Title:
NON-DESTRUCTIVE MEASUREMENT, DISPENSE, AND REPLICATION OF DENSITY GRADIENTS
Document Type and Number:
WIPO Patent Application WO/2024/025807
Kind Code:
A3
Abstract:
A system performs a non-destructive measurement of a density gradient to automatically replicate and dispense the density gradient. The system obtains measurements at points along a length of the density gradient and generates a profile of the density gradient based on the measurements. The system uses the profile to replicate the density gradient of components in a second container. The system inserts a distal end of a probe into the second container, and pumps separate components into a manifold and mixing chamber connected to a proximal end of the probe to automatically dispense the density gradient in the second container.
Inventors:
MCINTYRE LARRY (US)
HALSMER MATTHEW (US)
KIBLER STEVEN (US)
HALSMER MATTHEW (US)
KIBLER STEVEN (US)
Application Number:
PCT/US2023/028403
Publication Date:
March 28, 2024
Filing Date:
July 21, 2023
Export Citation:
Assignee:
BECKMAN COULTER INC (US)
International Classes:
G01N9/24; G01N9/30
Domestic Patent References:
| WO2022102258A1 | 2022-05-19 |
Foreign References:
| JPH10311775A | 1998-11-24 | |||
| CN111397841A | 2020-07-10 |
Other References:
VERHEUL ROSS: "How can I prepare consistent density gradients for reproducibly purifying samples via ultracentrifugation?", BECKMAN.COM/ SPINSIGHTS, 005, BECKMAN COULTER LIFE SCIENCES, 1 May 2020 (2020-05-01), XP055929612
LEIF ET AL: "Density gradient system - II. A 50 channel programmable undulating diphragm peristaltic pump", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 25, 1 January 1968 (1968-01-01), pages 283 - 296, XP024826156, ISSN: 0003-2697, [retrieved on 19680101], DOI: 10.1016/0003-2697(68)90103-6
ENGMANN ANNE K ET AL: "Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 17, no. 2, 12 January 2022 (2022-01-12), pages 222 - 251, XP037686751, ISSN: 1754-2189, [retrieved on 20220112], DOI: 10.1038/S41596-021-00638-7
LEIF ET AL: "Density gradient system - II. A 50 channel programmable undulating diphragm peristaltic pump", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 25, 1 January 1968 (1968-01-01), pages 283 - 296, XP024826156, ISSN: 0003-2697, [retrieved on 19680101], DOI: 10.1016/0003-2697(68)90103-6
ENGMANN ANNE K ET AL: "Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 17, no. 2, 12 January 2022 (2022-01-12), pages 222 - 251, XP037686751, ISSN: 1754-2189, [retrieved on 20220112], DOI: 10.1038/S41596-021-00638-7
Attorney, Agent or Firm:
BRUESS, Steven, C. (US)
Download PDF:
Previous Patent: MOTION FEEDBACK USING PATTERNED FLOWCELLS
Next Patent: POPULATION-SCALE ORGANOID POOLS
Next Patent: POPULATION-SCALE ORGANOID POOLS