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Document Type and Number:
WIPO Patent Application WO/1997/016405
Kind Code:
Compounds, or their compositions, of general formula: A - X1 - NO2 or their salts, used as medicaments, wherein: A = R(COX)t; t = 0 or 1; X = O; X0 = X; R being (a) wherein: R1, R2 and R3 are alkyls; X1 is a bivalent linking group chosen among -YO wherein Y is chosen among a linear or when permissible branched C1-C20 alkylene, a cycloalkylene having 5 to 7 carbon atoms optionally substituted; (b) wherein n is an integer from 1 to 6, R2a being H, CH3; r = 0 or 1.

Del, Soldato
Piero, Sannicolo'
Application Number:
Publication Date:
May 09, 1997
Filing Date:
October 29, 1996
Export Citation:
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Nicox S.
Del Soldato, Piero Sannicolo' Francesco
International Classes:
A61K31/60; A61K31/00; A61K31/165; A61K31/21; A61K31/215; A61K31/216; A61K31/33; A61K31/36; A61K31/618; A61P7/00; A61P7/02; A61P29/00; A61P31/00; C07C203/04; C07C219/34; C07C235/44; C07C235/56; C07C235/64; C07D317/62; (IPC1-7): C07C203/04; C07C235/44; A61K31/21; A61K31/165
Domestic Patent References:
Other References:
LECHI C ET AL: "In vitro study of the anti-aggregating activity of two nitro derivatives of acetylsalicylic acid", BLOOD COAGULATION FIBRINOLYSIS (BLFIE7,09575235);96; VOL.7 (2); PP.206-209, UNIVERSITY VERONA;INSTITUTE CLINICAL CHEMISTRY; VERONA; 37134; ITALY (IT), XP000614646
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1. Compounds, or their compositions, of general formula. A X_ N02 or their salts, used as medicaments, wherein: A = R(COX)t, t = 0 or 1; X = 0, NH, NRlc wherein Rlc is a linear or branched, when possible, alkyl from l to 10 C ato¬ ms, preferably from 1 to 4 or !CH2CHCH20)m (CH.CH0) n ONO ς2a wherein m and n are integer from 1 to 6; R2a being H, CH3, the linking with Xχ can be in any position of the ring; OCOR3 is preferably in ortho posi¬ tion with respect to C0XQ; = X; R is chosen among : wherein : Rχ is the group OCOR3; wherein R3 is methyl, ethyl or linear or branched alkyl C3C5, or the residue of an etherocycle with only one ring having 5 or 6 atoms which can be aromatic, partially or completely hydroge¬ nated, containing one or more etheroatoms selected independently among 0, N and S; R2 is hydrogen, idroxy, halogen, linear or when permis¬ sible branched C1C4 alkyl, linear or when permissible branched alcoxyl from 1 to 4 carbon atomε; a linear or when permissible branched pefluoroalkyl having from l to 4 carbon atoms, nitro, ammino, mono or dialkylami¬ ne with C1C4 alkyl; Rj_ and R2 together are a dioxymethylene group, with the proviεo that when X = NH,then Xλ iε ethylene and R2 = H; R cannot be 0C0R3, in position.
2. when R3 is methyl; nl being an integer 0 or 1; Xλ in the formula AX1N02, is a bivalent connecting bridge chosen from the following: YO where Y is selected from: a linear or when permisεible branched C1 C2 Q alkylene, a cycloalkylene having from 5 to 7 carbon atoms optio¬ nally substituted; (CH2 CH0) n (Y0) r R2 wherein n iε an integer from l to 6, R2a as defined above; r = 0 or l . Compounds or their compositions according to claim l, having general formula: 3 Compounds or their compositions according to claims 1, 2 having general formula: wherein^ , is CK2ON02, CH2CK2CH2CH20N02, CH2CH2ON02, CH2CH20CH2CH20N02, CH2CH20CH20N02. Use of the compounds, or their compositionε according to claimε 1 to 3 for the preparation of medicamentε for the septic shock. Use of the compounds, or their compositionε according to claims 1 to 3 for the preparation of medicaments for antiinflammatory and antithrombotic products. 6. Compounds, or their compositions according to claims 1 to 3.


The present invention relates to new products having anti-inflammatory, analgesic and anti-thrombotic activities.

In particular it relates to inhibitors of cyclo-oxyge- nase (COX) of the class of Aspirin, i.e. of acetylsalicidic acid or its derivatives in general.

It is known that the anti-inflammatory and anti-throm¬ botic efficacy of NSAIDs (Non Steroid Anti-Inflammatory Drugs) , also known as FANS, but above all their tolerance, seem to be markedly affected by their inhibitor activity of the cyclo-oxygenase (COX) in the inflammatory site as well as in healthy tissue. See for example FASEB Journal l, 89, 1987; Bioch. Biophys. Acta 1083, 1, 1991. It is generally believed that the stronger a COX inhibitor iε the more effe¬ ctive it is.

The disadvantage of these products is that they are toxic.

Furthermore, it is also known that the COX-inhibiting properties seem to depend on some factors bound to the physico-chemical and structural characteristics of the mole¬ cules themselves, such as for example the acidic function. See for example J. Pharmacol. Exp. Therap. 196, 226, 1976; Arch. Toxicol. 60, 261, 1987.

The known cyclo-oxygenase inhibitors are generally acids which can be brought back to general structures, in¬ cluding: carboxyl acids, either acetylated such as, for example, aspirin and triflusal, or nonacetylated such as, for example, salycilate, diflunisal, salsalate; acetic acids, for example diclofenac, indomethacin, tolmetin, sulindac, etodolac, ketorolac,- propionic acids, such as, for instance, ibuprofen, na¬ proxen, pirprofen, tiaprofenic acid, loxoprofen, indo- profen, oxaprozin, ketoprofen, fenoprofen, fenbufen, flurbiprofen, carprofen, suprofen.

See for example a previous patent application in the name of the applicant PCT/EP 95/01233, herein incorportated by reference, which describes the prior art of the above products.

As said, the disadvantage of these products is that they are very effective but highly toxic.

The importance of the acidic function resides in the fact that the masking of this function in COX inhibitors re¬ sults in a virtually complete loss of its prostanoid-inhibi- ting properties. See Drugs 35, 504, 1988.

Products are also known which are highly effective in inhibiting cyclooxygenase and have a low toxicity even though they do not contain the acidic function in their mo-


These products are known as nitric esters with nonaci die ending. See for example patents WO 94/04484, which describes a particular group of compounds including the well known commercial product diclofenac; WO 94/12463, which de¬ scribes another specific group of compounds including the commercial products flurbiprofen and indoprofen, PCT/EP 94/03182, which describes another specific group of com¬ pounds including the commercial products naproxen and keto- rolac.

In a previous patent application in the name of the applicant PCT/EP 95/01233 other nitric esters having a nona- cidic termination have been described with various linking groups X-_ as specified below.

The new linking groups therein described showed advantages from the pharamcological and pharmaceutical viewpoint, in particular pharmaco-cinetic and pharmaco-dynamic viewpoint, since they showed a lower variability of the response. The products described in said patent application were also able to exert an inhibition effect of the inflammation produced by lyposaccaride (LPS) and therefore useful in the septic shock. This result was unexpected since it is well known that the anti-inflammatory products in general do not signi¬ ficantly modify the activity of the nitrosynthetase induced by lypopolysaccarides in the rat and therefore they are not

useful in the septic shock.

The technical problem to be solved by the present in¬ vention relates to inhibitor products of the COX much more effective in inhibiting the piastrine aggregation induced by arachidonic acid and trombin, the latter having a well known primary patogenetic role even superior to arachidonic acid and other aggregant stimulus, said products having contempo¬ raneously a high gastric tolerability, without provoking adhesions of the gastric intestinal mucose on the treated animals.

The applicant has unexpectedly and surprisingly found a specific class of anti-inflammatory products, as described hereinbelow, having an improved inhibtor activity of the COX combined with a low toxicity.

An object of the present invention are compounds, or their compositions, of general formula:

A-X 1 -N0 2 or their salts, for use as medicaments, in particular as anti-inflammatory and anti hrombotic agents, having improved efficiency in inhibiting the piastrinic aggregation induced by arachidonic acid and/or trombin, wherein: A = R(COX) t , wherein t is zero or 1;

X = 0, NH, NR 1C wherein R 1C is a linear or branched alkyl ha¬ ving l to 10 C atoms, preferably 1-4 C atoms, or

) n ,

wherein m and n are integers from l to 6, preferably m from 1 to 3 , and n from 2 to 4; R 2a being H, CH 3 ; the linking with X 2 can be in any position of the ring, preferably in position 2; -OCOR 3 preferably in position ortho with respect to-C0X 0 -;

X 0 = X;

R is chosen from:


R α is an OCOR 3 group, wherein R 3 is methyl, ethyl or a linear or branched 2 - 5 alkyl, or the residue of a heterocycle with a single ring having 5 or 6 atoms which may be aromatic, partially or totally hydrogenated, containing one or more heteroatoms independently chosen from 0, N, and S; R 2 is hydrogen, hydroxy, halogen, a linear or when permissi¬ ble branched alkyl having l to 4 C atoms, a linear or when permissible branched alkoxyl having l to 4 C atoms, a linear or when permissible branched perfluoroalkyl having 1 to 4 C atoms, for example trifluoromethyl; nitro, amino, mono- or

di-alkylamine in which the alkylamine has l to 4 C atoms; R χ and R 2 together are a dioxymethylene group, with the pro¬ viso that when X = NH, then X is ethylene and R 2 = H; R χ cannot be OCOR 3 in position 2 when R 3 is methyl; nl being 0 or 1;

X-_ in the formula A-X 1 -N0 2 is a bivalent connecting bridge chosen from the following:

-YO- where Y is selected from: a linear or when permissible branched C 1 -C 20 alkylene, preferably having from l to 3 carbon atoms,- a cycloalkylene having from 5 to 7 carbon atoms optio¬ nally substituted; - (CH 2 -CH-0) n - (Y0) r -

R 2a wherein n is an integer from 1 to 6, preferably from 2 to 4; R 2a as defined above; r = 0 or l,- Y as defined above, preferably C 1 -C 10 , preferably C 2 -C 6 . The preferred products according to the present inven¬ tion are those in which t = 0, X Q is oxygen,- the group having N0 2 is in position 2 with respect to -COX 0 ; nl = 0; R 3 = CH 3 . In particular the preferred products according to the pre¬ sent invention are the following:

wherein ! - is -CH 2 -ON0 2 , -CH 2 CH 2 CH 2 CH 2 ON0 2 , -CH 2 CH 2 ON0 2 , -CH 2 CH 2 0CH 2 CH 2 0N0 2 , -CH 2 CH 2 0CH 2 0N0 2 .

The processes for obtaining compounds of the invention are for exapmle those listed in The Merck Index, XI ed. , 1989, page 16, n. 95 or in the German Patent 236.196 or by methods well known to the chemist for the introduction of the groups in the various positions. The modifications of the compounds of the general formula can be obtained by using the processes cited in the patent WO 92/01668.

The products of the present invention of general formu¬ la A-X 1 -N0 2 with the linking groups X χ as defined above, are obtainable by using the methods of the prior art described above or by modifying the known methods for the introduction of the linking group X χ when these are different from the linking groups stated in the cited patents .

The same is valid also for the introduction of the -COX 0 - group.

Generally, the connection between A and X χ is, as seen, generally, of the ester or amidic type (NH or NR 1C , as defi-

ned in X) . All well known synthetic routes for forming these bonds may be used to form this connection.

In the case of the esters, the most direct synthetic route involves a reaction of acyl chlorides R-CO-Cl with halogen alcohols for example HO-Y-C1, HO-Y-Br, HO-Y-I, in the experimental conditions well known in the art.

The reaction products are converted into the final pro¬ ducts by reacting with AgN0 3 in acetonitrile, in accordance to what known from the literature.

The general route is as follows:

R-CO-Cl + HO -Y- Br ^ R-CO-O-Y-Br + AgN0 3 >

A-X 1 -N0 2 wherein X χ = YO.

In the case of amides the synthetic route involves a reaction of said acyl chlorides RCOC1 with amino alcohols of the general formula NH 2 -Y-0H, NHR 1C -Y-0H to give amides of the general formula: R-CO-NH-Y-OH and R-CO-NR lC -Y-OH in accordance with known methods.

The reaction of said amides with halogenating agents such as, for example, PC1 5 , PBr 3 , S0C1 2 , etc., brings to ha¬ logen derivatives of the general formula: R-CO-NH-Y-Br(Cl) and R-C0-NR 1C -Y-Br(Cl) .

The latter products by reacting with AgN0 3 in acetoni¬ trile in accordance with known literature methods, bring to the final products A-X χ -N0 2 .

The route may be outlined as follows:

PBr 3

R-CO-Cl + NHR lc -Y-OH ^ R-C0-NR 1C -Y-0H )

R-C0-NR 1C -Y-C1 + AgN0 3 R-CO-NR χc -Y-ON0 2 wherein YO is X χ .

An alternative route to form the esters is a reaction of the sodium or potassium salts of the acids with the nitric esters of halogen alcohols of the general formula:

N0 2 -0-Y-Cl(Br,I) to directly give the products of the invention.

The reaction route is as follows:

R-CO-ONa+Br-Y-ON0 2 R-CO-0-Y-ON0 2 wherein YO is X χ .

The following examples are being given only as illu¬ strative explanation but not as a limitation of the present invention. EXAMPLES Example 1: Comparison - Preparation of the products

It was used acetylsalicilic acid ASA available on the market, Aspirin of Bayer.

Example 2: Comparison - Preparation of the compound A-X 1 -N0 2 , wherein R has the formula below of Aspirin, X χ is -(CH 2 ) 4 0-, herein called ANBE, and having general formula: 2-acetoxy-benzoate of (4-nitroxy)butyl

Preparation of the intermediate having formula: 2-acetoxy-benzoate of (4-bromine)butyl

COO(CH, , Bf


At a solution of: acetylsalicilic acid 15.0 g and dimethylformamide 50 ml kept at 0°C under nitrogen stream it is added portionwise:

2.6 g of Nal (80% by weight suspension in vaseline oil) .

The mixture was left under stirring for l hour and then was dropped in 5 hours, at 25°C in a stirred solution of:

2,2' -dibromo-butane 27.0 g and dimethylformamide 50 ml

The mixture was left under stirring for 3 days, then waε dried at reduced pressure. The residue was treated with: water 50 ml dichloromethane 50 ml

The phases were εeparated and the aqueouε phase was further extracted in 10 ml of dichloromethane.

The pooled organic phaεes were washed with water ( 3 x 25

ml) , dried (MgS0 4 ) , decoloured with animal charcoal (1 g) , and brought to dryness in vacuum.

The residue (26.0 g) was used crude for the next reaction. Preparation of ANBE At a solution of

ASA- (CH 2 ) 4 Br 26.0 g acetonitrile 65 ml kept at room temperature and sheltered from light, was added silver nitrate 21.0 g

After 2 days under stirring were added 4.3 g of silver ni¬ trate.

After 2 further dayε under the εame conditions the inεoluble εaltε were filtered and the filtrate waε freed of the εol- vent at reduced pressure.

A residue of 18.0 g was obtained and the chromatography on a silica gel column (500 g of silica) eluting with a to¬ luol/ethyl acetate 95/5 v/v mixture was carried out. The fractions resulted uniform for TLC (Thin Layer Chromato¬ graphy) analysis and were pooled and brought to drynesε and gave 15.0 g of ANBE.

The X H NMR (CDC1 3 ) (80 MHz) analysis showed the following data:

2.28 (3H, S) ; 1.2 (4H, m) ; 4.30 (2H, t) ; 4.50 (2H, t) ; 7.3 (3H, m) ; 7.95 (IH, dd) . The IR analysis (Nujol) provided the following results:

υ oco = 178 ° cm_1 ; "coo = 1725 cm "1 ,- v 0N02 = 1641 e 1287 cm "1 . Mass spectrometry gave a molecular weight value of 297. EXAMPLE 3 - Preparation of the compound A-X 1 -N0 2 wherein R has the formula below, X is -(CH 2 )0, herein called ANMPE ha¬ ving formula: 2-acetoxy-benzoate of (3-nitroxymetyhyl) fenyl

Preparation of the intermediate of formula

In a l 1 flask were added: 3-OH-benzyl alcohol 28.1 g (0.226 mols; Methylene chloride 85 ml HBr (48% by weight in water) 140 ml and were kept under stirring at room temperature for l hour and half .

At the end the phases were separated and the aqueous phase was further extracted with methylene chloride (about 50 ml) . The pooled organic phases were washed twice with: Distilled water 100 ml

Solution of NaHC0 3 at 5% (w/v) 50 ml

Then it was anhydrified on MgS0 4 and was brought to dryness obtaining a residue equal to 34.13 g of crystalline solid. The product was characterized by TLC analysis, by using a toluol/ethylacetate 7/3 v/v mixture as eluent. The so obtained product is used immediately for the follo¬ wing reaction.

In a 1 1 flack provided with stirrer, termometer, drop¬ ping syεtem were added:

Previouε reaction residue 34 g

Acetonitrile 100 ml

In the dropping syεtem it waε charged a solution of: Silver nitrate 38.5 g

Acetonitrile 60 ml and it waε dropped in about 2 hourε, keeping the flask εhel- tered from light and cooling on a water bath. The temperature waε maintained between 20 and 30°C. It waε left to react for about 15 hours.

Then it was filtered and the filtrate was dried; at the re¬ sidue was added etylacetate, about 500 ml, then silica (50 g) and coal (3 g) .


The filtrate was dried again and a charomatography on about

300 g of silica using toluol as eluent was carried out by using the chromatographic system indicated above.

11.7 g of product were obtained (dark oil) and chatacterized by TLC.

Preparation of ANMPE

In a 250 ml flask provided with stirrer, termometer, drppoing system were introduced: 3-hydroxybenzylnitrate 4.95 g potassium carbonate 7.0 g etylacetate 50 ml

It waε cooled at 0°C and dropped under nitrogen εtream in 15 minutes a solution of: acetylεaliciloil chloride 5.01 g ethylacetate 20 ml

At the end of the dropping it waε left to react for about 4 hourε at 20°C.

The reaction waε made in TLC (toluol ethylacetate 9/1 v/v) . At the end 70 ml of distilled water was added. The phases were separated, the aqeuous phase was extracted again with 30 ml of ethylacetate and the pooled organic pha-

ses were washed with water (30 ml) containing sodium chlori¬ de (10 g) .

The organic phases were then anhydrified on magnesium sul¬ phate and dried; a 8.9 g residue was obtained (yellow oil) which solidifies for cooling at 0°C. By crystallization from isopropilic ether 6.5 g of ANMPE were obtained at the pure state. The -"-H NMR (CDC1 3 ) (80 MHz) analysis gave the follo¬ wing data.-

2.34 (3H, S) ; 5.45 (2H, s) ; 7.05-7.75 (7H, m) ; 8.24 (IH, dd) . EXAMPLE 4: pharmacological exampleε

The products prepared above were characterized by a pharmacological viewpoint.

In the in vivo studies (for example toxicity) the products obtained above were administered in form of suεpension in carboxymethylcellulose 1-2% by weight.

For the in vitro testε (piaεtrinic tests) the nitroderivati- ves, 1 mmol, were disεolved in dimethylεulfoxide and then diluted according to the concentrations liεted in the Table. Aspirin ASA 30 mmols waε dissolved in a mixture of etanol:H 2 0 in the range 1:10 by volume and then diluted according to the concentrations listed in the Table.

The samples, obtained without adding the substance under examination (ASA, ANBE, ANMPE) , did not show any significant reply.


The acute toxicity was evaluated through oral submini- stration of a single dose of 1, 3, 10, 30, 100, 200 mg/Kg of product in groups of 10 little rats.

The lethality incidence and the appearance of toxic sintho- matology were noted within a period of 14 days. Also after adminiεtration of a dose of 200 mg/Kg the animals did not show any apparent toxicity both with ANMPA and with ANBE. Tolerability

The gastric tolerability was evaluated through oral subministration in the rat measuring the seriousness of the gastropathie induced according to the criterium indicated by Wallace et al . (Am. J. Physiol. 259, G642, 1990) . Piastrinic testε

Anti-aggregating piastrinic activity (anti-thrombotic activity)

The anti-aggregating piastrinic activity waε evaluated in vitro on human piastrines stimulated by trombin or by arachidonic acid according to the method described by Berte- le et al . (Science 220, 517, 1983) .

COX inhibition (anti-inflammatory activity)

The inhibition activity of the cyclooxygenases was de¬ termined in human piaεtrineε according to the method deεcri¬ bed by Patrono et al (Thrombosis Res. 17, 317, 1980) . The enzimatic activity was expressed as level of Tromboxan B2

(T X B2) and measured in ng/ml.

Piastrinic adhesion

The inhibition activity of the piastrinic adhesion was evaluated according to the method described by Bellavite et al. (Anal. Biochem. 216, 444, 1994).

Intracellular piastrinic calcium

The effect of the compounds of the invention or compa¬ rison compounds on the calcium concentration inside the pia- εtrine waε meaεured according to the method of Pollock et al. (Biochem. J. 235, 869, 1986).


From the results of the Table, it can be seen that ANMPE (compound of the invention) is much more efficient with respect to ASA and ANBE in the inhibition of the pia¬ strinic aggregation induced by arachidonic acid. In the case of ANMPE it is higher than ANBE and εimilar to ASA.

Nevertheless in the piastrinic aggregation induced by trombin, which higher patogenetic value is known with re¬ spect to the arachidonic acid or other aggregating stimuluε, the ANMPE giveε values εurpriεingly higher both with reεpect to ANBE and ASA.

For the COX inhibition propertieε, the product of the invention ANMPE εhowε activitieε similar to ASA, but well higher with respect to ANBE.

This is much more surpriεing if we conεider that ANMPE aε well as ANBE, but differently from ASA, it is very well tolerated in the gastric mucose.

Indeed the gaεtric tolerance teεts have shown that al¬ ready at doses of 50-100 mg/Kg ASA induced severe damages in the intestinal gastric mucose of the treated animals. On the contrary ANMPE and ANBE, also when administered at doses of 250-500 mg/Kg did not produce relevant damages.

As regards the other piastrinic tests: piastrinic adhesion and intracellular piastrinic calcium, only ANMPE resulted efficient in inhibiting significantly

and in a dose-dependent way (from 10 "5 to io -4 M) both patho¬ logical processes.

On the contrary it was not possible to see any inhibiting effect with other compounds under examination.