The field of the invention

The subject of our invention relates to a new polymorphic form of the pharmaceutical active substance idelalisib, to a method for its production, to pharmaceutical preparations containing the new form, as well as the therapeutic use of all these.

The state of the art

The structural formula of 3-phenyl-5-fluoro-2-[l(5)-(9H-purin-6-ylamino)propyl]quinazo lin- 4(3H)-one, with the international non-proprietary name idelalisib is the following:

Idelalisib is a phosphoinositide-3-kinase (PI3K) enzyme inhibitor. The phosphoinositide-3- kinase enzymes participate in the regulation of numerous cell functions (growth regulation, metabolism, translation initiation). Idelalisib has been registered for the treatment of various forms of leukaemia. The production of idelalisib was first presented in international patent application number WO 2005113556. The solid material obtained following column chromatography purification was dissolved in ethanol, and then the solution was evaporated. On the basis of element analysis the composition of the light yellow solid material obtained in this way is idelalisib · EtOH · 0.4 H ₂ 0. The inventors do not present analytical results serving to characterise the crystallography of the solid phase.

International patent application number WO 2013134288 presents the seven forms of idelalisib (Form I, II, III, IV, V, VI and VII), their production, their use for the production of pharmaceutical preparations and their medical use. The characteristic x-ray powder diffractogram of each form was disclosed (the unit cell parameters of Forms I, II, III, IV, and V were also given), and also the individual crystal forms were also examined using thermal analysis methods (TG, DSC). Form I and Form II are solvent-free crystalline forms, and all of the other crystalline forms are solvates of the idelalisib base. The Form I polymorph is crystalized from a methanol-water mixture, with the temperature of the mixture being precisely regulated. The Form II polymorph is obtained by grinding the Form I polymorph, optionally with related solvent suspension or by the compression of the Form I polymorph. In other words, the Form I polymorph may be partially transformed into Form II in the course of certain preparation development operations (e.g. tableting) under generally used conditions. The joint presence of the two forms in the pharmaceutical preparation, presumably in varying proportions with respect to the specific characteristics of the pharmaceutical technology operations performed, in all events represents technological and quality control difficulties.

International patent application number WO 2015014315 presents ten forms of idelalisib, of which four are solvent-free crystalline forms, and the others some form of solvate. Among the solvent-free crystalline forms two are the same as the Form I and Form II polymorphs presented in patent application number WO 2013134288. One of the most important advantages of the two other forms (Form IV and Form VIII) is mentioned as being low hygroscopicity: in the case of Form IV the amount of water bound from the environmental moisture content is 0.3% while this amount in the case of Form VIII is 0.84% in the 20-80% relative humidity range.

International patent application number WO 2015092810 presents the amorphous form of idelalisib. In the knowledge of the numerous crystalline forms, it is obvious that the production of the amorphous form is only possible under special, strictly controlled conditions. Another disadvantage of the amorphous form in such cases is that during storage it may become transformed into one of the more thermodynamically stable crystalline forms . According to the examples described in the patent application, the amorphous form was only obtained using spray drying technology, or by evaporation of an idelalisib solution in the presence of a pharmaceutical excipient (polyvinylpyrrolidone, hydroxypropyl methylcellulose).

A brief description of the invention The characteristics of solid phase pharmaceutical active substances have a fundamental influence on the determinant features of the pharmaceutical preparations made from them, such as release with its effect on bioavailability, stability, and also determine the range of pharmaceutical technology procedures that may be used. Therefore, the production of the solid phase forms of pharmaceutical active substances (polymorphs, salts, solvates, complexes) and the analysis of their characteristics are essential parts of the medicine development process.

The objective of the invention is to overcome the disadvantages of the known methods and elaborate a method for the production of new polymorphs of the idelalisib active substance that may be simply and economically implemented at industrial scales, that maintains its homogenous polymorphic form during pharmaceutical technology operations (grinding, granulations, tableting) and during the stability tests required when developing pharmaceutical preparations.

The idelalisib base Form E according to the invention has numerous preferable properties as compared to earlier idelalisib forms. Prominent among these is that during pressing it maintains its crystalline form. Furthermore, it has a preferably low degree of hygroscopicity. An especially preferable feature of the idelalisib base Form E according to the invention is that the stability exhibited in the course of pressing and especially low hygroscopicity are both characteristic of it at the same time. Preferably low hygroscopicity in a wide range of relative humidity is characteristic of idelalisib base Form E. These characteristics together make the crystalline form of the idelalisib base Form E especially preferable for use as a pharmaceutical active substance as compared to the earlier idelalisib forms.

Figures

Figure 1: the x-ray powder diffractogram of the idelalisib base Form E Figure 2: The effect of compression on the crystal structure of the idelalisib base Form E. The upper curve shows the x-ray powder diffractogram of a sample of an idelalisib Form E tablet produced by high-pressure compression, while the lower curve depicts the x-ray powder diffractogram of a sample not subjected to compression.

Figure 3: The effect of compression on the crystal structure of the idelalisib base Form I. The upper curve: the x-ray powder diffractogram of idelalisib Form II. The lower curve: the x-ray powder diffractogram of idelalisib Form I. The middle curve shows the x-ray powder diffractogram of idelalisib Form I subjected to pressure.

Figure 4: The 25 °C water sorption isotherms of Form I and Form II of the patent application number WO 2013134288 and of the Form E idelalisib base. The figure depicts the percentage equilibrium moisture content as a function of relative humidity.

Figure 5: The x-ray powder diffractogram of idelalisib base acetonitrile solvate (Form E-S)

A detailed description of the invention

More specifically the subject of the invention relates to the crystalline Form E of the idelalisib base, the characteristic x-ray powder diffraction peaks of which are the following: 2Θ (±0.2° 2Θ): 11.56; 12.80; 14.96; 20.85. More specifically it may be characterised by the following x- ray powder diffraction peaks: 2Θ (±0.2° 2Θ): 11.56; 12.80; 14.96; 15.70; 18.24; 19.34; 20.25; 20.85; 22.73; 23.91; 32.42. Even more specifically it may be characterised by the following x- ray powder diffraction peaks: 2Θ (±0.2° 2Θ): 11.56; 11.73; 12.80; 13.71; 14.96; 15.55; 15.70; 17.57; 18.24; 19.34; 20.25; 20.47; 20.85; 22.18; 22.52; 22.73; 23.26; 23.62; 23.91; 24.16; 24.41; 24.86; 25.54; 25.85; 26.35; 27.04; 27.24; 27.40; 30.63; 30.86; 31.44; 31.71; 32.01; 32.42; 32.81; 33.67. The characteristic x-ray powder diffractogram of Form E may be seen in figure 1, and the 1% or greater intensity peaks are summarised in table 1.

Table 1 The x-ray powder diffraction data of idelalisib base Form E

( relative intensities > 1%)

Peak 2Θ (°) d (A) Relative intensity (%)

8 17.57 5.04 4

9 18.24 4.86 10

10 19.34 4.59 7

11 20.25 4.38 19

12 20.47 4.34 2

13 20.85 4.26 100

14 22.18 4.00 4

15 22.52 3.94 2

16 22.73 3.91 12

17 23.26 3.82 1

18 23.62 3.76 1

19 23.91 3.72 17

20 24.16 3.68 5

21 24.41 3.64 1

22 24.86 3.58 4

23 25.54 3.49 6

24 25.85 3.44 11

25 26.35 3.38 8

The resistance of the crystal structure of the new idelalisib base form (Form E) according to our invention to mechanical stress was investigated with compression tests. The set pressure value (approx. 50 MPa) is comparable to the pressure values usually used when producing tablets. The x-ray powder diffractogram of a sample from a tablet of idelalisib base Form E produced using high pressure is presented in figure 2. It can be seen that the diffractogram is the same as that of the initial sample (before compression), in other words the pressing did not result in solid phase transformation in the sample. A similar experiment was performed with idelalisib Form I according to patent application number WO 2013134288, the results can be seen in figure 3. In this case, in accordance with that stated in the application referred to, the diffraction peaks (indicated with arrows in figure 3) characteristic of idelalisib base Form II appeared in the diffractogram of the tablet obtained, in other words the sample partially transformed into a crystal structure that is not homogenous. On the basis of this idelalisib base Form I is less resistant to mechanical stress, the sample suffered solid phase transformation due to the effect of high-pressure compression. The phenomenon experienced in the case of Form I is not preferable, as during preparation formulation (e.g. tableting) the active substance is subjected to pressure of this order, as a result of which the crystal structure of the active substance in the preparation will not be homogenous.

The water sorption isotherms of the idelalisib base Form E recorded at 25 °C are presented in figure 4. It can be seen that the substance does not display hygroscopicity: in the relative humidity range of 0-85% the maximum water absorption is under 0.15%. According to the measurement results the hygroscopicity of idelalisib base Form E in the examined range is lower than that of Form I and Form II according to patent application number WO 2013134288, and is also preferably lower than that of the two new solvent-free forms (Form IV and Form VIII) presented in patent application number WO 2015014315. The lower hygroscopicity experienced in the case of Form E is also preferable from the points of view of stability and preparation formulation.

More specifically the subject of our invention also relates to the acetonitrile solvate of the crystalline idelalisib base (Form E-S), the significance of which is that the idelalisib Form E crystalline form according to the invention may be produced via this.

The characteristic x-ray powder diffraction peaks characteristic of the Form E-S acetonitrile solvate are the following: 2Θ (±0.2° 2Θ): 6.81; 7.94; 8.65; 12.47; 20.99. More specifically it can be characterised by the following x-ray powder diffraction peaks: 2Θ (±0.2° 2Θ): 6.81;

7.94; 8.65; 11.99; 12.47; 13.13; 15.17; 20.99; 25.09; 29.02. Even more specifically it can be characterised by the following x-ray powder diffraction peaks: 2Θ (±0.2° 2Θ): 6.81; 7.94; 8.65;

11.99; 12.47; 13.13; 13.57; 15.17; 15.93; 17.02; 17.20; 17.85; 18.46; 19.07; 19.23; 19.45; 20.27; 20.99; 21.92; 22.11; 22.45; 22.74; 23.07; 23.49; 24.04; 24.25; 25.09; 25.79; 26.37;

27.00; 27.44; 27.68; 28.10; 28.58; 29.02; 29.44; 29.86; 30.29; 30.56; 31.14; 32.04; 32.45; 32.71; 33.11; 34.03; 34.65. The characteristic x-ray powder diffractogram of this may be seen in figure 5, and the 2% or greater intensity peaks are summarised in the following table 2.

Table 2 The x-ray powder diffraction data of the idelalisib base acetonitrile solvate (Form E-

S) (relative intensities > 2%)

Peak 2Θ (°) d (A) Relative intensity (%)

22 22.74 3.91 30

23 23.07 3.85 20

24 23.49 3.78 21

25 24.04 3.70 96

26 24.25 3.67 29

27 25.09 3.55 48

28 25.79 3.45 17

29 26.37 3.38 44

30 27.00 3.30 16

31 27.44 3.25 19

32 27.68 3.22 16

33 28.10 3.17 13

34 28.58 3.12 9

35 29.02 3.07 22

36 29.44 3.03 10

37 29.86 2.99 4

38 30.29 2.95 9

39 30.56 2.92 2

40 31.14 2.87 2

41 32.04 2.79 8

42 32.45 2.76 7

43 32.71 2.74 9

44 33.11 2.70 10

45 34.03 2.63 4

46 34.65 2.59 3 The subject of the invention also relates to a method for the production of the Form E-S acetonitrile solvate of 3-phenyl-5-fluoro-2-[l(5)-(9H-purin-6-ylamino)propyl]quinazo lin- 4(3H)-one (idelalisib) in such a way that 3-phenyl-5-fluoro-2-[l(5)-(9H-purin-6- ylamino)propyl]quinazolin-4(3H)-one is dissolved in acetonitrile, the solution is stirred, the crystalline substance precipitated during stirring is the acetonitrile solvate Form E-S of 3- phenyl-5-fluoro-2-[l(5)-(9H-purin-6-ylamino)propyl]quinazoli n-4(3H)-one.

The subject of the invention also relates to a method for the production 3-phenyl-5-fluoro-2- [l(5)-(9H-purin-6-ylamino)propyl]quinazolin-4(3H)-one (idelalisib) Form E, in such a way that the Form E-S acetonitrile solvate form according to the invention is dried. Furthermore, the subject of the invention relates to a pharmaceutical preparation containing idelalisib Form E and pharmaceutically acceptable additives.

The subject of the invention also relates to a medical preparation containing a therapeutically effective amount of idelalisib base Form E according to the invention and, optionally, one or more excipients used in the production of medical preparations. Furthermore, a method for the production of a medical preparation, characterised by that the idelalisib base Form E according to the invention is mixed with an appropriate amount of medically acceptable carrier and, optionally, other medically suitable excipients, and is then placed in a galenic formulation.

The medial preparations according to the invention are preferably administered orally. Orally administered preparations may include, for example, tablets, capsules, dragees, solutions, elixirs, suspensions and emulsions.

The medical preparations according to the invention may contain the usual medical carriers and/or excipients. Carriers include, for example, magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatine, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, low melting point wax, PEG, cocoa butter, etc. In the case of capsules it is frequently the material of the capsule that serves as the carrier and so in such a case there is no need for a separate carrier. Orally administered preparations also include sachets and lozenges. Tablets, powders, capsules, pills, sachets and lozenges are especially suitable solid preparation forms. An especially preferable medical preparation is the film-coated tablet containing idelalisib in the tablet core. According to a preferable embodiment of the method the tablet core contains the following excipients: microcrystalline cellulose, hydroxypropyl cellulose (E463), croscarmellose-sodium, carboxymethyl starch sodium and magnesium stearate. The tablets may be produced by mixing the active substance with carrier materials with suitable characteristics in the appropriate ratio, and by pressing the desired shape and size tablets from the mixture.

Powders are produced by mixing the finely powdered active substance with finely powdered carrier material. Liquid preparations include solutions, suspensions and emulsions, from which, optionally, the active substance is released in a delayed way.

The medical preparations according to the invention are preferably produced in the form of dose units. The dose units contain the desired amount of active substance. The dose units may be distributed in packaged form, which contains separated amounts of the preparations (e.g. packaged tablets, capsules, powder in vials or ampules). The dose unit relates to the capsule, the tablet, sachet, lozenge as well as to the packaging containing a sufficient number of unit doses.

The subject of the invention also relates to a method for the production of the above medical preparations, in such a way that the idelalisib base Form E according to the invention is mixed with medically suitable solid or liquid diluents and/or excipients, and the mixture is placed into a galenic form.

The medical preparations according to the invention are produced using the usual methods of pharmaceutical production. If necessary, the medical preparations according to the invention may also contain further medical active substances with the compounds or a mixture of compounds according to the invention. The subject of the invention also relates to the use of the compounds according to the present invention as a medical active substance.

The subject of the present invention relates to the use of the idelalisib base Form E according to the invention for the production of a medical preparation serving for the treatment of cancer diseases. The subject of the invention also relates to the use of the idelalisib base Form E according to the invention on its own or in combination with the active substance rituximab for the treatment of cancer diseases. The treated cancer disease may include leukaemia, preferably chronic lymphocytic leukaemia or follicular lymphoma.

The advantage of the invention is that the compounds according to the invention are substances with uniform morphology and preferable crystalline forms. Accordingly, these substances have preferable and reproducible characteristics in terms of dissolution rate, bioavailability, chemical stability, hygroscopicity and processability (filtration features, drying, tableting, etc.).

The active substances according to the invention may be advantageously produced using reproducible processes at industrial scales.

EXAMPLES

Measurement conditions used

Dynamic vapour sorption analysis (DVS)

SMS DVS Advantage DVSA1-STD dynamic vapour sorption

Device:

analyser

Atmosphere: nitrogen

Total gas flow rate: 200 mL/min

Solvent: water

Temperature: 25 °C

Regulation: Open cycle

Step size: 5 % RH

Stability criterion: 0.002 %/min Phases: DMDT Window: 5 min

Minimum stability time 30 min

Maximum phase time: 360 min Data saving frequency: 1 min

Measurement range: 0% RH→ 85% RH

X-ray powder diffraction measurement conditions

In the case of all crystalline forms presented here the x-ray diffraction data of the new idelalisib base forms (and their various solvates) according to our invention were obtained under the following measurement conditions:

Device: PANalytical Empyrean X-ray powder diffractometer Sampling: Transmission

X-ray

Type: Empyrean Long Fine Focus High Resolution tube

Anode: Cu

Wavelength: Ka (1.541874 A)

Optics

Divergence slit: Fixed slit 1/2 °

Mirror: Focussing elliptical mirror

Soller slit: 0.04 rad

Diffusion inhibitor slit: Fixed slit 1/2 °

Diffraction ray optics

Diffusion inhibitor slit: Programmable slit in fixed mode: 1/2

Soller slit: 0.04 rad

Sample table

Type: Reflection-transmission spinner stage

Sample rotation: 1 rotation/min Detector

Type: PrXcel 3D 1 x 1 area detector Mode: Scanning line detector (ID) operation mode

Active length: 3.3473°

Sample preparation: unpowdered samples placed between Mylar sheets

Measurement settings

Temperature: room temperature

Accelerating voltage: 45 kV

Anode heating current: 40 mA

Scanning: continuous gonio (Θ/Θ) scan

Measurement range: 2.0000-34.9964° 2Θ

Step gap: 0.0131° 2Θ

Step duration: 109.650 s

Measurement cycles: 1

Measurement time: -20 min

Conditions of the pressing tests

The resistance against mechanical stresses of the crystal structure of the new idelalisib base form according to our invention and of the polymorphs known from the literature was studied using pressing tests. Tablets were formed from the powder samples using a Specac Atlas 15T hydraulic press with a Star pressure gauge (15 tonne). The pressure applied was approx. 50 MPa. The crystal structure of the tablets produced was studied without powdering using x-ray powder diffraction, under the give measurement conditions.

Further details of the solution according to our invention are presented in the following examples without restricting the scope of protection of the invention in any way to the examples.

Example 1: The production of the idelalisib base Form E-S acetonitrile solvate (method "A")

A mixture of 16.4 g (49.13 mmol) (5)-2-(l-amino-propyl)-5-fluor-3-phenyl-quinazolin- 4(3H)-one hydrochloride (II), 9.9 g (63.9 mmol) 6-chloropurine, 330 ml isopropyl alcohol (IPA) and 20.5 ml ( 14.9 g; 147.4 mmol) triethylamine (TEA) at an internal temperature of 82- 84 °C is stirred for 63 hours. The orange coloured solution is cooled to 45-50 °C, then evaporated in a vacuum (50 °C, 30-35 mbar). The evaporation residue is dissolved in 200 ml of dichloromethane (DCM), then washed in 2x 50 ml of water. The organic phase is dried with 10 g of magnesium sulphate (stirred for 1 hour), and cleared with 0.6 g of active carbon. The drying agent and clearing carbon are filtered out, then washing is performed with 2x20 ml of DCM, the solvent is removed in a vacuum. 100 ml of acetonitrile (ACN) is added to the residue resin-type material at room temperature. A homogenous solution is obtained after stirring, and a few minutes after continued stirring the crystallization of the idelalisib Form E- S acetonitrile solvate starts. After two hours of stirring the precipitated material is filtered, and then washed with small amounts of ACN being added to cover the substance until the filtrate becomes colourless (approx. 6x 5 ml). The material obtained is the acetonitrile solvate of idelalisib (Form E-S).

Example 2: The production of the idelalisib base Form E-S acetonitrile solvate (method "B")

A mixture of 16.4 g (49.1 mmol) (5)-2-(l-amino-propyl)-5-fluor-3-phenyl-quinazolin-4(3H)- one hydrochloride (II), 9.9 g (63.9 mmol) 6-chloropurine, 330 ml isopropyl alcohol (IPA) and 20.5 ml (14.9 g; 147.4 mmol) triethylamine (TEA) at an internal temperature of 82-84 °C is stirred for 63 hours. The orange coloured solution is cooled to 45-50 °C, then evaporated in a vacuum (50 °C, 30-35 mbar) so that 77 g of mixture remains in the flask. It is cooled to room temperature, 1 g of clearing carbon is added which its filtered out along with the precipitated TEA HQ (approx. 10 g), the filtered substance is washed with 10 ml of IPA. 60 ml of water is added to the solution. The mixture is brought to the boil and further water is added drop by drop in order to obtain a clear solution. Leave to cool. Crystallization starts at about 40 °C. After room temperature is reached the solution is stirred for 2 hours, the precipitated substance is filtered out and then washed with an IPA:water = 1: 1 mixture, then dried for 5 hours at 100 °C in a vacuum drying cabinet. 7 ml/g of ACN is added to the crystalline material obtained. After stirring a homogenous solution is obtained, which after continued stirring starts to crystalize after a few minutes. After two hours of stirring the precipitated material is filtered, and then washed with small amounts of ACN being added to cover the substance (approx. 3 x 15 ml). The material obtained is the acetonitrile solvate of idelalisib (Form E-S).

Example 3: The production of the idelalisib base (I) Form E polymorph

(I)

The idelalisib Form E-S acetonitrile solvate obtained in either of examples 1 or 2 is dried in a drying cabinet at 100 °C for 15 hours. Form E idelalisib polymorph is obtained as an off- white crystalline material.

Mp: 208-210 °C.

Element analysis: calculated: C22H18FN7O (415.43): C 63.61%, H 4.37%, N 23.60%; measured: C 63.51%, H 4.28%; N 23.09%