Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
NEW BIFUNCTIONAL LIPASE MUTANT AND USES THEREOF IN FLOUR PRODUCT PROCESSING
Document Type and Number:
WIPO Patent Application WO/2015/127596
Kind Code:
A1
Abstract:
Disclosed is a new bifunctional lipase mutant and uses thereof in flour product processing. An amino acid sequence of the mutant has mutations in the form of 1) or 2): 1) P298T; P298T/H317P; P298T/H317P/V326S; P298T/T218S/S234F; P298T/H317P/P168L/A129S; P298T/S234F/K161R/V326S; and 2) a nucleotide sequence with homology over 80% that is obtained through deletion, substitution, insertion or mutation of bases on the basis of a nucleotide sequence defined in 1). The mutant has good application effects in flour product processing. The mutant has a significant whitening effect in processing of flour products such as steamed bun and has an effect of significantly increasing the specific volume in bread baking processing, and therefore has a good application prospect.

Inventors:
XU, Yan (No.1800, Lihu Avenue Binhu Distric, Wuxi Jiangsu 2, 214122, CN)
YU, Xiaowei (No.1800, Lihu Avenue Binhu Distric, Wuxi Jiangsu 2, 214122, CN)
Application Number:
CN2014/072549
Publication Date:
September 03, 2015
Filing Date:
February 26, 2014
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
JIANGNAN UNIVERSITY (No.1800, Lihu Avenue Binhu Distric, Wuxi Jiangsu 2, 214122, CN)
International Classes:
C12N9/20; A21D8/04; C12N1/00; C12N1/19; C12N15/63
Foreign References:
CN101974499A2011-02-16
CN102160562B2012-12-05
CN101974499B2012-07-11
Other References:
WANG, RUI ET AL.: "Increasing Activity of Rhizopus chinensis CCTCC M201021 lipase by directed evolution-error prone PCR", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 25, no. 12, 25 December 2009 (2009-12-25), XP055258943
See also references of EP 2933330A4
YU XIAO-WEI ET AL., J MOL CATAL B: ENZYM, vol. 57, 2009, pages 304 - 311
YU XIAO-WEI ET AL., MICROBIAL CELL FACTORIES, vol. 11, 2012, pages 102 - 112
WANG LELE ET AL.: "Cloning and expression of pro- and mature Rhizopus chinensis lipase in Pichia pastoris", THE HIGH-TECH COMMUNICATIONS, vol. 19, no. 10, 2009, pages 105
Attorney, Agent or Firm:
BEIJING AIPUNAJIE IP FIRM (Room 208, No.9 XinQiWang BuildingNo.1 DongDaDi Street, Dongcheng District, Beijing 2, 100062, CN)
Download PDF: