NEW USE OF TAXOID DERIVATIVES The present invention relates a new use of taxoid derivatives. It relates more precisely a method for treating abnormal cell proliferation in the brain of mammals including men by administrating a compound of general formula (I) or a pharmaceutically acceptable salt or solvante thereof: inwhich: Z represents a hydrogen atom or a radical of general formula: in which: R, represents a benzoyl radical optionally substituted with one or more identical or different atoms or radicals chosen from halogen atoms and alkyl radicals containing 1 to 4 carbon atoms, alkoxy radicals containing I to 4 carbon atoms or trifluoromethyl radicals, a thenoyl or furoyl radical or a radical R,-O-CO-in which R represents : -an alkyl radical containing 1 to 8 carbon atoms, -an alkenyl radical containing 2 to 8 carbon atoms, -an alkynyl radical containing 3 to 8 carbon atoms, -a cycloalkyl radical containing 3 to 6 carbon atoms,

-a cycloalkenyl radical containing 4 to 6 carbon atoms or -a bicycloalkyl radical containing 7 to 10 carbon atoms, these radicals being optionally substituted with one or more substituents chosen from halogen atoms and hydroxyl radicals, alkoxy radicals containing 1 to 4 carbon atoms, dialkylamino radicals in which each alkyl portion contains I to 4 carbon atoms, piperidino or morpholino radicals, I-piperazinyl radicals (optionally substituted at position 4 with an alkyl radical containing 1 to 4 carbon atoms or with a phenylalkyl radical in which the alkyl portion contains 1 to 4 carbon atoms), cycloalkyl radicals containing 3 to 6 carbon atoms, cycloalkenyl radicals containing 4 to 6 carbon atoms, phenyl radicals (optionally substituted with one or more atoms or radicals chosen from halogen atoms and alkyl radicals containing 1 to 4 carbon atoms or alkoxy radicals containing I to 4 carbon atoms), cyano or carboxyl radicals or alkoxycarbonyl radicals in which the alkyl portion contains I to 4 carbon atoms, -a phenyl or a-or p-naphthyl radical optionally substituted with one or more atoms or radicals chosen from halogen atoms and alkyl radicals containing 1 to 4 carbon atoms or alkoxy radicals containing 1 to 4 carbon atoms, or -a 5-membered aromatic heterocyclic radical preferably chosen from furyl and thienyl radicals, -or a saturated heterocyclic radical containing 4 to 6 carbon atoms, optionally substituted with one or more alkyl radicals containing 1 to 4 carbon atoms, R3 represents an unbranched or branched alkyl radical containing 1 to 8 carbon atoms, an unbranched or branched alkenyl radical containing 2 to 8 carbon atoms, an unbranched or branched alkynyl radical containing 2 to 8 carbon atoms, a cycloalkyl radical containing 3 to 6 carbon atoms, a phenyl or a-or p-naphthyl radical optionally substituted with one or more atoms or radicals chosen from halogen atoms and alkyl, alkenyl, alkynyl, aryl, aralkyl, alkoxy, alkylthio, aryloxy, arylthio, hydroxyl, hydroxyalkyl, mercapto, formyl, acyl, acylamino, aroylamino, alkoxycarbonylamino, amino, alkylamino,

dialkylamino, carboxyl, alkoxycarbonyl, carbamoyl, alkylcarbamoyl, dialkylcarbamoyl, cyano, nitro and trifluoromethyl radicals, or a 5-membered aromatic heterocycle containing one or more identical or different hetero atoms chosen from nitrogen, oxygen and sulphur atoms and optionally substituted with one or more identical or different substituents chosen from halogen atoms and alkyl, aryl, amino, alkylamino, dialkylamino, alkoxycarbonylamino, acyl, arylcarbonyl, cyano, carboxyl, carbamoyl, alkylcarbamoyl, dialkylcarbamoyl or alkoxycarbonyl radicals, on the understanding that, in the substituents of the phenyl, a-or p-naphthyl and aromatic heterocyclic radicals, the alkyl radicals and the alkyl portions of the other radicals contain I to 4 carbon atoms, and that the alkenyl and alkynyl radicals contain 2 to 8 carbon atoms, and that the aryl radicals are phenyl or a-or p-naphthy ! radicals, R4 represents an alkoxy radical containing 1 to 6 carbon atoms in an unbranched or branched chain, an alkenyloxy radical containing 3 to 6 carbon atoms in an unbranched or branched chain, an alkynyloxy radical containing 3 to 6 carbon atoms in an unbranched or branched chain, a cycloalkyloxy radical containing 3 to 6 carbon atoms or a cycloalkenyloxy radical containing 4 to 6 carbon atoms, these radicals being optionally substituted with one or more halogen atoms or with an alkoxy radical containing I to 4 carbon atoms, an alkylthio radical containing 1 to 4 carbon atoms or a carboxyl radical, an alkyloxycarbonyl radical in which the alkyl portion contains 1 to 4 carbon atoms, a cyano or carbamoyl radical or an N-alkylcarbamoyl or N, N-dialkylcarbamoyl radical in which each alkyl portion contains 1 to 4 carbon atoms or, with the nitrogen atom to which it is linked, forms a saturated 5-or 6-membered heterocyclic radical optionally containing a second hetero atom chosen from oxygen, sulphur or nitrogen atoms, optionally substituted with an

alkyl radical containing 1 to 4 carbon atoms or a phenyl radical or a phenylalkyl radical in which the alkyl portion contains 1 to 4 carbon atoms, R5 represents an alkoxy radical containing 1 to 6 carbon atoms in an unbranched or branched chain, an alkenyloxy radical containing 3 to 6 carbon atoms, an alkynyloxy radical containing 3 to 6 carbon atoms, a cycloalkyloxy radical containing 3 to 6 carbon atoms or a cycloalkenyloxy radical containing 3 to 6 carbon atoms, these radicals being optionally substituted with one or more halogen atoms or with an alkoxy radical containing 1 to 4 carbon atoms, an alkylthio radical containing 2 to 4 carbon atoms or a carboxyl radical, an alkyloxycarbonyl radical in which the alkyl portion contains I to 4 carbon atoms, a cyano or carbamoyl radical or an N-alkylcarbamoyl or N, N-dialkylcarbamoyl radical in which each alkyl portion contains 1 to 4 carbon atoms or, with the nitrogen atom to which it is linked, forms a saturated 5-or 6-membered heterocyclic radical optionally containing a second hetero atom chosen from oxygen, sulphur or nitrogen atoms, optionally substituted with an alkyl radical containing 1 to 4 carbon atoms or a phenyl radical or a phenylalkyl radical in which the alkyl portion contains I to 4 carbon atoms.

Preferably, the aryl radicals which can be represented by R3 are phenyl or a- or p-naphthyl radicals optionally substituted with one or more atoms or radicals chosen from halogen atoms (fluorine, chlorine, bromine, iodine) and alkyl, alkenyl, alkynyl, aryl, arylalkyl, alkoxy, alkylthio, aryloxy, arylthio, hydroxyl, hydroxyalkyl, mercapto, formyl, acyl, acylamino, aroylamino, alkoxycarbonylamino, amino, alkylamino, dialkylamino, carboxyl, alkoxycarbonyl, carbamoyl, dialkylcarbamoyl, cyano, nitro and trifluoromethyl radicals, on the understanding that the alkyl radicals and the alkyl portions of the other radicals contain 1 to 4 carbon atoms, that the alkenyl and alkynyl radicals contain 2 to 8 carbon atoms and that the aryl radicals are phenyl or a-or p-naphthyl radicals.

Preferably, the heterocyclic radicals which can be represented by R3 are 5-membered aromatic heterocyclic radicals containing one or more identical or different atoms chosen from nitrogen, oxygen and sulphur atoms, optionally substituted with one or more identical or different substituents chosen from halogen atoms (fluorine, chlorine, bromine, iodine) and alkyl radicals containing 1 to 4 carbon atoms, aryl radicals containing 6 to 10 carbon atoms, alkoxy radicals containing I to 4 carbon atoms, aryloxy radicals containing 6 to 10 carbon atoms, amino radicals, alkylamino radicals containing 1 to 4 carbon atoms, dialkylamino radicals in which each alkyl portion contains 1 to 4 carbon atoms, acylamino radicals in which the acyl portion contains 1 to 4 carbon atoms, alkoxycarbonylamino radicals containing 1 to 4 carbon atoms, acyl radicals containing I to 4 carbon atoms, arylcarbonyl radicals in which the aryl portion contains 6 to 10 carbon atoms, cyano, carboxyl or carbamoyl radicals, alkylcarbamoyl radicals in which the alkyl portion contains 1 to 4 carbon atoms, dialkylcarbamoyl radicals in which each alkyl portion contains I to 4 carbon atoms or alkoxycarbonyl radicals in which the alkoxy portion contains 1 to 4 carbon atoms.

Preferably, the radicals R4 and R5, which may be identical or different, represent unbranched or branched alkoxy radicals containing 1 to 6 carbon atoms, optionally substituted with a methoxy, ethoxy, ethylthio, carboxyl, methoxycarbonyl, ethoxycarbonyl, cyano, carbamoyl, N-methylcarbamoyl, N-ethylcarbamoyl, N, N-dimethylcarbamoyl, N, N-diethylcarbamoyl, N-pyrrolidinocarbonyl or N-piperidinocarbonyl radical.

More specially, the present invention relates to the products of general formula (I) in which Z represents a hydrogen atom or a radical of general formula (II) in which R, represents a benzoyl radical or a radical R2-O-CO-in which R2 represents a tert-butyl radical and R3 represents an alkyl radical containing 1 to 6 carbon atoms, an alkenyl radical containing 2 to 6 carbon atoms, a cycloalkyl radical containing 3 to 6 carbon atoms, a phenyl radical optionally substituted with one or more identical or different atoms or radicals chosen from halogen atoms (fluorine, chlorine) and alkyl (methyl), alkoxy (methoxy), dialkylamino (dimethylamino), acylamino

(acetylamino), alkoxycarbonylamino (tert-butoxycarbonylamino) or trifluoromethyl radicals, or a 2-or 3-furyl, 2-or 3-thienyl or 2-, 4-or 5-thiazolyl radical, and R4 and R5, which may be identical or different, each represent an unbranched or branched alkoxy radical containing 1 to 6 carbon atoms.

Still more specially, the present invention relates to the products of general formula (I) in which Z represents a hydrogen atom or a radical of general formula (II) in which R, represents a benzoyl radical or a radical R,-O-CO-in which R, represents a tert-butyl radical and R3 represents an isobutyl, isobutenyl, butenyl, cyclohexyl, phenyl, 2-furyl, 3-furyl, 2-thienyl, 3-thienyl, 2-thiazolyl, 4-thiazolyl or 5-thiazolyl radical, and R4 and R5, which may be identical or different, each represent a methoxy, ethoxy or propoxy radical.

Of even more special interest are the products of general formula (I) in which R3 represents a phenyl radical and R, represents a ter-butoxycarbonyl radical, R4 and R5, which may be identical or different, represent a methoxy, ethoxy or propoxy radical.

In a still higher interest, the present invention relates to 4a-acetoxy-2a- benzoyloxy-5p, 20-epoxy-1 P-hydroxy-7 ?, 1 Op-dimethoxy-9-oxo-11-taxen-13a-yl (2R, 3S)-3 tert-butoxycarbonylamino-2-hydroxy-3-phenylpropionate of formula (Ia) It is known, from patent WO 96/30355, to prepare a derivative according to the present invention by two processes. According to a first, multi-step process, starting with 10-deacetylbaccatin III of formula:

it is selectively protected in positions 7 and 13, for example in the form of a silyl diether, followed by the action of a product of general formula: R-X (IV) in which R represents a radical as defined above and X represents a reactive ester residue such as a sulphuric or sulphonic ester residue or a halogen atom, to give a product bearing the unit-OR in position 10 and silyl groups in positions 7 and 13.

Next, the silyl protecting groups are replaced with hydrogen atoms to give a compound still bearing the group-OR in position 10 and OH groups in positions 7 and 13. The latter derivative is etherified selectively in position 7 by reaction with the derivative of formula IV to give the derivative of formula (I) in which Z is equal to hydrogen.

The final step consists in esterifying in position 13, according to a process which is known per se, the derivatives of formula (Ia), in which Z represents hydrogen, in the presence of a P-lactam according, for example, to the process described in patent EP 617,018, or in the presence of an oxazolidine as described, for example, in patent WO 96/30355 mentioned above. After deprotection of the protecting groups in positions 7 and 10, an ester of formula (Ia) is thus obtained in which Z is other than hydrogen and R represents hydrogen. The next step consists in reacting the positions 7 and 10 simultaneously by the action of a reagent formed

in situ from a sulphoxide of formula (V) and acetic anhydride (Pummerer-type reaction), R-SO-R (V) in which R has the same meaning as above, to form an alkylthioalkyloxy-type intermediate on positions 7 and 10.

The final step, which allows the desired compound of formula (Ia) to be obtained, is carried out on the intermediate compound obtained above, by the action of activated Raney nickel.

Generally, the action of the reagent formed in situ from sulphoxide of general formula (V), preferably dimethyl sulphoxide and acetic anhydride, is carried out in the presence of acetic acid or an acetic acid derivative such as a haloacetic acid, at a temperature of between 0 and 50°C.

Generally, the action of the activated Raney nickel in the presence of an aliphatic alcohol or an ether is carried out at a temperature of between-10 and 60°C.

In the FR 97-14442 application a further process has been described. This invention allows, in a single step, the direct, selective and simultaneous alkylation of the two hydroxyl functions in positions 7 and 10 of 10-deacetylbaccatin or of derivatives thereof esterified in position 13, of formula (VI) in which A represents hydrogen or a side chain of formula (IIa) below: in which G represents a protecting group for the hydroxyl function, R, and R3 have the same meaning as in formula (II) or an oxazolidine unit of formula (Id):

in which R, and R3 have the same meaning as in formula (II), Ra and Rb, which may be identical or different, represent hydrogen or alkyl, aryl, halo, alkoxy, arylalkyl, alkoxyaryl, haloalkyl, haloaryl, it being possible for the substituents optionally to form a 4-to 7-membered ring.

It is preferred to use 10-deacetylbaccatin as starting material, i. e. the product of formula (III), which allows appreciable economy as regards the process and moreover avoids the intermediate protection and deprotection steps necessary in the old processes.

Among the groups G for protecting the hydroxyl function of formula (IIa), it is generally preferred to choose all of the protecting groups described in books such as Greene and Wuts, Protective Groups in Organic Synthesis, 1991, John Wiley & Sons, and MacOmie, Protective Groups in Organic Chemistry, 1975, Plenum Press, and which are deprotected under conditions which degrade the rest of the molecule little or not at all, such as, for example: * ethers, and preferably ethers such as methoxymethyl ether, 1-ethoxyethyl ether, benzyloxymethyl ether, p-methoxybenzyloxymethyl ether, benzyl ethers optionally substituted with one or more groups such as methoxy, chloro, nitro, 1-methyl-

1-methoxyethyl ether, 2- (trimethylsilyl) ethoxymethyl ether, tetrahydropyranyl ether and silyl ethers such as trialkylsilyl ethers, carbonates such as trichloroethyl carbonates.

More particularly, the radicals Ra and Rb of general formula (IIb) are chosen from those described in patent WO 94/07878 and the derivatives more particularly preferred are those in which Ra is hydrogen and Rb is a p-methoxyphenyl radical.

The alkylating agent is chosen from: * alkyl halides, and preferably from alkyl iodides (RI) * alkyl sulphates such as methyl sulphate * oxoniums such as trialkyloxonium boric salts, in particular trimethyloxonium tetrafluoroborate (Me, OBF4).

Methyl iodide is preferably used.

The alkylating agent is used in the presence of an anionization agent such as one or more strong bases, in anhydrous medium.

Among the bases which can be used in anhydrous medium, mention may be made of: * alkali metal hydrides such as sodium or potassium hydride * alkali metal alkoxides such as potassium tert-butoxide * silver oxide Ag2O * 1,8-bis (dimethylamino) naphthalene * mono-or dimetallic base mixtures such as those described, for example, in publications such as P. Caubere Chem. Rev. 1993,93,2317-2334 or M. Schlosser Mod. Synth. Methods (1992), 6,227-271; in particular the alkyllithium/alkali metal t-butoxide or alkali metal amide/alkali metal t-butoxide combinations are preferred.

One of the two bases can be generated"in situ".

Among all of the possible combinations of alkylating agent and anionization agent, it is preferred to use methyl iodide in the presence of potassium hydride.

The reaction is preferably carried out in an organic medium which is inert under the reaction conditions. Among the solvents, it is preferred to use : ethers such as tetrahydrofuran or dimethoxyethane * when silver oxide is used, it is preferred to use polar aprotic solvents such as dimethylformamide, or aromatic solvents such as toluene * when 1,8-bis (dimethylamino) naphthalene is used, it is preferred to use alkylesters such as ethylacetate.

For better implementation of the invention, it is preferred to use a molar ratio between the anionization agent and the substrate of greater than 2 and preferably between 2 and 20.

It is also preferred to use a molar ratio between the alkylating agent and the substrate of greater than 2 and preferably between 2 and 40.

It is preferred to use a reaction temperature of between-30°C and 80°C.

The reaction time advantageously ranges between a few hours and 48 hours depending on the reagents chosen.

After the alkylating step, when the latter is carried out on 10-deacetylbaccatin, the process then proceeds, in a known manner, to the esterification step according, for example, to the processes described in patents EP 617,018 or WO 96/30355 mentioned above.

Thus, according to a first, 3-step process, the procedure first begins with the dialkylation of 10-deacetylbaccatin, using an alkylating agent in the presence of a strong base, in a second step, the 10-deacetylbaccatin dietherified in positions 7 and 10 is coupled, in position 13, with a suitably protected p-Iactam in the presence of an activating agent chosen from tertiary amines and metal bases which ensure the formation of an alkoxide in position 13. Deprotection of the side chain is then achieved by the action of an inorganic or organic acid.

Thus, according to a second, 3-step process, the procedure first begins with the dialkylation of 10-deacetylbaccatin, using an alkylating agent in the presence of a

strong base, in a second step, the 10-deacetylbaccatin dietherified in positions 7 and 10 is coupled, in position 13, with an oxazolidine in the presence of a coupling agent such as diimides in the presence of an activating agent such as dialkylaminopyridines.

Opening of the oxazolidine is achieved by the action of an inorganic or organic acid.

According to a third process, the procedure begins with the esterification in position 13 of baccatin suitably protected in positions 7 and 10, with a ß-lactam or an oxazolidine in the presence of a coupling agent and/or an activating agent as described in the above two processes. After deprotection in positions 7 and 10, the dietherification in positions 7 and 10 is carried out by an alkylating agent in the presence of a strong base. Deprotection of the side chain is then achieved by the action of an inorganic or organic acid.

The products of general formula (I) have remarkable biological properties.

In vitro, measurement of the biological activity is carried out on tubulin extracted from pig brain by the method of M. L. Shelanski et al., Proc. Natl. Acad. Sci.

USA, 70,765-768 (1973). The study of the depolymerization of the microtubules into tubulin is carried out according to the method of G. Chauviere et al., C. R. Acad. Sci., 293, série II, 501-503 (1981).

In vivo, the products of general formula (I) proved active in mice grafted with the B 16 melanoma at doses of berween I and 50 mg/kg intraperitoneally, as well as on other liquid or solid tumours.

The compounds have anti-tumor properties, more particularly, activity against tumors which are resistant to Taxon and Taxotere@. Such tumors include, for example, brain tumors which have an elevated expression of mdr 1 gene (multi- drug resistant gene). Multi-drug resistance is the usual term relating to the resistance by a tumor against various compounds having differing structures and mechanisms of action. Taxoids are generally known to be highly recognized by experimental tumors such as P388/DOX, a P388 murine leukemia cell line selected for doxorubicin (DOX) resistance, which express mdr 1. The compounds according to the present invention

are less recognized by P388/DOX. More particularly, the compounds are less recognized than Taxotere by mdr 1.

The compounds of formula (I) are mainly used for preparing a medecine for treating abnormal cell proliferation in the brain.

The compound and mainly compound fo formula (I) where R4 and Rs are each methoxy has the property to cross the blood brain barrier. It is active compared to the other known taxoids such as Taxon or Taxotere to treat the brain cancer.

The product of formula (I) can be used concurrently with at least other therpaeutic treatment. It is more preferably used with other therapeutic treatment comprising antineoplastic drugs, monoclonal antibodies, immunotherapies, radiotherapies, or biological response modifiers. Among the biological responses modifier lymphokines and cytokines, interleukins, a, ß, or 8 interfeons and TNF are preferably used.

The product of formula (I) is preferably administered by parenteral administration such as intravenous, intraperitoneal, intramuscular or subcutaneous administration.

Example 1 1. INTRODUCTION Product of formula (Ia) is a potent anti-cancer agent in pre-clinical models.

Reported here are the analytical results obtained from a single i. v. bolus pharmacokinetic study in the mouse.

Groups of female C3H/HeN mice received the product by the intravenous route as a bolus at a dose level of 40 mg. kg'equivalent to 120 mg. m~2. Blood and brain samples were obtained from all dosed animals sacrificed at intervals up to 72 hours post dose.

Brain and corresponding plasma samples have been assayed for product la content by an LC-MS/MS assay.

2. METHODS mg. ml-' solution containing 5 % Polysorbate 80, 5 % ethanol and 90 % of an aqueous 5 % glucose solution.

Fifty-six female C3H/HeN mice each weighing ca 20 g were each administered formulated product II by i. v. bolus via the tail vein at an injection volume of 0.4 ml to give a total dose of 40 mg. kg'.

Blood and Tissue Sampling Sampling: Blood by cardiac puncture and liver and brain by dissection after CO, sacrifice Sample Times: at 2,5,15,30,45 minutes, 1,2,4,6,8,14,24,48 and 72 hours post dose.

Whole blood was collected into heparinised tubes and corresponding plasma samples were obtained by centrifugation and frozen immediately at-20°C. Tissues were blotted, weighed and frozen immediately at-20°C. All samples were dispatched frozen for analysis. Upon receipt, samples were stored frozen at approximately-18°C pending analysis.

LC-MS/MS Analysis of Brain and Plasma Samples Detection: LC-MS/MS (Sciex API III plus) in turbo-ionspray mode The following MS conditions were applied: Auxiliary gas flow 6 L. min' Nebuliser gas flow 0.6 L. min' Turbo temperature 450°C CGT 300 Curtain gas flow 0.6 L. min' Scan time 1 scan/sec Eluent split ratio 1: 10 Column: 75 x 4.6 mm Supelcosil ABZ plus (3 um).

Mobile phase: Acetonitrile/methanol/ammonium acetate (lOmM); 40/25/35 v/v/v.

Flow rate: 1 ml. min-1.

Temperature: Ambient.

Add 100 ptl of acetonitrile to sample (50 ul). Vortex, centrifuge, remove supernatant, add 100 ul of mobile phase and inject 150p1.

Brain: Add 100 pi ouf acetonitrile to homogenised sample (100 mg of a 1: 1 w/w brain with water homogenate) and vortex. Add 1 ml of diethyl ether, vortex, centrifuge, remove organic layer and dry under N2.

Reconstitute in 200 1 of mobile phase and inject 150 p1.

Calibration standards: Plasma: Nine at concentrations of 5,10,20,50,100,200,300, 400 & 500 ng. mol-l (product Ia). Prepared by adding suitable aliquots of the product (concentrations = 0.1,1 or 10 llg. ml-l) in ethanol to 0.5 ml aliquots of mouse plasma. Each sample was vortexed following drug addition; a 50 pl aliquot was then removed for assay.

Brain: Eleven at concentrations of 10,20,30,100,200,300,400,500, 1000,2500 & 5000 ng. g-in homogenised mouse brain (1: 1 w/w brain with water). Prepared by adding suitable aliquots of the product (concentrations = l, 10 or 100 ug. ml'') in ethanol to or 4 g of homogenised mouse brain. Each sample was vortexed following drug addition and a 100 mg aliquot was then removed for assay.

Retention times: Drug; product la:-2.3 min.

Extraction ca. 58 % at 200 ng. mol".

Brain: ca. 41 % at 500 ng. g' and 39 % at 1000 ng. g'.

3. RESULTS 3.1 Plasma Levels The following table contains product la plasma levels observed after i. v. administration of product Ic at a dose level of 40 mg. kg''to the mouse. Table 1 Preliminary plasma concentrations of product la after i. v. dosing at a level of 40 mg. ks'' to the mouse Time after Plasma product Ic concentration (ng. ml-') dose (h) IV1 IV2 IV3 IV4 MEAN + s. d. 2 min 52987 45942 49607 38994 46882 5994 5 min 36734 33538 32077 34903 34313 1984 15 min 20493 20897 21051 19459 20475 717 0.5h 10765 10344 9170 11232 10378 883 0.75h 7133 10948 8121 10148 9087 1764 lh 6017 7423 6693 6079 6553 655 46334337460035644283498 4h 1072 1110 835 830 962 150 6h 449 316 346 336 362 59 8h 204 199 195 154 188 23 14h 65 56 50 52 56 7 24 18 (blq) 15 (blq) 15 (blq) 16 (blq) 16 1 48 4 (blq) n. d. n. d. 4 (blq) 2 2 72 n. d. n. d. n. d. n. d.---n. a.

n. a.: not applicable n. d.: not detected (< l. o. d. of 4 ng. mur') blq: below limit of accurate quantification (20 ng. ml-').

3.2 Brain Levels The following table contains product la whole brain levels observed after i. v. administration of product la at a dose level of 40 mg. kg' to the mouse.

Table 2 Preliminary brain concentration for product Ic after i. v. dosing at a level of 40 mWg. kg-l to the mouse Time after Brain product Ic concentration (ng. g) dose (h) IV1 IV2 IV3 IV4 MEAN + s. d. 2 min 6962 8817 8147 7630 7889 786 5 min 8344 8473 7762 8091 8167 313 15 min 5809 7100 7641 6481 6758 791 0.5h 7262 6788 8317 6894 7315 698 0.75h 7675 8086 7513 7272 7637 342 lh 6424 8964 1747 7489 6156 3118 2h 7956 8418 6966 7017 7589 716 4h 7909 6939 6712 5459 6755 1008 6h 6688 7968 7350 3712 6430 1886 8h 9067 6977 8616 8342 8250 900 14h 9618 10049 7595 9271 9133 1074 24 7905 9842 7885 9052 8671 952 48 6660 8541 7704 7986 7723 789 72 5899 5511 5692 3894 5249 917

n. d.: not detected (<I. o. d. of 92 ng. g') n. a.: not applicable 3.3 Pharmacokinetic Parameters The following table contains the preliminary pharmacokinetic parameters for product Ic derived after i. v. administration to the mouse at 40 mg. kg-l calculated using mean plasma and brain level data.

Table 3 Preliminary mean pharmacokinetic data Sample AUCo-W CIT Vdss Initial Terminal (h. g. ml-'or. g') (l. h-'. kg') (l. kg') Tn/2 Tn2 (h) (h) Plasma+ 1.9 0. 7 6.2 Plasma# 29.8 1 1.3 2.4 0. 2 2. 0 Brain 787.8* n. a. n. a.-31.4 + calculated from values > l. o. d. of 4 ng. ml-'

# calculated from values ! b. l. q. of 20 ng. ml~' * the corresponding AUCo-72h = 549.7 h. g. g-' A biexponential equation was fitted to the profiles using an interactive linear least- square algorithm as part of the SIPHAR package. AUC was calculated by the trapezoidal rule from time 0 to both the time of the last value that was equal to or greater than the l. o. d. + (4 ng. mol'') or the l. o. q. # (20 ng. ml-') for plasma and up to 72h post dose for the brain, and then extrapolated to infinity.

AUCo cO: Area under the plasma or brain concentration versus time curve from t=0 (start of infusion) to infinity.

Initial T,: Initial (distribution) half-life.

Terminal T,,, : Terminal (elimination) half-life (should be regarded as an estimate only being dependent on sampling frequency in the terminal phase and assay sensitivity).

CIT: Total plasma clearance.

Vdss: Volume of distribution at steady state. n. a.: Not applicable.

4. CONCLUSIONS Product Ic levels were high as would be expected after an i. v. dose of 40 mg. ka but declined rapidly from the peak at 2 minutes (mean of 46.9 pg. ml-1) to less than 1 pg. ml~ within 4h (initial half-life of < 7h). However However persisted above the limit of accurate quantification (20 ng. ml~}) up to 14h post dose and consistently above the limit of detection (4 ng. ml~l) for up to 24h post dose.

A terminal half-life of 6.2 h was calculated from detectable plasma levels (24 ng. mol''). However, it should be noted that the terminal half-life is very dependent on assay sensitivity in this case and if levels above the limit of accurate quantification (20 ng. ml~l) are utilised to calculate pharmacokinetic parameters instead, then the terminal half-life drops to 2. Oh.

Mean total plasma clearance was determined to be 1.3 l. h-l. kg-l which represents a significant fraction of average liver plasma flow (based on average liver blood flow of ca 5.2 l. h-l. kg-l).

In this species post-i. v. administration, product Ic appears to readily penetrate the blood brain barrier. High levels were detected at the first sampling time (7.9 pg g-l at 2 mins) indicating rapid uptake into this tissue. Although peak levels of 9.1 pg. g~ 'were observed at 14h, high concentrations were sustained up to the last sampling time (5.3 g. g~ at 72h). Not surprisingly the product is slowly cleared from the brain with a half-life of 31.4h. On the basis of AUCo-values (788 h. ug. g-l versus 30 h. g. mol-1), product la levels in the brain were about twenty times those in the plasma.

Example 2 Evaluation of product (IA) for Antitumor Activity Against Intracranially Implanted Human Glioblastomas U251 and SF-295 in NCr-nu Mice.

Four studies were initiated to evaluate the response of U251 and SF-295 glioblastomas to treatment with product (Ia). In the two studies, U251 and SF-295 glioblastomas were initiated from intracranially implanted cells at a volume of 106 cells per mouse. The treatment schedule of the intracranially implanted U251 glioblastoma cells was iv, once daily, every sixth day for three treatments (q6d x 3), beginning on day four postimplant. The treatment schedule of the intracranially implanted SF-295 glioblastoma cells was iv, once daily, every fourth day for three treatments (q4d x 3), beginning on day two postimplant. For the intracranially implanted studies, the compounds were evaluated based on their ability to increase the life span of the animals. The positive control used for both of these tumor models was nitrosourea.

The objective of this experimentation was to evaluate the product (Ia) for antitumor effect against human glioblastoma tumor models.

In these experiments, general DCTD, NCI techniques and procedures for in vivo efficacy studies were modified for special application (In Vivo Cancer Models, NIH Publication No. 84-2635,1984) : These studies were conducted in approved facilities (AAALAC Registration No. 000643, AALAS Membership No. 840723001, USDA Registration No. 64-R-001, OPPR, PHS, NIH, AWA, Assurance No. A3046-01).

These facilities are ISO 9001 certifie. The oversight committee was the Southern Research Institutional Animal Care and Use Committee; the protocol used was IACUC No. 96-8-50.

Dilutions: Product (Ia) was prepared in 5 % ethanol, 5 % tween 80,90 % D5W Nitrosourea was prepared in 2 % ethanol, 98 % physiological saline.

Dose Preparation: All dosing solutions were prepared at Southern Research Institute.

Compound Administration: Product (Ia) was administered in 0.4 ml/mouse based on a total body weight average.

Nitrosourea was administered 0.1 ml/10 g of body weight.

Compound Stability: Product (Ia) was kept on ice and administered within 20 minutes of preparation.

Nitrosourea was kept on ice and administered within 45 minutes of preparation.

Storage Conditions: All compounds were stored in refrigerated desiccators.

Handling Precautions: The compounds were handled according to procedures required by the Safety Committee of Southern Research Institute. All technicians were fully gowned and gloved with face mask and safety glasses during compound administration.

Any intracranially implanted animal that appeared to be moribund was euthanized for humane purposes. Since efficacy studies fall within this catagory of basic research, experiment termination was based on resultats that were determined to be optimal.

Species: Six to eight week old athymic NCr-nu female mice were used for the intracranially implanted U251 trials. Six to eight week old athymic NCr-nu male mice were used for the intracranially implanted SF-295 trials.

Justification: Immune deficient mice are necessary for the propagation of human tumor xenografts, which was the target tissue for the compounds being developed.

Source: FCRDC (Animal Production Area), Frederick, MD for intracranially implanted SF-295 trial; Taconic Animal Farms, Germantown, NY for intracranially implanted U251 trials.

Number and Sex: A total of 160 males were used on the intracranially implanted SF- 295 trials; a total of 154 females were used on the intracranially implanted U251 trials.

Weight and age: Mean weights were taken at the time each trial was initiated. The mean weight of the mice implanted intracranially with U251 gliobastoma was 21 to 22 g. The mean weight of the mice implanted intracranially with SF-295 glioblastoma was 24to 26 g.

Animal Identification: Standard ear marks.

Quarantine: All animals were held for a seven-day observation period before being put on test.

Housing and Sanitation: The animals were housed in filter-capped isolator cages, five per cage. The cages and bedding were changed twice weekly.

Food and Water: Teklad Sterlizable 8656 Mouse Diet (Harlan Teklad) was given ad libitum. Filtered tap water was provided ad libitum.

Environmental Conditions: Maintained according to SRI standard operating procedures approved by the IACUC committee.

Two experiments were involved in this study (RP-36 and RP-38).

As previously mentioned, this experimentation was designed to evaluate the activity of product (Ia) against intracranially U251 and SF-295 glioblastomas in athymic MCr-nu mice. The dosages for product (Ia) were 30,20 and 13.4 mg/kg/dose. For the two intracranially implanted experiments, cells were prepared at a concentration of 3.33 x 107 cells per ml of media and injected at a volume of 0.03 ml per mouse. The cells were injected into the cerebrum to the right of the midline with a 25 guage, 3/8 inch, stainless steel needle. Cultured cells were used for the U251 experiment (RP-36). The treatment schedule was q6d x 3, iv, beginning on day four, postimplant.

A tumor brei, made from solid tumor, was used for the SF-295 experiment (RP-38).

The treatment schedule was q4d x 3, iv, beginning on day two, postimplant.

Nitrosourea was given in each experiment for comparative purposes because of its known activity against CNS tumors. The dosages were 27,18 and 12 mg/kg/dose and the treatment schedule was the same as the treatment schedule for product (Ia) in each experiment.

In the first experiment (RP-36), each compound was effective in the treatment of intracranially implanted U251 glioblastoma. Treatment with product (Ia) resulted in five of ten, four of ten and three of ten 122-day survivors and an ILS of 176 %, 202 % and 144 % respectively, for the dosage groups of 30,20 (MTD) and 13.4 mg/kg/dose.

Treatment with nitrosourea resulted in an ILS of 205 % and 51 % in the dosage groups of 18 and 12 mg/kg/dose, respectively. There were ten of ten and seven of ten 122-day survivors in the dosage groups of 27 (MTD) and 18 mg/kg/dose.

In the second experiment (RP-38), each compound was effective in the treatment of intracranially implanted SF-295 glioblastoma. Treatment with product (Ia) at 30,20 and 13.4 (MTD) mg/kg/dose resulted in an ILS of-9 %, 94 % and 81 %, respectively.

There was some toxicity at the dosage levels of 30 and 20 mg/kg/dose as evidenced by a respective 7 g and 6 g mean weight loss through the treatment period. There was one 68-day survivor out of ten animals in the dosage group of 13.4 mg/kg/dose.

Nitrosourea was toxic at the highest dosage level of 27 mg/kg/dose as evidenced by a

7 g mean weight loss through the treatment period. Treatment with nitrosourea at dosages of 27,18 and 12 mg/kg/dose resulted in an ILS of 50 %, 131 % and 106 %, respectively. There were two 68-day survivors out of ten animals at the dosage level of 27 (MTD) mg/kg/dose, and there was one 68-day surviror out of ten animals at the dosage level of 18 mg/kg/dose.

In summary, product (Ia) was tested against both intracranially implanted U251 and SF-295 glioblastomas. This compound was quite active against these two tumor lines at both implant sites.

RESPONSE OF IC IMPLANTED U251 GLIOBLASTOMA TO TREATMENT WITH PRODUCT (la ! Group Treatment: IV, Q6D X 3 (4) Days22-day Median % ILS surv./total day of death I Agent control Dosage 26 31 31 34 34 34 34 34 34 34 1/20 34.0 (treated) (mg/kg/dose) 34 37 37 39 41 43 45 80 115- 8 Product I (a) 30.0 21 34 94 98 103----------5/10 94.0 +176 S 9 20.0 58 98 100 106 106 122--------4/10 103.0 +202 10 13.4 69 69 83 83 83 83 122------3/10 83.0 +144 S S 14 Nitrosourea 27.0--------------------10/10 15 18.0 94 104 104--------------7/10 104.0 +205 16 12.0 41 45 49 49 49 54 66 69 69 80 0/10 51.5 +51 S <BR> U251 Glioblastoma; tumor source: cell culture; implanted: 04/17/98; evaluation date: 08/17/98; Athymic NCr-nu<BR> mice-female-taconic farms<BR> Control, 2 % EtOH/saline; injection volume = 0.1 cc/10 g body weight<BR> Product I (a), batch BFC611, prepared from lot no. 1 in 5 % EtOH/5 % tween 80/90 % D5W (soluble);<BR> injection volume = 0.4 cc<BR> Nitrosourea prepared from lot no. 2 in 2 % EtOH/saline (soluble); injection volume = 0.1 cc/10 g body weight<BR> Note 1) 122-Day survivors not used in calculations. Medians calculated by using all deaths.<BR> <P> 2) S = sacrified, animal moribund (used in calculations).

RESPONSE OF IC IMPLANTED SF-295 GLIOBLASTOMA TO TREATMENT WITH PRODUCT (la) GroupTreatinent : IV, Q4D X 3 (2) Days of death 68-day Median % # surv./total day of ILS death 1 Agent control Dosage 10 13 14 14 14 14 15 15 16 16 0/20 16.0 (treated) (mg/kg/dose) 16 17 17 18 18 18 19 20 20 20 8 Product I (a) 30.0 11 13 13 14 14 15 30 40 41 47 0/10 14.5-9 9 20.0 13 13 16 31 31 31 33 33 37 54 0/10 31.0 +94 10 13.4 25 25 29 29 29 30 32 34 41--1/10 29.0 +81 14 Nitrosourea 27.0 15 16 16 19 29 37 56 64----2/10 24.0 +50 s 15 18.0 33 34 34 35 37 37 37 40 41--1/10 37.0 +131 16 12.0 22 23 26 26 33 33 33 33 33 40 0/10 33.0 +106 <BR> SF-295 Glioblastoma; tumor source: 01/A/05F3T8; implanted: 08/26/98; Athymic MCr-nu Mice-Male-Frederick Cancer<BR> Research Development Center<BR> Control, 2 % EtOH/Saline; injection volume = 0.1 cc/10 g body weight<BR> Product I (a), batch BFC611, prepared from SRI lot n°. 1 in 5 % EtOH/5 % Tween 80/90 % D5W (soluble);<BR> injection volume = 0.4 cc<BR> Nitrosourea, Bristol-Myers lot LAH84, prepared from SRI lot n° 2-4 in 2 % EtOH/Saline (soluble); injection volume = 0.1 cc/10 g<BR> body weight<BR> Note: 1) 68-day survivors not used in calculations. Medians calculated by using all deaths.<BR> <P> 2) S = sacrificed due to paralysis (used in calculations).