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Title:
NEW PROTEIN DEAMIDASE
Document Type and Number:
WIPO Patent Application WO/2015/133590
Kind Code:
A1
Abstract:
Provided are: a new protein deamidase that directly acts on side chain amide groups of asparagine residues in proteins, and that has the effect of disassociating side chain carboxyl groups and ammonia; a micro-organism producing said protein deamidase; a gene coding said protein; and a manufacturing method and application therefor. Bacteria that is classified in the actinobacteria class are cultured and made to produce protein deamidase, and the enzyme is harvested from the cultured product.

Inventors:
YOKOYAMA, Noriko (INC. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-sh, Kanagawa 81, 〒2108681, JP)
NAKANO, Mayu (INC. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-sh, Kanagawa 81, 〒2108681, JP)
HASHIRO, Shuhei (INC. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-sh, Kanagawa 81, 〒2108681, JP)
HIRAGA, Masako (INC. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-sh, Kanagawa 81, 〒2108681, JP)
SHIMBO, Kazutaka (INC. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-sh, Kanagawa 81, 〒2108681, JP)
TOKUNAGA, Ayaka (INC. 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-sh, Kanagawa 81, 〒2108681, JP)
Application Number:
JP2015/056569
Publication Date:
September 11, 2015
Filing Date:
March 05, 2015
Export Citation:
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Assignee:
AJINOMOTO CO., INC. (15-1, Kyobashi 1-chome Chuo-k, Tokyo 15, 〒1048315, JP)
International Classes:
C12N15/09; A21D2/26; A23C9/13; A23C13/16; A23L1/00; A23L1/24; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/78; C12P21/02
Foreign References:
JP2001218590A2001-08-14
Other References:
M. KIKUCHI ET AL.: "Peptidoasparaginase. An Enzyme for Deamidation of COOH-Terminal Peptide-Bound Asparagine", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 148, no. 1, 1972, pages 315 - 317, XP024808446, ISSN: 0003-9861
A. KATO ET AL.: "Effects of Deamidation with chymotrypsin at pH 10 on the Functional Properties of Proteins", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 35, no. 2, 1987, pages 285 - 288, XP055222868
WEINSTOCK, G. ET AL.: "PXTG-motif protein cell wall anchor domain protein [Leifsonia aquatica ATCC 14665", NCBI, 2013, XP055357987, Retrieved from the Internet [retrieved on 20150527]
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (Acropolis 21 Building 6th floor, 4-10 Higashi Nihonbashi 3-chome, Chuo-k, Tokyo 04, 〒1030004, JP)
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