NEW DIHYDROQUINOLINE PYRAZOLYL COMPOUNDS AS ALDOSTERONE SYNTHASE INHIBITORS

The present invention relates to organic compounds useful for therapy or prophyl in a mammal, and in particular to aldosterone synthase inhibitors for the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom.

The present invention provides novel compounds of formula (I)

wherein

R ¹ , R ⁵ , R ⁶ , R ⁷ and R ⁸ are independently selected from H, alkyl, haloalkyl, cycloalkyl and halocycloalkyl;

R 2", R 3 ^J and R 4" are independently selected from H, halogen, alkyl, haloalkyl,

cycloalkyl and halocycloalkyl;

R 12 , R 13 , R I ¹ V ^; and R 1 ¹ 6 ^D are independently selected from H, alkyl and cycloalkyl;

R ¹⁰ is alkyl, aryl, substituted aryl, arylalkyl, substituted arylalkyl, heteroaryl,

substituted heteroaryl, hetroarylalkyl or substituted heteroarylalkyl, wherein substituted aryl, substituted arylalkyl, substituted heteroaryl and substituted heteroarylalkyl are substituted with one to three substituents selected from alkyl, halogen, haloalkyl, cycloalkyl, halocycloalkyl, cyano, alkoxy, haloalkoxy, alkylsulfanyl, haloalkylsulfanyl, alkylsulfonyl and haloalkylsulfonyl;

R ¹¹ and R ¹⁴ together form -CH ₂ -CH ₂ -, -CH ₂ (CH ₃ )-CH ₂ - or -CH ₂ -CH ₂ (CH ₃ )-; or R ¹¹ and R ¹² together form -CH ₂ - and R ¹³ and R ¹⁴ together form -CH ₂ -;

A is -C(O)- or -S(0) ₂ - m is 1;

and pharmaceutically acceptable salts thereof.

Herein we describe inhibitors of aldosterone synthase that have the potential to protect from organ/ tissue damage caused by an absolute or relative excess of aldosterone. Hypertension affects about 20% of the adult population in developed countries. In persons 60 years and older, this percentage increases to above 60%. Hypertensive subjects display an increased risk of other physiological complications including stroke, myocardial infarction, atrial fibrillation, heart failure, peripheral vascular disease and renal

impairment. The renin angiotensin aldosterone system is a pathway that has been linked to hypertension, volume and salt balance and more recently to contribute directly to end organ damage in advanced stages of heart failure or kidney disease. ACE inhibitors and angiotensin receptor blockers (ARBs) are successfully used to improve duration and quality of life of patients. These drugs are not yielding maximum protection. In a relatively large number of patients ACE and ARB's lead to so-called aldosterone breakthrough, a phenomenon where aldosterone levels, after a first initial decline, return to pathological levels. It has been demonstrated that the deleterious consequences of inappropriately increased aldosterone levels (in relation to salt intake/levels) can be minimized by aldosterone blockade with mineralocorticoid receptor antagonists. A direct inhibition of aldosterone synthesis is expected to provide even better protection as it will also reduce non- genomic effects of aldosterone as well.

The effects of aldosterone on Na/K transport lead to increased re-absorption of sodium and water and the secretion of potassium in the kidneys. Overall this results in increased blood volume and, therefore, increased blood pressure. Beyond its role in the regulation of renal sodium re-absorption aldosterone can exert deleterious effects on the kidney, the heart and the vascular system especially in a "high sodium" context. It has been shown that under such conditions aldosterone leads to increased oxidative stress which ultimately may contribute to organ damage. Infusion of aldosterone into renally compromised rats (either by high salt treatment or by unilaterally nephrectomy) induces a wide array of injuries to the kidney including glomerular expansion, podocyte injury, interstitial inflammation, mesangial cell proliferation and fibrosis reflected by proteinuria. More specifically aldosterone was shown to increase the expression of the adhesion molecule ICAM- 1 in the kidney. ICAM- 1 is critically involved in glomerular

inflammation. Similarly, aldosterone was shown to increase the expression of

inflammatory cytokines, such as interleukin IL-lb and IL-6, MCP-1 and osteopontin. On a cellular level it was demonstrated that in vascular fibroblasts aldosterone increased the expression of type I collagen mRNA, a mediator of fibrosis. Aldosterone also stimulates type IV collagen accumulation in rat mesangial cells and induces plasminogen activator inhibitor-1 (PAI-1) expression in smooth muscle cells. In summary aldosterone has emerged as a key hormone involved in renal damage. Aldosterone plays an equally important role in mediating cardiovascular risk.

There is ample preclinical evidence that MR-antagonists (spironolactone and eplerenone) improve blood pressure, cardiac and renal function in various pre-clinical models.

More recently preclinical studies highlight the important contribution of CYPl 1B2 to cardiovascular and renal morbidity and mortality. The CYPl 1B2 inhibitor FAD286 and the MR antagonist spironolactone were evaluated in a rat model of chronic kidney disease (high angiotensin II exposure; high salt and uni-nephrectomy). Angiotensin II and high salt treatment caused albuminuria, azotemia, renovascular hypertrophy, glomerular injury, increased PAI-1, and osteopontin mRNA expression, as well as tubulointerstitial fibrosis. Both drugs prevented these renal effects and attenuated cardiac and aortic medial hypertrophy. Following 4 weeks of treatment with FAD286, plasma aldosterone was reduced, whereas spironolactone increased aldosterone at 4 and 8 weeks of treatment. Similarly only spironolactone but not FAD286 enhanced angiotensin II and salt-stimulated PAI-1 mRNA expression in the aorta and the heart. In other studies the CYPl 1B2 inhibitor FAD286 improved blood pressure and cardiovascular function and structure in rats with experimental heart failure. In the same studies FAD286 was shown to improve kidney function and morphology.

Administration of an orally active CYP11B2 inhibitor, LCI699, to patients with primary aldosteronism, lead to the conclusion that it effectively inhibits CYP11B2 in patients with primary aldosteronism resulting in significantly lower circulating aldosterone levels and that it corrected the hypokalemia and mildly decreased blood pressure. The effects on the glucocorticoid axis were consistent with a poor selectivity of the compound and a latent inhibition of Cortisol synthesis. Taken together these data support the concept that a CYP11B2 inhibitor can lower inappropriately high aldosterone levels. Achieving good selectivity against CYP11B1 is important to be free of undesired side effects on the HPA axis and will differentiate different CYP11B2 inhibitors.

The compounds of the present invention according formula (I) are potent inhibitors of CYPB11B2 and present an improved selectivity towards CYP11B2 versus CYP11B1 combined with an improved metabolic stability.

Objects of the present invention are the compounds of formula (I) and their aforementioned salts and esters and their use as therapeutically active substances, a process for the manufacture of the said compounds, intermediates, pharmaceutical compositions, medicaments containing the said compounds, their pharmaceutically acceptable salts or esters, the use of the said compounds, salts or esters for the treatment or prophylaxis of illnesses, especially in the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom and the use of the said compounds, salts or esters for the production of medicaments for the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom.

The term "alkoxy" denotes a group of the formula R'-O-, wherein R' is an alkyl group. Examples of alkoxy group include methoxy, ethoxy, n-propoxy, isopropoxy, n- butoxy, isobutoxy and tert-butoxy. Particular alkoxy group include methoxy.

The term "alkyl" denotes a monovalent linear or branched saturated hydrocarbon group of 1 to 12 carbon atoms. In particular embodiments, alkyl has 1 to 7 carbon atoms, and in more particular embodiments 1 to 4 carbon atoms. Examples of alkyl include methyl, ethyl, propyl and isopropyl, n-butyl, iso-butyl, sec -butyl and ter-butyl. Particular alkyl groups are methyl and ethyl.

The term "alkylsulfanyl" denotes a group of the formula R'-S-, wherein R' is an alkyl group. Examples of alkylsulfanyl are groups wherein R' is methyl, ethyl, propyl and isopropyl, n-butyl, iso-butyl, sec-butyl and ter-butyl. Particular alkylsulfanyl is group wherein R' is methyl.

The term "alkylsulfonyl" denotes a group of the formula R'-S(0)2-, wherein R' is an alkyl group. Examples of alkylsulfonyl are groups wherein R' is methyl, ethyl, propyl and isopropyl, n-butyl, iso-butyl, sec-butyl and ter-butyl. Particular alkylsulfonyl is group wherein R' is methyl.

The term "aryl" denotes a monovalent aromatic carbocyclic mono- or bicyclic ring system comprising 6 to 10 carbon ring atoms. Examples of aryl moieties include phenyl and naphthyl. Particular aryl group is phenyl. The term "arylalkyl" denotes an alkyl group wherein one of the hydrogen atoms of the alkyl group has been replaced an aryl group. Particular arylalkyl group is

phenylmethyl.

The term "cyano" denotes a -C≡N group.

The term "cycloalkyl" denotes a monovalent saturated monocyclic hydrocarbon group of 3 to 10 ring carbon atoms. In particular embodiments, cycloalkyl denotes a monovalent saturated monocyclic hydrocarbon group of 3 to 8 ring carbon atoms.

Examples for cycloalkyl are cyclopropyl, cyclobutanyl, cyclopentyl, cyclohexyl or cycloheptyl. Particular cycloalkyl group is cyclopropyl.

The term "haloalkoxy" denotes an alkoxy group wherein at least one of the hydrogen atoms of the alkoxy group has been replaced by same or different halogen atoms. The term "perhaloalkoxy" denotes an alkoxy group where all hydrogen atoms of the alkoxy group have been replaced by the same or different halogen atoms. Examples of haloalkoxy include fluoromethoxy, difluoromethoxy, trifluoromethoxy, trifluoroethoxy,

trifluoromethylethoxy, trifluorodimethylethoxy and pentafluoroethoxy.

The term "haloalkyl" denotes an alkyl group wherein at least one of the hydrogen atoms of the alkyl group has been replaced by the same or different halogen atoms. The term "perhaloalkyl" denotes an alkyl group where all hydrogen atoms of the alkyl group have been replaced by the same or different halogen atoms. Examples of haloalkyl include fluoromethyl, difluoromethyl, trifluoromethyl, trifluoroethyl, trifluoromethylethyl and pentafluoroethyl. Particular haloalkyl group is trifluoromethyl.

The term "haloalkylsulfanyl" denotes a group of the formula -S-R', wherein R' is a haloalkyl group. Examples of haloalkylsulfanyl groups include groups of the formula -S- R', wherein R' is trifluoromethyl.

The term "haloalkylsulfonyl" denotes a group of the formula -S(0)2-R', wherein R' is a haloalkyl group. Examples of haloalkylsulfonyl groups include groups of the formula - S(0) ₂ -R', wherein R' is trifluoromethyl.

The term "halocycloalkyl" denotes a cycloalkyl group wherein at least one of the hydrogen atoms of the cycloalkyl group has been replaced by same or different halogen atoms, particularly fluoro atoms. Examples of halocycloalkyl include fluorocyclopropyl, difluorocyclopropyl, fluorocyclobutyl and difluorocyclobutyl.

The term "halogen" and "halo" are used interchangeably herein and denote fluoro, chloro, bromo, or iodo. Particular halogens are chloro and fluoro. Particular halogen is fluoro.

The term "heteroaryl" denotes a monovalent aromatic heterocyclic mono- or bicyclic ring system of 5 to 12 ring atoms, comprising 1, 2, 3 or 4 heteroatoms selected from N, O and S, the remaining ring atoms being carbon. Examples of heteroaryl include pyrrolyl, furanyl, thienyl, imidazolyl, oxazolyl, thiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridinyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, triazinyl, azepinyl, diazepinyl, isoxazolyl, benzofuranyl, isothiazolyl, benzothienyl, indolyl, isoindolyl, isobenzofuranyl, benzimidazolyl, benzoxazolyl, benzoisoxazolyl, benzothiazolyl, benzoisothiazolyl, benzooxadiazolyl, benzothiadiazolyl, benzotriazolyl, purinyl, quinolinyl, isoquinolinyl, quinazolinyl and quinoxalinyl. Particular heteroaryl are imidazolyl, pyridinyl and pyrazolyl.

The term "heteroarylalkyl" denotes an alkyl group wherein one of the hydrogen atoms of the alkyl group has been replaced an aryl group. Particular heteroarylalkyl group is heteroarylmethyl.

The term "pharmaceutically acceptable salts" refers to those salts which retain the biological effectiveness and properties of the free bases or free acids, which are not biologically or otherwise undesirable. The salts are formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, in particular hydrochloric acid, and organic acids such as acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, N-acetylcystein and the like. In addition these salts may be prepared by addition of an inorganic base or an organic base to the free acid. Salts derived from an inorganic base include, but are not limited to, the sodium, potassium, lithium, ammonium, calcium, magnesium salts and the like. Salts derived from organic bases include, but are not limited to salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, lysine, arginine, N- ethylpiperidine, piperidine, polyimine resins and the like. Particular pharmaceutically acceptable salts of compounds of formula (I) are the hydrochloride salts , methanesulfonic acid salts and citric acid salts.

"Pharmaceutically acceptable esters" means that compounds of general formula (I) may be derivatised at functional groups to provide derivatives which are capable of conversion back to the parent compounds in vivo. Examples of such compounds include physiologically acceptable and metabolically labile ester derivatives, such as

methoxymethyl esters, methylthiomethyl esters and pivaloyloxymethyl esters.

μAdditionally, any physiologically acceptable equivalents of the compounds of general formula (I), similar to the metabolically labile esters, which are capable of producing the parent compounds of general formula (I) in vivo, are within the scope of this invention. The term "protecting group" (PG) denotes the group which selectively blocks a reactive site in a multifunctional compound such that a chemical reaction can be carried out selectively at another unprotected reactive site in the meaning conventionally associated with it in synthetic chemistry. Protecting groups can be removed at the appropriate point. Exemplary protecting groups are amino-protecting groups, carboxy- protecting groups or hydroxy-protecting groups. Particular protecting groups are the tert- butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), fluorenylmethoxycarbonyl (Fmoc) and benzyl (Bn). Further particular protecting groups are the tert-butoxycarbonyl (Boc) and the fluorenylmethoxycarbonyl (Fmoc). More particular protecting group is the tert- butoxycarbonyl (Boc).

The abbreviation uM means microMolar and is equivalent to the symbol μΜ.

The compounds of the present invention can also contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute such compounds. For example, the present invention also embraces isotopically-labeled variants of the present invention which are identical to those recited herein, but for the fact that one or more atoms are replaced by an atom having the atomic mass or mass number different from the

predominant atomic mass or mass number usually found in nature for the atom. All isotopes of any particular atom or element as specified are contemplated within the scope of the compounds of the invention, and their uses. Exemplary isotopes that can be incorporated in to compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine, chlorine and iodine, such as 2 H ("D"), 3 H ("T"), ^U C, ¹³ C, ¹⁴ C, ¹³ N, ¹⁵ N, ¹⁵ 0, ¹⁷ 0, ¹⁸ 0, ³² P, ³³ P, ³⁵ S, ¹⁸ F, ³⁶ C1, ¹²³ I and ¹²⁵ I. Certain isotopically labeled compounds of the present invention (e.g., those labeled with ³ H or ¹⁴ C) are useful in compound and /or substrate tissue distribution assays. Tritiated ( H) and carbon- 14 ( ¹⁴ C) isotopes are useful for their ease of preparation and detectability. Further substitution with heavier isotopes such as deuterium (i.e., H) may afford certain therapeutic advantages resuting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some

circumstances. Positron emitting isotopes such as 15 13 11 18

O, N, C, and F are useful for positron emission tomography (PET) studies to examine substrate receptor occupancy. Isotopically labeled compounds of the present inventions can generally be prepared by following procedures analogous to those disclosed in the Schemes and/or in the Examples herein below, by substituting a non-isotopically labeled reagent with a isotopically labeled reagent. In particular, compounds of formula (I) wherein one or more H atom have been replaced by a H atom are also an embodiment of this invention. The compounds of formula (I) can contain several asymmetric centers and can be present in the form of optically pure enantiomers, mixtures of enantiomers such as, for example, racemates, optically pure diastereioisomers, mixtures of diastereoisomers, diastereoisomeric racemates or mixtures of diastereoisomeric racemates.

According to the Cahn-Ingold-Prelog Convention the asymmetric carbon atom can be of the "R" or "S " configuration.

Also an embodiment of the present invention are compounds according to formula (I) as described herein and pharmaceutically acceptable salts or esters thereof, in particular compounds according to formula (I) as described herein and pharmaceutically acceptable salts thereof, more particularly compounds according to formula (I) as described herein. Also a particular embodiment of the present invention are compounds according to formula (I) as described herein wherein R ¹ is alkyl.

Also a further particular embodiment of the present invention are compounds according to formula (I) as described herein wherein R ¹ is methyl.

A particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ⁵ , R ⁶ , R ⁷ and R ⁸ are H.

Another particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R is H.

Also an embodiment of the present invention are compounds according to formula (I) as described herein, wherein one of R ³ and R ⁴ is H and the other one is halogen.. A more particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ³ and R ⁴ are H. Another particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹⁶ is H or alkyl.

Also a particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R 12 , R 13 and R 17 are H. Another embodiment of the present invention are compounds according to formula

(I) as described herein, wherein R 12 , R 13 , R 16 and R IV are H.

Another embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹¹ and R ¹⁴ together form -CH ₂ -CH ₂ -, -CH ₂ (CH ₃ )-CH ₂ - or -CH ₂ -CH ₂ (CH ₃ )-. Another particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹¹ and R ¹⁴ together form -CH ₂ -CH ₂ .

Another embodiment of the present invention are compounds according to formula (I) as described herein, wherein A is -C(O)-.

A particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹⁰ is alkyl or heteroaryl substituted with one to three substituents selected from alkyl, halogen, cyano, alkoxy, alkylsulfanyl and alkylsulfonyl.

A further particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹⁰ is alkyl or substituted pyrazolyl, substituted imidazolyl or substituted pyridinyl, wherein substituted pyrazolyl, substituted imidazolyl and substituted pyridinyl are substituted with one to three substituents selected from alkyl, halogen, cyano, alkoxy, alkylsulfanyl and alkylsulfonyl.

A more particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹⁰ is alkyl or substituted pyrazolyl, substituted imidazolyl or substituted pyridinyl, wherein substituted pyrazolyl, substituted imidazolyl and substituted pyridinyl are substituted with one alkyl or one halogen. A further more particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹⁰ is substituted pyrazolyl, substituted imidazolyl or substituted pyridinyl, wherein substituted pyrazolyl, substituted imidazolyl and substituted pyridinyl are substituted with one alkyl or one halogen.

A even more particular embodiment of the present invention are compounds according to formula (I) as described herein, wherein R ¹⁰ is methylpyrazolyl,

methylimidazolyl or chloropyridinyl.

Particular examples of compounds of formula (I) as described herein are selected from

5-Fluoro-l-methyl-6-[4-[[4-(l-methylpyrazole-4-carbonyl)p iperazin-l-yl]methyl]- lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

5- Chloro- 1 -methyl-6- [4- [ [4- ( 1 -methylpyrazole-4-carbonyl)piperazin- 1 -yl] methyl] - lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

8-Chloro- 1 -methyl-6- [4- [ [4- ( 1 -methylpyrazole-4-carbonyl)piperazin- 1 -yl] methyl] - lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

6- [4-[[(3R)-4-Acetyl-3-methyl-piperazin-l-yl]methyl]-lH-pyrazo l-3-yl]-5-fluoro-l- methyl-3,4-dihydroquinolin-2-one;

5- Fluoro-l-methyl-6-[4-[[(3R)-3-methyl-4-(l-methylpyrazole-4-c arbonyl)piperazin- l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

6- [4-[[(3R)-4-Acetyl-3-methyl-piperazin-l-yl]methyl]-lH-pyrazo l-3-yl]-5-chloro-l- methyl-3,4-dihydroquinolin-2-one;

5- Chloro-l-methyl-6-[4-[[(3R)-3-methyl-4-(l-methylpyrazole-4-c arbonyl)piperazin- l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

6- [4-[[(3R)-4-Acetyl-3-methyl-piperazin-l-yl]methyl]-lH-pyrazo l-3-yl]-l-methyl- 3,4-dihydroquinolin-2-one; l-Methyl-6-[4-[[(3R)-3-methyl-4-(l-methylpyrazole-4-carbonyl )piperazin-l- yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

5-Chloro-l-methyl-6-[4-[[(2R)-2-methyl-4-(l-methylpyrazol e-4-carbonyl)piperazin- l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

5-Fluoro-l-methyl-6-[4-[[(2R)-2-methyl-4-(l-methylpyrazol e-4-carbonyl)piperazin- l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

5-Chloro-l-methyl-6-[4-[[2-(l-methylpyrazole-4-carbonyl)- 2,6- diazaspiro[3.3]heptan-6-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihy droquinolin-2-one;

5-Chloro-6-[4-[(2-ethylsulfonyl-2,6-diazaspiro[3.3]heptan -6-yl)methyl]-lH-pyrazol- 3 -yl] - 1 -methyl- 3 ,4-dihydroquinolin-2- one ;

5- Fluoro-l-methyl-6-[4-[[2-(l-methylpyrazole-4-carbonyl)-2,6- diazaspiro[3.3]heptan-6-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihy droquinolin-2-one;

6- [4-[(2-Ethylsulfonyl-2,6-diazaspiro[3.3]heptan-6-yl)methyl]- lH-pyrazol-3-yl]-l- methyl-3,4-dihydroquinolin-2-one;

6-[4-[(2-Isopropylsulfonyl-2,6-diazaspiro[3.3]heptan-6-yl )methyl]-lH-pyrazol-3-yl]- l-methyl-3,4-dihydroquinolin-2-one; l-Methyl-6-[4-[[2-(4-methylpyridine-3-carbonyl)-2,6-diazaspi ro[3.3]heptan-6- yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

6-[4-[(2-Acetyl-2,6-diazaspiro[3.3]heptan-6-yl)methyl]-lH -pyrazol-3-yl]-5-chloro- l-methyl-3,4-dihydroquinolin-2-one;

8-Chloro-l-methyl-6-[4-[[2-(l-methylpyrazole-4-carbonyl)- 2,6- diazaspiro[3.3]heptan-6-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihy droquinolin-2-one;

6-[4-[(2-Acetyl-2,6-diazaspiro[3.3]heptan-6-yl)methyl]-lH -pyrazol-3-yl]-8-chloro- l-methyl-3,4-dihydroquinolin-2-one;

8-Chloro-6-[4-[(2-ethylsulfonyl-2,6-diazaspiro[3.3]heptan -6-yl)methyl]-lH-pyrazol- 3 -yl] - 1 -methyl- 3 ,4-dihydroquinolin-2- one ; l-Methyl-6-[4-[[2-(l-methylimidazole-2-carbonyl)-2,6-diazasp iro[3.3]heptan-6- yl]methyl]- lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one;

6-[4-[[2-(3-Chloropyridine-2-carbonyl)-2,6-diazaspiro[3.3 ]heptan-6-yl]methyl]-lH- pyrazol-3-yl]-l-methyl-3,4-dihydroquinolin-2-one; and pharmaceutically acceptable salts thereof.

Processes for the manufacture of compounds of formula (I) as described herein are an object of the invention.

The preparation of compounds of formula (I) of the present invention may be carried out in sequential or convergent synthetic routes. Syntheses of the invention are shown in the following general schemes. The skills required for carrying out the reaction and purification of the resulting products are known to those persons skilled in the art. In case a mixture of enantiomers or diastereoisomers is produced during a reaction, these enantiomers or diastereoisomers can be separated by methods described herein or known to the persons skilled in the art such as e.g. chiral chromatography or crystallization. The substituents and indices used in the following description of the processes have the significance given herein.

The following abbreviations are used in the present text:

AcOH = acetic acid, BOC = t-butyloxycarbonyl, BuLi = butyllithium, CDI= 1, 1- carbonyldiimidazole, DCM = dichloromethane, DBU = 2,3,4,6,7,8,9, 10-octahydro- pyrimido[l,2-a]azepine, DCE = 1,2-dichloroethane, DIBALH = di- -butylaluminium hydride, DCC = N,N'-dicyclohexylcarbodiimide, DMA = N,N-dimethylacetamide, DMAP = 4-dimethylaminopyridine, DMF = N,N-dimethylformamide, EDCI = N-(3- dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, EtOAc = ethylacetate, EtOH = ethanol, Et ₂ 0 = diethylether, Et ₃ N = triethylamine, eq = equivalents, HATU = 0-(7- azabenzotriazol- l-yl)- l,l ,3,3-tetramethyluronium hexafluorophosphate, HPLC = high performance liquid chromatography, HOBT = 1-hydroxybenzo-triazole, Huenig's base = iPr ₂ NEt = iV-ethyl diisopropylamine, IPC= in process control, LAH = lithium aluminium hydride, LDA = lithium diisopropylamide, LiBH ₄ = lithium borohydride, MeOH = methanol, NaBH ₃ CN, sodium cyanoborohydride, NaBH ₄ = sodium borohydride, Nal = sodium iodide, Red-Al = sodium bis(2-methoxyethoxy) aluminium hydride, RT = room temperature, TBDMSC1 = t-butyldimethylsilyl chloride, TFA = trif uoroacetic acid, THF = tetrahydrofuran, quant = quantitative.

Prot. = Protecting Groip

X is Halogen or OS0 ₂ Alkyl/Aryl

R ¹⁰¹ and R ¹⁰² are both OH or, e.g. together with th

R ¹⁰³ stands for

Halo-pyrazole ester compounds 51 (Scheme 2) carrying a suitable protecting group, as e.g. methoxybenzyl, tetrahydropyranyl, (2-trimethylsilyl)ethoxymethoxy, can be reduced to the corresponding primary alcohols 52 (R ¹⁶ , R ¹⁷ =H), e.g. by using lithium

diisobutylaluminium hydride in a solvent like tetrahydrofuran in a temperature range between about -78 °C and room temperature. Alternatively, halo-pyrazole ester compounds 51 can be transformed into secondary or tertiary alcohols 52 by the following reaction sequence: i) saponification; ii) transformation into methoxy-N-methyl-amides (Weinreb amides); iii) conversion into ketones by reaction with Grignard reagents R ¹⁶ MgX or lithium reagents R ¹⁶ Li in solvents like THF in a temperature range between -78 °C and room temperature; iv) reduction with lithium diisobutylaluminium hydride or with sodium borohydride or reaction with Grignard reagents R 17 MgX or lithium reagents R 17 Li in solvents like THF in a temperature range between -78 °C and room temperature (step a).

Haloalkyl compounds 53 can be obtained from hydroxy-alkyl compounds 52 by transformation of the OH into e.g. a chloro or bromo function e.g. by treatment with thionyl chloride or phosphorus tribromide in a solvent like DCM around room

temperature, by reaction with methanesulfonyl chloride, Et ₃ N or 2,4,6-trimethylpyridine, DCM or DMF, and optionally LiCl or by reaction with triphenylphosphine/CCl ₄ in CH ₃ CN, both procedures performed preferably between 0 °C and room temperature (step b). Halo-alkyl derivatives 53 react with primary or secondary amine compounds in solvents like dichloromethane and in the presence of a base as e.g. diisopropyl ethylamine preferably around room temperature to give amine linked halo-pyrazole compounds 60 (step f). Alternatively, halo-pyrazole compounds 60 can be formed from hydroxy compounds 52 by oxidation of the OH-function, e.g. using Swern conditions followed by reductive amination with amino compounds 59, e.g. by reacting with NaBH(OAc) ₃ and Et ₃ N in CHC1 ₃ around room temperature (steps g, h). Condensation of amine linked halo- pyrazole compounds 60 with boronic acid or ester compounds 56 (known in the art or being prepared as described in Schemes 5 and 6) can be performed using Suzuki conditions, e.g. in the presence of catalysts, such as tri-o- tolylphosphine/palladium(II)acetate, ieirafc 5 ^, -(triphenylphosphine)-palladium,

b 5 ^, (triphenylphosphine)palladium(II)chloride or dichloro[ 1 , 1 '-bzs'(diphenylphosphino)- ferrocene]palladium(II) optionally in the form of a dichloromethane complex (1: 1), and in the presence of a base, such as aqueous or non aqueous potassium phosphate, sodium or potassium carbonate, in a solvent, such as dimethylsulfoxide, toluene, ethanol, dioxane, tetrahydrofuran or N,N-dimethylformamide, and in an inert atmosphere such as argon or nitrogen, in a temperature range preferably between room temperature and about 130 °C leading to pyrazoles 61 (steps d). Removal of the protecting group in pyrazoles 61 then leads to pyrazoles 62 (e.g. treatment with trifluoroacetic acid under microwave conditions at temperatures around 100 °C can be used for removal of a p-methoxy-benzyl protecting group, treatment with 4M HCl in dioxane in MeOH around room temperature can be used for removal of a THP protecting group) (step e).

Scheme 5

Chloropropionic acid anilides 201 (Scheme 5) can be prepared from chloro propionic acid chlorides and anilines 200 by reaction in a solvent like DCM in the presence of a base like pyridine preferably around room temperature (step a). Chloropropionic acid anilides

201 undergo ring closure to lactam compounds 202 when treated with A1C1 ₃ preferably without solvent at elevated temperatures of e.g. 100 to 150 °C (step b). Lactam compounds

202 can be alkylated at nitrogen using a base like sodium hydride or sodium or potassium iert-butoxide, followed by addition of an alkylating agent of formula R ^x -Y, wherein Y is halogen, tosylate or mesylate, in a solvent like DMF or THF preferably in a temperature range between 0 °C and about 80 °C giving N-alkylated lactams 203 (step c). Optional halogenation of N- alkylated lactams 203 can be performed e.g. by using N-bromo or N- chloro succinimide in solvents like DMF preferably around room temperature giving halo lactam compounds 204 with X equal to bromine or chlorine respectively (step d). Reaction of lactams 204 with e.g. 4,4,4',4',5,5,5',5'-octamethyl-2,2'-b (l,3,2-dioxaborolane) in solvents like dimethylsulfoxide or dioxane in the presence of potassium acetate and catalysts like (l, r-b 5 ^, -diphenylphosphino)-ferrocene)palladium-(II)dichloride (1: 1 complex with dichloromethane) at temperatures up to about 100 °C gives boronic ester compounds 56 (step e).

Scheme 6

Treatment of nitro-benzene derivatives 250 e.g. with N-bromo succinimide, benzoyl peroxide (BPO) in a solvent like CC1 ₄ preferably at reflux gives bromo-methyl compounds 251 (Scheme 6, step a). Reaction of bromo-methyl compounds 251 and a suitable malonic ester derivative, preferable in the presence of cesium carbonate in a solvent like DMF between 0 °C and room temperature gives malonic ester adducts 252 (step b). Reduction of the nitro malonic ester derivatives 252 with e.g. stannous chloride dihydrate in 6 M aq. HC1 at elevated temperature (e.g. 130 °C) directly leads to lactam compounds 253 (step c). Lactam compounds 253 can be alkylated at nitrogen, halogenated and converted into boronic ester compounds 256 as described for the preparation of compounds 56 (Scheme 5) (steps d, e, f).

Also an embodiment of the present invention is a process to prepare a compound of formula (I) as defined above comprising the reaction of a compound of formula (II) in the presence of a compound of formula (III);

(ii) wherein R ¹ , R ² , R ³ , R ¹⁴ , R ¹⁵ , R ¹⁶ , R ¹⁷ and m are described herein and X is halogen or hydroxy.

In particular, in the presence of a base, in particular triethylamine, in a solvent, in particular in dichloromethane, optionally in the presence of a coupling agent, in particular HATU, and at temperature between about -20 °C and the room temperature. Also an object of the present invention is a compound according to formula (I) as described herein for use as therapeutically active substance.

Likewise an object of the present invention is a pharmaceutical composition comprising a compound according to formula (I) as described herein and a therapeutically inert carrier.

The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom.

The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of diabetic nephropathy.

The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of kidney or heart fibrosis.

The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of chronic kidney disease. The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of congestive heart failure.

The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of hypertension.

The present invention also relates to the use of a compound according to formula (I) as described herein for the treatment or prophylaxis of primary aldosteronism.

A particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom.

Also a particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of diabetic nephropathy. Another particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of kidney or heart fibrosis.

Also a particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of chronic kidney disease. Also a particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of congestive heart failure.

Also a particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of hypertension.

Also a particular embodiment of the present invention is a compound according to formula (I) as described herein for the treatment or prophylaxis of primary aldosteronism.

The present invention also relates to the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom. The present invention also relates to the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of diabetic nephropathy.

The present invention also relates to the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of kidney or heart fibrosis.

Also an embodiment of the present invention is the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of chronic kidney disease.

Also an embodiment of the present invention is the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of congestive heart failure. Also an embodiment of the present invention is the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of hypertension.

Also an embodiment of the present invention is the use of a compound according to formula (I) as described herein for the preparation of a medicament for the treatment or prophylaxis of primary aldosteronism.

Also an object of the invention is a method for the treatment or prophylaxis of chronic kidney disease, congestive heart failure, hypertension, primary aldosteronism and Cushing syndrom, which method comprises administering an effective amount of a compound according to formula (I) as described herein.

Also an object of the invention is a method for the treatment or prophylaxis of diabetic nephropathy, which method comprises administering an effective amount of a compound according to formula (I) as described herein.

Also an object of the invention is a method for the treatment or prophylaxis of kidney or heart fibrosis, which method comprises administering an effective amount of a compound according to formula (I) as described herein.

Also an embodiment of the present invention is a method for the treatment or prophylaxis of chronic kidney disease, which method comprises administering an effective amount of a compound according to formula (I) as described herein. Also an embodiment of the present invention is a method for the treatment or prophylaxis of congestive heart failure, which method comprises administering an effective amount of a compound according to formula (I) as described herein.

Also an embodiment of the present invention is a method for the treatment or prophylaxis of hypertension, which method comprises administering an effective amount of a compound according to formula (I) as described herein.

Also an embodiment of the present invention is a method for the treatment or prophylaxis of primary aldosteronism, which method comprises administering an effective amount of a compound according to formula (I) as described herein. Also an embodiment of the present invention is a compound of formula (I) as described herein, when manufactured according to any one of the described processes.

Assay procedures

Herein we identified the use of the G-402 cell line as a host cell to ectopically express (transiently or stably) enzymes of the CYPl 1 family. Specifically we developed stable G-402 cells expressing ectopically human CYPl 1B1, human CYPl 1B2, human CYP11A1, cynmolgus CYP11B1 or cynomolgus CYP11B2 enzyme activity. Importantly the identified cell line G-402 expresses co-factors (adrenodoxin and adrenodoxin reductase) important for the activity of the CYPl 1 family and no relevant enzyme activity of the CYPl 1 family (in comparison to H295R cells) was detected in these cells. Therefore the G-402 cell line is uniquely suited as a host cell for the ectopic expression of enzymes from the CYP11 family.

G-402 cells can be obtained from ATCC (CRL-1440) and were originally derived from a renal leiomyoblastoma. The expression plasmids contains the ORF for either human / cyno CYPl IB 1 or

CYPl 1B2 under the control of a suitable promoter (CMV-promoter) and a suitable resistance marker (neomycin). Using standard techniques the expression plasmid is transfected into G-402 cells and these cells are then selected for expressing the given resistance markers. Individual cell-clones are then selected and assessed for displaying the desired enzymatic activity using 11 -Deoxycorticosterone (Cypl 1B2) or 11-Deoxycortisol (Cypl lBl) as a substrate.

G-402 cells expressing CYP11 constructs were established as described above and maintained in McCoy's 5a Medium Modified, ATCC Catalog No. 30-2007 containing 10% FCS and 400 μg/ml G418 (Geneticin) at 37 °C under an atmosphere of 5% C02/95% air. Cellular enzyme assays were performed in DMEM/F12 medium containing 2.5 % charcoal treated FCS and appropriate concentration of substrate (0.3-10 uM 11- Deoxycorticosterone, 11-Deoxycortisol or Corticosterone). For assaying enzymatic activity, cells were plated onto 96 well plates and incubated for 16h. An aliquot of the supernatant is then transferred and analyzed for the concentration of the expected product (Aldosterone for CYPl 1B2; Cortisol for CYPl IB 1). The concentrations of these steroids can be determined using HTRF assays from CisBio analyzing either Aldosterone or Cortisol.

Inhibition of the release of produced steroids can be used as a measure of the respective enzyme inhibition by test compounds added during the cellular enzyme assay. The dose dependent inhibition of enzymatic activity by a compound is calculated by means of plotting added inhibitor concentrations (x-axes) vs. measured steroid/product level (y-axes). The inhibition is then calculated by fitting the following 4-parameter sigmoidal function (Morgan-Mercer-Flodin (MMF) model) to the raw data points using the least squares method:

ΛΒ + Cx ¹

y wherein, A is the maximum y value, B is the EC50 factor determined using XLFit, C is the minimum y value and D is the slope value.

The maximum value A corresponds to the amount of steroid produced in the absence of an inhibitor, the value C corresponds to the amount of steroid detected when the enzyme is fully inhibited.

EC50 values for compounds claimed herein were tested with the G402-based assay system described. Cypl 1B2 enzyme activity was tested in presence of 1 μΜ

Deoxycorticosterone and variable amounts of inhibitors; Cypl lBl enzyme activity was tested in presence of 1 μΜ Deoxycortisol and variable amounts of inhibitors.

EC50 EC50 EC50 EC50 human human human human

Example CYP11B2 CYP11B1 Example CYP11B2 CYP11B1 nM nM nM nM

42 0.0341 17.81 43 0.0311 33.7504 EC50 EC50 EC50 EC50 human human human human

Example CYP11B2 CYP11B1 Example CYP11B2 CYP11B1 nM nM nM nM

44 0.3959 > 30 55 1.772 9.2895

45 0.1948 6.0498 56 0.7683 > 30

46 0.0113 2.5722 57 0.9634 9.0499

47 0.1528 7.2822 58 8.4993 19.8471

48 0.0083 3.1958 59 0.6953 > 30

49 0.4815 > 30 60 0.8914 > 30

50 0.0108 6.115 61 0.2589 23.9287

51 0.2398 32.0482 62 0.1081 > 30

52 0.1473 20.5075 63 7.2808 > 30

53 9.4806 > 30 64 4.6406 > 30

54 1.4396 > 30

Compounds of formula (I) and their pharmaceutically acceptable salts or esters thereof as described herein have EC ₅₀ (CYP11B2) values between 0.000001 uM and 1000 uM, particular compounds have EC ₅ o (CYP11B2) values between 0.00005 uM and 500 uM, further particular compounds have EC ₅₀ (CYP11B2) values between 0.0005 uM and 50 uM, more particular compounds have EC ₅₀ (CYP11B2) values between 0.0005 uM and 5 uM. These results have been obtained by using the described enzymatic assay.

The compounds of formula (I) and their pharmaceutically acceptable salts can be used as medicaments (e.g. in the form of pharmaceutical preparations). The

pharmaceutical preparations can be administered internally, such as orally (e.g. in the form of tablets, coated tablets, dragees, hard and soft gelatin capsules, solutions, emulsions or suspensions), nasally (e.g. in the form of nasal sprays) or rectally (e.g. in the form of suppositories). However, the administration can also be effected parentally, such as intramuscularly or intravenously (e.g. in the form of injection solutions). The compounds of formula (I) and their pharmaceutically acceptable salts can be processed with pharmaceutically inert, inorganic or organic adjuvants for the production of tablets, coated tablets, dragees and hard gelatin capsules. Lactose, corn starch or derivatives thereof, talc, stearic acid or its salts etc. can be used, for example, as such adjuvants for tablets, dragees and hard gelatin capsules. Suitable adjuvants for soft gelatin capsules, are, for example, vegetable oils, waxes, fats, semi-solid substances and liquid polyols, etc.

Suitable adjuvants for the production of solutions and syrups are, for example, water, polyols, saccharose, invert sugar, glucose, etc.

Suitable adjuvants for injection solutions are, for example, water, alcohols, polyols, glycerol, vegetable oils, etc.

Suitable adjuvants for suppositories are, for example, natural or hardened oils, waxes, fats, semi-solid or liquid polyols, etc.

Moreover, the pharmaceutical preparations can contain preservatives, solubilizers, viscosity-increasing substances, stabilizers, wetting agents, emulsifiers, sweeteners, colorants, flavorants, salts for varying the osmotic pressure, buffers, masking agents or antioxidants. They can also contain still other therapeutically valuable substances.

The dosage can vary in wide limits and will, of course, be fitted to the individual requirements in each particular case. In general, in the case of oral administration a daily dosage of about 0.1 mg to 20 mg per kg body weight, preferably about 0.5 mg to 4 mg per kg body weight (e.g. about 300 mg per person), divided into preferably 1-3 individual doses, which can consist, for example, of the same amounts, should be appropriate. It will, however, be clear that the upper limit given herein can be exceeded when this is shown to be indicated. In accordance with the invention, the compounds of formula (I) or their

pharmaceutically acceptable salts and esters can be used for the treatment or prophylaxis of aldosterone mediated diseases.

The compounds of formula (I) or their pharmaceutically acceptable salts and esters herein are inhibitiors of CYP11B2. The compounds of formula (I) or their

pharmaceutically acceptable salts and esters herein display also variable inhibition of CYPl lBl but present an improved selectivity towards CYP11B2 versus CYPl lBl.. Such compounds may be used for treatment or prophylaxis of conditions displaying excessive Cortisol production/levels or both excessive Cortisol and aldosterone levels (for ex.

Cushing syndrome, burn trauma patients, depression, post-traumatic stress disorders, chronic stress, corticotrophic adenomas, Morbus Cushing).

In accordance with the invention, the compounds of formula (I) or their pharmaceutically acceptable salts and esters can be used for the treatment or prophylaxis of cardiovascular conditions (including hypertension and heart failure), vascular conditions, endothelial dysfunction, baroreceptor dysfunction, renal conditions, liver conditions, fibrotic diseases, inflammatory conditions, retinopathy, neuropathy (such as peripheral neuropathy), pain, insulinopathy, edema, edematous conditions, depression and the like.

Cardiovascular conditions include congestive heart failure, coronary heart disease, arrhythmia, arterial fibrillation, cardiac lesions, decreased ejection fraction, diastolic and systolic heart dysfunction, fibrinoid necrosis of coronary arteries, cardiac fibrosis, hypertrophic cardiomyopathy, impaired arterial compliance, impaired diastolic filling, ischemia, left ventricular hypertrophy, myocardial and vascular fibrosis, myocardial infarction, myocardial necrotic lesions, cardiac arrhythmias, prevention of sudden cardiac death, restenosis, stroke, vascular damage.

Renal conditions include acute and chronic renal failure, nephropathy, end- stage renal disease, diabetic nephropathy, decreased creatinine clearance, decreased glomerular filtration rate, expansion of reticulated mesangial matrix with or without significant hypercellularity, focal thrombosis of glomerular capillaries, global fibrinoid necrosis, glomerulosclerosis, ischemic lesions, malignant nephrosclerosis (such as ischemic retraction, microalbuminuria, proteinuria, reduced renal blood flow, renal arteriopathy, swelling and proliferation of intracapillary (endothelial and mesangial) and/or

extracapillary cells (crescents).

Renal conditions also include glomerulonephritis (such as diffuse proliferative, focal proliferative, mesangial proliferative, membranoproliferative, minimal change

membranous glomerulonephritis), lupus nephritis, non-immune basement membrane abnormalities (such as Alport syndrome), renal fibrosis and glomerulosclerosis (such as nodular or global and focal segmental glomerulosclerosis).

Liver conditions include, but are not limited to, liver steatosis, nonalcoholic steatohepatitis, liver cirrhosis, liver ascites, hepatic congestionand the like.

Vascular conditions include, but are not limited to, thrombotic vascular disease (such as mural fibrinoid necrosis, extravasation and fragmentation of red blood cells, and luminal and/or mural thrombosis), proliferative arteriopathy (such as swollen myointimal cells surrounded by mucinous extracellular matrix and nodular thickening),

atherosclerosis, decreased vascular compliance (such as stiffness, reduced ventricular compliance and reduced vascular compliance), endothelial dysfunction, and the like.

Inflammatory conditions include, but are not limited to, arthritis (for example, osteoarthritis), inflammatory airways diseases (for example, chronic obstructive pulmonary disease (COPD)), and the like. Pain includes, but is not limited to, acute pain, chronic pain (for example, arthralgia), and the like.

Edema includes, but is not limited to, peripheral tissue edema, hepatic congestion, liver ascites, splenic congestion, respiratory or lung congestion, and the like.

Insulinopathies include, but are not limited to, insulin resistance, Type I diabetes mellitus, Type II diabetes mellitus, glucose sensitivity, pre-diabetic state, pre-diabetes, syndrome X, and the like.

Fibrotic diseases include, but are not limited to myocardial and intrarenal fibrosis, renal interstitial fibrosis and liver fibrosis. Furthermore, the compounds of formula (I) or their pharmaceutically acceptable salts and esters as described herein can also be used for the treatment or prophylaxis of cardiovascular condition selected from the group consisting of hypertension, heart failure (particularly heart failure post myocardial infarction), left ventricular hypertrophy, and stroke.

In another embodiment, the cardiovascular condition is hypertension.

In particular embodiment, the cardiovascular condition is treatment-resistant hypertension.

In another embodiment, the cardiovascular condition is heart failure. In another embodiment, the cardiovascular condition is left ventricular hypertrophy.

In another embodiment, the cardiovascular condition is congestive heart failure, more particularly in patients with preserved left ventricular ejection fraction.

In another embodiment, the cardiovascular condition is stroke.

In another embodiment, the compounds of formula (I) or their pharmaceutically acceptable salts and esters can be used for the treatment or prophylaxis renal condition.

In another embodiment, the renal condition is nephropathy.

In another embodiment, the renal condition is auto-immune glomerulonephritis.

In another embodiment, the chronic kidney disease is diabetic nephropathy.

In another embodiment, the fibrotic disease is kidney or heart fibrosis. In another embodiment, the compounds of formula (I) or their pharmaceutically acceptable salts and esters can be used for the treatment or prophylaxis Type II diabetes mellitus.

In another embodiment, the compounds of formula (I) or their pharmaceutically acceptable salts and esters can be used for the treatment or prophylaxis Type I diabetes mellitus. In another embodiment, the compounds of formula (I) or their pharmaceutically acceptable salts and esters can be used for the treatment or prophylaxis of diabetic retinopathy.

The invention is illustrated hereinafter by Examples, which have no limiting character.

In case the preparative examples are obtained as a mixture of enantiomers, the pure enantiomers can be separated by methods described herein or by methods known to the persons skilled in the art, such as e.g. chiral chromatography or crystallization.

Examples

All examples and intermediates were prepared under argon atmosphere if not specified otherwise.

Pyrazoles carrying a hydrogen substituent at any of the two nitrogen atoms and not symmetrical substituents at the 3 carbon atoms always exist in two tautomeric forms. Formulas and names describe any of the two forms.

Intermediate A-3

l-Methyl-6-(4,4,5,5 etramethyl-l,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-on e

ΓΑ1 6-Bromo-l-methyl-3,4-dihvdroquinolin-2-one

To a solution of 6-bromo-3,4-dihydro-lH-quinolin-2-one (5 g, 22.1 mmol) in DMF (100 mL) cooled to 0 °C was added potassium ie/t-butoxide (4.96 g, 44.2 mmol) portion wise and the reaction mixture was stirred at 0 °C for 15 min. Then, methyl iodide (4.08 g, 28.8 mmol) was added and the reaction mixture allowed to warm up to room temperature and stirring was continued overnight. More methyl iodide (1.25 g, 8.86 mmol) was added and the reaction mixture was heated to 40 °C until completion of the reaction. The mixture was diluted with EtOAc, poured into 100 mL of IN HCl and the aqueous phase was extracted with EtOAc (2 x 200 mL). The combined organics were washed with brine, dried over anhy. Na ₂ S0 ₄ , filtered and evaporated to dryness. The residue was purified by silica gel flash chromatography eluting with a 0 to 30% EtO Ac-heptane gradient to give the title compound (4.23 g, 80 %) as an off white solid. MS: 240.0, 242.1 (M+H ⁺ ).

ΓΒ1 l-Methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-3,4 -dihvdroquinolin-2-one

A flask was charged with 6-bromo-l-methyl-3,4-dihydroquinolin-2-one (3 g, 12.5 mmol), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-b (l,3,2-dioxaborolane) (3.81 g, 15.0 mmol), potassium acetate (3.68 g, 37.5 mmol) and dioxane (48 mL). The mixture was purged with Ar, then dichloro[l, -b 5'(diphenylphosphino)-ferrocene]palladium(II) dichloromethane complex (1: 1) [PdCl ₂ (DPPF) dichloromethane adduct] (457 mg, 0.560 mmol) was added and the resulting mixture was heated to 80 °C overnight. The reaction mixture was diluted with EtOAc, filtered through Celite and washed with EtOAc (2 x 150 mL). The resulting filtrate was washed with brine, dried over anhy. Na ₂ S0 ₄ , filtered and evaporated to dryness. The residue was purified by silica gel flash chromatography eluting with a 0 to 40% EtO Ac- heptane gradient to give the title compound (2.63 g, 73 %) as an off white solid. MS: 288.0 (M+H ⁺ ).

Intermediate A-4

7-Fluoro-l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborol an-2-yl)-3,4- dihydroquinolin-2-one

ΓΑ1 3-Chloro-N-(3-fluoro-phenyl)-propionamide

To a solution of 3-fluoroanilline (10 mL, 104.02 mmol) in DCM (100 mL) was added pyridine (21 mL, 260.2 mmol) and 3-chloropropionyl chloride (12 mL, 124.4 mmol). The reaction mixture was stirred for 3 h at room temperature until all starting materials had disappeared as shown by LC-MS analysis. The reaction mixture was then diluted with H ₂ 0 and extracted with EtOAc. The organic layer was dried over anhy. Na ₂ S0 ₄ and

concentrated in vacuo to afford the title compound as a solid. It was used in the next step without further purification. ΓΒ 1 7 -Fluoro- 3 ,4-dih vdro - 1 H-quinolin-2- one

A flame-dried 50-mL flask equipped with a magnetic stirring bar was charged with 3- chloro-N-(3-fluoro-phenyl)-propionamide (10 g, 49.6 mmol) and A1C1 ₃ (23.1 g, 173.6 mmol). On a pre-heated oil bath, the flask was heated to 120-125 °C for 2 h until LC-MS indicated the reaction was complete. After cooling to room temperature, the mixture was treated slowly with ice- water. After extraction with EtOAc, combined organics were washed with water and brine in sequence. The organic layer was dried over anhy. Na ₂ S0 ₄ , filtered and concentrated in vacuo to afford a white solid (7.63 g, 93.2%). rCl 7-Fluoro-l-methyl-3,4-dihydro-lH-quinolin-2-one

F

To an ice cold solution of 7-fluoro-3,4-dihydro-lH-quinolin-2-one (16.5 g, 0.1 mol) in DMF (200 mL) was added potassium ie/t-butoxide (22.4 g, 0.2 mol) in 2 portions. The reaction mixture was stirred at 0 °C for 30 min before methyl iodide (25.4 g, 0.18 mol) was added. After the addition, the reaction mixture was allowed to warm up to room temperature slowly and stirred at room temperature overnight. It was then diluted with EtOAc (500 mL) and poured into 200 mL of 1 N aq. HCl. After extraction with EtOAc (3 x 200 mL), the combined organics were washed with brine, dried over anhy. Na ₂ S0 ₄ , filtered and concentrated in vacuo to give the crude title compound as oil (16.0 g, 89 %). It was used in the next step without further purification. ΓΡ1 6-Bromo-7-fluoro-l-methyl-3,4-dihydro-lH-quinolin-2-one

A mixture of 7-fluoro-l-methyl-3,4-dihydroquinolin-2-one (2.56 g, 0.014 mol) and NBS (3.0 g, 0.017 mol) in DMF (30 mL) was stirred for 12 h at 25 °C. The reaction solution was diluted with H ₂ 0 (80 mL), and extracted with EtOAc (3 x 100 mL). The combined organics were washed by brine (3 x 100 mL), dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give the title compound (1.5 g, 42%) as white foam. MS: 258.0, 259.9 (M+H ⁺ ).

ΓΕ1 7-Fluoro-l-methyl-6-(4,4,5,5-tetramethyl-ri,3,21dioxaborolan -2-yl)-3,4-dihydro-lH- quinolin-2-one

In analogy to the procedure described for the preparation of intermediates A-3[B], 6- bromo-7-fluoro-l-methyl-3,4-dihydro-lH-quinolin-2-one was reacted with 4,4,4',4',5,5,5',5'-octamethyl-2,2'-b (l,3,2-dioxaborolane) in presence of potassium acetate and PdCl ₂ (DPPF)-CH ₂ Cl ₂ to give the title compound as a white solid. MS: 306.1 (M+H ⁺ ).

Intermediate A-5

8-Chloro-l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborol an-2-yl)-3,4- dihydroquinolin-2-one

ΓΑ1 N-(4-Bromo-2-chloro-phenyl)-3-chloro-propionamide

To a solution of 4-bromo-2-chloro-phenylamine (32 g, 0.15 mol) and pyridine (13.45 g, 0.17 mol) in DCM (200 mL) was added 3-chloropropionyl chloride (21.65 g, 0.17 mol) dropwise at 15 °C. After stirring at room temperature for 1 hour, the mixture was washed with water and then aqueous 2N HC1. The organic layer was dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to afford title compound (10.9 g, 90%) as a white solid.

ΓΒ1 6-Bromo-8-chloro-3,4-dihydro- lH-quinolin-2-one

A flame-dried 500-mL flask equipped with a magnetic stirring bar was charged with N-(4- bromo-2-chloro-phenyl)-3-chloro-propionamide (29.7 g, 0.1 mol) and aluminium chloride (53.3 g, 0.4 mol). In a pre-heated oil bath, the flask was heated to 140 °C for 1 h. After cooling to room temperature, the mixture was treated slowly with ice-water and extracted with EtOAc. The organic layer was washed with water and brine in sequence, dried over anhy. Na ₂ S0 _4i filtered, and concentrated in vacuo. The resulting residue was then purified by silica gel flash chromatography (30% ethyl acetate in hexane) to afford the title compound (7.0 g, 27%) as a white solid.

TCI 6-Bromo-8-chloro-l-methyl-3,4-dihvdro-lH-quinolin-2-one

A solution of 6-bromo-8-chloro-3,4-dihydro-lH-quinolin-2-one (7.0 g, 26.9 mmol) in DMF (100 mL) was treated with potassium iert-butoxide (6.0 g, 53.8 mmol) at 0 °C portion wise. The resulting mixture was stirred at 0 °C for 30 min before methyl iodide (5.0 g, 35.0 mmol) was added. After stirring for 12 h, the reaction mixture was treated with water, extracted with EtOAc. The organic layer was washed with water and brine in sequence, and dried over anhy. Na ₂ S0 ₄ . After removal of solvent under reduced pressure, the crude product (3.3 g, 45%) was obtained as a white solid.

ΓΡ1 8-Chloro-l-methyl-6-(4,4,5,5-tetramethyl-ri,3,21dioxaborolan -2-yl)-3,4-dihydro-lH- quinolin-2-one

A mixture of 6-bromo-8-chloro-l-methyl-3,4-dihydro-lH-quinolin-2-one (0.23 g, 0.84 mmol), b 5 ^, (pinacolato)diboron (0.255 g, l.Olmmol), l,l'-b 5 ^, (diphenylphosphino)- ferrocene-dichloropalladium (II), dichloromethane complex (1: 1) (30.7 mg, 0.04 mmol) and potassium acetate (0.247 g, 2.52 mmol) in dioxane (5 mL) was heated in a microwave at 80 °C overnight. After dilution with EtOAc, the organic layer was washed with water, dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo. The residue was then purified by silica gel flash chromatography (30% ethyl acetate in hexane) to afford the title compound (0.17g, 63%) as a white solid. MS: 322.2 (M+H ⁺ ).

Intermediate A-6

5-Fluoro-l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborol an-2-yl)-3,4- dihydroquinolin-2-one

Γ Al 2- (Bromomethyl) - 1 -f uoro- 3 -nitro-benzene

To a stirred solution of l-fluoro-2-methyl-3 -nitro-benzene (100 g, 0.64 mol) and benzoyl peroxide (BPO) (15 g, 64 mmol) in CC1 ₄ (1500 mL) was added NBS (127 g, 0.73 mmol). The resulting reaction mixture was heated to reflux for 12 h at 80 °C. After TLC (PE: EA = 20: 1) indicated the completion of reaction, the reaction mixture was concentrated to remove CC1 ₄ . The residue was diluted with DCM (500 mL), and washed with brine (2 x 300 mL). The organic layer was dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give a crude title product (160 g, 80%). It was used in the next step without further purification.

ΓΒ1 Diethyl 2-r(2-f uoro-6-nitro-phenyl)methyllpropanedioate

To a stirred solution of NaH (26 g, 0.65 mol) in DMF (500 mL) was added diethyl malonate (106 g, 0.66 mol) in 200 mL of DMF at 0 °C. It was stirred for 30 min at 0 °C before adding a solution of 2-(bromomethyl)-l-fluoro-3-nitro-benzene (128 g, 0.55 mol) in DMF (600 mL). The mixture was then allowed to warm up to room temperature and stirring was continued for 3 h before quenching by the addition of satd. aq. NH ₄ C1 (500 mL). The mixture was diluted with H ₂ 0 (2 L), and the aqueous layer was extracted with EtOAc (3 x 800 mL). The combined organics were washed with brine (3 x 500 mL), dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give a crude title product (180 g, 70%). It was used in the next step without further purification.

TCI Ethyl 5-fluoro-2-oxo-3,4-dihvdro-lH-quinoline-3-carboxylate

To a stirred solution of diethyl 2-[(2-fluoro-6-nitro-phenyl)methyl]propanedioate (126 g, 0.4 mol) and NH ₄ C1 (103 g, 2.4 mol) in EtOH /H ₂ 0 (5: 1, 1200 mL) was added iron powder (67 g, 1.2 mol) at 80 °C portion wise. The resulting mixture was refluxed for 2 h at 80 °C. After cooling to room temperature, the reaction mixture was filtered and the filtrate was concentrated in vacuo to give a crude title product (92 g, 50%). MS: 238.1 (M+H ⁺ ). It was used in the next step without further purification.

ΓΡ1 5-Fluoro-3,4-dihydro-lH-quinolin-2-one

A solution of ethyl 5-fluoro-2-oxo-3,4-dihydro-lH-quinoline-3-carboxylate (46 g, 0.19 mol) in AcOH (300 mL) and HC1 (150 mL) was heated to 90 °C for 1 h before it was concentrated under reduced pressure. Saturated aqueous Na ₂ C0 ₃ solution (500 mL) was carefully added to the residue, then it was diluted with additional H ₂ 0 (1 L), and extracted with EtOAc (3 x 300 mL). The combined organics were washed with brine (2 x 500 mL), dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give a crude title product (28 g, 89.3%). MS: 166.1 (M+H ⁺ ). It was used in the next step without further purification.

ΓΕ1 5-Fluoro-l-methyl-3,4-dihydroquinolin-2-one

To a solution of NaH (8.1 g, 0.20 mol) in DMF (200 mL) was added 5-fluoro-3,4-dihydro- lH-quinolin-2-one (28 g, 0.17 mol) in 100 mL of DMF at 0 °C. The resulting reaction mixture was stirred at 0 °C for 10 min before methyl iodide (30 g, 0.21 mmol) was added. After the addition, it was allowed to warm up to room temperature and stirred for 1 h. The reaction was then quenched by the addition of satd. aq. NH ₄ C1 (200 mL), diluted with H ₂ 0 (1 L), and extracted with EtOAc (3 x 300 mL). The combined organics were washed with brine (3 x 500 mL), dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give crude title compound (32 g, 80%). MS: 180.1 (M+H ⁺ ). It was used in the next step without further purification. rFl 6-Bromo-5-fluoro-l-methyl-3,4-dihydroquinolin-2-one

To a solution of 5-fluoro-l-methyl-3,4-dihydroquinolin-2-one (25.6 g, 0.14 mol) in DMF (300 mL) was added NBS (30 g, 0.17 mol) and the resulting reaction mixture was stirred at room temperature for 12 h. It was diluted with H ₂ 0 (800 mL), and the aqueous layer was extracted with EtOAc (3 x 200 mL). The combined organics were washed with brine (3 x 300 mL), dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give crude title compound (15 g, 42%). MS: 256.1 and 258.1 (M+H ⁺ ).

TGI 5-Fluoro-l-methyl-6-(4,4,5,5-tetramethyl-ri,3,21dioxaborolan -2-yl)-3,4-dihydro-lH- quinolin-2-one

A flask was charged with 6-bromo-5-fluoro-l-methyl-3,4-dihydroquinolin-2-one (14.5 g, 56 mmol), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-b (l,3,2-dioxaborolane) (28 g, 113 mmol), potassium acetate (11 g, 113 mmol) and DMSO (300 mL). The mixture was purged with Ar, then dichloro[l,l'-b 5'(diphenylphosphino)-ferrocene]palladium(II) dichloromethane complex (1: 1) [PdCl ₂ (DPPF)-CH ₂ Cl ₂ adduct] (2.2 g, 2.8 mmol) was added and the resulting mixture was heated to 80 °C for 3 h. The reaction mixture was filtered and the filtrate was diluted with satd. aq. NH ₄ C1 (200 mL) and H ₂ 0 (1 L). The aqueous layer was extracted with EtOAc (3 x 200 mL) and the combined organics were washed with brine (2 x 200 mL), dried over anhy. Na ₂ S0 ₄ , filtered and evaporated to dryness. The residue was purified by silica gel flash chromatography eluting with a 0 to 20% EtO Ac-heptane gradient to give the desired title compound (7.9 g, 46.5%) as an off white solid. MS: 306.0 (M+H ⁺ ).

Intermediate A-7

5-Chloro-l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborol an-2-yl)-3,4- dihydroquinolin-2-one

ΓΑ1 2-(Bromomethyl)-l-chloro-3-nitro-benzene

A solution of l-chloro-2-methyl-3-nitro-benzene (25.0 g, 145.7 mmol), N-bromosuccin- imide (30.0 g, 16.8 mmol) and benzoyl peroxide (2.5 g, 10.4 mmol) in carbon tetrachloride (300 mL) was heated to 100 °C for 10 h. After TLC indicated the reaction was completed, the reaction mixture was filtered and the filtrate was concentrated in vacuo to give crude product as yellow oil (40.0 g, 100%). MS: 250.1 (M+H) ⁺ . It was used in the next step without further purification. ΓΒ1 Diethyl 2-r(2-chloro-6-nitro-phenyl)methyllpropanedioate

A solution of 2-(bromomethyl)-l-chloro-3-nitro-benzene (50.0 g, 199.6 mmol), diethyl malonate (40.0 g, 260.0 mmol) and cesium carbonate (97.5 g, 300.0 mmol) in DMF (550 mL) was stirred at 0 °C for 10 min and then at room temperature for additional 2 h. The reaction was quenched by aq. 1 N HCl (300 mL), and the aqueous layer was extracted with diethyl ether (3 x 500 mL). After filtration, the organic layer was dried over anhy. Na ₂ S0 ₄ , and concentrated in vacuo to give crude compound as yellow oil (68.0 g, 100%). MS: 330.1 (M+H) ⁺ . It was used in the next step without further purification.

TCI 5-Chloro-3,4-dihydro- lH-quinolin-2-one

A solution of diethyl 2-[(2-chloro-6-nitro-phenyl)methyl] propanedioate (20 g, 61.9 mmol), stannous chloride dehydrate (100 g, 443.0 mmol) in 6 Naq. HCl (400 mL) was heated to 130 °C for 5 h. The reaction mixture was extracted with DCM (4 x 200 mL), and washed with brine. The organic layer was dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to afford crude title compound as a yellow solid (18.0 g, 80%). MS: 181.9 (M+H) ⁺ .

ΓΡ1 5-Chloro- l-methyl-3,4-dihvdroquinolin-2-one

To a solution of 5-chloro-3,4-dihydro-lH-quinolin-2-one (16.0 g, 88.4 mmol), t-BuONa (16.0 g, 166.7 mmol) in THF (200 mL) was added methyl iodide (16.0 g, 140.4 mmol) drop wise at 0 °C. After the addition, the reaction mixture was slowly warmed up to room temperature and stirred overnight. It was then quenched with satd. aq. NaCl solution, extracted with diethyl ether (200 mL x 3), dried over anhy. Na ₂ S0 ₄ , and concentrated in vacuo. The residue was purified by silica gel flash chromatography to afford the desired title compound as a pale yellow solid (16.0 g, 82%). MS: 196.1 (M+H) ⁺ .

ΓΕ1 6-Bromo-5-chloro- 1 -methyl-3,4-dihvdroquinolin-2-one

To a solution of 5-chloro-l-methyl-3,4-dihydroquinolin-2-one (15.2 g, 77.6 mmol) in DMF (250 mL) was added bromosuccinimide (15.2 g, 85.4 mmol) portion wise at 0 °C. After the addition, the reaction mixture was allowed to warm up to room temperature and stirred overnight. The reaction was diluted with water (500 mL), extracted with diethyl ether (4 x 200 mL), dried over anhy. Na ₂ S0 _4i and concentrated in vacuo. The residue was then purified by silica gel flash chromatography to afford the desired title compound as a brown solid (16.0 g, 75%). MS: 274.9 (M+H) ⁺ .

TFl 5-Chloro-l-methyl-6-(4,4,5,5-tetramethyl-ri,3,21dioxaborolan -2-yl)-3,4-dihydro-lH- quinolin-2-one

A stirred solution of 6-bromo-5-chloro-l-methyl-3,4-dihydroquinolin-2-one (5.0 g, 18.2 mmol), bis(pinacolato)diboron (7.5 g, 29.5 mmol), PdCl ₂ (DPPF)-CH ₂ Cl ₂ (1.0 g, 1.23 mmol), KOAc (6.0 g, 61.2 mmol) in degassed dioxane (100 mL) and DMSO (10 mL) was heated to 90 °C overnight. After cooling to room temperature, the reaction mixture was filtered and the filtrate was diluted with diethyl ether, washed with brine, dried over anhy. Na ₂ S0 ₄ , and concentrated in vacuo to give a crude product, which was then purified by silica gel flash chromatography to afford desired title compound as a white solid (2.8 g, 47%). MS: 322.2(M+H) ⁺ .

Example 42

5-Fluoro-l-methyl-6-[4-[[4-(l-methylpyrazole-4-carbonyl)p iperazin-l-yl]methyl]-lH- pyrazol-3-yl]-3,4-dihydroquinolin-2-one

ΓΑ1 fe/t-Butyl 4-( 1 -methylpyrazole-4-carbonyl)piperazine- 1 -carboxylate

To a stirred solution of iert-butyl piperazine-l-carboxylate (1 g, 5.3 mmol), EDCI (1.2 g, 6.4 mmol) and HOBT (864 mg, 6.4 mmol) in DCM (30 mL) was added 1-methylpyrazole- 4-carboxylic acid (668 mg, 5.3 mmol) and DIPEA (1.4 g, 10.7 mmol). The mixture was stirred at room temperature for 3 h before it was quenched with satd. aq. NH ₄ C1 (40 mL), diluted with H ₂ 0 (100 mL), and extracted with EtOAc (3 x 30 mL). The combined organics were washed with brine (2 x 50 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (PE: EA=2: 1) to afford the desired title compound as yellow oil (900 mg, 57%). MS: 295.1 (M+H ⁺ ).

ΓΒ1 (l-Methylpyrazol-4-yl)-piperazin- 1-yl-methanone

To a stirred solution of iert-butyl 4-(l-methylpyrazole-4-carbonyl)piperazine-l- carboxylate (0.9 g, 3 mmol) in EtOAc (10 mL) was added 4N HCl in EtOAc (20 mL) and the mixture was stirred at room temperature for 30 min. The reaction mixture was filtered and the filter cake was washed with EtOAc (3 x 5 mL). The combined filtrate was washed with brine, dried over anhy. Na ₂ S04, filtered, and concentrated in vacuo to give a crude product (600 mg, 87%). MS: 195.1 (M+H ⁺ ). It was used in the next step without further purification. rCl r4-rr3-Iodo-l-(2-trimethylsilylethoxymethyl)pyrazol-4-yllmet hyllpiperazin-l-yll-(l- methylpyrazol-4-yl)methanone

To a stirred solution of 2-[[4-(bromomethyl)-3-iodo-pyrazol-l-yl]methoxy]ethyl- trimethyl-silane (1 g, 2.6 mmol, example 65[A]) in DCM (20 mL) was added (1- methylpyrazol-4-yl)-piperazin-l-yl-methanone (600 mg, 3 mmol) and DIPEA (380 mg, 3 mmol). The resulting mixture was stirred at room temperature for 3 h before it was quenched with satd. aq. NH ₄ C1 (40 mL), diluted with H ₂ 0 (50 mL), and extracted with EtOAc (3 x 20 mL). The combined organics were washed with brine (2 x 30 mL), dried over anhy. Na ₂ S0 ₄ and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (DCM: MeOH=40: l) to afford the desired title compound as yellow oil (0.8 g, 63%). MS: 531.1 (M+H ⁺ ). ΓΡ1 5-Fluoro- 1 -methyl-6- Γ4- Γ Γ4- ( 1 -methylpyrazole-4-carbonyl)piperazin- 1 -yll methyll - 1 - (2-trimethylsilylethoxymethyl)pyrazol-3-yll-3,4-dihvdroquino lin-2-one

To a stirred solution of [4-[[3-iodo-l-(2-trimethylsilylethoxymethyl)pyrazol-4-yl]met hyl]- piperazin-l-yl]-(l-methylpyrazol-4-yl)methanone (800 mg, 1.5 mmol), CS ₂ CO ₃ (970 mg, 3 mmol) and 5-fluoro-l-methyl-6-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan -2-yl)-3,4- dihydro-lH-quinolin-2-one (450 mg, 1.5 mmol) (intermediate A-6) in dioxane/H ₂ 0 (15: 1, 32 mL) was added PdCl ₂ (DPPF) (110 mg, 0.15 mmol) under N ₂ . The reaction mixture was then heated to 80 °C for 5 h before it was quenched with satd. aq. NH ₄ CI (40 mL), diluted with H ₂ 0 (50 mL), and extracted with EtOAc (3 x 20 mL). The combined organics were washed with brine (2 x 20 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (DCM: MeOH = 40: 1) to afford the desired title compound as yellow oil (260 mg, 29%). MS: 582.2 (M+H ⁺ ).

ΓΕ1 5-Fluoro- 1 -methyl-6- Γ4- ΓΓ4- ( 1 -methylp yrazole-4-carbonyl)piperazin- 1 -yll methyll - 1 H- pyrazol-3-yll-3,4-dihydroquinolin-2-one

A solution of 5-fluoro-l-methyl-6-[4-[[4-(l-methylpyrazole-4-carbonyl)pipe razin-l- yl]methyl]- l-(2-trimethylsilylethoxymethyl)pyrazol-3-yl]-3,4-dihydroqui nolin-2-one (250 mg, 0.43 mmol) in TFA (10 mL) was stirred at room temperature for 1 h. The reaction mixture was quenched by the addition of satd. aq. NaHC0 ₃ solution (100 mL), diluted with H ₂ 0 (30 mL), and extracted with EtOAc (3 x 20 mL). The combined organics were washed with brine (2 x 20 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (DCM: MeOH = 40: 1) to afford the desired title compound as yellow oil (60 mg, 30%). MS: 452.2 (M+H ⁺ ).

Example 43

5-Chloro-l-methyl-6-[4-[[4-(l-methylpyrazole-4-carbonyl)p iperazin-l-yl]methyl]-lH- pyrazol-3-yl]-3,4-dihydroquinolin-2-one

In analogy to the procedure described for the synthesis of example 42, 5-chloro-l-methyl- 6-(4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-one (intermediate A-7) was used in step 42[D] to afford the desired title compound (4.2 mg, 3.5%) as a white solid. MS: 468.2 (M+H ⁺ ).

Example 44

8-Chloro-l-methyl-6-[4-[[4-(l-methylpyrazole-4-carbonyl)p iperazin-l-yl]methyl]-lH- pyrazol-3-yl]-3,4-dihydroquinolin-2-one

In analogy to the procedure described for the synthesis of example 42, 8-chloro- l-methyl- 6-(4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-one (intermediate A-5) was used in step 42[D] to afford the desired title compound (6.2 mg, 4.2%) as a white solid. MS: 468.2 (M+H ⁺ ).

Example 45

(-)-6-r4-rr(3R)-4-Acetyl-3-methyl-piperazin-l-yl1methyl1- lH-pyrazol-3-yl1-5-fluoro-l- methyl-3,4-dihvdroquinolin-2-one

ΓΑ1 2-rr4-(Chloromethyl)-3-iodo-pyrazol- l-yllmethoxylethyl-trimethyl-silane

To a stirred solution of [3-iodo- l-(2-trimethylsilylethoxymethyl)pyrazol-4-yl]methanol (1.0 g, 2.8 mmol, example 53[C]) and DIPEA (2.0 g, 15.5 mmol) in DCM (20 mL), was added MsCl (450 mg, 3.9 mmol) drop wise . The mixture was stirred at room temperature for 2 h before the solvent was evaporated to give a crude product (3.0 g crude, 100% yield). MS: 372.1 (M+H ⁺ ). It was used in the next step without further purification.

ΓΒ1 (-)-tert-Butyl (2R)-4-rr3-iodo- l-(2-trimethylsilylethoxymethyl)pyrazol-4-yllmethyll-2- methyl-piperazine- 1 -carboxylate

A solution of 2-[[4-(chloromethyl)-3-iodo-pyrazol- l-yl]methoxy]ethyl-trimethyl-silane (3.0 g crude, 2.8 mmol), (-)-ieri-butyl (2R)-2-methylpiperazine- 1-carboxylate (900 mg, 4.5 mmol) and CS ₂ CO ₃ (3.25 g, 10 mmol) in DMF (15 mL) was stirred at room temperature for 2 h. It was then quenched by the addition of water (20 mL), and extracted with Et ₂ 0 (3 x 40mL). The combined organics were dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated under vacuum to give a crude product, which was purified by silica gel flash

chromatography (DCM: MeOH=20: l) to afford the desired title compound (1.3 g 86% yield) as colorless oil. MS: 537.2 (M+H ⁺ ).

TCI (-)-fe -Butyl (2R)-4-rr3-(5-fluoro- l-methyl-2-oxo-3,4-dihvdroquinolin-6-yl)- l-(2- trimethylsilylethoxymethyl)pyrazol-4-yllmethyll -2-methyl-piperazine- 1-carboxylate

A stirred solution of (-)-tert-butyl (2R)-4-[[3-iodo-l-(2- trimethylsilylethoxymethyl)pyrazol-4-yl] methyl] -2-methyl-piperazine- 1-carboxylate ( 400 mg, 0.75 mmol), 5-fluoro- l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-3,4 - dihydroquinolin-2-one (intermediate A-6, 300 mg, 0.98 mmol), PdCl ₂ (DPPF)-CH ₂ Cl ₂ (50 mg) and Na ₂ C0 ₃ (212 mg, 2.0 mmol) in dioxane (25 mL) and water (2.5 mL) was heated at 110°C for 45 min. The mixture was then diluted with water (10 mL), extracted with EtOAc (4 x 20 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (DCM:

MeOH=20: l) to afford the desired title compound (500 mg, 79% yield) as yellow oil. MS: 587.4 (M+H ⁺ ).

ΓΡ1 (-)-5-Fluoro-l-methyl-6-r4-rr(3R)-3-methylpiperazin-l-yllmet hyll-lH-pyrazol-3-yll- 3,4-dihvdroquinolin-2-one

A solution of (-)-ieri-butyl (2R)-4-[[3-(5-fluoro-l-methyl-2-oxo-3,4-dihydroquinolin-6- yl)- 1- (2-trimethylsilylethoxymethyl)pyrazol-4-yl] methyl] -2-methyl-piperazine- 1- carboxylate (350 mg, 0.59 mmol) in DCM/TFA (1: 1, 10 mL) was stirred at room temperature for 2 h before the solvent was evaporated to dryness to afford a crude product (360 mg, 100%) as brown oil. MS: 358.3 (M+H ⁺ ). It was used in the next step without further purification.

ΓΕ1 (-)-6-r4-rr(3R)-4-Acetyl-3-methyl-piperazin-l-yllmethyll-lH- pyrazol-3-yll-5-fluoro- l-methyl-3,4-dihydroquinolin-2-one

To a solution of (-)-5-fluoro-l-methyl-6-[4-[[(3R)-3-methylpiperazin-l-yl]met hyl]-lH- pyrazol-3-yl]-3,4-dihydroquinolin-2-one crude 0.43 mmol) and Ac ₂ 0 (56 mg, 0.5 mmol) in DCM (3 mL) was added DIPEA (260 mg, 2.0 mmol) at 0 °C. The mixture was stirred at room temperature for 2 h before the solvent was evaporated under reduced pressure. The residue was subjected to prep-HPLC separation to afford the desired title compound (10 mg, 6%) as a white solid. MS: 400.2 (M+H ⁺ ).

Example 46

(-)-5-Fluoro-l-methyl-6-[4-[[(3R)-3-methyl-4-(l-methylpyr azole-4- carbonyl)piperazin-l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydro quinolin-2-one

To a stirred solution of (-)-5-fluoro-l-methyl-6-[4-[[(3R)-3-methylpiperazin-l-yl]met hyl]- lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one (Example 45[D], crude 0.43 mmol), 1- methylpyrazole-4-carboxylic acid (63 mg, 0.50 mmol), and HATU (228 mg, 0.60 mmol) in DCM (4 mL) was added DIPEA (1.29 g,10 mmol) drop wise at 0 °C. The resulting reaction mixture was then washed with satd. aq. NaHC0 ₃ solution, and the organic layer was dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated under vacuum to give a crude product, which was purified by prep-HPLC to afford the desired title compound (9 mg, 5% yield) as a white solid. MS: 466.2 (M+H ⁺ ).

Example 47

(-)-6-[4-[[(3R)-4-Acetyl-3-methyl-piperazin-l-yl]methyl]- lH-pyrazol-3-yl]-5-chloro-l- methyl-3,4-dihydroquinolin-2-one

In analogy to the procedure described for the synthesis of example 45, 5-chloro-l-methyl- 6-(4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-one (intermediate A-7) was used in step 45[C] to afford the desired title compound ( 6 mg, 9.1 %) as a white solid. MS: 416.2 (M+H ⁺ ).

Example 48

(-)-5-Chloro-l-methyl-6-[4-[[(3R)-3-methyl-4-(l-methylpyr azole-4- carbonyl)piperazin-l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydro quinolin-2-one

In analogy to the procedures described for the synthesis of examples 45 and 46, 5-chloro- l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-3,4 -dihydroquinolin-2-one (intermediate A-7) and l-methylpyrazole-4-carboxylic acid were used respectively to give the desired title compound (5 mg, 6.8%) as a white solid. MS: 482.2 (M+H ⁺ ).

Example 49 (-)-6-[4-[[(3R)-4-Acetyl-3-methyl-piperazin-l-yl]methyl]-lH- pyrazol-3-yl]-l-methyl- 3,4-dihydroquinolin-2-one

In analogy to the procedure described for the synthesis of example 45, l-methyl-6- (4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-one (Intermediate A- 3) was used in step 45[C] to afford the desired title compound (8 mg, 7.8%) as a white solid. MS: 382.2 (M+H ⁺ ).

Example 50

(-)-l-Methyl-6-[4-[[(3R)-3-methyl-4-(l-methylpyrazole-4-c arbonyl)piperazin-l- yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one

In analogy to the procedures described for the synthesis of examples 45 and example 46, l-methyl-6-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-3,4 -dihydroquinolin-2-one (Intermediate A-3) and l-methylpyrazole-4-carboxylic acid were used respectively to give the desired title compound (9 mg, 6.1%) as a white solid (9 mg, 6.1%). MS: 448.2

(M+H ⁺ ). Example 51 (+)-5-Chloro-l-methyl-6-[4-[[(2R)-2-methyl-4-(l-methylpyrazo le-4- carbonyl)piperazin-l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydro quinolin-2-one

In analogy to the procedures described for the synthesis of examples 45 and example 46, (+)-tert-butyl (3R)-3-methylpiperazine-l-carboxylate, 5-chloro-l-methyl-6-(4,4,5,5- tetramethyl-l,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-o ne (intermediate A-7) and 1- methylpyrazole-4-carboxylic acid were used respectively to give the desired title compound (4.5 mg, 4.5%) as a white solid. MS: 482.2 (M+H ⁺ ).

Example 52

(+)-5-Fluoro-l-methyl-6-[4-[[(2R)-2-methyl-4-(l-methylpyr azole-4- carbon l)piperazin-l-yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinoli n-2-one

In analogy to the procedures described for the synthesis of examples 45 and 46, (+)-tert- butyl (3R)-3-methylpiperazine-l-carboxylate, 5-fluoro-l-methyl-6-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)-3,4-dihydroquinolin-2-one (intermediate A-6) and 1- methylpyrazole-4-carboxylic acid were used respectively to give the desired title compound (5.3 mg, 5.1 %) as a white solid. MS: 466.2 (M+H ⁺ ).

Example 53

5- Chloro-l-methyl-6-[4-[[2-(l-methylpyrazole-4-carbonyl)-2,6-d iazaspiro[3.3]heptan-

6- yl]methyl]-lH-pyrazol-3-yl]-3,4-dihydroquinolin-2-one

To a solution of ethyl 3-amino-lH-pyrazole-4-carboxylate (40 g, 0.258 mol) in MeCN (40 mL) was added isopentyl nitrite (36 g, 0.31mol) and CH ₂ I ₂ (207 g, 0.774 mol) at 0 °C. The resulting mixture was stirred at 0 °C for 20 min and then heated to 100 °C for 12 h. After cooling to room temperature, the mixture was concentrated in vacuo and the residue was diluted with water and extracted with EtOAc (3 x 300 mL). The combined organics were washed with brine (3 x 200 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (PE: EA=2: 1) to give the desired title compound (40 g, 58%) as a yellow solid. MS: 267.1 (M+H ⁺ ).

ΓΒ1 Ethyl 3-iodo- l-(2-trimethylsilylethoxymethyl)pyrazole-4-carboxylate

To a solution of ethyl 3-iodo-lH-pyrazole-4-carboxylate (40 g, 150 mmol) in THF (500 mL) was added NaH (7.2 g, 180 mmol) portion wise at 0 °C. The resulting mixture was stirred at 0 °C for 30 min before SEMC1 (30 g, 180 mmol) was added drop wise. The mixture was then allowed to warm up to room temperature and stirred for 3 h before it was quenched with satd. aq. NH ₄ C1 (200 mL), and extracted with EtOAc (3 x 200 mL). The combined organics were washed with brine (3 x 200 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (PE: EA=2: 1) to afford the desired title compound (50 g, 84%) as a white solid. MS: 397.1 (M+H ⁺ ).

TCI r3-Iodo-l-(2-trimethylsilylethoxymethyl)pyrazol-4-yllmethano l

To a stirred solution of ethyl 3-iodo-l-(2-trimethylsilylethoxymethyl)pyrazole-4- carboxylate (50 g, 126 mmol) in THF (600 mL) was added DIBAL (379 mL, 379 mmol) at -78 °C and the resulting mixture was stirred at -78 °C for 3 h before was warmed up to room temperature and quenched by the addition of a satd. Seignette salt solution (600 mL). After dilution with H ₂ 0 (1 L), the aqueous layer was extracted with EtOAc (3 x 300 mL). The combined organics were washed with brine (3 x 200 mL), dried over anhy. Na ₂ S0 ₄ filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (PE: EA=2: 1) to afford the desired title compound (28 g, 63%) as yellow oil. MS: 355.1 (M+H ⁺ ).

ΓΡ1 3-Iodo- 1 -(2-trimethylsilylethoxymethyl)pyrazole-4-carbaldehyde

To a solution of [3-iodo-l-(2-trimethylsilylethoxymethyl)pyrazol-4-yl]methano l (7 g, 17.7 mmol) in DCM (100 mL) was added DMP (Dess-Martin-Periodinane) (19 g, 35.4 mmol) at room temperature and the mixture was stirred for 3 h. After TLC (petroleum /EtOAc = 3:1, Rf = 0.6) showed the completion of reaction, the reaction mixture was quenched with satd. NaHC0 ₃ solution, and extracted with DCM. The organic layer was dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel falsh chromatography (PE: EA=2: 1) to afford the desired title compound (6.5 g, 92%) as a white solid. MS: 353.1 (M+H ⁺ ). ΓΕ1 fe/t-Butyl 6-rr3-iodo-l-(2-trimethylsilylethoxymethyl)pyrazol-4-yllmeth yll-2,6- diazas iror3.31heptane-2-carboxylate

A solution of 3-iodo-l-(2-trimethylsilylethoxymethyl)pyrazole-4-carbaldehy de (6.5 g, 18.5 mmol), ie/t-butyl 2,6-diazaspiro[3.3]heptane-2-carboxylate (3.6 g, 18.5 mmol),

NaBH(OAc) ₃ (7.8 g, 37 mmol) and Et ₃ N (3.7 g, 37 mmol) in CHC1 ₃ (500 mL) was stirred at room temperature for 20 h. After TLC (DCM /MeOH = 20: 1, R _f = 0.5) shows the completion of the reaction, the reaction mixture was washed with satd. aq. NaHC0 ₃ and the organic layer was washed with brine, dried over anhy. Na ₂ S0 _4i filtered, and concentrated in vacuo to afford the desired crude compound (5.6 g, 57%) as yellow oil. It was used in the subsequent step without further purification. MS: 535.1 (M+H ⁺ ).

TF1 fe/ -Butyl 6-IT3-(5-chloro- l-methyl-2-oxo-3,4-dihvdroquinolin-6-yl)- 1-(2- trimethylsilylethoxymethyl)pyrazol-4-yllm

To a solution of tert-b tyl 6-[[3-iodo-l-(2-trimethylsilylethoxymethyl)pyrazol-4- yl]methyl]-2,6-diazaspiro[3.3]heptane-2-carboxylate (100 mg, 0.187 mmol), CS ₂ CO ₃ (100 mg, 0.31 mmol) and 5-chloro-l-methyl-6-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan -2-yl)- 3,4-dihydro-lH-quinolin-2-one (66 mg, 0.205 mmol) (intermediate A-7) in dioxane/ H ₂ 0 (5: 1, 4 mL) was added PdCl ₂ (DPPF)-CH ₂ Cl ₂ (20 mg) under N ₂ . The resulting solution was heated to 100 °C for 1 h. After cooling to room temperature, the reaction was quenched with satd. aq. NH ₄ CI solution (40 mL), diluted with H ₂ 0 (50 mL), and extracted with EtOAc (3 x 20 mL). The combined organics were washed with brine (2 x 20 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (DCM: MeOH = 40: 1) to afford the desired title compound (80 mg, 71.4%) as yellowish oil. MS: 602.1 (M+H ⁺ ).

TGI 5-Chloro-6-r4-(2,6-diazaspiror3.31heptan-2-ylmethyl)-lH-pyra zol-3-yll-l-methyl-3,4- dihvdroquinolin-2-one

To a solution of tert-b tyl 6-[[3-(5-chloro-l-methyl-2-oxo-3,4-dihydroquinolin-6-yl)-l-( 2- trimethylsilylethoxymethyl)pyrazol-4-yl]methyl]-2,6-diazaspi ro[3.3]heptane-2-carboxylate (80 mg, 0.132 mmol) in DCM (2.0 mL) was added TFA (3 mL) at room temperature. The reaction mixture was stirred for 30 min before it was concentrated in vacuo to give a crude product (100 mg) as yellow oil. MS: 372.1 (M+H ⁺ ). It was used in the subsequent step without further purification. MS: 372.1 (M+H ⁺ ).

ΓΗ1 5-Chloro-l-methyl-6-r4-rr2-(l-methylpyrazole-4-carbonyl)-2,6 -diazaspiror3.31heptan- 6- llmethyll-lH-pyrazol-3-yll-3,4-dihydroquinolin-2-one

To a stirred solution of 5-chloro-6-[4-(2,6-diazaspiro[3.3]heptan-2-ylmethyl)-lH-pyra zol- 3-yl]-l-methyl-3,4-dihydroquinolin-2-one (100 mg, 0.133 mmol) and HATU (20 mg, 0.145 mmol) in DCM (5 mL) was added l-methylpyrazole-4-carboxylic acid (67 mg, 0.43 mmol) and DIPEA (0.9 mL). The resulting reaction mixture was stirred at room

temperature for 1 h before it was quenched with satd. aq. NH ₄ C1 (20 mL), diluted with H ₂ 0 (50 mL), and extracted with DCM (3 x 10 mL). The combined organics were washed with brine (2 x 20 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by silica gel flash chromatography (DCM: MeOH=40: 1) to afford the desired title compound (20 mg, 31.7%) as colorless oil. MS: 480.1 (M+H ⁺ ).

Example 54 5-Chloro-6-[4-[(2-ethylsulfonyl-2,6-diazaspiro[3.3]heptan-6- yl)methyl]-lH-pyrazol-3- yl]-l-methyl-3,4-dihydroquinolin-2-one

To a solution of 5-chloro-6-[4-(2,6-diazaspiro[3.3]heptan-2-ylmethyl)-lH-pyra zol-3-yl]-l- methyl-3,4-dihydroquinolin-2-one (Example 53[G], 100 mg, 0.133 mmol) in DCM (5 mL) was added ethanesulfonyl chloride (19 mg, 0.143 mmol) and DIPEA (1 mL). The resulting reaction mixture was stirred at room temperature for 1 h before it was quenched with satd. aq. NH ₄ C1 (20 mL), diluted with H ₂ 0 (50 mL), and extracted with DCM (3 x 10 mL). The combined organics were washed with brine (2 x 20 mL), dried over anhy. Na ₂ S0 ₄ , filtered, and concentrated in vacuo to give a crude product, which was purified by prep-HPLC to afford the desired title compound (15 mg, 24.4%) as light yellowish oil. MS: 464.1 (M+H ⁺ ).

The following compounds listed in Table 3 were prepared in analogy to the procedures described for the preparation of examples 53 or 54 using appropriate starting materials.

Name Example MS

Examp

Reactants

le

Aspect Procedures (M+H ⁺ ) Name Example MS

Examp

Reactants

le

Aspect Procedures (M+H ⁺ )

5-Fluoro-l- methyl-6-

5-Fluoro-l-methyl-6-[4-[[2-(l-

(4,4,5,5- methylpyrazole-4-carbonyl)-2,6- tetramethyl- diazaspiro[3.3]heptan-6-yl]methyl]-

1,3,2- lH-pyrazol-5-yl]-3,4- dioxaborolan- dihydroquinolin-2- one

2-yl)-3,4-

55 53 464.1 di ydroquinoli

n-2-one

(intermediate

A-6) and 1- methylpyrazol

colorless oil

e-4-carboxylic

acid

6-[4-[(2-Ethylsulfonyl-2,6- 1- Methyl-6- diazaspiro[3.3]heptan-6-yl)methyl]- (4,4,5,5- lH-pyrazol-5-yl]-l-methyl-3,4- tetramethyl- dihydroquinolin-2- one 1,3,2- dioxaborolan-

2- yl)-3,4-

56 54 430.1 dihydroquinoli

n-2-one

(intermediate

A-3) and

ethanesulfonyl

colorless oil chloride Name Example MS

Examp

Reactants

le

Aspect Procedures (M+H ⁺ )

1- Methyl-6-

6-[4-[(2-Isopropylsulfonyl-2,6- (4,4,5,5- diazaspiro[3.3]heptan-6-yl)methyl]- tetramethyl- lH-pyrazol-5-yl]-l-methyl-3,4- 1,3,2- dihydroquinolin-2- one dioxaborolan-

2- yl)-3,4-

57 dihydroquinoli 54 444.1 n-2-one

(intermediate

A-3) and

propane-2- sulfonyl

colorless oil

chloride

1- Methyl-6- l-Methyl-6-[4-[[2-(4- (4,4,5,5- methylpyridine-3-carbonyl)-2,6- tetramethyl- diazaspiro[3.3]heptan-6-yl]methyl]- 1,3,2- lH-pyrazol-5-yl]-3,4- dioxaborolan- dihydroquinolin-2- one

2- yl)-3,4-

58 dihydroquinoli 53 457.1 n-2-one

(intermediate

A-3) and 4- methyl- pyridine-3- colorless oil

carboxylic acid Name Example MS

Examp

Reactants

le

Aspect Procedures (M+H ⁺ )

l-Methyl-6-[4-[[2-(l- 1- Methyl-6- methylimidazole-2-carbonyl)-2,6- (4,4,5,5- diazaspiro[3.3]heptan-6-yl]methyl]- tetramethyl- lH-pyrazol-5-yl]-3,4- 1,3,2- dihydroquinolin-2- one dioxaborolan-

2- yl)-3,4-

63 dihydroquinoli 53 446.1 n-2-one

(intermediate

A-3) and 1- methyl- imidazole-2- white foam carboxylic acid

1- Methyl-6-

6-[4-[[2-(3-Chloropyridine-2- (4,4,5,5- carbonyl)-2,6- tetramethyl- diazaspiro[3.3]heptan-6-yl]methyl]- 1,3,2- lH-pyrazol-5-yl]-l-methyl-3,4- dioxaborolan- dihydroquinolin-2- one

2- yl)-3,4-

64 dihydroquinoli 53 477.1 n-2-one

(intermediate

A-3) and 3- chloro- pyridine-2- white foam

carboxylic acid

Example A

A compound of formula (I) can be used in a manner known per se as the active ingredient for the production of tablets of the following composition: Per tablet

Active ingredient 200 mg

Microcrystalline cellulose 155 mg

Corn starch 25 mg

Talc 25 mg

Hydroxypropylmethylcellulose 20 mg

425 mg

Example B A compound of formula (I) can be used in a manner known per se as the active ingredient for the production of capsules of the following composition:

Per capsule

Active ingredient 100.0 mg

Corn starch 20.0 mg

Lactose 95.0 mg

Talc 4.5 mg

Magnesium stearate 0.5 mg

220.0 mg