Title:
NEW DIBASIC ORGANIC ACID PRODUCING STRAIN AND PREPARATION AND APPLICATION OF SAME
Document Type and Number:
WIPO Patent Application WO/2016/127920
Kind Code:
A1
Abstract:
Provided are an engineering strain for synthesizing a dibasic organic acid and preparation and application of same. The engineering strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid; as compared with the origin strain of the engineering strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate transaminase, glutamic-aspartate transport protein, C4-dicarboxylic transport protein, pyruvate carboxylase, malate dehydrogenase, and glucose transport protein; the expression product of the negative regulator gene comprises succinyl coenzyme A synthase and malic-α-ketoglutaric transport protein; and the origin strain comprises myceliophthora thermophila, thielavia terrestris, aspergillus, and rhizopus.
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Inventors:
TIAN CHAOGUANG (CN)
LI JINGEN (CN)
LONG CHUANNAN (CN)
SUN TAO (CN)
LIN LIANGCAI (CN)
XU JING (CN)
LIU QIAN (CN)
JI JINGXIAO (CN)
SUN WENLIANG (CN)
MA YANHE (CN)
LI JINGEN (CN)
LONG CHUANNAN (CN)
SUN TAO (CN)
LIN LIANGCAI (CN)
XU JING (CN)
LIU QIAN (CN)
JI JINGXIAO (CN)
SUN WENLIANG (CN)
MA YANHE (CN)
Application Number:
PCT/CN2016/073573
Publication Date:
August 18, 2016
Filing Date:
February 04, 2016
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N1/15; C07K14/37; C12N9/00; C12N9/04; C12N9/10; C12N15/52; C12N15/80; C12P7/44; C12P7/46; C12P7/50; C12R1/645
Foreign References:
CN102947458A | 2013-02-27 | |||
CN102597224A | 2012-07-18 | |||
CN104844698A | 2015-08-19 |
Other References:
DATABASE Genbank 4 January 2012 (2012-01-04), BERKA, R.M. ET AL., XP055474934, Database accession no. XP_003666955
DATABASE Genbank 4 January 2012 (2012-01-04), BERKA, R.M. ET AL., XP003660869, Database accession no. XP 003660869
DATABASE Genbank 4 January 2012 (2012-01-04), BERKA, R.M. ET AL., XP055474940, Database accession no. XP_003662692
DATABASE Genbank 14 November 2006 (2006-11-14), "German Neurospora genome project", XP055474942, Database accession no. CAD21508
See also references of EP 3257934A4
DATABASE Genbank 4 January 2012 (2012-01-04), BERKA, R.M. ET AL., XP003660869, Database accession no. XP 003660869
DATABASE Genbank 4 January 2012 (2012-01-04), BERKA, R.M. ET AL., XP055474940, Database accession no. XP_003662692
DATABASE Genbank 14 November 2006 (2006-11-14), "German Neurospora genome project", XP055474942, Database accession no. CAD21508
See also references of EP 3257934A4
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
上海一平知识产权代理有限公司 (CN)
上海一平知识产权代理有限公司 (CN)
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