodiments, the present invention features a method of treating, reducing or inhibiting a disease or condition in a subject by administering to the subject a therapeutically effective amount of a composition comprising any of the modified β-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier. In exemplary embodiments, the disease or condition may be associated with synaptic inhibition or neuron death, such as. for example. Alzheimer ^' s disease.

[0087] In some embodiments, the present invention features a composition for use in treating or preventing Alzheimer's disease. The composition may comprise any of the modified |i-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier. In preferred embodiments, the composition is administered to a subject, where the composition comprises a therapeutically effective amount of the modified β-amyloid peptide.

|(HNtX| In other embodiments, the present invention features a composition for use in treating or preventing a symptom associated with Alzheimer's Disease. The symptom may be associated with β-amyloid deposits, such as β-amyloid plaques. The composition may comprise any of the modified |!-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier.

[0089] In yet other embodiments, the present invention features a composition for use in treating or preventing a symptom associated with β-amytoid-induced toxicity. For example, the β-amyloid-induced toxicity may be associated with oxidative stress and apoptosis. The composition may comprise any of the modified β-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier.

|U090| In further embodiments, the present invention features a composition for use in treating, reducing or inhibiting a disease or condition being associated with synaptic inhibition or neuron death. The composition may comprise any of the modified |i-amyloid peptide analogues described herein, in a pharmaceutically acceptable carrier. In one embodiment, the disease may be Alzheimer's disease.

[009] I In preferred embodiments, the composition comprising a therapeutically effective amount of the modified β-amyloid peptide is administered to a subject. Without wishing to limit the invention to a particular theory or mechanism, the compositions described herein may be effective for delivenng the modified β-amyloid peptide analogue through the blood brain barrier. The modified |S-amyloid peptide analogue may also be effective to reduce or terminate synaptic inhibition and neuron death. Further stil, the modified |i-amyioid peptide analogue can reduce a neurodegenerative effect of Alzheimer's disease.

[0092] According to some embodiments, the present invention features the use of a therapeutically effective amount of any of the modified β-amyloid peptide analogues described herein in preparation of a medicament for treatment or prevention of Alzheimer's disease in a subject. In one embodiment, the medicament may comprise the modified β-amyloid peptide is in a pharmaceutically-acceptable carrier. The medicament may be administered to the subject twice a day. daily, or every other day. Also, the medicament may be administered to the subject intranasally. intravenously, transdermally. or orally. In some embodiments, the medicament is effective for delivering the modified |)-amyloid peptide analogue through the blood brain barrier. Without wishing to limit the invention to a particular theory or mechanism, the modified β-amyloid peptide analogue is effective to reduce or terminate synaptic inhibition and neuron death, or to reduce a neurodegenerative effect of Alzheimer ^' s disease.

[0093] According to other embodiments, the present invention features the use of a therapeutically effective amount of any of the modified β-amyloid peptide analogues described herein in preparation of a medicament for treatment or prevention of a symptom associated with Alzheimer's Disease . In exemplary embodiments, the symptom may associated with β-amyloid deposits, such as β-amyloid plaques. Without wishing to be bound by a particular theory or mechanism, the modified β-amyloid peptide analogue may be effective to prevent, reduce or terminate synaptic inhibition and neuron death.

[0094] In still other embodiments, the present invention features the use of a therapeutically effective amount of any of the modified β-amyloid peptide analogues described herein in preparation of a medicament for for treatment or prevention of a symptom associated with β-amyloid-induced toxicity. Without wishing to be bound by a particular theory or mechanism, the modified β-amyloid peptide analogue may be effective to prevent, reduce or terminate the β-amyloid-induced toxicity associated with oxidative stress and apoptosis.

[0095] According to further embodiments, the present invention features the use of a therapeutically effective amount of any of the modified |i-amyloid peptide analogues described herein in preparation of a medicament for treating, reducing or inhibiting a disease or condition being associated with synaptic inhibition or neuron death. In an exemplary embodiment, the disease may be Alzheimer ^' s disease. Without wishing to be bound by a particular theory or mechanism, the modified β-amyloid peptide analogue may be effective to prevent, reduce or terminate synaptic inhibition and neuron death.

|00V6| For any of the methods or uses of the composition described above, in some embodiments, the therapeutically effective amount of the modified β-amyloid peptide analogue is from about 0.001 mg/kg to 500 mg/kg of body weight, or any range in between. In some embodiments, the therapeutically effective amount is within the range of about 0.001 mg/kg to about 0.01 mg/kg. or within the range of about 0.01 mg/kg to about 0.1 mg/kg. or within the range of about 0.1 mg/kg to about 0.5 mg/kg. or within the range of about 0.5 mg/kg to about 1 mg/kg. In other embodiments, the therapeutically effective amount is within the range of about 1 mg/kg to about 2.5 mg/kg. or within the range of about 2.5 mg/kg to about 10 mg/kg, or within the range of about 10 mg/kg to about 50 mg/kg. In still other embodiments, the therapeutically effective amount is within the range of about 50 mg/kg to about 100 mg/kg. or within the range of about 100 mg/kg to about 250 mg/kg. or within the range of about 250 mg/kg to about 500 mg/kg. In another embodiment, the therapeutically effective amount is an amount that is therapeutically effective in a mouse model. In further embodiments, the therapeutically effective amount of the modified (l-amyloid peptide analogue may be administered twice a day, daily, or every other day. In still further embodiment, the therapeutically effective amount of the modified β-amyloid peptide analogue is administered intranasally, intravenously, transdermally. or orally.

|U097| The terms "dose" or 'dosage" as used herein refers to a physically discrete unit of a formulation appropriate for a subject to be treated (e.g.. for a single dose). Each dose contains a predetermined quantity of an active agent, e.g. the modified |}-amyloid peptide analogue, selected to produce a desired therapeutic effect when administered according to a therapeutic regimen (it being understood that multiple doses may be required to achieve a desired or optimum effect). The dose may be, for example, a volume of liquid (e.g.. an acceptable carrier) containing a predetermined quantity of one or more therapeutic agents, a predetermined amount of one or more therapeutic agents in solid form, a sustained release formulation or drug delivery device containing a predetermined amount of one or more therapeutic agents, etc. It will be understood, however, that the total daily usage of a formulation of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific effective dose level for any particular subject or organism may depend upon a variety of factors including the severity of the disease; activity of the specific active peptides employed; specific composition employed; age, body weight, general health, sex and diet of the subject; time of administration, and rate of excretion of the specific active peptides employed: duration of the treatment; drugs and/or additional therapies used in combination or coincidental with specific compound(s) employed, and like factors well known in the medical arts.

|U098| In some embodiments, the modified β-amyloid peptide analogue is administered in a dosage of about 0.001 mg/kg to 500 mg/kg of body weight, or any range in between. For example, the dosage of the peptide analogue may be 0.001 mg/kg of body weight or 0.01 mg/kg of body weight or 0.1 mg/kg of body weight. For example, a human weighing 55 kg may be administered about 0.055 mg of the peptide analogue per dose. In other embodiments, the composition is administered twice a day, daily, or every other day.

|uu9V| In one aspect, the modified β-amyk>id peptide analogue composition can be administered in an intravenous dosage. This dosage can be administered to a subject once daily or in divided dosages throughout a day, as determined by methods known in the art. In some embodiments of treatment, the dosage to a subject will require long term application to provide for a steady, long term stimulation of CNS tissue, which may require days, weeks, or months of treatment. It is contemplated that the dosage of the modified ({-amyloid peptide analogue composition can be administered as infrequently as once every week or every two weeks, or at any interval in between, depending on a subject's clinical response to the medication. Moreover, if a subject does not respond to the initial dosage and administration of the modified (Vamyloid peptide analogue composition, a person of skill can administer the medication daily for several days until such response occurs. A person of skill can monitor a subject's clinical response to the administration of the modified β-amyloid peptide analogue composition, and administer additional dosages as needed. It is contemplated that the modified β-amyloid peptide analogue composition can be administered to a subject on a daily basis, on an alternating daily basis, on a weekly basis, on a monthly basis, or at any interval in between.

|0aiMi| In another aspect, the modified β-amyloid peptide analogue composition can be administered to a subject transdermally, by using an adherent patch, by using iontophoresis, or by using any other method known to a person of skill. The dosage of the modified β-amyloid peptide analogue composition, administered transdermally can be given daily or infrequently as once every 1 to 4 weeks. A person of skill, monitoring a subject's clinical response and improvement, can determine the frequency of administration of the medication by methods known in the art.

|INII(M| In certain embodiments, the modified β-amyloid peptide analogue composition can be administered to a subject by topical intranasal administration (intranasally) or administration by inhalant, in a dosage taken once daily or in divided doses. Further, the medication can be administered as infrequently as once every 1 to 4 weeks. A person of skill, monitoring a subject's clinical response to the administration of the medication, can adjust the frequency of administration according to methods known in the art. As used herein, "topical intranasal administration" means delivery of the compositions into the nose and nasal passages through one or both of the nares and can comprise delivery by a spraying mechanism (device) or droplet mechanism (device), or through aerosolization of the composition. Administration of the compositions by inhalant can be through the nose or mouth via delivery by a spraying or droplet mechanism. As used herein, "an inhaler" can be a spraying device or a droplet device for delivering the modified β-amyloid peptide analogue, in a pharmaceutically acceptable carrier, to the nasal passages and the upper and/or lower respiratory tracts of a subject. Delivery can also be directly to any area of the respiratory system (e.g.. lungs) via intratracheal intubation. The exact amount of the compositions required will vary from subject to subject, depending on the species, age, weight and general condition of the subject, the severity of the disorder being treated, the particular composition used, its mode of administration and the like. Thus, it is not possible to specify an exact amount for every composition. However, an appropriate amount can be determined by one of ordinary skill in the art using only routine experimentation.

|UOI02| In another aspect, the modified β-amyloid peptide analogue composition can be administered to a subject in a dosage taken once dairy or in divided doses. Furthermore, the medication can be administered as infrequently as once every 1 to 4 weeks. A person monitoring a subject's clinical response can adjust the frequency of administration of the medication based on methods known in the art.

|00IU3| Transient reversible modification of the blood-brain barrier can be accomplished by osmotic opening, which is based on increasing capillary permeability by osmotically-induced shrinkage of the endothelial cells which caused widening of the intercellular tight junctions. The osmotic load is generally a hyperosmotic water-soluble agent such as mannitol or arabinose. Briefly, under general anesthesia, a transfemoral catheter is introduced into the internal carotid or vertebral artery and 100-300 ml infusion of 25% mannitol is administered at 6-10 mg/sec for 30 seconds. An intravenous infusion of the pharmaceutical composition is begun approximately five to seven minutes before the mannitol infusion and is continued for 15 minutes. The transfemoral catheter is removed and the patient observed for 24-48 hours.

|0ailM| Alternatively, the modified β-amyloid peptide analogue may be linked to the osmotic agent (mannitol, arabinose. glucose or other sugar moiety), and a single infusion may be used. Conventional techniques may be used to link the modified β-amyioid peptide analogue and the osmotic agent. The linked agent itself will then cause the osmotically-induced shrinkage of the endothelial cells in order to widen the tight intercellular junctions. The linked agent may be designed such that the modified β-amyloid peptide analogue is cleaved from the linked agent after the BBB has been crossed.

|IH)I»5| EXPERIMENTAL

|001(M>| Current experiments relating to the invention have been preclinical using cell culture and animal models. Without wishing to limit the invention to a particular theory or mechanism, the core six-amino-acid beta amyloid peptide (N-ABcore) has consistently prevented or reversed beta amyloid-induced neurotoxicity, inhibition of synaptic dynamics and spatial memory deficits.

|UOI07| In Vitro Neuronal Models. The studies were performed on the NG 108-15 rodent hybrid neuroblastoma cell line and primary hippocampal neuron cultures. The cell line can be differentiated, yielding axonal neurites with presynaptic-like varicosities that have the ability to release acetylcholine via exocytosis in response to stimulation. In addition, this model system does not endogenously express nicotinic receptors, allowing selective exogenous gene sequence expression of the target. The primary hippocampal neuron cultures provide a physiologically relevant model. Non-limiting examples of results are shown in FIGs. 4. 5A-5B. 6A-6B. 7A-7B. 8. 9A-9B. 10A-10B. 13A-13.C and 14A-14B.

|(Kil(W| Neuroblastoma Clonal Cell Culture and Transection. Rodent hybrid neuroblastoma NG108-15 cells were used as a model nerve cell system allowing reconstitution with defined target receptors for Αβ. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 15% fetal bovine serum (FBS) and differentiated in the presence of reduced serum (1% FBS) and 1mM dibutyryi cyclic AMP for 72h. Expression vectors (pcDNA3.1) containing mouse sequences for a4 and β2 nAChR subunits (1:4 ratio respectively) were transiently transfected into the cells using FuGENE HD (ThermoFisher. catalog # PRE2311) for 48h. Mock-transfected NG108-15 cells, exposed only to FuGENE HD and not plasmid DNA. were used as controls.

|OOI09| Hippocampal Neuron Culture. All animal procedures followed an lACUC-approved protocol in accordance with animal welfare guidelines. Hippocampal neuron cultures were prepared from neonatal mouse pups (0-2d old) of either gender (roughly equivalent numbers) obtained from established colonies of wild-type B6.SJL (background) mice. Following rapid decapitation, brains were removed into ice-cold Neurobasal A medium (NB) containing B-27 supplement. 5% fetal bovine serum and Gentamicin (serum NB). Hippocampi were then isolated under a stereomicroscope. The hippocampi were digested with papain (Worthington. catalog # 35N16202) in Hanks with 10mM cysteine at 37"C for 15mins. The preparations were washed by centrifugation in serum NB. The cells were dissociated using sequential trituration with polished Pasteur pipettes of decreasing diameter and collected by low-speed centrifugation. The dissociated cells were preplated in standard tissue culture dishes to remove adherent non-neuronal cells (glia:fibroblasts) for 10-15 mins. The neuron-enriched supernatant was diluted to 1x10 ^s cells/mL and plated into poly-D-lysine-coated 24-well dishes or onto Cell Tak-coated coverslips in serum NB. The cultures were maintained in Neurobasal A medium containing B27 and Gentamicin for 7-10 days prior to treatment.

I (Ml I IU| Ca ²⁺ Imaging. Fluo-4, a Ca ² ' indicator dye. was loaded into cells on coverslips and visualization of [Ca ^2* ' was done via a Nikon PCM 2000 Cameleon AOTF confocal microscope. Each time-series was normalized to baseline fluorescence intensity at time zero (F/FO). After recording a baseline image series, each treatment was perfused over cells for 2.5 minutes or 15 minutes and quantification of relative fluorescence changes was analyzed through ImageJ. Non-Nmiting examples of results are shown in FIG. 13A-13C.

1001 l l| Synaptic Function Assay. Extracellular recording of acutely isolated mouse hippocampal slices was performed to assess changes in synaptic plasticity as measured by changes in long-term potentiation. Slices under perfusion with artificial cerebrospinal fluid (aCSF) were stimulated via the Schaffer collateral axons (HFS: high-frequency stimulation), recording responses in the CA1 pyramidal cell region with or without prior perfusion with the N-terminal Aβt fragment. Non-limiting examples of results are shown in FIG. 11.

1001 121 Cell Stress Assays. Cells were plated, differentiated and treated for 3 days prior to assay. Cell count and fluorescence intensity of reactive oxygen species (ROS)-positive cells were assessed as a means to measure cellular oxidative stress using an Image-IT® LIVE Green Reactive Oxygen Species Detection Kit. In brief, the cells were treated for three days for NG 108-15 cultures and five or ten days for primary hippocampal neuronal cultures. The media was changed every day. thus replenishing with fresh Αβ every 24h. unless otherwise noted. Thereafter, the cells were incubated with carboxy-H.-DCFDA (component A) at 37°C for 30min. During the last 5min of incubation. 2 μg/ml of HOECHST stain (component B) was added to assess, in parallel, the integrity of the cell nuclei. The cells were washed twice with HBS and visualized using an Olympus 1X81 epifluorescence microscope at excitation/emission of 495/529 nm (ROS) and 350/461 nm (HOECHST). respectively. Exemplary results are shown in FIGs. 4. 5A. 6A. 7A-7B. 8. and 9A-9B.

[00113] Cell Survival. Cell survival was determined by counting cells everyday for 7 or 10 days of treatment. For each condition, all cells in 5 random fields of view were counted and averaged. Percent cell death was calculated based on daily cell counts as a proportion of starting cell count numbers (i.e. day 0).

[00114] Cell Death (Apoptosfs) Assay. Cells were plated, differentiated and treated for either 3 or 4 days prior to assay. Apoptotic cells were detected as DNA fragmentation by Click-IT TUNEL Alexa fluor 488. In brief, the cells were treated for four days, exchanging media each day. After the fourth day of treatment, the cells were fixed with freshly prepared 4% paraformaldehyde in PBS at room temperature for 20 min and permeabilized with Triton X-100 (0.25% in PBS) for another 20 min. The cultures were then washed twice and incubated with 50 μL of terminal deoxynudeotidyl transferase reaction buffer (Component A) for 10 min at room temperature. The buffer was replaced with TUNEL reaction mixture containing terminal deoxynucleotidyl transferase and incubated in a humidified chamber at 37 ^U C for 60 min. The cells were then washed three times with 3% BSA in PBS for 2 min each and then incubated with SO ul of Click-iT reaction mixture (containing Alexa 488 azide) for 30 min at room temperature, protected from light exposure. The cells were again washed with 3% BSA in PBS and the eel nuclei were counterstained with Hoechst 33342 for 15 min at room temperature, protected from light. The coverslips were washed twice with PBS before mounting onto a slide with Vectashield mounting medium. Non-limiting examples of results are shown in FIG. 14A-14B.

|imi I5| RESULTS

[IHII I6| The N-Apcore and the Ν-Αβ fragment protect against Mi-length Αβ-induced oxidative stress. 100117| To assess the potential neuroprotective effects of the non-toxic N-ABcore. N-ABcore mutants or N- Αβ fragment against ΑβΙ-42-induced toxicity, these indicators were assessed following administration of the N-terminal peptides under various conditions with daily treatment with 100nM Αβ1-42. Co-treatment with the N-AB fragment at similar or higher concentrations was able to prevent ΑβΙ-42-induced oxidative stress (FIG. 4: p<0.0001 ). Priming (pre-treatment) with the N-AB fragment also significantly reduced Αβ1- 42-induced ROS (FIG. 4; pO.0001 ). The rescue treatment, in which the N-AB fragment was introduced into the culture 1 day after AB1-42. also attenuated ROS to a significant extent (FIG. 4: p<0.0001). In addition, treatment with a cell-permeant antioxidant. N-acetylcysteine (NAC) verified that the Αβ1-42- induced oxidative changes were the result primarily of reactive oxygen species. Turning to the N-ABcore, co-treatment at nM concentrations or post-treatment (rescue) for 3-. 2-, or 1-day reduced ΑβΙ-42-induced ROS back to baseline levels (FIG. 5A: p<0.0001), suggesting that the N-ABcore also protects against of ΑβΙ-42-induced toxicity, including late stages of oxidative stress.

|00l I8| In mock-transfected cells (absent nAChRs). higher concentrations (μΜ) of Αβ1-42 are required to induce significant levels of ROS. Co-treatment with ηΜ-μΜ concentrations N-Aβ core or Ν-Αβ fragment reduced this oxidative stress compared to Αβ1-42 alone (FIG. 6A: p<0.0001). demonstrating that the N- Aβ core or Ν-Αβ fragment can protect against ΑβΙ-42-induced toxicity independently of o4P2-nAChRs. The inactive [H13A.H14A] and highly active [Q15H] NaPcore mutants were further examined on whether they had any protective effect on elevated oxidative stress from Αβ1-42. It was found that co-treatment with nM concentrations of the active mutant reduced ROS compared to Αβ1-42 alone (p<0.001), whereas the inactive mutant had no significant effect (FIG. 9A) at the concentration tested. These results substantiate the specificity of action of the N-ABcore in neuroprotection against ΑβΙ-42-induced oxidative stress.

|0OI I9| N-Aβ core and Ν-Αβ fragment protects against ΑβΙ-42-induced oxidative stress in primary hippocampal neurons.

|U0I2U| The experiments were repeated in primary hippocampal cultures to assess the protective action of Ν-Αβ fragment or Ν-ΑβοοΓβ in a relevant primary neuronal model. Primary mouse hippocampal neuron cultures were either treated daily with 1μΜ Αβ1-42. Ν-Αβ fragment or Ν-Αβ core alone, or co-treated with Αβ1-42 and Ν-Αβ fragment or N-ABcore for 5 days (FIG. 7A) or 10 days (FIG. 7B). As expected, treatment with 1 μΜ Αβ1-42 resulted in 40-50% ROS-positive cells at five days and close to 70% ROS-positive cells at 10 days across the cell population. Co-treatment of cultures with 1μΜ Αβ1-42 and 1μΜ Ν-Αβ fragment or 1μΜ N-Aβ core reduced the levels of ROS down to untreated (baseline) levels when compared to daily treatment with Αβ1-42 alone (p<0.0001). demonstrating that the N-terminal Αβ peptides were also able to fully protect against Αβ-induced oxidative stress in primary neurons.

I (to 1211 Prolonged exposure to N-Aficore or Ν-Αβ fragment protects against ΑβΙ-42-induced apoptosis. I «01221 In addition to inducing oxidative stress, expression of α4β2-nAChRs sensitizes differentiated neuroblastoma NG108-15 cells to Αβ1 -42-induced apoptosis, while elevated Αβ1-42 triggers apoptosis in the absence of nAChRs. Therefore. N-Aβ core or Ν-Αβ fragment were further assessed on whether they can protect against AB1 -42-induced apoptosis with or without the presence of α4β2-nAChRs. Co- treatment with nM concentrations of the N-ABcore in the presence 04p2-nAChRs on day 1. 2 or 3 following the start of daily AB1 -42 treatment reduced apoptosis compared to Αβ1-42 alone (p<0.0001 ), as measured by TUNEL staining for apoptotic cells {FIG. 14A), suggesting that the N-Aβ core can protect against late stages of ΑβΙ-42-induced neuronal death. In addition, high concentrations of Αβ1 -42 induced apoptosis in the absence of the sensitizing o4p2-nAChRs, and co-treatments with ηΜ-μΜ concentrations N-Aβ core or Ν-Αβ fragment over four days also reduced DNA-fragmentation compared to Αβ1-42 alone (ρκθ.0001 for 100nM and 1μΜ N-Aβ core; p<0.001 and p<0.0001. lOOnM and 1μΜ Ν-Αβ fragment, respectively), with a trend of greater reduction at μΜ concentrations (FIG. 14B). This indicates that the N- Αβ∞Γβ or Ν-Αβ fragment can also protect against Αβ1 -42-induced neuronal death independently of the α4β2-nAChRs.

1001231 Ν-Αβεοτβ and Ν-Αβ fragment protect against Αβ1-42 -induced cell death.

[00124] In cell survival assays, the impact of co-treatments with Ν-Αβ fragment or N-Aβ core on Αβ1 -42- induced cell death was assessed for cultures of either differentiated NG108-15 cells or mouse hippocampal neurons over 7- or 10-days through cell counts. As expected, 7-day cell counts in NG108-15 cultures showed significant cell death in α4β2-nAChR-transfected cells treated with Αβ1-42 alone (FIG. 10A). Co-treatment with 100nM N-Aβ core as well as the Ν-Αβ fragment was able to fully protect against ΑβΙ-42-induced cell death at 7 days compared to Αβ1-42 alone (p<0.0001). Similarly, al high concentrations (1μΜ). both Ν-Αβ fragment and Ν-Αβcore protected primary hippocampal neurons against ΑβΙ-42-induced cell death over 10-day treatments compared to Αβ1-42 alone (FIG. 10B; p<0.0001).

[00125] Stabilization of N- Ν-Αβcore retains its receptor-linked Ca ²⁺ activity and protects against Αβ1-42· induced neurotoxicity in α4β2-nAChR-transfected cells.

[00126] To protect the N-Aβ core from exopeptidase degradation in vivo, the N-terminus was capped with an acetyl group and its C-terminus with an amide group. Relative to the N-Aβ core, the capped-N-Aβ core retained its potent activity (FIG. 13B). with a trend towards an increased Ca ⁷⁺ response. To further protect the N-Aβ core from endopeptidase activity, the enantiomeric conversion from the L-configuration to the D- configuration for each amino acid retained activity (FIG. 13B). In addition, the stabilized N-Aβ core. at nM concentrations, was as neuroprotective against AB1 -42-induced oxidative stress as the N-Aβ core (FIG. 9B; p<0.05 vs. Αβ1-42 alone), indicating the potential for use of the stabilized peptide in vivo. |IMII27| The present inventions demonstrates that treatment with the Ν-Αβ fragment or N-Aβ core can effectively and potently protect against Αβ1-42 -induced oxidative stress and apoptosis. Remarkably, treatment with the Ν-Αβ fragment or N-Aβ core after the induction of oxidative stress by Αβ1-42 also rescued the cells from this neurotoxicity. Furthermore, the fragment or N-Aβ core were also protective against neurotoxicity induced by high concentrations of Αβ1-42 in the absence of sensitizing nAChRs, again either with co-treatment or post-treatment rescue. In addition, only high levels (uM) of the Ν-Αβ fragment protected against excitotoxicity in present model neuronal cultures. Together, these findings would suggest that the Ν-Αβ fragment and N-Aβ core may be neuroprotective through altered binding of Αβ1-42 for key target sites and/or activation of alternative pathways, such as an anti-apoptotic pathway and/or anti-oxidative pathway, blocking or reversing the process by which Αβ1-42 induces neurotoxicity.

[00128] As used herein, the term "about" refers to plus or minus 10% of the referenced number.

|IHII29| Various modifications of the invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. Each reference cited in the present application is incorporated herein by reference in its entirety.

|00Uu| Although there has been shown and described the preferred embodiment of the present invention, it will be readily apparent to those skilled in the art that modifications may be made thereto which do not exceed the scope of the appended claims. Therefore, the scope of the invention is only to be limited by the following claims. In some embodiments, the figures presented in this patent application are drawn to scale, including the angles, ratios of dimensions, etc. In some embodiments, the figures are representative only and the claims are not limited by the dimensions of the figures. In some embodiments, descriptions of the inventions described herein using the phrase "comprising ^" includes embodiments that could be described as "consisting of. and as such the written description requirement for claiming one or more embodiments of the present invention using the phrase "consisting of" is met.